{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:32:21Z","timestamp":1789428741582,"version":"build-2803163510"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2016,9,7]],"date-time":"2016-09-07T00:00:00Z","timestamp":1473206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572503"],"award-info":[{"award-number":["31572503"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangsu province agricultural science and technology independent innovation funds","award":["cx(16)1028"],"award-info":[{"award-number":["cx(16)1028"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0162274","type":"journal-article","created":{"date-parts":[[2016,9,7]],"date-time":"2016-09-07T17:37:24Z","timestamp":1473269844000},"page":"e0162274","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Development of a Sandwich ELISA for EHEC O157:H7 Intimin \u03b31"],"prefix":"10.1371","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0647-2266","authenticated-orcid":true,"given":"Xuehan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bicheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangming","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kongwang","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,9,7]]},"reference":[{"issue":"6\u20137","key":"ref1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.ijmm.2005.06.009","article-title":"Enterohaemorrhagic <italic>Escherichia coli<\/italic> in human medicine","volume":"295","author":"H Karch","year":"2005","journal-title":"International Journal of Medical Microbiology"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1089\/fpd.2010.0673","article-title":"<italic>Escherichia coli<\/italic> O157:H7: Animal Reservoir and Sources of Human Infection","volume":"8","author":"WA Ferens","year":"2011","journal-title":"Foodborne Pathogens and Disease"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ijfoodmicro.2006.10.046","article-title":"Serotypes, virulence genes, and intimin types of Shiga toxin-producing <italic>Escherichia coli<\/italic> (STEC) and enteropathogenic <italic>E<\/italic>. <italic>coli<\/italic> (EPEC) isolated from calves in S\u00e3o Paulo, Brazil","volume":"115","author":"L Aidar-Ugrinovich","year":"2007","journal-title":"International Journal of Food Microbiology"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1099\/jmm.0.46518-0","article-title":"Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (\u03bcB and \u03beR\/\u03b22B)","volume":"55","author":"M Blanco","year":"2006","journal-title":"Journal of Medical Microbiology"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1128\/JCM.42.2.645-651.2004","article-title":"Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin)-Producing <italic>Escherichia coli<\/italic> Isolates from Cattle in Spain and Identification of a New Intimin Variant Gene (<italic>eae-\u03be<\/italic>)","volume":"42","author":"M Blanco","year":"2004","journal-title":"Journal of Clinical Microbiology"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1365-2672.2003.02170.x","article-title":"Strategies for the detection of Escherichia coli O157:H7 in foods","volume":"96","author":"AK Deisingh","year":"2004","journal-title":"Journal of Applied Microbiology"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"3809","DOI":"10.1128\/AEM.58.12.3809-3815.1992","article-title":"Rapid and sensitive method for detection of Shiga-like toxin-producing <italic>Escherichia coli<\/italic> in ground beef using the polymerase chain reaction","volume":"58","author":"VP Gannon","year":"1992","journal-title":"Applied and Environmental Microbiology"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1128\/JCM.33.1.248-250.1995","article-title":"Simultaneous identification of strains of <italic>Escherichia coli<\/italic> serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR","volume":"33","author":"TA Cebula","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4315\/0362-028X-67.1.27","article-title":"Simultaneous Detection of <italic>Escherichia coli<\/italic> O157:H7, <italic>Salmonella<\/italic>, and <italic>Shigella<\/italic> in Apple Cider and Produce by a Multiplex PCR","volume":"67","author":"Y Li","year":"2004","journal-title":"Journal of Food Protection"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1128\/JCM.00608-09","article-title":"Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic <italic>Escherichia coli<\/italic>","volume":"47","author":"F Barletta","year":"2009","journal-title":"Journal of Clinical Microbiology"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/BF00193628","article-title":"Differentiation in virulence patterns of Escherichia coli possessing eae genes","volume":"183","author":"H Schmidt","year":"1994","journal-title":"Med Microbiol Immunol"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1006\/mcpr.1997.0132","article-title":"Detection and characterization of the fimA gene of Escherichia coli O157:H7","volume":"11","author":"B Li","year":"1997","journal-title":"Molecular and Cellular Probes"},{"issue":"15","key":"ref13","doi-asserted-by":"crossref","first-page":"5297","DOI":"10.1128\/AEM.00794-12","article-title":"Real-Time PCR Methodology for Selective Detection of Viable <italic>Escherichia coli<\/italic> O157:H7 Cells by Targeting Z3276 as a Genetic Marker","volume":"78","author":"B Li","year":"2012","journal-title":"Applied and Environmental Microbiology"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1128\/JCM.35.5.1066-1070.1997","article-title":"Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM","volume":"35","author":"PI Fields","year":"1997","journal-title":"Journal of Clinical Microbiology"},{"issue":"6","key":"ref15","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1128\/JCM.36.6.1801-1804.1998","article-title":"A PCR Specific for <italic>Escherichia coli<\/italic> O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide","volume":"36","author":"PM Desmarchelier","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1046\/j.1365-2672.2001.01281.x","article-title":"Development of a monoclonal sandwich ELISA for the detection of animal and human <italic>Escherichia coli<\/italic> O157 strains","volume":"90","author":"P Kerr","year":"2001","journal-title":"Journal of Applied Microbiology"},{"issue":"22","key":"ref17","doi-asserted-by":"crossref","first-page":"3923","DOI":"10.1016\/j.vaccine.2011.02.007","article-title":"Subcutaneous and intranasal immunization with Stx2B\u2013Tir\u2013Stx1B\u2013Zot reduces colonization and shedding of <italic>Escherichia coli<\/italic> O157:H7 in mice","volume":"29","author":"X-h Zhang","year":"2011","journal-title":"Vaccine"},{"issue":"5517","key":"ref18","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/256495a0","article-title":"Continuous cultures of fused cells secreting antibody of predefined specificity","volume":"256","author":"G Kohler","year":"1975","journal-title":"Nature"},{"issue":"5","key":"ref19","doi-asserted-by":"crossref","first-page":"e96366","DOI":"10.1371\/journal.pone.0096366","article-title":"Development and evaluation of a DAS-ELISA for rapid detection of Tembusu virus using monoclonal antibodies against the envelope protein","volume":"9","author":"H Chen","year":"2014","journal-title":"PloS one"},{"issue":"6","key":"ref20","first-page":"58","article-title":"Duplex PCR Procedure for the Detection of EHEC O157: H7","volume":"26","author":"L Li","year":"2011","journal-title":"Acta Agri Boreali-Sincia"},{"issue":"8","key":"ref21","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1128\/IAI.58.8.2438-2445.1990","article-title":"Mouse model for colonization and disease caused by enterohemorrhagic <italic>Escherichia coli<\/italic> O157:H7","volume":"58","author":"EA Wadolkowski","year":"1990","journal-title":"Infection and Immunity"},{"issue":"5","key":"ref22","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1128\/JCM.31.5.1256-1261.1993","article-title":"Determination of the optimal cutoff value for a serological assay: an example using the Johne&apos;s Absorbed EIA","volume":"31","author":"SE Ridge","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0378-1135(02)00125-6","article-title":"Development of a capture ELISA for the detection of antibodies to enteropathogenic <italic>Escherichia coli<\/italic> (EPEC) in rabbit flocks using intimin-specific monoclonal antibodies","volume":"88","author":"DGF Vandekerchove","year":"2002","journal-title":"Veterinary Microbiology"},{"issue":"14","key":"ref24","article-title":"Prokaryotic expression of Enterohemorrhagic Escherichia coli O157:H7 eae gene and preliminary establishment of an indirect ELISA","volume":"53","author":"L Luo","year":"2014","journal-title":"Hubei Agri Sci"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"3318","DOI":"10.3168\/jds.2011-5093","article-title":"Vaccination of pregnant cows with EspA, EspB, gamma-intimin, and Shiga toxin 2 proteins from <italic>Escherichia coli<\/italic> O157:H7 induces high levels of specific colostral antibodies that are transferred to newborn calves","volume":"95","author":"BC Rabinovitz","year":"2012","journal-title":"Journal of dairy science"},{"issue":"1","key":"ref26","first-page":"46","article-title":"Immunological characterization of <italic>Escherichia coli<\/italic> O157:H7 intimin gamma1","volume":"9","author":"WG Son","year":"2002","journal-title":"Clinical and diagnostic laboratory immunology"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"e91632","DOI":"10.1371\/journal.pone.0091632","article-title":"Immunization with H7-HCP-tir-intimin significantly reduces colonization and shedding of <italic>Escherichia coli<\/italic> O157:H7 in goats","volume":"9","author":"X Zhang","year":"2014","journal-title":"PloS one"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1757-4749-6-14","article-title":"A novel enzyme-linked immunosorbent assay for detection of <italic>Escherichia coli<\/italic> O157:H7 using immunomagnetic and beacon gold nanoparticles","volume":"6","author":"Z Shen","year":"2014","journal-title":"Gut Pathogens"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"e8700","DOI":"10.1371\/journal.pone.0008700","article-title":"Derivation of <italic>Escherichia coli<\/italic> O157:H7 from its O55:H7 precursor","volume":"5","author":"Z Zhou","year":"2010","journal-title":"PloS one"},{"issue":"10","key":"ref30","doi-asserted-by":"crossref","first-page":"e76368","DOI":"10.1371\/journal.pone.0076368","article-title":"A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by Escherichia coli in human and environmental samples","volume":"8","author":"X He","year":"2013","journal-title":"PloS one"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4142\/jvs.2005.6.1.41","article-title":"Development of a sandwich ELISA for the detection of <italic>Listeria<\/italic> spp. using specific flagella antibodies","volume":"6","author":"SH Kim","year":"2005","journal-title":"Journal of veterinary science"},{"issue":"4","key":"ref32","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF01570719","article-title":"Effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi","volume":"25","author":"V Roger","year":"1992","journal-title":"Current microbiology"},{"issue":"10","key":"ref33","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.4315\/0362-028X.JFP-14-596","article-title":"Risk of <italic>Escherichia coli<\/italic> O157:H7, Non-O157 Shiga Toxin-Producing <italic>Escherichia coli<\/italic>, and Campylobacter spp. in Food Animals and Their Products in Qatar","volume":"78","author":"HO Mohammed","year":"2015","journal-title":"J Food Prot"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0162274","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T20:57:01Z","timestamp":1601067421000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0162274"}},"subtitle":[],"editor":[{"given":"George-John","family":"Nychas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,9,7]]},"references-count":33,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2016,9,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0162274","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,7]]}}}