{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:18:52Z","timestamp":1790137132479,"version":"4.0.1"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T00:00:00Z","timestamp":1473120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/CIRG\/1367\/2013"],"award-info":[{"award-number":["NMRC\/CIRG\/1367\/2013"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/CIRG\/1406\/2014"],"award-info":[{"award-number":["NMRC\/CIRG\/1406\/2014"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institute of Bioengineering and Nanotechnology"},{"name":"Tessa Therapeutics Pte Ltd."}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0161820","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T17:40:56Z","timestamp":1473183656000},"page":"e0161820","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":56,"title":["Co-Expansion of Cytokine-Induced Killer Cells and V\u03b39V\u03b42 T Cells for CAR T-Cell Therapy"],"prefix":"10.1371","volume":"11","author":[{"given":"Shou-Hui","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhendong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wee-Kiat","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixia","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4029-3533","authenticated-orcid":true,"given":"Timothy Weixin","family":"Kwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Hu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,9,6]]},"reference":[{"issue":"6230","key":"ref1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1126\/science.aaa4967","article-title":"Adoptive cell transfer as personalized immunotherapy for human cancer","volume":"348","author":"SA Rosenberg","year":"2015","journal-title":"Science"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/j.ejcts.2009.11.051","article-title":"A phase I study of adoptive immunotherapy for recurrent non-small-cell lung cancer patients with autologous gammadelta T cells","volume":"37","author":"J Nakajima","year":"2010","journal-title":"European journal of cardio-thoracic surgery: official journal of the European Association for Cardio-thoracic Surgery"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/hon.886","article-title":"Immunotherapy with cytokine induced killer cells in solid and hematopoietic tumours: a pilot clinical trial","volume":"27","author":"P Olioso","year":"2009","journal-title":"Hematological oncology"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1517\/14712598.2012.675323","article-title":"Cytokine-induced killer (CIK) cells as feasible and effective adoptive immunotherapy for the treatment of solid tumors","volume":"12","author":"G Mesiano","year":"2012","journal-title":"Expert opinion on biological therapy"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/1479-5876-8-129","article-title":"Thymoglobulin, interferon-gamma and interleukin-2 efficiently expand cytokine-induced killer (CIK) cells in clinical-grade cultures","volume":"8","author":"G Bonanno","year":"2010","journal-title":"Journal of translational medicine"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1046\/j.1365-2141.2002.03247.x","article-title":"Generation of cytokine-induced killer cells from leukaemic samples with in vitro cytotoxicity against autologous and allogeneic leukaemic blasts","volume":"116","author":"YC Linn","year":"2002","journal-title":"British journal of haematology"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"e78980","DOI":"10.1371\/journal.pone.0078980","article-title":"Sunitinib indirectly enhanced anti-tumor cytotoxicity of cytokine-induced killer cells and CD3(+)CD56(+) subset through the co-culturing dendritic cells","volume":"8","author":"A Wongkajornsilp","year":"2013","journal-title":"PloS one"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1007\/s00432-012-1179-1","article-title":"The effects of cytokine-induced killer cells for the treatment of patients with solid tumors: a clinical retrospective study","volume":"138","author":"J Zhang","year":"2012","journal-title":"Journal of cancer research and clinical oncology"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1038\/cmi.2015.28","article-title":"The promise of gammadelta T cells and the gammadelta T cell receptor for cancer immunotherapy","volume":"12","author":"M Legut","year":"2015","journal-title":"Cellular & molecular immunology"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/cmi.2012.39","article-title":"What lessons can be learned from gamma delta T cell-based cancer immunotherapy trials?","volume":"10","author":"JJ Fournie","year":"2013","journal-title":"Cellular & molecular immunology"},{"issue":"8","key":"ref11","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1007\/s00262-011-1021-7","article-title":"Phase I\/II study of adoptive transfer of gamma delta T cells in combination with zoledronic acid and IL-2 to patients with advanced renal cell carcinoma","volume":"60","author":"H Kobayashi","year":"2011","journal-title":"Cancer immunology, immunotherapy: CII"},{"issue":"5","key":"ref12","first-page":"755","article-title":"Targeting gamma delta T cells for cancer immunotherapy: bench to bedside","volume":"138","author":"D Gogoi","year":"2013","journal-title":"The Indian journal of medical research"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/cam4.196","article-title":"Intraperitoneal injection of in vitro expanded Vgamma9Vdelta2 T cells together with zoledronate for the treatment of malignant ascites due to gastric cancer","volume":"3","author":"I Wada","year":"2014","journal-title":"Cancer medicine"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/nri3384","article-title":"Six-of-the-best: unique contributions of gamma delta T cells to immunology","volume":"13","author":"P Vantourout","year":"2013","journal-title":"Nature reviews Immunology"},{"key":"ref15","first-page":"292436","article-title":"Corrigendum to "Molecular Characterization of a Fully Human Chimeric T-Cell Antigen Receptor for Tumor-Associated Antigen EpCAM"","author":"N Shirasu","year":"2015","journal-title":"Biomed Res Int"},{"issue":"10","key":"ref16","doi-asserted-by":"crossref","first-page":"4285","DOI":"10.1073\/pnas.89.10.4285","article-title":"Humanization of an anti-p185HER2 antibody for human cancer therapy","volume":"89","author":"P Carter","year":"1992","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"13","key":"ref17","doi-asserted-by":"crossref","first-page":"4232","DOI":"10.1158\/1078-0432.CCR-07-4912","article-title":"Isopentenyl pyrophosphate-activated CD56+ {gamma}{delta} T lymphocytes display potent antitumor activity toward human squamous cell carcinoma","volume":"14","author":"AA Alexander","year":"2008","journal-title":"Clinical cancer research: an official journal of the American Association for Cancer Research"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/intimm\/dxh337","article-title":"Activation of Vgamma9Vdelta2 T cells by non-peptidic antigens induces the inhibition of subgenomic HCV replication","volume":"18","author":"C Agrati","year":"2006","journal-title":"International immunology"},{"key":"ref19","article-title":"Expansion of human peripheral blood gammadelta T cells using zoledronate","volume":"55","author":"M Kondo","year":"2011","journal-title":"Journal of visualized experiments: JoVE"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1365-2141.2004.05077.x","article-title":"Human gammadelta T cells as mediators of chimaeric-receptor redirected anti-tumour immunity","volume":"126","author":"M Rischer","year":"2004","journal-title":"British journal of haematology"},{"issue":"11","key":"ref21","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.humimm.2006.08.292","article-title":"Activation-induced expression of CD56 by T cells is associated with a reprogramming of cytolytic activity and cytokine secretion profile in vitro","volume":"67","author":"J Kelly-Rogers","year":"2006","journal-title":"Human immunology"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.3324\/haematol.2010.026310","article-title":"Cytokine-induced killer cells for cell therapy of acute myeloid leukemia: improvement of their immune activity by expression of CD33-specific chimeric receptors","volume":"95","author":"V Marin","year":"2010","journal-title":"Haematologica"},{"issue":"12","key":"ref23","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1038\/mt.2013.192","article-title":"Arming cytokine-induced killer cells with chimeric antigen receptors: CD28 outperforms combined CD28-OX40 "super-stimulation"","volume":"21","author":"AA Hombach","year":"2013","journal-title":"Molecular therapy: the journal of the American Society of Gene Therapy"},{"issue":"12","key":"ref24","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1007\/s002620000148","article-title":"CD58\/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells","volume":"49","author":"RD Lopez","year":"2001","journal-title":"Cancer immunology, immunotherapy: CII"},{"issue":"12","key":"ref25","doi-asserted-by":"crossref","first-page":"4245","DOI":"10.4049\/jimmunol.140.12.4245","article-title":"Anti-proliferative effect of IFN-gamma in immune regulation. I. IFN-gamma inhibits the proliferation of Th2 but not Th1 murine helper T lymphocyte clones","volume":"140","author":"TF Gajewski","year":"1988","journal-title":"Journal of immunology"},{"issue":"22","key":"ref26","doi-asserted-by":"crossref","first-page":"5708","DOI":"10.1158\/1078-0432.CCR-13-3451","article-title":"Activating and propagating polyclonal gamma delta T cells with broad specificity for malignancies","volume":"20","author":"DC Deniger","year":"2014","journal-title":"Clin Cancer Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"636","DOI":"10.3389\/fimmu.2014.00636","article-title":"Clinical applications of gamma delta T cells with multivalent immunity","volume":"5","author":"DC Deniger","year":"2014","journal-title":"Front Immunol"},{"issue":"8","key":"ref28","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1158\/0008-5472.CAN-07-5600","article-title":"Redirecting specificity of T-cell populations for CD19 using the Sleeping Beauty system","volume":"68","author":"H Singh","year":"2008","journal-title":"Cancer research"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/1479-5876-8-104","article-title":"Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: differing impact on CD8 T cell phenotype and responsiveness to restimulation","volume":"8","author":"Y Li","year":"2010","journal-title":"Journal of translational medicine"},{"issue":"1\u20132","key":"ref30","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jim.2011.01.002","article-title":"Development of a genetically-modified novel T-cell receptor for adoptive cell transfer against renal cell carcinoma","volume":"366","author":"QJ Wang","year":"2011","journal-title":"Journal of immunological methods"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/imr.12129","article-title":"Human cell-based artificial antigen-presenting cells for cancer immunotherapy","volume":"257","author":"MO Butler","year":"2014","journal-title":"Immunological reviews"},{"issue":"5","key":"ref32","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1038\/mt.sj.6300134","article-title":"Engineering artificial antigen-presenting cells to express a diverse array of co-stimulatory molecules","volume":"15","author":"MM Suhoski","year":"2007","journal-title":"Molecular therapy: the journal of the American Society of Gene Therapy"},{"issue":"8","key":"ref33","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/nm730","article-title":"Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion","volume":"8","author":"H Dong","year":"2002","journal-title":"Nature medicine"},{"issue":"7","key":"ref34","doi-asserted-by":"crossref","first-page":"4910","DOI":"10.4049\/jimmunol.179.7.4910","article-title":"4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy","volume":"179","author":"H Zhang","year":"2007","journal-title":"Journal of immunology"},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.clim.2010.07.009","article-title":"4-1BB-mediated expansion affords superior detection of in vivo primed effector memory CD8+ T cells from melanoma sentinel lymph nodes","volume":"137","author":"BJ Sluijter","year":"2010","journal-title":"Clinical immunology"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/imr.12131","article-title":"Design and development of therapies using chimeric antigen receptor-expressing T cells","volume":"257","author":"G Dotti","year":"2014","journal-title":"Immunological reviews"},{"issue":"10","key":"ref37","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1007\/s00262-014-1572-5","article-title":"A GMP-compliant protocol to expand and transfect cancer patient T cells with mRNA encoding a tumor-specific chimeric antigen receptor","volume":"63","author":"C Krug","year":"2014","journal-title":"Cancer immunology, immunotherapy: CII"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0161820","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T23:56:06Z","timestamp":1692489366000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0161820"}},"subtitle":[],"editor":[{"given":"Francesco","family":"Dieli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,9,6]]},"references-count":37,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2016,9,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0161820","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,6]]}}}