{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:00:00Z","timestamp":1793253600950,"version":"4.5.0"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T00:00:00Z","timestamp":1481500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kennel Club Charitable Trust"},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M009513\/1"],"award-info":[{"award-number":["BB\/M009513\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0168296","type":"journal-article","created":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T18:57:05Z","timestamp":1481569025000},"page":"e0168296","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Characterisation of the Immunophenotype of Dogs with Primary Immune-Mediated Haemolytic Anaemia"],"prefix":"10.1371","volume":"11","author":[{"given":"James W.","family":"Swann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Woods","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Glanemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver A.","family":"Garden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,12,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1016\/j.cvsm.2003.08.003","article-title":"Immune-mediated hemolytic anemia: understanding the nemesis","volume":"33","author":"S McCullough","year":"2003","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"363278","DOI":"10.1155\/2015\/363278","article-title":"Red blood cell destruction in autoimmune hemolytic anemia: role of complement and potential new targets for therapy","volume":"2015","author":"S Berentsen","year":"2015","journal-title":"Biomed Res Int"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1365-2249.1991.tb05678.x","article-title":"Identification of autoantigens in canine autoimmune haemolytic anaemia","volume":"85","author":"RN Barker","year":"1991","journal-title":"Clin Exp Immunol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0165-2427(97)00032-9","article-title":"Proliferative responses of peripheral blood mononuclear cells from normal dogs and dogs with autoimmune haemolytic anaemia to red blood cell antigens","volume":"59","author":"A Corato","year":"1997","journal-title":"Vet Immunol Immunopathol"},{"issue":"Suppl 4","key":"ref5","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1186\/ar3918","article-title":"B-cell selection and the development of autoantibodies","volume":"14","author":"NV Giltiay","year":"2012","journal-title":"Arthritis Res Ther"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1093\/intimm\/dxp095","article-title":"Regulatory T cells: how do they suppress immune responses?","volume":"21","author":"S Sakaguchi","year":"2009","journal-title":"Int Immunol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.intimp.2010.11.003","article-title":"All creatures great and small: regulatory T cells in mice, humans, dogs and other domestic animal species","volume":"11","author":"OA Garden","year":"2011","journal-title":"Int Immunopharmacol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1365-2567.2010.03346.x","article-title":"Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog","volume":"132","author":"D Pinheiro","year":"2011","journal-title":"Immunology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1126\/science.1160062","article-title":"CTLA-4 control over Foxp3+ regulatory T cell function","volume":"322","author":"K Wing","year":"2008","journal-title":"Science"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1172\/JCI200522433","article-title":"The IL-6R alpha chain controls lung CD4+CD25+ Treg development and function during allergic airway inflammation in vivo","volume":"115","author":"A Doganci","year":"2005","journal-title":"J Clin Invest"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1182\/blood-2005-11-4567","article-title":"TNF downmodulates the function of human CD4+CD25hi T-regulatory cells","volume":"108","author":"X Valencia","year":"2006","journal-title":"Blood"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/1074-7613(95)90180-9","article-title":"Interleukin-2 receptor alpha chain regulates the size and content of the peripheral lymphoid compartment","volume":"3","author":"DM Willerford","year":"1995","journal-title":"Immunity"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1038\/ni.1791","article-title":"Interleukin 10 acts on regulatory T cells to maintain expression of the transcription factor Foxp3 and suppressive function in mice with colitis","volume":"10","author":"M Murai","year":"2009","journal-title":"Nat Immunol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0092-8674(93)80067-O","article-title":"Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene","volume":"75","author":"B Sadlack","year":"1993","journal-title":"Cell"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3109\/08830180009048392","article-title":"Chronic colitis in IL-10-\/- mice: insufficient counter regulation of a Th1 response","volume":"19","author":"NJ Davidson","year":"2000","journal-title":"Int Rev Immunol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.tvjl.2015.10.022","article-title":"Novel immunotherapies for immune-mediated haemolytic anaemia in dogs and people","volume":"207","author":"JW Swann","year":"2016","journal-title":"Vet J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.3324\/haematol.2014.114561","article-title":"Treatment of autoimmune hemolytic anemias","volume":"99","author":"A Zanella","year":"2014","journal-title":"Haematologica"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"SA Bustin","year":"2009","journal-title":"Clin Chem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e0137377","DOI":"10.1371\/journal.pone.0137377","article-title":"Low Vitamin D Status Is Associated with Systemic and Gastrointestinal Inflammation in Dogs with a Chronic Enteropathy","volume":"10","author":"HF Titmarsh","year":"2015","journal-title":"PloS one"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.jclinepi.2007.11.008","article-title":"The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement: guidelines for reporting observational studies","volume":"61","author":"E von Elm","year":"2008","journal-title":"J Clin Epidemiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/nri2889","article-title":"Mechanisms of impaired regulation by CD4(+)CD25(+)FOXP3(+) regulatory T cells in human autoimmune diseases","volume":"10","author":"JH Buckner","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF00344296","article-title":"Genetic susceptibility to post-thymectomy autoimmune diseases in mice","volume":"14","author":"A Kojima","year":"1981","journal-title":"Immunogenetics"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/83784","article-title":"Disruption of a new forkhead\/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse","volume":"27","author":"ME Brunkow","year":"2001","journal-title":"Nat Genet"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/ni1428","article-title":"Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice","volume":"8","author":"JM Kim","year":"2007","journal-title":"Nat Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/imm.12065","article-title":"Transient depletion of CD4+ CD25+ regulatory T cells results in multiple autoimmune diseases in wild-type and B-cell-deficient NOD mice","volume":"139","author":"JS Ellis","year":"2013","journal-title":"Immunology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/83713","article-title":"The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3","volume":"27","author":"CL Bennett","year":"2001","journal-title":"Nat Genet"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/83707","article-title":"X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome is the human equivalent of mouse scurfy","volume":"27","author":"RS Wildin","year":"2001","journal-title":"Nat Genet"},{"key":"ref28","first-page":"297","article-title":"Naturally occurring regulatory T cells and interleukins 10 and 12 in the pathogenesis of idiopathic warm autoimmune hemolytic anemia","volume":"21","author":"E Ahmad","year":"2011","journal-title":"J Investig Allergol Clin Immunol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"3746","DOI":"10.1182\/blood-2004-12-4692","article-title":"CD4+CD25+ regulatory T cells control induction of autoimmune hemolytic anemia","volume":"105","author":"A Mqadmi","year":"2005","journal-title":"Blood"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"R335","DOI":"10.1186\/ar1192","article-title":"CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease","volume":"6","author":"D Cao","year":"2004","journal-title":"Arthritis Res Ther"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1093\/rheumatology\/kel089","article-title":"Early rheumatoid arthritis is associated with a deficit in the CD4+CD25high regulatory T cell population in peripheral blood","volume":"45","author":"CA Lawson","year":"2006","journal-title":"Rheumatology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1080\/03009740701482800","article-title":"Accumulation of FoxP3-expressing CD4+CD25+ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis","volume":"36","author":"Z Jiao","year":"2007","journal-title":"Scand J Rheumatol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.autrev.2011.05.004","article-title":"Human FoxP3+ regulatory T cells in systemic autoimmune diseases","volume":"10","author":"M Miyara","year":"2011","journal-title":"Autoimmun Rev"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1084\/jem.20031579","article-title":"Loss of functional suppression by CD4+CD25+ regulatory T cells in patients with multiple sclerosis","volume":"199","author":"V Viglietta","year":"2004","journal-title":"J Exp Med"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"92","DOI":"10.2337\/diabetes.54.1.92","article-title":"Defective suppressor function in CD4(+)CD25(+) T-cells from patients with type 1 diabetes","volume":"54","author":"S Lindley","year":"2005","journal-title":"Diabetes"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1538\/expanim.60.471","article-title":"Phenotypic and functional characterization of freshly isolated and expanded canine regulatory T cells","volume":"60","author":"A Knueppel","year":"2011","journal-title":"Exp Anim"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"4529","DOI":"10.1182\/blood-2002-05-1383","article-title":"Interleukin-10-mediated regulatory T-cell responses to epitopes on a human red blood cell autoantigen","volume":"100","author":"AM Hall","year":"2002","journal-title":"Blood"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1182\/blood-2007-07-101345","article-title":"Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia","volume":"111","author":"FJ Ward","year":"2008","journal-title":"Blood"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1939-1676.2010.0642.x","article-title":"Use of serum concentrations of interleukin-18 and monocyte chemoattractant protein-1 as prognostic indicators in primary immune-mediated hemolytic anemia in dogs","volume":"25","author":"M Kjelgaard-Hansen","year":"2011","journal-title":"J Vet Intern Med"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"449","DOI":"10.3109\/08820139409066839","article-title":"High cytokine serum levels in patients with autoimmune hemolytic anemia (AIHA)","volume":"23","author":"E Fagiolo","year":"1994","journal-title":"Immunol Invest"},{"issue":"3","key":"ref41","doi-asserted-by":"crossref","first-page":"147","DOI":"10.3109\/08916939608995360","article-title":"Lymphocyte activation and cytokine production in autoimmune hemolytic anaemia (AIHA)","volume":"24","author":"E Fagiolo","year":"1996","journal-title":"Autoimmunity"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/S0952-7915(00)00160-6","article-title":"Helper T cells in antibody-mediated, organ-specific autoimmunity","volume":"12","author":"CJ Elson","year":"2000","journal-title":"Curr Opin Immunol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s11684-015-0388-9","article-title":"Th17 cells in autoimmune diseases","volume":"9","author":"L Han","year":"2015","journal-title":"Front Med"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.3324\/haematol.2011.060822","article-title":"Production of the effector cytokine interleukin-17, rather than interferon-gamma, is more strongly associated with autoimmune hemolytic anemia","volume":"97","author":"AM Hall","year":"2012","journal-title":"Haematologica"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1016\/j.exphem.2012.08.008","article-title":"Critical role of Th17 cells in development of autoimmune hemolytic anemia","volume":"40","author":"L Xu","year":"2012","journal-title":"Exp Hematol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1189\/jlb.71.1.1","article-title":"IL-17: prototype member of an emerging cytokine family","volume":"71","author":"S Aggarwal","year":"2002","journal-title":"J Leukoc Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"347","DOI":"10.3109\/08820139909062268","article-title":"Enhanced IL-10 production in vitro by monocytes in autoimmune haemolytic anaemia","volume":"28","author":"E Fagiolo","year":"1999","journal-title":"Immunol Invest"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1080\/08916930290005891","article-title":"Th1 and Th2 cytokine modulation by IL-10\/IL-12 imbalance in autoimmune haemolytic anaemia (AIHA)","volume":"35","author":"E Fagiolo","year":"2002","journal-title":"Autoimmunity"},{"key":"ref49","first-page":"421","article-title":"Spontaneous production of interleukin-10 by B lymphocytes and monocytes in systemic lupus erythematosus","volume":"4","author":"L Llorente","year":"1993","journal-title":"Eur Cytokine Netw"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1084\/jem.181.3.839","article-title":"Role of interleukin 10 in the B lymphocyte hyperactivity and autoantibody production of human systemic lupus erythematosus","volume":"181","author":"L Llorente","year":"1995","journal-title":"J Exp Med"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/S0531-5565(03)00189-X","article-title":"The effect of age and gender on cytokine production by human peripheral blood mononuclear cells and markers of bone metabolism","volume":"38","author":"P Pietschmann","year":"2003","journal-title":"Exp Gerontol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.2174\/092986707780831131","article-title":"Ethnicity and inflammatory pathways\u2014implications for vascular disease, vascular risk and therapeutic intervention","volume":"14","author":"MA Miller","year":"2007","journal-title":"Curr Med Chem"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"434010","DOI":"10.1155\/2013\/434010","article-title":"Cytokine levels in the serum of healthy subjects","volume":"2013","author":"G Kleiner","year":"2013","journal-title":"Mediators Inflamm"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/0090-1229(92)90057-U","article-title":"A canine febrile disorder associated with elevated interleukin-6","volume":"64","author":"AL Rivas","year":"1992","journal-title":"Clin Immunol Immunopathol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.cyto.2015.01.009","article-title":"Seeking balance: Potentiation and inhibition of multiple sclerosis autoimmune responses by IL-6 and IL-10","volume":"73","author":"SJ Ireland","year":"2015","journal-title":"Cytokine"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.cyto.2014.10.031","article-title":"Interleukin-10 paradox: A potent immunoregulatory cytokine that has been difficult to harness for immunotherapy","volume":"74","author":"A Saxena","year":"2015","journal-title":"Cytokine"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.2460\/javma.2001.218.1308","article-title":"Correlation between leukocytosis and necropsy findings in dogs with immune-mediated hemolytic anemia: 34 cases (1994\u20131999)","volume":"218","author":"PM McManus","year":"2001","journal-title":"J Am Vet Med Assoc"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/j.1939-1676.2009.0282.x","article-title":"Serum acute phase protein concentrations in dogs with autoimmune hemolytic anemia","volume":"23","author":"KD Mitchell","year":"2009","journal-title":"J Vet Intern Med"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.vetimm.2011.08.010","article-title":"High intravascular tissue factor expression in dogs with idiopathic immune-mediated haemolytic anaemia","volume":"144","author":"CJ Piek","year":"2011","journal-title":"Vet Immunol Immunopathol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/nri3062","article-title":"Inflammatory modulation of HSCs: viewing the HSC as a foundation for the immune response","volume":"11","author":"KY King","year":"2011","journal-title":"Nat Rev Immunol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0168296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T03:48:25Z","timestamp":1692589705000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0168296"}},"subtitle":[],"editor":[{"given":"Douglas","family":"Thamm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,12,12]]},"references-count":60,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2016,12,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0168296","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,12]]}}}