{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:34:47Z","timestamp":1792802087074,"version":"4.5.0"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d\u2019Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2014 SGR-132"],"award-info":[{"award-number":["2014 SGR-132"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CONICYT-Chile","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}]},{"DOI":"10.13039\/501100007136","name":"Secretar\u00eda de Estado de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["Ram\u00f3n y Cajal (RYC-2010-06210)"],"award-info":[{"award-number":["Ram\u00f3n y Cajal (RYC-2010-06210)"]}],"id":[{"id":"10.13039\/501100007136","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003741","name":"ICREA","doi-asserted-by":"crossref","award":["ICREA academia award"],"award-info":[{"award-number":["ICREA academia award"]}],"id":[{"id":"10.13039\/501100003741","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007136","name":"Secretar\u00eda de Estado de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AGL2012-33877"],"award-info":[{"award-number":["AGL2012-33877"]}],"id":[{"id":"10.13039\/501100007136","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","doi-asserted-by":"publisher","award":["RTA2012-00028-C02-02"],"award-info":[{"award-number":["RTA2012-00028-C02-02"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d\u2019Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2014 SGR-345"],"award-info":[{"award-number":["2014 SGR-345"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (ES)","id":[{"id":"https:\/\/ror.org\/01gm5f004","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0164073","type":"journal-article","created":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T13:30:33Z","timestamp":1475847033000},"page":"e0164073","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms"],"prefix":"10.1371","volume":"11","author":[{"given":"D\u00e9bora","family":"Torrealba","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquin","family":"Seras-Franzoso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Mamat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Wilke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Villaverde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nerea","family":"Roher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7498-4864","authenticated-orcid":true,"given":"Elena","family":"Garcia-Fruit\u00f3s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,10,7]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1586\/14760584.4.1.89","article-title":"Vaccines for fish in aquaculture","volume":"4","author":"I Sommerset","year":"2005","journal-title":"Expert Review of Vaccines"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.fsi.2013.05.029","article-title":"Status and future perspectives of vaccines for industrialised fin-fish farming","volume":"35","author":"BE Brudeseth","year":"2013","journal-title":"Fish Shellfish Immunol"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10126-010-9279-x","article-title":"Immunological control of fish diseases","volume":"12","author":"B Magnadottir","year":"2010","journal-title":"Mar Biotechnol (NY)"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1002\/jps.21523","article-title":"Vaccine adjuvants: current challenges and future approaches","volume":"98","author":"JH Wilson-Welder","year":"2009","journal-title":"J Pharm Sci"},{"issue":"6","key":"ref5","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1016\/j.fsi.2013.02.029","article-title":"Adjuvants and immunostimulants in fish vaccines: Current knowledge and future perspectives","volume":"35","author":"C Tafalla","year":"2013","journal-title":"Fish & Shellfish Immunology"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.fsi.2007.03.004","article-title":"Bacterial lipopolysaccharides induce genes involved in the innate immune response in embryos of the zebrafish (Danio rerio)","volume":"23","author":"J Watzke","year":"2007","journal-title":"Fish & Shellfish Immunology"},{"issue":"1\u20132","key":"ref7","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetimm.2006.06.011","article-title":"Adjuvant and immunostimulatory effects of beta-glucan administration in combination with lipopolysaccharide enhances survival and some immune parameters in carp challenged with Aeromonas hydrophila","volume":"114","author":"V Selvaraj","year":"2006","journal-title":"Vet Immunol Immunopathol"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.fsi.2012.11.025","article-title":"In vivo effects of Escherichia coli lipopolysaccharide on regulation of immune response and protein expression in striped catfish (Pangasianodon hypophthalmus)","volume":"34","author":"BT Hang","year":"2013","journal-title":"Fish Shellfish Immunol"},{"issue":"7\u20138","key":"ref9","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1016\/j.molimm.2010.02.009","article-title":"Peptidoglycan, not endotoxin, is the key mediator of cytokine gene expression induced in rainbow trout macrophages by crude LPS","volume":"47","author":"SA MacKenzie","year":"2010","journal-title":"Molecular Immunology"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cbd.2005.07.003","article-title":"Conservation of Toll-Like Receptor Signaling Pathways in Teleost Fish","volume":"1","author":"MK Purcell","year":"2006","journal-title":"Comp Biochem Physiol Part D Genomics Proteomics"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.fsi.2015.12.037","article-title":"Yersinia ruckeri lipopolysaccharide is necessary and sufficient for eliciting a protective immune response in rainbow trout (Oncorhynchus mykiss, Walbaum)","volume":"49","author":"TJ Welch","year":"2016","journal-title":"Fish Shellfish Immunol"},{"issue":"31","key":"ref12","doi-asserted-by":"crossref","first-page":"3955","DOI":"10.1016\/j.vaccine.2014.05.010","article-title":"Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS\/dsRNA-loaded nanoliposomes","volume":"32","author":"A Ruyra","year":"2014","journal-title":"Vaccine"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.tibtech.2011.09.003","article-title":"Bacterial inclusion bodies: making gold from waste","volume":"30","author":"E Garcia-Fruitos","year":"2012","journal-title":"Trends in Biotechnology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/1475-2859-9-80","article-title":"Inclusion bodies: a new concept","volume":"9","author":"E Garcia-Fruitos","year":"2010","journal-title":"Microbial Cell Factories"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/1475-2859-9-71","article-title":"Isolation of cell-free bacterial inclusion bodies","volume":"9","author":"E Rodriguez-Carmona","year":"2010","journal-title":"Microbial Cell Factories"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/S0076-6879(99)09005-9","article-title":"Isolating inclusion bodies from bacteria","volume":"309","author":"G Georgiou","year":"1999","journal-title":"Methods Enzymol"},{"issue":"42","key":"ref17","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1002\/adma.200900283","article-title":"Surface Cell Growth Engineering Assisted by a Novel Bacterial Nanomaterial","volume":"21","author":"E Garcia-Fruitos","year":"2009","journal-title":"Advanced Materials"},{"issue":"19","key":"ref18","doi-asserted-by":"crossref","first-page":"5165","DOI":"10.1128\/JB.180.19.5165-5172.1998","article-title":"Roles of the Escherichia coli small heat shock proteins IbpA and IbpB in thermal stress management: Comparison with ClpA, ClpB, and HtpG in vivo","volume":"180","author":"JG Thomas","year":"1998","journal-title":"Journal of Bacteriology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s12934-015-0241-5","article-title":"Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins","volume":"14","author":"U Mamat","year":"2015","journal-title":"Microb Cell Fact"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1177\/1753425909348232","article-title":"Endotoxin recognition in fish results in inflammatory cytokine secretion not gene expression","volume":"17","author":"N Roher","year":"2011","journal-title":"Innate Immun"},{"issue":"14","key":"ref21","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1039\/C5SM02930A","article-title":"Cellular uptake and intracellular fate of protein releasing bacterial amyloids in mammalian cells","volume":"12","author":"J Seras-Franzoso","year":"2016","journal-title":"Soft Matter"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0167-7012(03)00193-3","article-title":"Rapid screening method for quantitation of bacterial cell lipids from whole cells","volume":"55","author":"J Izard","year":"2003","journal-title":"J Microbiol Methods"},{"issue":"13","key":"ref24","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1002\/adma.201104330","article-title":"Functional Inclusion Bodies Produced in Bacteria as Naturally Occurring Nanopills for Advanced Cell Therapies","volume":"24","author":"E Vazquez","year":"2012","journal-title":"Advanced Materials"},{"issue":"10","key":"ref25","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.2217\/nnm.12.188","article-title":"A nanostructured bacterial bioscaffold for the sustained bottom-up delivery of protein drugs","volume":"8","author":"J Seras-Franzoso","year":"2013","journal-title":"Nanomedicine (Lond)"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1939-1676.2003.tb02448.x","article-title":"Adjuvants in veterinary vaccines: modes of action and adverse effects","volume":"17","author":"AR Spickler","year":"2003","journal-title":"J Vet Intern Med"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jmb.2007.09.004","article-title":"Divergent genetic control of protein solubility and conformational quality in Escherichia coli","volume":"374","author":"E Garcia-Fruitos","year":"2007","journal-title":"Journal of Molecular Biology"},{"issue":"20","key":"ref28","doi-asserted-by":"crossref","DOI":"10.1088\/0957-4484\/21\/20\/205101","article-title":"Tunable geometry of bacterial inclusion bodies as substrate materials for tissue engineering","volume":"21","author":"E Garcia-Fruitos","year":"2010","journal-title":"Nanotechnology"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"6134","DOI":"10.1016\/j.actbio.2012.11.033","article-title":"Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies","volume":"9","author":"O Cano-Garrido","year":"2013","journal-title":"Acta Biomater"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0164073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T09:39:47Z","timestamp":1601113187000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0164073"}},"subtitle":[],"editor":[{"given":"Uwe","family":"Fischer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,10,7]]},"references-count":29,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2016,10,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0164073","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,7]]}}}