{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:23:15Z","timestamp":1791310995919,"version":"4.3.1"},"reference-count":30,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T00:00:00Z","timestamp":1471478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA31534"],"award-info":[{"award-number":["R01CA31534"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"CPRIT","doi-asserted-by":"crossref","award":["RP120348, 120459"],"award-info":[{"award-number":["RP120348, 120459"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006975","name":"Indiana CTSI","doi-asserted-by":"crossref","award":["Core Pilot Fund"],"award-info":[{"award-number":["Core Pilot Fund"]}],"id":[{"id":"10.13039\/100006975","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Arid3a and Arid3b belong to a subfamily of ARID (AT-rich interaction domain) transcription factors. The Arid family is involved in regulating chromatin accessibility, proliferation, and differentiation. Arid3a and Arid3b are closely related and share a unique REKLES domain that mediates their homo- and hetero-multimerization. Arid3a was originally isolated as a B cell transcription factor binding to the AT rich matrix attachment regions (MARS) of the immunoglobulin heavy chain intronic enhancer. Deletion of Arid3a results in a highly penetrant embryonic lethality with severe defects in erythropoiesis and hematopoietic stem cells (HSCs). The few surviving <jats:italic>Arid3a<\/jats:italic><jats:sup>-\/-<\/jats:sup> (&lt;1%) animals have decreased HSCs and early progenitors in the bone marrow, but all mature lineages are normally represented in the bone marrow and periphery except for B cells. <jats:italic>Arid3b<\/jats:italic><jats:sup><jats:italic>-\/-<\/jats:italic><\/jats:sup> animals die around E7.5 precluding examination of hematopoietic development. So it is unclear whether the phenotype of Arid3a loss on hematopoiesis is dependent or independent of Arid3b. In this study we circumvented this limitation by also examining hematopoiesis in mice with a conditional allele of Arid3b. Bone marrow lacking Arid3b shows decreased common lymphoid progenitors (CLPs) and downstream B cell populations while the T cell and myeloid lineages are unchanged, reminiscent of the adult hematopoietic defect in Arid3a mice. Unlike <jats:italic>Arid3a<\/jats:italic><jats:sup><jats:italic>-\/-<\/jats:italic><\/jats:sup> mice, HSC populations are unperturbed in <jats:italic>Arid3b<\/jats:italic><jats:sup><jats:italic>-\/-<\/jats:italic><\/jats:sup> mice. This study demonstrates that HSC development is independent of Arid3b, whereas B cell development requires both Arid3a and Arid3b transcription factors.<\/jats:p>","DOI":"10.1371\/journal.pone.0161468","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T17:41:04Z","timestamp":1471542064000},"page":"e0161468","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Arid3b Is Critical for B Lymphocyte Development"],"prefix":"10.1371","volume":"11","author":[{"given":"Jeffrey L.","family":"Kurkewich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Klopfenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Hallas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel A.","family":"Sattler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chhaya","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haley","family":"Tucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Dahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen D.","family":"Cowden Dahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,8,18]]},"reference":[{"key":"pone.0161468.ref001","first-page":"315","article-title":"Recent advances in the ARID family: focusing on roles in human cancer","volume":"7","author":"C Lin","year":"2014","journal-title":"OncoTargets and therapy"},{"issue":"10","key":"pone.0161468.ref002","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1038\/ni1008-1091","article-title":"The Immunological Genome Project: networks of gene expression in immune cells","volume":"9","author":"Immunological Genome Project C","year":"2008","journal-title":"Nat Immunol"},{"issue":"21","key":"pone.0161468.ref003","doi-asserted-by":"crossref","first-page":"15768","DOI":"10.1074\/jbc.M700397200","article-title":"REKLES is an ARID3-restricted multifunctional domain","volume":"282","author":"D Kim","year":"2007","journal-title":"J Biol Chem"},{"issue":"24","key":"pone.0161468.ref004","doi-asserted-by":"crossref","first-page":"3067","DOI":"10.1101\/gad.9.24.3067","article-title":"The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family","volume":"9","author":"RF Herrscher","year":"1995","journal-title":"Genes Dev"},{"issue":"15","key":"pone.0161468.ref005","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1093\/emboj\/19.15.4123","article-title":"Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies","volume":"19","author":"RT Zong","year":"2000","journal-title":"EMBO J"},{"issue":"10","key":"pone.0161468.ref006","doi-asserted-by":"crossref","first-page":"4747","DOI":"10.4049\/jimmunol.160.10.4747","article-title":"Expression of bright at two distinct stages of B lymphocyte development","volume":"160","author":"CF Webb","year":"1998","journal-title":"J Immunol"},{"issue":"5","key":"pone.0161468.ref007","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1128\/MCB.01448-10","article-title":"The ARID family transcription factor bright is required for both hematopoietic stem cell and B lineage development","volume":"31","author":"CF Webb","year":"2011","journal-title":"Mol Cell Biol"},{"issue":"6","key":"pone.0161468.ref008","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1242\/dev.057570","article-title":"Apical ectodermal ridge morphogenesis in limb development is controlled by Arid3b-mediated regulation of cell movements","volume":"138","author":"JC Casanova","year":"2011","journal-title":"Development"},{"issue":"1","key":"pone.0161468.ref009","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ydbio.2005.12.016","article-title":"Microarray analysis of PDGFR alpha+ populations in ES cell differentiation culture identifies genes involved in differentiation of mesoderm and mesenchyme including ARID3b that is essential for development of embryonic mesenchymal cells","volume":"293","author":"A Takebe","year":"2006","journal-title":"Dev Biol"},{"issue":"21","key":"pone.0161468.ref010","doi-asserted-by":"crossref","first-page":"4168","DOI":"10.1242\/dev.109918","article-title":"Arid3b is essential for second heart field cell deployment and heart patterning","volume":"141","author":"V Uribe","year":"2014","journal-title":"Development"},{"issue":"3","key":"pone.0161468.ref011","doi-asserted-by":"crossref","first-page":"976","DOI":"10.4049\/jimmunol.125.3.976","article-title":"An in vitro line of the B cell tumor BCL1 can be activated by LPS to secrete IgM1","volume":"125","author":"ES Gronowicz","year":"1980","journal-title":"J Immunol"},{"issue":"6","key":"pone.0161468.ref012","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1128\/MCB.26.6.2187-2201.2006","article-title":"A regulated nucleocytoplasmic shuttle contributes to Bright's function as a transcriptional activator of immunoglobulin genes","volume":"26","author":"D Kim","year":"2006","journal-title":"Mol Cell Biol"},{"issue":"7","key":"pone.0161468.ref013","doi-asserted-by":"crossref","first-page":"e42159","DOI":"10.1371\/journal.pone.0042159","article-title":"ARID3B induces tumor necrosis factor alpha mediated apoptosis while a novel ARID3B splice form does not induce cell death","volume":"7","author":"S Joseph","year":"2012","journal-title":"PLoS One"},{"issue":"5","key":"pone.0161468.ref014","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1093\/nar\/11.5.1475","article-title":"Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei","volume":"11","author":"JD Dignam","year":"1983","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"pone.0161468.ref015","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1182\/blood-2014-06-579813","article-title":"A crucial role for the homeodomain transcription factor Hhex in lymphopoiesis","volume":"125","author":"JT Jackson","year":"2015","journal-title":"Blood"},{"issue":"5","key":"pone.0161468.ref016","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1084\/jem.173.5.1213","article-title":"Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow","volume":"173","author":"RR Hardy","year":"1991","journal-title":"J Exp Med"},{"issue":"5","key":"pone.0161468.ref017","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/S0092-8674(00)80453-5","article-title":"Identification of clonogenic common lymphoid progenitors in mouse bone marrow","volume":"91","author":"M Kondo","year":"1997","journal-title":"Cell"},{"issue":"20","key":"pone.0161468.ref018","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1101\/gad.1836009","article-title":"Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development","volume":"23","author":"MA Inlay","year":"2009","journal-title":"Genes Dev"},{"issue":"17","key":"pone.0161468.ref019","doi-asserted-by":"crossref","first-page":"9797","DOI":"10.1073\/pnas.96.17.9797","article-title":"Functional characterization of B lymphocytes generated in vitro from embryonic stem cells","volume":"96","author":"SK Cho","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"9","key":"pone.0161468.ref020","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1084\/jem.20051038","article-title":"Characterization of marginal zone B cell precursors","volume":"202","author":"B Srivastava","year":"2005","journal-title":"J Exp Med"},{"issue":"6","key":"pone.0161468.ref021","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.4049\/jimmunol.171.6.2783","article-title":"Notch2 haploinsufficiency results in diminished B1 B cells and a severe reduction in marginal zone B cells","volume":"171","author":"CM Witt","year":"2003","journal-title":"J Immunol"},{"issue":"6","key":"pone.0161468.ref022","doi-asserted-by":"crossref","first-page":"e0131961","DOI":"10.1371\/journal.pone.0131961","article-title":"ARID3B Directly Regulates Ovarian Cancer Promoting Genes","volume":"10","author":"A Bobbs","year":"2015","journal-title":"PLoS One"},{"issue":"18","key":"pone.0161468.ref023","doi-asserted-by":"crossref","first-page":"8355","DOI":"10.18632\/oncotarget.2247","article-title":"ARID3B increases ovarian tumor burden and is associated with a cancer stem cell gene signature","volume":"5","author":"L Roy","year":"2014","journal-title":"Oncotarget"},{"issue":"8","key":"pone.0161468.ref024","doi-asserted-by":"crossref","first-page":"e105557","DOI":"10.1371\/journal.pone.0105557","article-title":"The corepressor Tle4 is a novel regulator of murine hematopoiesis and bone development","volume":"9","author":"JC Wheat","year":"2014","journal-title":"PLoS One"},{"issue":"1\u20132","key":"pone.0161468.ref025","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.molimm.2011.09.008","article-title":"The transcription factor Bright plays a role in marginal zone B lymphocyte development and autoantibody production","volume":"49","author":"AL Oldham","year":"2011","journal-title":"Mol Immunol"},{"issue":"3","key":"pone.0161468.ref026","doi-asserted-by":"crossref","first-page":"940","DOI":"10.4049\/jimmunol.1401941","article-title":"Differential expression of the transcription factor ARID3a in lupus patient hematopoietic progenitor cells","volume":"194","author":"ML Ratliff","year":"2015","journal-title":"J Immunol"},{"key":"pone.0161468.ref027","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1476-4598-6-23","article-title":"Bright\/ARID3A contributes to chromatin accessibility of the immunoglobulin heavy chain enhancer","volume":"6","author":"D Lin","year":"2007","journal-title":"Mol Cancer"},{"issue":"1","key":"pone.0161468.ref028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1476-9433-3-1","article-title":"NF-kB inhibitor blocks B cell development at two checkpoints","volume":"3","author":"B Feng","year":"2004","journal-title":"Med Immunol"},{"issue":"2","key":"pone.0161468.ref029","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0092-8674(95)90415-8","article-title":"Targeted disruption of the p50 subunit of NF-kappa B leads to multifocal defects in immune responses","volume":"80","author":"WC Sha","year":"1995","journal-title":"Cell"},{"issue":"12","key":"pone.0161468.ref030","doi-asserted-by":"crossref","first-page":"6825","DOI":"10.4049\/jimmunol.165.12.6825","article-title":"Genetic analyses of NFKB1 and OCA-B function: defects in B cells, serum IgM level, and antibody responses in Nfkb1-\/-Oca-b-\/- mice","volume":"165","author":"U Kim","year":"2000","journal-title":"J Immunol"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0309551","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"record-id":"55971"},{"DOI":"10.1371\/journal.pone.0309551","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000}}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0161468","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T15:17:09Z","timestamp":1724858229000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0161468"}},"subtitle":[],"editor":[{"given":"Kevin D","family":"Bunting","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,8,18]]},"references-count":30,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2016,8,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0161468","relation":{"retraction":[{"id-type":"doi","id":"10.1371\/journal.pone.0309551","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,18]]}}}