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Lyme disease data and statistics. 2012; Available: http:\/\/www.cdc.gov\/lyme\/stats\/index.html<\/ext-link>. Accessed June 2012."},{"key":"ref12","unstructured":"Centers for Disease Control and Prevention. Anaplasmosis, statistics and epidemiology. 2013. Available: http:\/\/www.cdc.gov\/anaplasmosis\/stats\/<\/ext-link>. Accessed October 2013"},{"key":"ref13","unstructured":"Centers for Disease Control and Prevention. Lyme disease data and statistics. 2012. Available: http:\/\/www.cdc.gov\/lyme\/stats\/index.html<\/ext-link>. Accessed June 2012."},{"key":"ref14","unstructured":"Centers for Disease Control and Prevention. Press Release, CDC provides estimate of Americans diagnosed with Lyme disease each year. 2013. Available: http:\/\/www.cdc.gov\/media\/releases\/2013\/p0819-lyme-disease.html<\/ext-link>. Accessed Novemeber 2013."},{"key":"ref15","unstructured":"Wisconsin Department of Health Services. Anaplasmosis and Ehrlichiosis. http:\/\/www.dhs.wisconsin.gov\/communicable\/Tickborne\/AandE\/Index.htm<\/ext-link>. 2010. Available: http:\/\/www.dhs.wisconsin.gov\/communicable\/Tickborne\/AandE\/Index.htm<\/ext-link>. Accessed Novemebr 2013."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/S1473-3099(15)00464-8","article-title":"Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study","volume":"16","author":"B Pritt","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"ref17","first-page":"455","article-title":"Infectious diseases of wild mammals","author":"W Davidson","year":"2001"},{"key":"ref18","first-page":"435","article-title":"Infectious diseases of wild mammals","author":"R Brown","year":"2001"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"355","DOI":"10.4269\/ajtmh.1985.34.355","article-title":"Mice as reservoirs of the Lyme disease spirochete","volume":"34","author":"J Levine","year":"1985","journal-title":"Am J Trop Med Hyg"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"92","DOI":"10.4269\/ajtmh.1987.36.92","article-title":"Reservoir competence of white-footed mice of Lyme disease spirochetes","volume":"36","author":"J Donahue","year":"1987","journal-title":"Am J Trop Med Hyg"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1093\/oxfordjournals.aje.a115306","article-title":"Comparing the relative potential of rodents as reservoirs of the Lyme disease spirochete (Borrelia burgdorferi<\/italic>)","volume":"130","author":"T Mather","year":"1989","journal-title":"Am J Epidemiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6209","DOI":"10.1073\/pnas.93.12.6209","article-title":"Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle","volume":"93","author":"S Telford","year":"1996","journal-title":"Proc Natl Acad Sci"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"522","DOI":"10.7589\/0090-3558-24.3.522","article-title":"Susceptibility of the gray wolf (Canis lupus<\/italic>) to infection with the Lyme disease agent, Borrelia burgdorferi<\/italic>","volume":"24","author":"J Kazmierczak","year":"1988","journal-title":"J Wildl Dis"},{"key":"ref24","unstructured":"Beheler-Amass K, Wydeven A, Thiel R. Wolf health and monitoring and mortality factors, appendix F. 1999. Available: http:\/\/www.timberwolfinformation.org\/info\/wolfmanplan\/final\/appendix\/appendix_f.htm<\/ext-link>. Accessed October 2013."},{"key":"ref25","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/CMR.4.3.286","article-title":"The tribe Ehrlichieae and ehrlichial diseases","volume":"4","author":"Y Rikihisa","year":"1991","journal-title":"Clin Microbiol Rev"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"937","DOI":"10.2460\/ajvr.1975.36.07.937","article-title":"Transmission of Ehrlichia canis<\/italic> to dogs by ticks (Rhipicephalus sanguineus<\/italic>)","volume":"36","author":"M Groves","year":"1975","journal-title":"Am J Vet Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-5-245","article-title":"Key factors influencing canine heartworm, Dirofilaria immitis<\/italic>, in the United States","volume":"5","author":"H Brown","year":"2012","journal-title":"Parasites and Vectors"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.vetpar.2005.04.007","article-title":"Public health aspects of dirofilariasis in the United States","volume":"133","author":"J Theis","year":"2005","journal-title":"Vet Parasitol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1378\/chest.112.3.729","article-title":"Human pulmonary dirofilariasis: analysis of 24 cases from S\u00e3o Paulo, Brazil","volume":"112","author":"JRM de Campos","year":"1997","journal-title":"Chest"},{"key":"ref30","first-page":"87","article-title":"Recovery of gray wolves in the Great Lakes Region of the United States","author":"A Wydeven","year":"2009"},{"key":"ref31","unstructured":"Wydeven A, Wiedenhoeft J, Schultz R, Bruner J, Thiel R, Boles S, et al. Status of the timber wolf in Wisconsin: performance report 1 July 2010 through 30 June 2011. Wisconsin Endangered Resources Report # 141. Madison, Wisconsin; 2011."},{"key":"ref32","unstructured":"Wisconsin Wolf Management Plan. Wisconsin Department of Natural Resources, PUBL-ER-099 99. Madison, Wisconsin, USA; 1999."},{"key":"ref33","unstructured":"Department of the Interior. Endangered and Threatened wildlife and plants; reinstatement of final rules for the gray wolf in Wyoming and the Western Great Lakes in compliance with court orders. Fedruary 20, 2015. Federal Register Notice 50 CFR Part 17, Fish and Wildlife Service, Int. 2015."},{"key":"ref34","first-page":"351","article-title":"Pendulum swings in wolf management led to conflict, illegal kills, and a legislated wolf hunt","volume":"8","author":"O ER","year":"2014","journal-title":"Conserv Lett"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.3201\/eid0803.010166","article-title":"Predicting the risk of Lyme disease: habitat suitability for Ixodes scapularis<\/italic> in the North Central United States","volume":"8","author":"M Guerra","year":"2002","journal-title":"Emerg Infect Dis"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"Ruid D, Paul W, Roell B, Wydeven A, Willging R, Jurewicz R, et al. Wolf\u2013human conflicts and management in Minnesota, Wisconsin, and Michigan. In: Wydeven A, Van Deelen T, Heske E, editors. Recovery of gray wolves in the Great Lakes region of the United States. 2009. pp. 279\u2013295.","DOI":"10.1007\/978-0-387-85952-1_18"},{"key":"ref37","unstructured":"Jara RF. Spatial and temporal patterns of Gray Wolf exposure to vector-borne diseases in Wisconsin. M.Sc. Thesis, University of Wisconsin-Madison. 2013."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1080\/03610929708831995","article-title":"A spatial scan statistic","volume":"26","author":"M Kulldorff","year":"1997","journal-title":"Commun Stat methods"},{"key":"ref39","unstructured":"American Veterinary Medical Association. U.S. pet ownership statistics. 2013. Available: https:\/\/www.avma.org\/KB\/Resources\/Statistics\/Pages\/Market-research-statistics-US-pet-ownership.aspx<\/ext-link>. Accessed June 2013."},{"key":"ref40","unstructured":"IDEXX L. Diseases in your area. 2013. Available: http:\/\/www.dogsandticks.com\/diseases_in_your_area.php<\/ext-link>. Accessed June 2013."},{"key":"ref41","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1128\/IAI.69.5.3359-3371.2001","article-title":"Coinfection with Borrelia burgdorferi<\/italic> and the agent of human granulocytic ehrlichiosis alters murine immune responses, pathogen burden, and severity of Lyme arthritis","volume":"69","author":"V Thomas","year":"2001","journal-title":"Infect Immun"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.3201\/eid1112.050898","article-title":"Human granulocytic anaplasmosis and Anaplasma phagocytophilum<\/italic>","volume":"11","author":"J Dumler","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1089\/vbz.2008.0072","article-title":"Meta-analysis of coinfection and coexposure with Borrelia burgdorferi<\/italic> and Anaplasma phagocytophilum<\/italic> in humans, domestic animals, wildlife, and Ixodes ricinus<\/italic>-complex ticks","volume":"9","author":"NC Nieto","year":"2009","journal-title":"Vector-Borne Zoonotic Dis"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/jmedent\/43.2.166","article-title":"Spatiotemporal patterns of host-seeking Ixodes scapularis<\/italic> nymphs (Acari: Ixodidae) in the United States","volume":"43","author":"M Diuk-Wasser","year":"2006","journal-title":"J Med Entomol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1603\/ME12234","article-title":"Hunter-killed deer surveillance to assess changes in the prevalence and distribution of Ixodes scapularis<\/italic> (Acari: Ixodidae) in Wisconsin","volume":"50","author":"X Lee","year":"2013","journal-title":"J Med Entomol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1093\/jmedent\/7.1.124","article-title":"Ixodes scapularis<\/italic> Say in northern Wisconsin","volume":"7","author":"J Jackson","year":"1970","journal-title":"J Med Entomol"},{"key":"ref47","unstructured":"Wisconsin Department of Natural Resources. Fall deer population density 2012, per square mile deer range. 2012. Available: http:\/\/dnr.wi.gov\/topic\/hunt\/documents\/falldeerperdr.pdf<\/ext-link>. Accessed October 2013."},{"key":"ref48","doi-asserted-by":"crossref","first-page":"180","DOI":"10.4269\/ajtmh.1987.37.180","article-title":"Lyme disease ecology in Wisconsin: distribution and host preferences of Ixodes dammini<\/italic>, and prevalence of antibody to Borrelia burgdorferi<\/italic> in small mammals","volume":"37","author":"JM Godsey","year":"1987","journal-title":"Am J Trop Med Hyg"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1056\/NEJMoa1010493","article-title":"Emergence of a new pathogenic Ehrlichia species, Wisconsin and Minnesota, 2009","volume":"365","author":"B Pritt","year":"2011","journal-title":"N Engl J Med"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.3201\/eid2110.150143","article-title":"Human infection with Ehrlichia muris<\/italic>-like pathogen, United States, 2007\u20132013","volume":"21","author":"D Johnson","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"18","DOI":"10.2174\/1874285801105010018","article-title":"Prevalence of Ehrlichia muris<\/italic> in Wisconsin deer ticks collected during the mid 1990s","volume":"5","author":"S Telford III","year":"2011","journal-title":"Open Microbiol J"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1111\/j.1939-1676.2012.00968.x","article-title":"Erlichia muris<\/italic> infection in a dog from Minnesota","volume":"26","author":"B Hegarty","year":"2012","journal-title":"J Vet Intern Med"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"308","DOI":"10.7589\/2012-06-150","article-title":"Blood collected on filter paper for wildlife serology: Evaluating storage and temperature challenges of field collections","volume":"40","author":"P Curry","year":"2014","journal-title":"J Wildl Dis"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1128\/IAI.73.6.3440-3444.2005","article-title":"Coinfection with Anaplasma phagocytophilum<\/italic> alters Borrelia burgdorferi<\/italic> population distribution in C3H\/HeN mice","volume":"73","author":"K Holden","year":"2005","journal-title":"Infect Immun"},{"key":"ref55","unstructured":"IDEXX L. SNAP 4Dx Test Clinical Reference Guide. 2013. Available: http:\/\/www.idexx.com\/pubwebresources\/pdf\/en_us\/smallanimal\/snap\/4dx\/snap-4dx-clinical-reference-guide.pdf<\/ext-link>. Accessed November 2013."},{"key":"ref56","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.2460\/ajvr.1991.52.09.1537","article-title":"Evaluation of Rhipicephalus sanguineus<\/italic> as a potential biologic vector of Ehrlichia platys<\/italic>","volume":"52","author":"R Simpson","year":"1991","journal-title":"Am J Vet Res"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1756-3305-3-33","article-title":"Experimental infection and co-infection of dogs with Anaplasma platys<\/italic> and Ehrlichia canis<\/italic>: hematologic, serologic and molecular findings","volume":"3","author":"S Gaunt","year":"2010","journal-title":"Parasit Vectors"},{"key":"ref58","first-page":"119","article-title":"Recovery of gray wolves in the Great Lakes Region of the United States","author":"D Mladenoff","year":"2009"},{"key":"ref59","unstructured":"Centers for Disease Control and Prevention. Anaplasmosis, statistics and epidemiology. 2013. Available: http:\/\/www.cdc.gov\/anaplasmosis\/stats\/<\/ext-link>. Accessed October 2013."},{"key":"ref60","unstructured":"Wisconsin Department of Health Services. Lyme disease (Borrelia burgdorferi infection). 2013. Available: http:\/\/www.dhs.wisconsin.gov\/communicable\/Tickborne\/Lyme\/Index.htm<\/ext-link>. Accessed October 2013."},{"key":"ref61","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1756-3305-3-26","article-title":"Biology and ecology of the brown dog tick, Rhipicephalus sanguineus<\/italic>","volume":"3","author":"F Dantas-Torres","year":"2010","journal-title":"Parasit Vectors"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"206","DOI":"10.2460\/ajvr.67.2.206","article-title":"Comparison of an indirect immunofluorescence assay, western blot analysis, and a commercially available ELISA for detection of Ehrlichia canis<\/italic> antibodies in canine sera","volume":"67","author":"T O\u2019Connor","year":"2006","journal-title":"Am J Vet Res"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"929","DOI":"10.2460\/ajvr.1990.51.06.929","article-title":"Experimental transmission of a granulocytic form of the tribe Ehrlichieae by Dermacentor variabilis<\/italic> and Amblyomma americanum<\/italic> to dogs","volume":"51","author":"O Anziani","year":"1990","journal-title":"Am J Vet Res"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"239","DOI":"10.4269\/ajtmh.1993.49.239","article-title":"Amblyomma americanum<\/italic>: a potential vector of human ehrlichiosis","volume":"49","author":"B Anderson","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"ref65","unstructured":"Centers for Disease Control and Prevention. Approximate Distribution of the Lone Star Tick. 2011. Available: http:\/\/www.cdc.gov\/ticks\/maps\/lone_star_tick.html<\/ext-link>. Accessed October 2013."},{"key":"ref66","unstructured":"Wisconsin Department of Health Services. Ehrlichia chaffeensis infection. 2012. Available: http:\/\/www.dhs.wisconsin.gov\/communicable\/tickborne\/AandE\/Echaffdatamaps.htm<\/ext-link>. Accessed October 2013."},{"key":"ref67","first-page":"192","article-title":"Wolves:Behavior, Ecology and Conservation","author":"T Kreeger","year":"2003"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"177","DOI":"10.7589\/0090-3558-28.2.177","article-title":"Seroprevalence of Lyme disease in gray wolves from Minnesota and Wisconsin","volume":"28","author":"A Thieking","year":"1992","journal-title":"J Wildl Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0165836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T22:45:10Z","timestamp":1749768310000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0165836"}},"subtitle":[],"editor":[{"given":"Brian","family":"Stevenson","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,11,29]]},"references-count":68,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2016,11,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0165836","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2016,11,29]]}}}