{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T03:56:51Z","timestamp":1794023811866,"version":"4.8.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T00:00:00Z","timestamp":1474416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence National pour la Recherhce","award":["ANR-05-MIIM-031"],"award-info":[{"award-number":["ANR-05-MIIM-031"]}]},{"name":"Intiative d'Exellence","award":["DYNAMO, ANR-11-LABX-0011"],"award-info":[{"award-number":["DYNAMO, ANR-11-LABX-0011"]}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["E024519"],"award-info":[{"award-number":["E024519"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0162434","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T19:28:07Z","timestamp":1474486087000},"page":"e0162434","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Phosphocarrier Protein HPr Contributes to Meningococcal Survival during Infection"],"prefix":"10.1371","volume":"11","author":[{"given":"Ana","family":"Antunes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Derkaoui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aude","family":"Terrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Denizon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ala-Eddine","family":"Deghmane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Deutscher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Delany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhamed-Kheir","family":"Taha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,9,21]]},"reference":[{"issue":"Pt 9","key":"ref1","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1099\/jmm.0.45529-0","article-title":"<italic>Neisseria meningitidis<\/italic>: an overview of the carriage state","volume":"53","author":"SP Yazdankhah","year":"2004","journal-title":"J Med Microbiol"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1574-6976.2006.00052.x","article-title":"Lessons from meningococcal carriage studies","volume":"31","author":"DA Caugant","year":"2007","journal-title":"FEMS Microbiol Rev"},{"issue":"Suppl 2","key":"ref3","doi-asserted-by":"crossref","first-page":"B64","DOI":"10.1016\/j.vaccine.2009.04.061","article-title":"Meningococcal carriage and disease\u2014population biology and evolution","volume":"27","author":"DA Caugant","year":"2009","journal-title":"Vaccine"},{"issue":"18","key":"ref4","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1056\/NEJM200105033441807","article-title":"Meningococcal disease","volume":"344","author":"NE Rosenstein","year":"2001","journal-title":"N Engl J Med"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"98","DOI":"10.4161\/viru.26515","article-title":"Meningococcal disease and the complement system","volume":"5","author":"LA Lewis","year":"2014","journal-title":"Virulence"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1101\/gr.664303","article-title":"Large-scale analysis of the meningococcus genome by gene disruption: resistance to complement-mediated lysis","volume":"13","author":"MC Geoffroy","year":"2003","journal-title":"Genome Res"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"5609","DOI":"10.1128\/IAI.00781-07","article-title":"Transgenic mice expressing human transferrin as a model for meningococcal infection","volume":"75","author":"ML Zarantonelli","year":"2007","journal-title":"Infect Immun"},{"issue":"7","key":"ref8","doi-asserted-by":"crossref","first-page":"4489","DOI":"10.4049\/jimmunol.178.7.4489","article-title":"Factor H binding and function in sialylated pathogenic <italic>Neisseriae<\/italic> is influenced by gonococcal, but not meningococcal, porin","volume":"178","author":"G Madico","year":"2007","journal-title":"J Immunol"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1128\/IAI.01071-08","article-title":"Factor H-binding protein is important for meningococcal survival in human whole blood and serum and in the presence of the antimicrobial peptide LL-37","volume":"77","author":"KL Seib","year":"2009","journal-title":"Infect Immun"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","first-page":"5063","DOI":"10.4049\/jimmunol.1103748","article-title":"The relative roles of factor H binding protein, neisserial surface protein A, and lipooligosaccharide sialylation in regulation of the alternative pathway of complement on meningococci","volume":"188","author":"LA Lewis","year":"2012","journal-title":"J Immunol"},{"issue":"7","key":"ref11","doi-asserted-by":"crossref","first-page":"e1001027","DOI":"10.1371\/journal.ppat.1001027","article-title":"The meningococcal vaccine candidate neisserial surface protein A (NspA) binds to factor H and enhances meningococcal resistance to complement","volume":"6","author":"LA Lewis","year":"2010","journal-title":"PLoS Pathog"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"2574","DOI":"10.1128\/IAI.01517-14","article-title":"Inhibition of the alternative pathway of nonhuman infant complement by porin B2 contributes to virulence of <italic>Neisseria meningitidis<\/italic> in the infant rat model","volume":"82","author":"LA Lewis","year":"2014","journal-title":"Infect Immun"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1073\/pnas.1321556111","article-title":"<italic>Neisseria meningitidis<\/italic> NalP cleaves human complement C3, facilitating degradation of C3b and survival in human serum","volume":"111","author":"E Del Tordello","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/JB.101.1.127-132.1970","article-title":"Catabolic activities of <italic>Neisseria meningitidis<\/italic>: utilization of glutamate","volume":"101","author":"LP Mallavia","year":"1970","journal-title":"J Bacteriol"},{"issue":"Pt 6","key":"ref15","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1099\/00221287-147-6-1473","article-title":"An NMR and enzyme study of the carbon metabolism of <italic>Neisseria meningitidis<\/italic>","volume":"147","author":"MP Leighton","year":"2001","journal-title":"Microbiology"},{"issue":"9","key":"ref16","doi-asserted-by":"crossref","first-page":"4190","DOI":"10.1128\/IAI.00117-07","article-title":"Effect of host lactate on gonococci and meningococci: new concepts on the role of metabolites in pathogenicity","volume":"75","author":"H Smith","year":"2007","journal-title":"Infect Immun"},{"key":"ref17","article-title":"The phosphocarrier protein HPr of <italic>Neisseria meningitidis<\/italic> interacts with the transcription regulator CrgA and its deletion affects capsule production, cell adhesion and virulence","author":"M Derkaoui","year":"2016","journal-title":"Mol Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3389\/fcimb.2014.00114","article-title":"Metabolism and virulence in <italic>Neisseria meningitidis<\/italic>","volume":"4","author":"C Schoen","year":"2014","journal-title":"Front Cell Infect Microbiol"},{"issue":"11","key":"ref19","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1038\/81380","article-title":"Functional genomics of <italic>Neisseria meningitidis<\/italic> pathogenesis","volume":"6","author":"YH Sun","year":"2000","journal-title":"Nat Med"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1128\/MMBR.00001-14","article-title":"The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions","volume":"78","author":"J Deutscher","year":"2014","journal-title":"Microbiol Mol Biol Rev"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1128\/MMBR.00024-06","article-title":"How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria","volume":"70","author":"J Deutscher","year":"2006","journal-title":"Microbiol Mol Biol Rev"},{"issue":"7","key":"ref22","doi-asserted-by":"crossref","first-page":"e22210","DOI":"10.1371\/journal.pone.0022210","article-title":"Experimental meningococcal sepsis in congenic transgenic mice expressing human transferrin","volume":"6","author":"M Szatanik","year":"2011","journal-title":"PLoS One"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/jb.85.6.1274-1279.1963","article-title":"<italic>Neisseria gonorrhoeae<\/italic>. I. Virulence Genetically Linked to Clonal Variation","volume":"85","author":"DS Kellogg Jr.","year":"1963","journal-title":"J Bacteriol"},{"issue":"5459","key":"ref24","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1126\/science.287.5459.1809","article-title":"Complete genome sequence of <italic>Neisseria meningitidis<\/italic> serogroup B strain MC58","volume":"287","author":"H Tettelin","year":"2000","journal-title":"Science"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1186\/1471-2334-14-276","article-title":"Lipocalin 2 in cerebrospinal fluid as a marker of acute bacterial meningitis","volume":"14","author":"T Guiddir","year":"2014","journal-title":"BMC Infect Dis"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0022-2836(83)80284-8","article-title":"Studies on transformation of Escherichia coli with plasmids","volume":"166","author":"D Hanahan","year":"1983","journal-title":"J Mol Biol"},{"key":"ref27","article-title":"Experiments in Molecular Genetics","author":"JH Miller","year":"1972"},{"key":"ref28","article-title":"Molecular cloning: A laboratory Manual","author":"J Sambrook","year":"1989"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.vaccine.2012.10.072","article-title":"Functional impacts of the diversity of the meningococcal factor H binding protein","volume":"31","author":"E Hong","year":"2012","journal-title":"Vaccine"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0882-4010(88)90045-9","article-title":"<italic>Neisseria meningitidis<\/italic> group B serosubtyping using monoclonal antibodies in whole-cell ELISA","volume":"4","author":"H Abdillahi","year":"1988","journal-title":"Microb Pathog"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/JB.154.1.269-277.1983","article-title":"Morphological heterogeneity among <italic>Salmonella<\/italic> lipopolysaccharide chemotypes in silver-stained polyacrylamide gels","volume":"154","author":"PJ Hitchcock","year":"1983","journal-title":"J Bacteriol"},{"issue":"4","key":"ref32","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1128\/IAI.01216-08","article-title":"The RNA chaperone Hfq is involved in stress response and virulence in <italic>Neisseria meningitidis<\/italic> and is a pleiotropic regulator of protein expression","volume":"77","author":"L Fantappie","year":"2009","journal-title":"Infect Immun"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.mib.2014.11.006","article-title":"<italic>Neisseria meningitidis<\/italic>: pathogenesis and immunity","volume":"23","author":"M Pizza","year":"2015","journal-title":"Curr Opin Microbiol"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0378-1097(03)00558-5","article-title":"Insertional inactivation of the <italic>lpxA<\/italic> gene involved in the biosynthesis of lipid A in <italic>Neisseria meningitidis<\/italic> resulted in <italic>lpxA\/lpxA<\/italic>::<italic>aph-3&apos;<\/italic> heterodiploids","volume":"226","author":"ML Zarantonelli","year":"2003","journal-title":"FEMS Microbiol Lett"},{"issue":"6675","key":"ref36","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/33046","article-title":"Meningitis bacterium is viable without endotoxin","volume":"392","author":"L Steeghs","year":"1998","journal-title":"Nature"},{"key":"ref37","first-page":"155","article-title":"Bacterial moonlighting proteins and bacterial virulence","volume":"358","author":"B Henderson","year":"2013","journal-title":"Curr Top Microbiol Immunol"},{"issue":"11","key":"ref38","doi-asserted-by":"crossref","first-page":"e81818","DOI":"10.1371\/journal.pone.0081818","article-title":"Interaction of Leptospira elongation factor Tu with plasminogen and complement factor H: a metabolic leptospiral protein with moonlighting activities","volume":"8","author":"DG Wolff","year":"2013","journal-title":"PLoS One"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/j.1365-2958.2010.07098.x","article-title":"The moonlighting protein fructose-1, 6-bisphosphate aldolase of <italic>Neisseria meningitidis<\/italic>: surface localization and role in host cell adhesion","volume":"76","author":"SA Tunio","year":"2010","journal-title":"Mol Microbiol"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.tim.2007.03.005","article-title":"Interactions between <italic>Neisseria meningitidis<\/italic> and the complement system","volume":"15","author":"MC Schneider","year":"2007","journal-title":"Trends Microbiol"},{"issue":"5","key":"ref41","doi-asserted-by":"crossref","first-page":"e01625","DOI":"10.1128\/mBio.01625-14","article-title":"Human factor H (FH) impairs protective meningococcal anti-FHbp antibody responses and the antibodies enhance FH binding","volume":"5","author":"I Costa","year":"2014","journal-title":"MBio"},{"key":"ref42","article-title":"Evolutionary events associated with an outbreak of meningococcal disease in men who have sex with men","author":"M-K Taha","year":"2016","journal-title":"PLoS One"},{"issue":"2","key":"ref43","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1128\/IAI.05604-11","article-title":"Enhanced bacteremia in human factor H transgenic rats infected by <italic>Neisseria meningitidis<\/italic>","volume":"80","author":"DM Vu","year":"2012","journal-title":"Infect Immun"},{"issue":"38","key":"ref44","doi-asserted-by":"crossref","first-page":"4192","DOI":"10.1016\/j.vaccine.2013.06.009","article-title":"fH-dependent complement evasion by disease-causing meningococcal strains with absent fHbp genes or frameshift mutations","volume":"31","author":"S Giuntini","year":"2013","journal-title":"Vaccine"},{"issue":"11","key":"ref45","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1111\/j.1462-5822.2006.00792.x","article-title":"How do microbes evade neutrophil killing?","volume":"8","author":"CF Urban","year":"2006","journal-title":"Cell Microbiol"},{"issue":"11","key":"ref46","doi-asserted-by":"crossref","first-page":"2987","DOI":"10.1128\/JB.184.11.2987-2993.2002","article-title":"Nitric oxide metabolism in <italic>Neisseria meningitidis<\/italic>","volume":"184","author":"MF Anjum","year":"2002","journal-title":"J Bacteriol"},{"issue":"1","key":"ref47","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1096\/fj.08-128330","article-title":"Bacterial nitric oxide detoxification prevents host cell S-nitrosothiol formation: a novel mechanism of bacterial pathogenesis","volume":"24","author":"JR Laver","year":"2010","journal-title":"FASEB J"},{"issue":"3","key":"ref48","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1128\/JB.01368-06","article-title":"Regulation of denitrification genes in <italic>Neisseria meningitidis<\/italic> by nitric oxide and the repressor NsrR","volume":"189","author":"JD Rock","year":"2007","journal-title":"J Bacteriol"},{"issue":"4295","key":"ref49","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1126\/science.860134","article-title":"Evolution and tinkering","volume":"196","author":"F Jacob","year":"1977","journal-title":"Science"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"e1002027","DOI":"10.1371\/journal.ppat.1002027","article-title":"Transcriptome analysis of <italic>Neisseria meningitidis<\/italic> in human whole blood and mutagenesis studies identify virulence factors involved in blood survival","volume":"7","author":"H Echenique-Rivera","year":"2011","journal-title":"PLoS Pathog"},{"issue":"8","key":"ref51","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1073\/pnas.0915162107","article-title":"<italic>Neisseria meningitidis<\/italic> GNA2132, a heparin-binding protein that induces protective immunity in humans","volume":"107","author":"D Serruto","year":"2010","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0162434","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T23:12:42Z","timestamp":1657321962000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0162434"}},"subtitle":[],"editor":[{"given":"Thomas","family":"Rudel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,9,21]]},"references-count":51,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2016,9,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0162434","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,21]]}}}