{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T20:44:16Z","timestamp":1780519456557,"version":"3.54.1"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T00:00:00Z","timestamp":1475712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chinese 12th Five-Year Plan","award":["No. 2012BAI32B03"],"award-info":[{"award-number":["No. 2012BAI32B03"]}]},{"name":"Central Special Talent Construction Funds of China","award":["Qiancaijiao 2015-134"],"award-info":[{"award-number":["Qiancaijiao 2015-134"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0164116","type":"journal-article","created":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T17:38:12Z","timestamp":1475775492000},"page":"e0164116","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Reference Ranges and Association of Age and Lifestyle Characteristics with Testosterone, Sex Hormone Binding Globulin, and Luteinizing Hormone among 1166 Western Chinese Men"],"prefix":"10.1371","volume":"11","author":[{"given":"Xubo","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honggang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengliang","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7293-1395","authenticated-orcid":true,"given":"Ellen M.","family":"Wells","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,10,6]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.mcna.2011.03.001","article-title":"Late-onset hypogonadism","volume":"95","author":"N Bassil","year":"2011","journal-title":"Medical Clinics of North America"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"b352","DOI":"10.1136\/bmj.b352","article-title":"Late onset hypogonadism","volume":"338","author":"TH Jones","year":"2009","journal-title":"British Medical Journal"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1530\/EJE-08-0601","article-title":"Investigation, treatment and monitoring of late-onset hypogonadism in males: ISA, ISSAM, EAU, EAA and ASA recommendations","volume":"159","author":"C Wang","year":"2008","journal-title":"European Journal of Endocrinology"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.1442-2042.2008.02203.x","article-title":"Late-onset hypogonadism (LOH) and androgens: validity of the measurement of free testosterone levels in the diagnostic criteria in Japan","volume":"16","author":"T Iwamoto","year":"2009","journal-title":"International Journal of Urology"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1210\/jc.2011-2513","article-title":"Characteristics of androgen deficiency in late-onset hypogonadism: results from the European Male Aging Study (EMAS)","volume":"97","author":"A Tajar","year":"2012","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1210\/jc.2013-2052","article-title":"Late-onset hypogonadism and mortality in aging men","volume":"99","author":"SR Pye","year":"2014","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1743-6109.2012.02783.x","article-title":"Testosterone deficiency in men: systematic review and standard operating procedures for diagnosis and treatment","volume":"10","author":"J Buvat","year":"2013","journal-title":"Journal of Sexual Medicine"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1210\/jcem.86.2.7219","article-title":"Longitudinal effects of aging on serum total and free testosterone levels in healthy men. Baltimore Longitudinal Study of Aging","volume":"86","author":"SM Harman","year":"2001","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"12","key":"ref9","doi-asserted-by":"crossref","first-page":"5920","DOI":"10.1210\/jc.2003-031719","article-title":"Prevalence and incidence of androgen deficiency in middle-aged and older men: estimates from the Massachusetts Male Aging Study","volume":"89","author":"AB Araujo","year":"2004","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1056\/NEJMoa0911101","article-title":"Identification of late-onset hypogonadism in middle-aged and elderly men","volume":"363","author":"FC Wu","year":"2010","journal-title":"New England Journal of Medicine"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1530\/EJE-12-0890","article-title":"Age-associated changes in hypothalamic-pituitary-testicular function in middle-aged and older men are modified by weight change and lifestyle factors: longitudinal results from the European Male Ageing Study","volume":"168","author":"EM Camacho","year":"2013","journal-title":"European Journal of Endocrinology"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3109\/13685538.2012.666585","article-title":"Correlation between sex hormone levels and obesity in the elderly male","volume":"15","author":"J Cao","year":"2012","journal-title":"Aging Male"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1111\/cen.12768","article-title":"The association of obesity with sex hormone-binding globulin is stronger than the association with ageing\u2013implications for the interpretation of total testosterone measurements","volume":"83","author":"LA Cooper","year":"2015","journal-title":"Clinical Endocrinology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s12958-015-0107-z","article-title":"Dynamic alteration of serum testosterone with aging: a cross-sectional study from Shanghai, China","volume":"13","author":"Z Liu","year":"2015","journal-title":"Reproductive Biology and Endocrinology"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1530\/eje.0.1490145","article-title":"The associations of age, lifestyle factors and chronic disease with testosterone in men: the Tromso Study","volume":"149","author":"J Svartberg","year":"2003","journal-title":"European Journal of Endocrinology"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/13685530802389628","article-title":"ISA, ISSAM, EAU, EAA and ASA recommendations: investigation, treatment and monitoring of late-onset hypogonadism in males","volume":"12","author":"C Wang","year":"2009","journal-title":"Aging Male"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/13685530500163416","article-title":"ISA, ISSAM and EAU recommendations for the investigation, treatment and monitoring of late-onset hypogonadism in males: scientific background and rationale","volume":"8","author":"B Lunenfeld","year":"2005","journal-title":"Aging Male"},{"issue":"9","key":"ref18","doi-asserted-by":"crossref","first-page":"3599","DOI":"10.1210\/jc.2007-0862","article-title":"Age-related changes in serum testosterone and sex hormone binding globulin in Australian men: longitudinal analyses of two geographically separate regional cohorts","volume":"92","author":"PY Liu","year":"2007","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1210\/jcem.87.2.8201","article-title":"Age trends in the level of serum testosterone and other hormones in middle-aged men: longitudinal results from the Massachusetts male aging study","volume":"87","author":"HA Feldman","year":"2002","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"10","key":"ref20","doi-asserted-by":"crossref","first-page":"e109346","DOI":"10.1371\/journal.pone.0109346","article-title":"A validated age-related normative model for male total testosterone shows increasing variance but no decline after age 40 years","volume":"9","author":"TW Kelsey","year":"2014","journal-title":"PLoS One"},{"issue":"3\u20134","key":"ref21","first-page":"203","article-title":"Decline of serum levels of free testosterone in aging healthy Chinese men","volume":"8","author":"JY Li","year":"2005","journal-title":"Aging Male"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/j.1447-0594.2009.00552.x","article-title":"Age-related changes in plasma androgen levels and their association with cardiovascular risk factors in male Japanese office workers","volume":"10","author":"S Fukai","year":"2010","journal-title":"Geriatrics and Gerontology International"},{"issue":"11","key":"ref23","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1210\/jc.2007-1245","article-title":"Prevalence of symptomatic androgen deficiency in men","volume":"92","author":"AB Araujo","year":"2007","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"8","key":"ref24","doi-asserted-by":"crossref","first-page":"2430","DOI":"10.1210\/jc.2010-3012","article-title":"Reference ranges for testosterone in men generated using liquid chromatography tandem mass spectrometry in a community-based sample of healthy nonobese young men in the Framingham Heart Study and applied to three geographically distinct cohorts","volume":"96","author":"S Bhasin","year":"2011","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.eururo.2005.04.027","article-title":"Investigation, treatment and monitoring of late-onset hypogonadism in males. ISA, ISSAM, and EAU recommendations","volume":"48","author":"E Nieschlag","year":"2005","journal-title":"European Urology"},{"issue":"11","key":"ref26","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1055\/s-0034-1395569","article-title":"Total Testosterone and Calculated Estimates for Free and Bioavailable Testosterone: Influence of Age and Body Mass Index and Establishment of Sex-Specific Reference Ranges","volume":"47","author":"T Deutschbein","year":"2015","journal-title":"Hormone and Metabolic Research"},{"issue":"6","key":"ref27","doi-asserted-by":"crossref","first-page":"610","DOI":"10.2164\/jandrol.108.005561","article-title":"Reference ranges for serum dehydroepiandrosterone sulfate and testosterone in adult men","volume":"29","author":"N Friedrich","year":"2008","journal-title":"Journal of Andrology"},{"issue":"7","key":"ref28","doi-asserted-by":"crossref","first-page":"2647","DOI":"10.1210\/jc.2015-4106","article-title":"Low Free Testosterone is Associated with Hypogonadal Signs and Symptoms in Men with Normal Total Testosterone","volume":"101","author":"L Antonio","year":"2016","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/jsm.12393","article-title":"Prevalence of symptoms and associated comorbidities of testosterone deficiency syndrome in the Korean general population","volume":"11","author":"DG Moon","year":"2014","journal-title":"Journal of Sexual Medicine"},{"issue":"3\u20134","key":"ref30","first-page":"281","article-title":"Reference intervals for serum sex hormones in Han Chinese adult men from the Fangchenggang Area Male Health and Examination Survey","volume":"58","author":"X Qin","year":"2012","journal-title":"Clinical Laboratory"},{"issue":"5","key":"ref31","doi-asserted-by":"crossref","first-page":"747","DOI":"10.4103\/1008-682X.160883","article-title":"Identification of late-onset hypogonadism in middle-aged and elderly men from a community of China","volume":"18","author":"ZY Liu","year":"2016","journal-title":"Asian Journal of Andrology"},{"issue":"8","key":"ref32","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1093\/humrep\/deu118","article-title":"Alcohol and male reproductive health: a cross-sectional study of 8344 healthy men from Europe and the USA","volume":"29","author":"TK Jensen","year":"2014","journal-title":"Human Reproduction"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"403","DOI":"10.2147\/DMSO.S34220","article-title":"Predicting cardiometabolic risk: waist-to-height ratio or BMI. A meta-analysis","volume":"6","author":"SC Savva","year":"2013","journal-title":"Diabetes, Metabolic Syndrome and Obesity"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1046\/j.1365-2265.2003.01772.x","article-title":"Diurnal rhythms of serum total, free and bioavailable testosterone and of SHBG in middle-aged men compared with those in young men","volume":"58","author":"MJ Diver","year":"2003","journal-title":"Clinical Endocrinology"},{"issue":"10","key":"ref35","doi-asserted-by":"crossref","first-page":"3666","DOI":"10.1210\/jcem.84.10.6079","article-title":"A critical evaluation of simple methods for the estimation of free testosterone in serum","volume":"84","author":"A Vermeulen","year":"1999","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3109\/13685538.2013.835800","article-title":"Free testosterone by direct and calculated measurement versus equilibrium dialysis in a clinical population","volume":"16","author":"R Kacker","year":"2013","journal-title":"Aging Male"},{"issue":"5","key":"ref37","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1442-2042.2008.02010.x","article-title":"Clinical practice manual for late-onset hypogonadism syndrome","volume":"15","author":"M Namiki","year":"2008","journal-title":"International Journal of Urology"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.mce.2008.07.016","article-title":"Leydig cell aging and the mechanisms of reduced testosterone synthesis","volume":"299","author":"AS Midzak","year":"2009","journal-title":"Molecular and Cellular Endocrinology"},{"issue":"7056","key":"ref39","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1136\/bmj.313.7056.559d","article-title":"Ratio of waist circumference to height is strong predictor of intra-abdominal fat","volume":"313","author":"M Ashwell","year":"1996","journal-title":"British Medical Journal"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"10","DOI":"10.7326\/0003-4819-138-1-200301070-00006","article-title":"Systolic blood pressure, diastolic blood pressure, and pulse pressure as predictors of risk for congestive heart failure in the Framingham Heart Study","volume":"138","author":"AW Haider","year":"2003","journal-title":"Annals of Internal Medicine"},{"issue":"10","key":"ref41","first-page":"3025","article-title":"Amplified nocturnal luteinizing hormone (LH) secretory burst frequency with selective attenuation of pulsatile (but not basal) testosterone secretion in healthy aged men: possible Leydig cell desensitization to endogenous LH signaling\u2014a clinical research center study","volume":"80","author":"T Mulligan","year":"1995","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"5","key":"ref42","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/0022-4731(75)90069-2","article-title":"Rhythms in the secretion of gonadotropins and gonadal steroids","volume":"6","author":"H Baker","year":"1975","journal-title":"Journal of Steroid Biochemistry"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1210\/jcem-71-6-1616","article-title":"Twenty-Four-Hour Rhythms in Plasma Concentrations of Adenohypophyseal Hormones Are Generated by Distinct Amplitude and\/or Frequency Modulation of Underlying Pituitary Secretory Bursts","volume":"71","author":"JD Veldhuis","year":"1990","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"3","key":"ref44","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1097\/MED.0b013e328353732f","article-title":"Evolving utility of sex hormone-binding globulin measurements in clinical medicine","volume":"19","author":"GL Hammond","year":"2012","journal-title":"Current Opinion in Endocrinology, Diabetes and Obesity"},{"issue":"4","key":"ref45","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1016\/j.juro.2011.11.095","article-title":"Performance of total testosterone measurement to predict free testosterone for the biochemical evaluation of male hypogonadism","volume":"187","author":"BD Anawalt","year":"2012","journal-title":"Journal of Urology"},{"issue":"7","key":"ref46","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1210\/jc.2007-1972","article-title":"Hypothalamic-pituitary-testicular axis disruptions in older men are differentially linked to age and modifiable risk factors: the European Male Aging Study","volume":"93","author":"FC Wu","year":"2008","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"8","key":"ref47","doi-asserted-by":"crossref","first-page":"3289","DOI":"10.1210\/jc.2012-3842","article-title":"Longitudinal changes in testosterone over five years in community-dwelling men","volume":"98","author":"Z Shi","year":"2013","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"4","key":"ref48","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.beem.2013.05.002","article-title":"Diagnosis and treatment of late-onset hypogonadism: systematic review and meta-analysis of TRT outcomes","volume":"27","author":"G Corona","year":"2013","journal-title":"Best Practice and Research in Clinical Endocrinology and Metabolism"},{"issue":"7","key":"ref49","first-page":"557","article-title":"Hypogonadism and metabolic syndrome","volume":"34","author":"G Corona","year":"2011","journal-title":"Journal of Endocrinological Investigation"},{"issue":"2","key":"ref50","doi-asserted-by":"crossref","first-page":"131","DOI":"10.2174\/157339912799424573","article-title":"Testosterone as potential effective therapy in treatment of obesity in men with testosterone deficiency: a review","volume":"8","author":"F Saad","year":"2012","journal-title":"Current Diabetes Reviews"},{"issue":"4","key":"ref51","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1210\/jc.2005-1830","article-title":"Testosterone and estradiol among older men","volume":"91","author":"E Orwoll","year":"2006","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"6","key":"ref52","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1210\/jc.2009-2354","article-title":"Testosterone therapy in men with androgen deficiency syndromes: an Endocrine Society clinical practice guideline","volume":"95","author":"S Bhasin","year":"2010","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"issue":"2","key":"ref53","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.urology.2015.05.007","article-title":"Association of Free Testosterone With Hypogonadal Symptoms in Men With Near-normal Total Testosterone Levels","volume":"86","author":"R Ramasamy","year":"2015","journal-title":"Urology"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0168029","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2016,12,22]],"date-time":"2016-12-22T00:00:00Z","timestamp":1482364800000}}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0164116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T15:56:06Z","timestamp":1589039766000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0164116"}},"subtitle":[],"editor":[{"given":"Petter","family":"Bjornstad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,10,6]]},"references-count":53,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2016,10,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0164116","relation":{"correction":[{"id-type":"doi","id":"10.1371\/journal.pone.0168029","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,6]]}}}