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World Malaria Report 2015. Geneva: WHO, 2016."},{"key":"ref2","unstructured":"Group MVF. Malaria Vaccine Technology Roadmap. 2013."},{"key":"ref3","unstructured":"Group MVF. Malaria Vaccine Technology Roadmap. 2006."},{"key":"ref4","unstructured":"First malaria vaccine receives positive scientific opinion from EMA [Internet]. 2015. Mosquirix to be used for vaccination of young children, together with established antimalarial interventions; 24 July 2015. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.ema.europa.eu\/ema\/index.jsp?curl=pages\/news_and_events\/news\/2015\/07\/news_detail_002376.jsp&mid=WC0b01ac058004d5c1\" xlink:type=\"simple\">http:\/\/www.ema.europa.eu\/ema\/index.jsp?curl=pages\/news_and_events\/news\/2015\/07\/news_detail_002376.jsp&amp;mid=WC0b01ac058004d5c1<\/ext-link>"},{"issue":"12","key":"ref5","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1136\/archdischild-2013-304173","article-title":"Malaria vaccines: past, present and future","volume":"98","author":"L von Seidlein","year":"2013","journal-title":"Arch Dis Child"},{"issue":"7","key":"ref6","doi-asserted-by":"crossref","first-page":"e1001685","DOI":"10.1371\/journal.pmed.1001685","article-title":"Efficacy and safety of the RTS,S\/AS01 malaria vaccine during 18 months after vaccination: a phase 3 randomized, controlled trial in children and young infants at 11 African 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