{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:27:50Z","timestamp":1790774870299,"version":"4.1.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005971","name":"Deutsche Herzstiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005971","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0164308","type":"journal-article","created":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T13:32:55Z","timestamp":1478007175000},"page":"e0164308","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["MMP-2 Isoforms in Aortic Tissue and Serum of Patients with Ascending Aortic Aneurysms and Aortic Root Aneurysms"],"prefix":"10.1371","volume":"11","author":[{"given":"Anke","family":"Tscheuschler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Meffert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedhelm","family":"Beyersdorf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Heilmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadja","family":"Kocher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xenia","family":"Uffelmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Discher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Siepe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian A.","family":"Kari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,11,1]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/j.jacc.2013.12.028","article-title":"How does the ascending aorta geometry change when it dissects?","volume":"63","author":"B Rylski","year":"2014","journal-title":"J Am Coll Cardiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.jss.2006.09.020","article-title":"Proteinase systems and thoracic aortic aneurysm progression","volume":"139","author":"JR Barbour","year":"2007","journal-title":"J Surg Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1161\/01.RES.0000070112.80711.3D","article-title":"Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases Structure, Function, and Biochemistry","volume":"92","author":"R Visse","year":"2003","journal-title":"Circ Res"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"S186","DOI":"10.1161\/CIRCULATIONAHA.112.000363","article-title":"Reproducible Porcine Model of Thoracic Aortic Aneurysm","volume":"128","author":"SR Eckhouse","year":"2013","journal-title":"Circulation"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.athoracsur.2011.09.057","article-title":"Aortic Dilatation With Bicuspid Aortic Valves: Cusp Fusion Correlates to Matrix Metalloproteinases and Inhibitors","volume":"93","author":"JS Ikonomidis","year":"2012","journal-title":"Ann Thorac Surg"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0741-5214(95)70110-9","article-title":"Increased turnover of collagen in abdominal aortic aneurysms, demonstrated by measuring the concentration of the aminoterminal propeptide of type III procollagen in peripheral and aortal blood samples","volume":"22","author":"J Satta","year":"1995","journal-title":"J Vasc Surg"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1161\/01.CIR.82.3.973","article-title":"Elastase-induced experimental aneurysms in rats","volume":"82","author":"S Anidjar","year":"1990","journal-title":"Circulation"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1172\/JCI0215334","article-title":"Matrix metalloproteinases 2 and 9 work in concert to produce aortic aneurysms","volume":"110","author":"GM Longo","year":"2002","journal-title":"J Clin Invest"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1042\/cs0810233","article-title":"Metalloproteinases in degenerative aortic disease","volume":"81","author":"N Vine","year":"1991","journal-title":"Clin Sci"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"4136","DOI":"10.1038\/srep04136","article-title":"Type IV collagen \u03b11-chain noncollagenous domain blocks MMP-2 activation both in-vitro and in-vivo","volume":"4","author":"YA Sudhakar","year":"2014","journal-title":"Sci Rep"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"10337","DOI":"10.1074\/jbc.M512009200","article-title":"Individual Timp Deficiencies Differentially Impact Pro-MMP-2 Activation","volume":"281","author":"JL English","year":"2006","journal-title":"J Biol Chem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.matbio.2015.03.005","article-title":"The role of TIMPs in regulation of extracellular matrix proteolysis","volume":"44\u201346","author":"V Arpino","year":"2015","journal-title":"Matrix Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"5331","DOI":"10.1074\/jbc.270.10.5331","article-title":"Mechanism of cell surface activation of 72-kDa type IV collagenase. Isolation of the activated form of the membrane metalloprotease","volume":"270","author":"AY Strongin","year":"1995","journal-title":"J Biol Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1146\/annurev.cellbio.17.1.463","article-title":"How Matrix Metalloproteinases Regulate Cell Behavior","volume":"17","author":"MD Sternlicht","year":"2001","journal-title":"Annu Rev Cell Dev Biol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1111\/j.1440-1681.2010.05441.x","article-title":"Matrix metalloproteinase-2 regulates the expression of tissue inhibitor of matrix metalloproteinase-2","volume":"37","author":"K Kimura","year":"2010","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"ref16","article-title":"Divergent Role of Matrix Metalloproteinase 2 in Pathogenesis of Thoracic Aortic Aneurysm","author":"M Shen","year":"2015","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e80","DOI":"10.1161\/01.RES.0000077044.60138.7C","article-title":"Mechanical Stretch Enhances mRNA Expression and Proenzyme Release of Matrix Metalloproteinase-2 (MMP-2) via NAD(P)H Oxidase\u2013Derived Reactive Oxygen Species","volume":"92","author":"K Grote","year":"2003","journal-title":"Circ Res"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.jacc.2015.06.1310","article-title":"Valve-Related Hemodynamics Mediate Human Bicuspid Aortopathy: Insights From Wall Shear Stress Mapping","volume":"66","author":"DG Guzzardi","year":"2015","journal-title":"J Am Coll Cardiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"E120","DOI":"10.1161\/01.ATV.20.12.e120","article-title":"Role of matrix metalloproteinases in blood flow-induced arterial enlargement: interaction with NO","volume":"20","author":"F Tronc","year":"2000","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.jtcvs.2005.02.052","article-title":"Mechanical deterioration underlies malignant behavior of aneurysmal human ascending aorta","volume":"130","author":"G Koullias","year":"2005","journal-title":"J Thorac Cardiovasc Surg"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1093\/icvts\/ivu446","article-title":"Four-dimensional magnetic resonance imaging-derived ascending aortic flow eccentricity and flow compression are linked to aneurysm morphology\u2020","volume":"20","author":"FA Kari","year":"2015","journal-title":"Interact Cardiovasc Thorac Surg"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-1-61779-854-2_8","article-title":"Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography","volume":"878","author":"M Toth","year":"2012","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"H Towbin","year":"1979","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/j.jtcvs.2012.04.008","article-title":"Elevated messenger RNA expression and plasma protein levels of osteopontin and matrix metalloproteinase types 2 and 9 in patients with ascending aortic aneurysms","volume":"145","author":"T Huusko","year":"2013","journal-title":"J Thorac Cardiovasc Surg"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.pcad.2013.04.002","article-title":"Pathophysiology of thoracic aortic aneurysm (TAA): is it not one uniform aorta? Role of embryologic origin","volume":"56","author":"JM Ruddy","year":"2013","journal-title":"Prog Cardiovasc Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1038\/nrcardio.2009.191","article-title":"Cellular and molecular mechanisms of thoracic aortic aneurysms","volume":"6","author":"I El-Hamamsy","year":"2009","journal-title":"Nat Rev Cardiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1161\/CIRCRESAHA.114.304936","article-title":"Role of Mechanotransduction in Vascular Biology Focus on Thoracic Aortic Aneurysms and Dissections","volume":"116","author":"JD Humphrey","year":"2015","journal-title":"Circ Res"},{"key":"ref28","first-page":"916","article-title":"The genetic basis for aortic aneurysmal disease","volume":"100","author":"A Saratzis","year":"2014","journal-title":"Heart Br Card Soc"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1056\/NEJMra1207059","article-title":"Aortic Dilatation in Patients with Bicuspid Aortic Valve","volume":"370","author":"S Verma","year":"2014","journal-title":"N Engl J Med"},{"key":"ref30","article-title":"Bicuspid aortic valve hemodynamics induces abnormal medial remodeling in the convexity of porcine ascending aortas","author":"SK Atkins","year":"2014","journal-title":"Biomech Model Mechanobiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0002-9149(77)80004-0","article-title":"Histologic changes in the normal aging aorta: Implications for dissecting aortic aneurysm","volume":"39","author":"TJM Schlatmann","year":"1977","journal-title":"Am J Cardiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1016\/j.jtcvs.2012.12.027","article-title":"Plasma biomarkers for distinguishing etiological subtypes of thoracic aortic aneurysm disease","volume":"145","author":"JS Ikonomidis","year":"2013","journal-title":"J Thorac Cardiovasc Surg"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1161\/CIRCGENETICS.111.960419","article-title":"Selective microRNA Suppression in Human Thoracic Aneurysms: Relationship of miR-29a to Aortic Size and Proteolytic Induction","volume":"4","author":"JA Jones","year":"2011","journal-title":"Circ Cardiovasc Genet"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jss.2004.06.007","article-title":"Matrix metalloproteinases in ascending aortic aneurysms: bicuspid versus trileaflet aortic valves","volume":"123","author":"SA LeMaire","year":"2005","journal-title":"J Surg Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1038\/sj.bjc.6601725","article-title":"Zymographic detection and clinical correlations of MMP-2 and MMP-9 in breast cancer sera","volume":"90","author":"GL Rocca","year":"2004","journal-title":"Br J Cancer"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"10337","DOI":"10.1074\/jbc.M512009200","article-title":"Individual Timp Deficiencies Differentially Impact Pro-MMP-2 Activation","volume":"281","author":"JL English","year":"2006","journal-title":"J Biol Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0022-4804(03)00193-8","article-title":"A murine model of thoracic aortic aneurysms","volume":"115","author":"JS Ikonomidis","year":"2003","journal-title":"J Surg Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1136\/ard.2005.039032","article-title":"Assessment of the clinical significance of gelatinase activity in patients with juvenile idiopathic arthritis using quantitative protein substrate zymography","volume":"65","author":"NJ Peake","year":"2006","journal-title":"Ann Rheum Dis"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s00395-014-0419-8","article-title":"Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta","volume":"109","author":"I Kopaliani","year":"2014","journal-title":"Basic Res Cardiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0164308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:50:11Z","timestamp":1588967411000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0164308"}},"subtitle":[],"editor":[{"given":"Rudolf","family":"Kirchmair","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,11,1]]},"references-count":39,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2016,11,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0164308","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,1]]}}}