{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:07:38Z","timestamp":1768468058878,"version":"3.49.0"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81641079"],"award-info":[{"award-number":["81641079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81171693"],"award-info":[{"award-number":["81171693"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672133"],"award-info":[{"award-number":["81672133"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National High Technology Research and Development Program of China","award":["SS2015AA020304"],"award-info":[{"award-number":["SS2015AA020304"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0167222","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T20:17:39Z","timestamp":1480364259000},"page":"e0167222","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Convenient In Vivo Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function"],"prefix":"10.1371","volume":"11","author":[{"given":"Wen","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Can","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao","family":"Hai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohong","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijie","family":"Leng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingsheng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunli","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,11,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1007\/s11914-011-0078-8","article-title":"Bone development: overview of bone cells and signaling","volume":"9","author":"A Teti","year":"2011","journal-title":"Curr Osteoporos Rep"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/nri2062","article-title":"Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems","volume":"7","author":"H Takayanagi","year":"2007","journal-title":"Nat Rev Immunol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.bone.2011.10.006","article-title":"Foreword: interactions between bone and adipose tissue and metabolism","volume":"50","author":"C Rosen","year":"2012","journal-title":"Bone"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/nature10763","article-title":"The contribution of bone to whole-organism physiology","volume":"481","author":"G Karsenty","year":"2012","journal-title":"Nature"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.cell.2010.06.003","article-title":"Insulin signaling in osteoblasts integrates bone remodeling and energy metabolism","volume":"142","author":"M Ferron","year":"2010","journal-title":"Cell"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.bone.2009.11.006","article-title":"Osteocyte apoptosis and control of bone resorption following ovariectomy in mice","volume":"46","author":"KB Emerton","year":"2010","journal-title":"Bone"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s11914-010-0028-x","article-title":"The role of FSH and TSH in bone loss and its clinical relevance","volume":"8","author":"M Agrawal","year":"2010","journal-title":"Curr Osteoporos Rep"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.tem.2009.02.001","article-title":"Bone as an endocrine organ","volume":"20","author":"S Fukumoto","year":"2009","journal-title":"Trends Endocrinol Metab"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/mabi.200900107","article-title":"Vascularization in bone tissue engineering: physiology, current strategies, major hurdles and future challenges","volume":"10","author":"MI Santos","year":"2010","journal-title":"Macromol Biosci"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.tibtech.2009.07.001","article-title":"Cell-to-cell communication between osteogenic and endothelial lineages: implications for tissue engineering","volume":"27","author":"M Grellier","year":"2009","journal-title":"Trends Biotechnol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"E38","DOI":"10.1152\/ajpendo.00008.2006","article-title":"Pathogenic role of Fgf23 in Hyp mice","volume":"291","author":"S Liu","year":"2006","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/j.imbio.2016.06.007","article-title":"Modeling complement-driven diseases in transgenic mice: values and limitations","volume":"221","author":"Y Ueda","year":"2016","journal-title":"Immunobiology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/ng944","article-title":"Efficient delivery of siRNA for inhibition of gene expression in postnatal mice","volume":"32","author":"DL Lewis","year":"2002","journal-title":"Nat Genet"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1126\/science.1064023","article-title":"Argonaute2, a link between genetic and biochemical analyses of RNAi","volume":"293","author":"SM Hammond","year":"2001","journal-title":"Science"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1038\/gt.2011.56","article-title":"Special delivery: targeted therapy with small RNAs","volume":"18","author":"D Peer","year":"2011","journal-title":"Gene Ther"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.semcdb.2015.12.004","article-title":"Interplay between CaSR and PTH1R signaling in skeletal development and osteoanabolism","volume":"49","author":"C Santa Maria","year":"2016","journal-title":"Semin Cell Dev Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fendo.2013.00006","article-title":"Anabolic effects of IGF-1 signaling on the skeleton","volume":"4","author":"CG Tahimic","year":"2013","journal-title":"Front Endocrinol (Lausanne)"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s00198-015-3230-y","article-title":"A single CT-guided percutaneous intraosseous injection of thermosensitive simvastatin\/poloxamer 407 hydrogel enhances vertebral bone formation in ovariectomized minipigs","volume":"27","author":"J Tan","year":"2016","journal-title":"Osteoporos Int"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3177\/jnsv.60.152","article-title":"The relationship between salivary calcium concentration and differences in bone mineral density level in female rats","volume":"60","author":"S Hattori","year":"2014","journal-title":"J Nutr Sci Vitaminol (Tokyo)"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1002\/jbmr.141","article-title":"Guidelines for assessment of bone microstructure in rodents using micro-computed tomography","volume":"25","author":"ML Bouxsein","year":"2010","journal-title":"J Bone Miner Res"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1002\/jor.23128","article-title":"Administration duration influences the effects of low-magnitude, high-frequency vibration on ovariectomized rat bone","volume":"34","author":"F Qing","year":"2016","journal-title":"J Orthop Res"},{"key":"ref22","article-title":"Statin-induced calcific Achilles tendinopathy in rats: comparison of biomechanical and histopathological effects of simvastatin, atorvastatin and rosuvastatin","author":"F Kaleagasioglu","year":"2015","journal-title":"Knee Surg Sports Traumatol Arthrosc"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1563\/AAID-JOI-D-09-00090","article-title":"Effect of dehiscences to the bone response of implants with an acid-etched surface: an experimental study in miniature pigs","volume":"37","author":"B Ruehe","year":"2011","journal-title":"J Oral Implantol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"6400","DOI":"10.1016\/j.biomaterials.2010.04.037","article-title":"Synergistic induction of bone formation by hOP-1, hTGF-beta3 and inhibition by zoledronate in macroporous coral-derived hydroxyapatites","volume":"31","author":"U Ripamonti","year":"2010","journal-title":"Biomaterials"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1210\/endo-125-3-1142","article-title":"Effects of interleukin-1 on bone turnover in normal mice","volume":"125","author":"BF Boyce","year":"1989","journal-title":"Endocrinology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"7993","DOI":"10.1073\/pnas.130511497","article-title":"Requirement of the inducible nitric oxide synthase pathway for IL-1-induced osteoclastic bone resorption","volume":"97","author":"RJ van't Hof","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1126\/science.286.5446.1946","article-title":"Stimulation of bone formation in vitro and in rodents by statins","volume":"286","author":"G Mundy","year":"1999","journal-title":"Science"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"243S","DOI":"10.1016\/8756-3282(95)00298-R","article-title":"An <italic>in-vivo<\/italic> model for the rapid assessment of skeletal effects of anabolic agents","volume":"17","author":"XJ Li","year":"1995","journal-title":"Bone"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.12659\/MSM.892247","article-title":"Single-dose local simvastatin injection improves implant fixation via increased angiogenesis and bone formation in an ovariectomized rat model","volume":"21","author":"J Tan","year":"2015","journal-title":"Med Sci Monit"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/s00774-013-0496-z","article-title":"Local injection of a single dose of simvastatin augments osteoporotic bone mass in ovariectomized rats","volume":"32","author":"N Yang","year":"2014","journal-title":"J Bone Miner Metab"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1208\/s12248-009-9140-1","article-title":"Lipidic systems for <italic>in vivo<\/italic> siRNA delivery","volume":"11","author":"SY Wu","year":"2009","journal-title":"AAPS J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1016\/j.addr.2012.03.013","article-title":"Gene delivery to bone","volume":"64","author":"CH Evans","year":"2012","journal-title":"Adv Drug Deliv Rev"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"147","DOI":"10.5483\/BMBRep.2015.48.3.089","article-title":"New paradigms on siRNA local application","volume":"48","author":"M Pan","year":"2015","journal-title":"BMB Rep"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"3805","DOI":"10.2147\/IJN.S48950","article-title":"Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-alpha and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line","volume":"8","author":"Y Wang","year":"2013","journal-title":"Int J Nanomedicine"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.ajem.2013.05.020","article-title":"The efficiency of intraosseous human growth hormone administration: a feasibility pilot study in a rabbit model","volume":"31","author":"M Massarwi","year":"2013","journal-title":"Am J Emerg Med"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.annemergmed.2012.05.013","article-title":"Rapid and complete bioavailability of antidotes for organophosphorus nerve agent and cyanide poisoning in minipigs after intraosseous administration","volume":"60","author":"DB Murray","year":"2012","journal-title":"Ann Emerg Med"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.resuscitation.2011.07.041","article-title":"Pharmacokinetics of intraosseous and central venous drug delivery during cardiopulmonary resuscitation","volume":"83","author":"SL Hoskins","year":"2012","journal-title":"Resuscitation"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0168-3659(03)00094-4","article-title":"Transdermal delivery of insulin from poloxamer gel: ex vivo and <italic>in vivo<\/italic> skin permeation studies in rat using iontophoresis and chemical enhancers","volume":"89","author":"O Pillai","year":"2003","journal-title":"J Control Release"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0167222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T07:45:45Z","timestamp":1589010345000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0167222"}},"subtitle":[],"editor":[{"given":"Dominique","family":"Heymann","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,11,28]]},"references-count":38,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2016,11,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0167222","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,28]]}}}