{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:14:54Z","timestamp":1762899294222},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T00:00:00Z","timestamp":1471996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0161169","type":"journal-article","created":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T17:48:05Z","timestamp":1472060885000},"page":"e0161169","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge"],"prefix":"10.1371","volume":"11","author":[{"given":"Lucilene Aparecida","family":"Resende","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Dian de Oliveira","family":"Aguiar-Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique","family":"Gama-Ker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Mendes","family":"Roatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludmila Zanandreis de","family":"Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Luiza Rodrigues","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise da","family":"Silveira-Lemos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"Corr\u00eaa-Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olindo Assis","family":"Martins-Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio Sobreira Silva","family":"Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Toshio","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelder Figueiredo","family":"Gontijo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre Barbosa","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo Cordeiro","family":"Giunchetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,8,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S0169-4758(00)01637-9","article-title":"The strange case of <italic>Leishmania chagasi<\/italic>","volume":"16","author":"IL Maur\u00edcio","year":"2000","journal-title":"Parasitol Today"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.cimid.2004.03.004","article-title":"Leishmaniasis: current situation and new perspectives","volume":"27","author":"P Desjeux","year":"2004","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e313","DOI":"10.1371\/journal.pntd.0000313","article-title":"Complexities of assessing the disease burden attributable to leishmaniasis","volume":"2","author":"C Bern","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"287","DOI":"10.4269\/ajtmh.1995.52.287","article-title":"Control of zoonotic visceral leishmaniasis: is it time to change strategies?","volume":"52","author":"RB Tesh","year":"1995","journal-title":"Am J Trop Med Hyg"},{"key":"ref5","first-page":"161","article-title":"Reservoirs of <italic>Leishmania donovani<\/italic> in Brazil","volume":"7","author":"LM Deane","year":"1961","journal-title":"Rev Assoc Med Bras"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S1471-4922(02)02347-4","article-title":"Canine leishmaniasis: epidemiological risk and experimental model","volume":"18","author":"J Moreno","year":"2002","journal-title":"Trends Parasitol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S1090-0233(97)80006-8","article-title":"Studies of canine leucocyte antigens: a significant advance in canine immunology","volume":"153","author":"DL Williams","year":"1997","journal-title":"The Vet J"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.rvsc.2005.09.011","article-title":"Parasite density and impaired biochemical\/hematological status are associated with severe clinical aspects of canine visceral leishmaniasis","volume":"81","author":"AB Reis","year":"2006","journal-title":"Res Vet Sci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.vetimm.2006.02.001","article-title":"Isotype patterns of immunoglobulins: hallmarks for clinical status and tissue parasite density in Brazilian dogs naturally infected by <italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>chagasi<\/italic>","volume":"112","author":"AB Reis","year":"2006","journal-title":"Vet Immunol Immunopathol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2249.2006.03206.x","article-title":"Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by <italic>Leishmania chagasi<\/italic>","volume":"146","author":"AB Reis","year":"2006","journal-title":"Clin Exp Immunol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jcpa.2006.06.005","article-title":"Relationship between canine visceral leishmaniosis and the <italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>chagasi<\/italic> burden in dermal inflammatory foci","volume":"135","author":"RC Giunchetti","year":"2006","journal-title":"J Comp Pathol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.vetimm.2006.10.001","article-title":"Analysis of the cytokine profile in spleen cells from dogs naturally infected by <italic>Leishmania chagasi<\/italic>","volume":"115","author":"RS Lage","year":"2007","journal-title":"Vet Immunol Immunopathol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.vetimm.2007.07.009","article-title":"Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis","volume":"121","author":"RC Giunchetti","year":"2008","journal-title":"Vet Immunol Immunopathol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.rvsc.2007.04.020","article-title":"Histopathological and immunohistochemical investigations of the hepatic compartment associated with parasitism and serum biochemical changes in canine visceral leishmaniasis","volume":"84","author":"RC Giunchetti","year":"2008","journal-title":"Res Vet Sci"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.vetpar.2010.01.023","article-title":"Relationship of <italic>Leishmania<\/italic>-specific IgG levels and IgG avidity with parasite density and clinical signs in canine leishmaniasis","volume":"169","author":"RG Teixeira Neto","year":"2010","journal-title":"Vet Parasitol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e1566","DOI":"10.1371\/journal.pntd.0001566","article-title":"Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis","volume":"6","author":"D Menezes-Souza","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e82947","DOI":"10.1371\/journal.pone.0082947","article-title":"Clinical forms of canine visceral Leishmaniasis in naturally <italic>Leishmania infantum<\/italic>-infected dogs and related myelogram and hemogram changes","volume":"23","author":"R de C Nicolato","year":"2013","journal-title":"PLoS One"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"125","DOI":"10.4269\/ajtmh.1996.55.125","article-title":"The logic of visceral leishmaniasis control","volume":"55","author":"C Dye","year":"1996","journal-title":"Am Trop Med Hyg"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4223","DOI":"10.1016\/j.vaccine.2007.02.083","article-title":"Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: Double-blind randomised efficacy field trial","volume":"25","author":"JL Lemesre","year":"2007","journal-title":"Vaccine"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"7674","DOI":"10.1016\/j.vaccine.2007.08.009","article-title":"Immunogenicity of a killed <italic>Leishmania<\/italic> vaccine with saponin adjuvant in dogs","volume":"25","author":"RC Giunchetti","year":"2007","journal-title":"Vaccine"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"5888","DOI":"10.1016\/j.vaccine.2008.05.095","article-title":"Protective immunity against challenge with <italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>chagasi<\/italic> in beagle dogs vaccinated with recombinant A2 protein","volume":"26","author":"AP Fernandes","year":"2008","journal-title":"Vaccine"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.vaccine.2007.11.057","article-title":"A killed <italic>Leishmania<\/italic> vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs","volume":"265","author":"RC Giunchetti","year":"2008","journal-title":"Vaccine"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.rvsc.2007.09.008","article-title":"Antigenicity of a whole parasite vaccine as promising candidate against canine leishmaniasis","volume":"85","author":"RC Giunchetti","year":"2008","journal-title":"Res Vet Sci"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"3716","DOI":"10.1016\/j.vaccine.2005.02.009","article-title":"Protective vaccination against experimental canine visceral leishmaniasis using a combination of DNA and protein immunization with cysteine proteinases type I and II of <italic>L<\/italic>. <italic>infantum<\/italic>","volume":"23","author":"S Rafati","year":"2005","journal-title":"Vaccine"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e49780","DOI":"10.1371\/journal.pone.0049780","article-title":"Performance of LBSap vaccine after intradermal challenge with <italic>L<\/italic>. <italic>infantum<\/italic> and saliva of <italic>Lu<\/italic>. <italic>longipalpis<\/italic>: immunogenicity and parasitological evaluation","volume":"7","author":"BM Roatt","year":"2012","journal-title":"PLoS One"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.vetpar.2013.09.011","article-title":"Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with <italic>Leishmania chagasi<\/italic> plus saliva of <italic>Lutzomyia longipalpis<\/italic>","volume":"198","author":"LA Resende","year":"2013","journal-title":"Vet Par"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1756-3305-7-61","article-title":"LbSapSal-vaccinated dogs exhibit increased circulating T-lymphocyte subsets (CD4+ and CD8+) as well as a reduction of parasitism after challenge with <italic>Leishmania infantum<\/italic> plus salivary gland of <italic>Lutzomyia longipalpis<\/italic>","volume":"7","author":"RD Aguiar-Soares","year":"2014","journal-title":"Parasit Vectors"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3277","DOI":"10.1016\/S0264-410X(02)00294-3","article-title":"Long lasting protection against canine kala-azar using the FML-QuilA saponin vaccine in an endemic area of Brazil (S\u00e3o Gon\u00e7alo do Amarante, RN)","volume":"20","author":"GP Borja-Cabrera","year":"2002","journal-title":"Vaccine"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1016\/j.vaccine.2008.02.044","article-title":"Despite Leishvaccine and Leishmune trigger distinct immune profiles, their ability to activate phagocytes and CD8+ T-cells support their high-quality immunogenic potential against canine visceral leishmaniasis","volume":"26","author":"MS Ara\u00fajo","year":"2008","journal-title":"Vaccine"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1016\/j.vaccine.2008.11.104","article-title":"T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-<italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>chagasi<\/italic> IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune","volume":"27","author":"MS Ara\u00fajo","year":"2009","journal-title":"Vaccine"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.vetimm.2005.03.001","article-title":"Lymphocytes of dogs immunised with purified excreted-secreted antigens of <italic>Leishmania infantum<\/italic> coincubated with <italic>Leishmania<\/italic> infected macrophages produce IFN gamma resulting in nitric oxide-mediated amastigote apoptosis","volume":"106","author":"P Holzmuller","year":"2005","journal-title":"Vet Immunol Immunopathol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e1683","DOI":"10.1371\/journal.pntd.0001683","article-title":"Use of a LiESP\/QA-21 vaccine (CaniLeish) stimulates an appropriate Th1- dominated cell-mediated immune response in dogs","volume":"6","author":"J Moreno","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1126\/science.3344436","article-title":"Salivary gland lysates from the sand fly <italic>Lutzomyia longipalpis<\/italic> enhance <italic>Leishmania<\/italic> infectivity","volume":"239","author":"RG Titus","year":"1988","journal-title":"Science"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1084\/jem.173.1.49","article-title":"A mouse model of <italic>Leishmania braziliensis<\/italic> braziliensis infection produced by coinjection with sand fly saliva","volume":"173","author":"J Samuelson","year":"1991","journal-title":"J Exp Med"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1098\/rstb.1994.0097","article-title":"Saliva of <italic>Lutzomyia longipalpis<\/italic> sibling species differs in its composition and capacity to enhance leishmaniasis","volume":"345","author":"A Warburg","year":"1994","journal-title":"Philos Trans R Soc B"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"5442","DOI":"10.1128\/IAI.64.12.5442-5445.1996","article-title":"Effects of sand fly vector saliva on development of cutaneous lesions and the immune response to <italic>Leishmania braziliensis<\/italic> in BALB\/c mice","volume":"64","author":"HC Lima","year":"1996","journal-title":"Infect Immun"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1084\/jem.188.10.1941","article-title":"Development of a natural model of cutaneous leishmaniasis: powerful effects of vector saliva and saliva pre-exposure on the long-term outcome of <italic>Leishmania major<\/italic> infection in the mouse ear dermis","volume":"188","author":"Y Belkaid","year":"1998","journal-title":"J Exp Med"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1126\/science.290.5495.1351","article-title":"Protection against cutaneous leishmaniasis resulting from bites of uninfected sand flies","volume":"290","author":"S Kamhawi","year":"2000","journal-title":"Science"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"e84","DOI":"10.1371\/journal.pntd.0000084","article-title":"Enhanced <italic>Leishmania braziliensis<\/italic> infection following pre-exposure to sandfly saliva","volume":"1","author":"TR de Moura","year":"2007","journal-title":"PLoS Negl Trop Dis"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"7845","DOI":"10.1073\/pnas.0712153105","article-title":"Immunity to a salivary protein of a sand fly vector protects against the fatal outcome of visceral leishmaniasis in a hamster model","volume":"105","author":"R Gomes","year":"2008","journal-title":"Proc Natl Acad Sci"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e1000441","DOI":"10.1371\/journal.ppat.1000441","article-title":"Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for <italic>Leishmania<\/italic>","volume":"5","author":"N Collin","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"OH Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","article-title":"Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids","volume":"126","author":"LC Green","year":"1982","journal-title":"Anal Biochem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1093\/clinchem\/38.10.2152","article-title":"Simple, rapid method for determining nitrates and nitrites in biological fluids","volume":"38","author":"SI Gutman","year":"1992","journal-title":"Clin Chem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1128\/CDLI.8.4.828-831.2001","article-title":"Real-time PCR as a new tool for quantifying <italic>Leishmania infantum<\/italic> in liver in infected mice","volume":"8","author":"S Bretagne","year":"2001","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e47907","DOI":"10.1371\/journal.pone.0047907","article-title":"Parasite burden in hamsters infected with two different strains of <italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>infantum<\/italic>: &quot;Leishman Donovan units&quot; versus real-time PCR","volume":"7","author":"Moreira Nd","year":"2012","journal-title":"PLoS One"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/00034983.1995.11813015","article-title":"Experimental models for leishmaniasis and for testing anti-leishmanial vaccines","volume":"89","author":"M Hommel","year":"1995","journal-title":"Ann Trop Med Parasitol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.pt.2010.04.005","article-title":"Immunity to <italic>Leishmania<\/italic> and the rational search for vaccines against canine leishmaniasis","volume":"26","author":"AB Reis","year":"2010","journal-title":"Trends Parasitol"},{"key":"ref49","first-page":"69","article-title":"Vaccines for canine leishmaniasis","volume":"3","author":"CB Palatnik-de-Sousa","year":"2012","journal-title":"Front Immunol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.vetimm.2008.11.020","article-title":"Expression of IFN-gamma, TNF-alpha, IL-10 and TGF-beta in lymph nodes associates with parasite load and clinical form of disease in dogs naturally infected with <italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>chagasi<\/italic>","volume":"128","author":"CF Alves","year":"2009","journal-title":"Vet Immunol Immunopathol"},{"key":"ref51","first-page":"541","article-title":"Cytokine and phenotypic cell profiles of <italic>Leishmania infantum<\/italic> infection in the dog","volume":"2012","author":"C Maia","year":"2011","journal-title":"J Trop Med"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1179\/1364859411Y.0000000027","article-title":"Evaluation of TNF-\u03b1, IL-4, and IL-10 and parasite density in spleen and liver of <italic>L<\/italic>. (<italic>L<\/italic>.) <italic>chagasi<\/italic> naturally infected dogs","volume":"105","author":"A DE F Michelin","year":"2011","journal-title":"Ann Trop Med Parasitol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1016\/j.vaccine.2013.01.048","article-title":"Induction of immunogenicity by live attenuated <italic>Leishmania donovani<\/italic> centrin deleted parasites in dogs","volume":"31","author":"JA Fiuza","year":"2013","journal-title":"Vaccine"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.it.2007.07.004","article-title":"Interleukin-10 and the pathogenesis of human visceral leishmaniasis","volume":"28","author":"S Nylen","year":"2007","journal-title":"Trends Immunol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0165-2427(99)00048-3","article-title":"Detection of canine cytokine gene expression by reverse transcription-polymerase chain reaction","volume":"69","author":"E Pinelli","year":"1999","journal-title":"Vet Immunol Immunopathol"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0165-2427(02)00134-4","article-title":"Cytokine expression during the outcome of canine experimental infection by <italic>Leishmania infantum<\/italic>","volume":"88","author":"GM Santos-Gomes","year":"2002","journal-title":"Vet Immunol Immunopathol"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1128\/IAI.66.3.1233-1236.1998","article-title":"Transforming growth factor beta and immunosuppression in experimental visceral leishmaniasis","volume":"66","author":"JR Virmondes-Rodrigues","year":"1998","journal-title":"Infect Immun"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.0105-2896.2004.00188.x","article-title":"The immunopathology of experimental visceral leishmaniasis","volume":"201","author":"PM Kaye","year":"2004","journal-title":"Immunol Rev"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.4049\/jimmunol.170.5.2613","article-title":"Activation of TGF-beta by <italic>Leishmania chagasi<\/italic>: importance for parasite survival in macrophages","volume":"170","author":"KR Gantt","year":"2003","journal-title":"J Immunol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1128\/IAI.62.1.229-235.1994","article-title":"Cellular and humoral immune responses in dogs experimentally and naturally infected with <italic>Leishmania infantum<\/italic>","volume":"62","author":"E Pinelli","year":"1994","journal-title":"Infect Immun"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1016\/j.vaccine.2006.10.036","article-title":"Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis","volume":"25","author":"E Carrillo","year":"2007","journal-title":"Vaccine"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.vetpar.2010.11.025","article-title":"Cytokine and transcription factor profiles in the skin of dogs naturally infected by <italic>Leishmania<\/italic> (<italic>Leishmania<\/italic>) <italic>chagasi<\/italic> presenting distinct cutaneous parasite density and clinical status","volume":"177","author":"D Menezes-Souza","year":"2001","journal-title":"Vet Parasitol"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1017\/S0031182009006155","article-title":"Cytokine expression in dogs with natural <italic>Leishmania infantum<\/italic> infection","volume":"136","author":"MA Panaro","year":"2009","journal-title":"Parasitology"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1084\/jem.169.1.59","article-title":"Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murine leishmaniasis. Evidence for 642 expansion of distinct helper T cell subsets","volume":"169","author":"FP Heinzel","year":"1989","journal-title":"J Exp Med"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ijpara.2004.10.015","article-title":"Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in <italic>Leishmania infantum<\/italic>-infected dogs","volume":"35","author":"D Strauss-Ayali","year":"2005","journal-title":"Int J Parasitol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.4049\/jimmunol.143.12.4244","article-title":"Experimental visceral leishmaniasis: role of endogenous IFN-gamma in host defense and tissue granulomatous response","volume":"143","author":"KE Squires","year":"1989","journal-title":"J Immunol"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.4049\/jimmunol.148.6.1858","article-title":"Acquired resistance and granuloma formation in experimental visceral leishmaniasis. Differential T cell and lymphokine roles in initial versus established immunity","volume":"148","author":"HW Murray","year":"1992","journal-title":"J Immunol"},{"key":"ref68","first-page":"809","article-title":"Evaluation of the immune response and production of interferon in canine visceral leishmaniasis","volume":"50","author":"HM Andrade","year":"1999","journal-title":"Revue Med Vet"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1365-3024.2006.00807.x","article-title":"The immunomodulatory factors of arthropod saliva and the potential for these factors to serve as vaccine targets to prevent pathogen transmission","volume":"28","author":"RG Titus","year":"2006","journal-title":"Parasite Immunol"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4269\/ajtmh.2005.72.94","article-title":"Inflammatory cell infiltration and high antibody production in BALB\/c mice caused by natural exposure to <italic>Lutzomyia longipalpis<\/italic> bites","volume":"72","author":"F Silva","year":"2005","journal-title":"Am J Trop Med Hyg"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0304-4017(03)00132-8","article-title":"A follow-up of Beagle dogs intradermally infected with <italic>Leishmania chagasi<\/italic> in the presence or absence of sand fly saliva","volume":"114","author":"M Paranhos-Silva","year":"2003","journal-title":"Vet Parasitol"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"e60535","DOI":"10.1371\/journal.pone.0060535","article-title":"Experimental infection of dogs with <italic>Leishmania<\/italic> and saliva as a model to study canine visceral leishmaniasis","volume":"8","author":"DJ Costa","year":"2013","journal-title":"PLoS One"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0147-9571(00)00026-6","article-title":"Nitric oxide production by macrophages of dogs vaccinated with killed <italic>Leishmania infantum<\/italic> promastigotes","volume":"24","author":"MA Panaro","year":"2001","journal-title":"Comp Immunol Microbiol Infect Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0161169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T01:36:21Z","timestamp":1718760981000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0161169"}},"subtitle":[],"editor":[{"given":"Sima","family":"Rafati","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,8,24]]},"references-count":73,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2016,8,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0161169","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,24]]}}}