{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T03:06:47Z","timestamp":1771556807548,"version":"3.50.1"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2017,6,7]],"date-time":"2017-06-07T00:00:00Z","timestamp":1496793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0168702","type":"journal-article","created":{"date-parts":[[2017,6,7]],"date-time":"2017-06-07T13:25:19Z","timestamp":1496841919000},"page":"e0168702","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Single dose primaquine to reduce gametocyte carriage and Plasmodium falciparum transmission in Cambodia: An open-label randomized trial"],"prefix":"10.1371","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4516-723X","authenticated-orcid":true,"given":"Jessica T.","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanthap","family":"Lon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele D.","family":"Spring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somethy","family":"Sok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soklyda","family":"Chann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mali","family":"Ittiverakul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Worachet","family":"Kuntawunginn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mok","family":"My","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kheangheng","family":"Thay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rifat","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujata","family":"Balasubramanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengchuor","family":"Char","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte A.","family":"Lanteri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panita","family":"Gosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ratawan","family":"Ubalee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven R.","family":"Meshnick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Saunders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,6,7]]},"reference":[{"key":"ref1","unstructured":"World Health Organization. Global malaria control and elimination. Report of a meeting on containment of artemisinin tolerance, April 2008. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/malaria\/publications\/atoz\/9789241596817\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/malaria\/publications\/atoz\/9789241596817\/en\/<\/ext-link>."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1186\/1475-2875-13-385","article-title":"Spatial and temporal epidemiology of clinical malaria in Cambodia 2004\u20132013","volume":"13","author":"RJ Maude","year":"2014","journal-title":"Malar J"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1056\/NEJMoa1314981","article-title":"Spread of artemisinin resistance in Plasmodium falciparum malaria","volume":"371","author":"EA Ashley","year":"2014","journal-title":"N Engl J Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1056\/NEJMc1403007","article-title":"Dihydroartemisinin-piperaquine failure in Cambodia","volume":"371","author":"DL Saunders","year":"2014","journal-title":"N Engl J Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S1473-3099(15)70049-6","article-title":"Dihydroartemisinin-piperaquine failure associated with a triple mutant including kelch13 C580Y in Cambodia: an observational cohort study","volume":"15","author":"MD Spring","year":"2015","journal-title":"Lancet Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"4719","DOI":"10.1128\/AAC.00835-15","article-title":"Evidence of Plasmodium falciparum Malaria Multidrug Resistance to Artemisinin and Piperaquine in Western Cambodia: Dihydroartemisinin-Piperaquine Open-Label Multicenter Clinical Assessment","volume":"59","author":"R Leang","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"ref7","article-title":"Guidelines for the Treatment of Malaria, 2010","year":"2010"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S1473-3099(10)70187-0","article-title":"Effectiveness of five artemisinin combination regimens with or without primaquine in uncomplicated falciparum malaria: an open-label randomised trial","volume":"10","author":"F Smithuis","year":"2010","journal-title":"Lancet Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e1023","DOI":"10.1371\/journal.pone.0001023","article-title":"Primaquine clears submicroscopic Plasmodium falciparum gametocytes that persist after treatment with sulphadoxine-pyrimethamine and artesunate","volume":"2","author":"S Shekalaghe","year":"2007","journal-title":"PloS One"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1128\/AAC.48.4.1329-1334.2004","article-title":"Activities of artesunate and primaquine against asexual- and sexual-stage parasites in falciparum malaria","volume":"48","author":"S Pukrittayakamee","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1186\/1475-2875-9-136","article-title":"Revisiting the circulation time of Plasmodium falciparum gametocytes: molecular detection methods to estimate the duration of gametocyte carriage and the effect of gametocytocidal drugs","volume":"9","author":"T Bousema","year":"2010","journal-title":"Malar J"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1473-3099(12)70198-6","article-title":"Primaquine to prevent transmission of falciparum malaria","volume":"13","author":"NJ White","year":"2013","journal-title":"Lancet Infect Dis"},{"key":"ref13","first-page":"709","article-title":"The infectivity of gametocytes of Plasmodium falciparum from patients treated with artemisinin","volume":"107","author":"PQ Chen","year":"1994","journal-title":"Chin Med J (Engl)"},{"key":"ref14","article-title":"Primaquine to reduce transmission of Plasmodium falciparum malaria in Mali: a single-blind, dose-ranging, adaptive randomised phase 2 trial","author":"A Dicko","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e19283","DOI":"10.1371\/journal.pone.0019283","article-title":"Artesunate dose escalation for the treatment of uncomplicated malaria in a region of reported artemisinin resistance: a randomized clinical trial","volume":"6","author":"D Bethell","year":"2011","journal-title":"PloS One"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"114","DOI":"10.4269\/ajtmh.2008.78.114","article-title":"A filter paper method for the detection of Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction","volume":"78","author":"G Mlambo","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e18716","DOI":"10.1371\/journal.pone.0018716","article-title":"Plasmodium falciparum gametocyte carriage is associated with subsequent Plasmodium vivax relapse after treatment","volume":"6","author":"JT Lin","year":"2011","journal-title":"PloS One"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1093\/infdis\/jiv599","article-title":"Microscopic Plasmodium falciparum Gametocytemia and Infectivity to Mosquitoes in Cambodia","volume":"213","author":"JT Lin","year":"2016","journal-title":"J Infect Dis"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s12916-016-0581-y","article-title":"Single low dose primaquine to reduce gametocyte carriage and Plasmodium falciparum transmission after artemether-lumefantrine in children with asymptomatic infection: a randomised, double-blind, placebo-controlled trial","volume":"14","author":"BP Gon\u00e7alves","year":"2016","journal-title":"BMC Med"},{"issue":"Suppl 1","key":"ref20","first-page":"73","article-title":"Malaria vectors in the Greater Mekong Subregion: overview of malaria vectors and remaining challenges","volume":"44","author":"J Hii","year":"2013","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"ref21","unstructured":"World Health Organization. Updated WHO Policy REcommendation (October 2012): Single dose Primaquine as a gametocytocide in Plasmodium falciparum malaria. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/malaria\/pq_updated_policy_recommendation_en_102012.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/malaria\/pq_updated_policy_recommendation_en_102012.pdf<\/ext-link>. Accessed 20 May 2016."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1186\/1475-2875-11-418","article-title":"Rationale for recommending a lower dose of primaquine as a Plasmodium falciparum gametocytocide in populations where G6PD deficiency is common","volume":"11","author":"NJ White","year":"2012","journal-title":"Malar J"},{"key":"ref23","first-page":"349","article-title":"Acute intravascular haemolysis in Vanuatu following a single dose of primaquine in individuals with glucose-6-phosphate dehydrogenase deficiency","volume":"95","author":"PA Reeve","year":"1992","journal-title":"J Trop Med Hyg"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1186\/1475-2875-13-418","article-title":"Primaquine: the risks and the benefits","volume":"13","author":"EA Ashley","year":"2014","journal-title":"Malar J"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1128\/AAC.01135-09","article-title":"In Tanzania, hemolysis after a single dose of primaquine coadministered with an artemisinin is not restricted to glucose-6-phosphate dehydrogenase-deficient (G6PD A-) individuals","volume":"54","author":"SA Shekalaghe","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e0151898","DOI":"10.1371\/journal.pone.0151898","article-title":"Single Low Dose Primaquine (0.25mg\/kg) Does Not Cause Clinically Significant Haemolysis in G6PD Deficient Subjects","volume":"11","author":"G Bancone","year":"2016","journal-title":"PloS One"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S1473-3099(13)70268-8","article-title":"Single dose primaquine for clearance of Plasmodium falciparum gametocytes in children with uncomplicated malaria in Uganda: a randomised, controlled, double-blind, dose-ranging trial","volume":"14","author":"AC Eziefula","year":"2014","journal-title":"Lancet Infect Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3418","DOI":"10.1038\/srep03418","article-title":"The relevance and applicability of oocyst prevalence as a read-out for mosquito feeding assays","volume":"3","author":"WJR Stone","year":"2013","journal-title":"Sci Rep"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1186\/1475-2875-7-249","article-title":"Plasmodium falciparum gametocyte dynamics in areas of different malaria endemicity","volume":"7","author":"K Stepniewska","year":"2008","journal-title":"Malar J"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s12916-016-0621-7","article-title":"Gametocyte carriage in uncomplicated Plasmodium falciparum malaria following treatment with artemisinin combination therapy: a systematic review and meta-analysis of individual patient data","volume":"14","year":"2016","journal-title":"BMC Med"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e4551","DOI":"10.1371\/journal.pone.0004551","article-title":"Changes in the treatment responses to artesunate-mefloquine on the northwestern border of Thailand during 13 years of continuous deployment","volume":"4","author":"VI Carrara","year":"2009","journal-title":"PloS One"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1016\/S0140-6736(12)60484-X","article-title":"Emergence of artemisinin-resistant malaria on the western border of Thailand: a longitudinal study","volume":"379","author":"AP Phyo","year":"2012","journal-title":"Lancet Lond Engl"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1093\/cid\/cis959","article-title":"The effect of primaquine on gametocyte development and clearance in the treatment of uncomplicated falciparum malaria with dihydroartemisinin-piperaquine in South sumatra, Western indonesia: an open-label, randomized, controlled trial","volume":"56","author":"I Sutanto","year":"2013","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1093\/infdis\/jiw044","article-title":"Infectivity of Microscopic and Submicroscopic Malaria Parasite Infections in Areas of Low Malaria Endemicity","volume":"213","author":"BP Gon\u00e7alves","year":"2016","journal-title":"J Infect Dis"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1093\/infdis\/jiw045","article-title":"Reply to Goncalves et al","volume":"213","author":"JT Lin","year":"2016","journal-title":"J Infect Dis"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.pt.2014.02.004","article-title":"The role of submicroscopic parasitemia in malaria transmission: what is the evidence?","volume":"30","author":"JT Lin","year":"2014","journal-title":"Trends Parasitol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e6358","DOI":"10.1371\/journal.pone.0006358","article-title":"Safety and efficacy of dihydroartemisinin-piperaquine in falciparum malaria: a prospective multi-centre individual patient data analysis","volume":"4","author":"J Zwang","year":"2009","journal-title":"PloS One"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1093\/infdis\/jit077","article-title":"Malaria transmission after artemether-lumefantrine and dihydroartemisinin-piperaquine: a randomized trial","volume":"207","author":"P Sawa","year":"2013","journal-title":"J Infect Dis"},{"key":"ref39","unstructured":"Cambodia Ministry of Health. National Treatment Guidelines for Malaria in Cambodia, December 2014."},{"key":"ref40","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/S1473-3099(15)00525-3","article-title":"Combination therapies for malaria are failing-what next?","volume":"16","author":"D Saunders","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1186\/1475-2875-13-483","article-title":"Assessment of therapeutic responses to gametocytocidal drugs in Plasmodium falciparum malaria","volume":"13","author":"NJ White","year":"2014","journal-title":"Malar J"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.molbiopara.2015.03.006","article-title":"Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR","volume":"199","author":"P Schneider","year":"2015","journal-title":"Mol Biochem Parasitol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/0035-9203(49)90051-6","article-title":"Observations on the action of quinine, atebrin and plasmoquine on the gametocytes of Plasmodium falciparum","volume":"42","author":"MJ Mackerras","year":"1949","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1186\/s12936-015-0714-3","article-title":"An assessment of the supply, programmatic use, and regulatory issues of single low-dose primaquine as a Plasmodium falciparum gametocytocide for sub-Saharan Africa","volume":"14","author":"I Chen","year":"2015","journal-title":"Malar J"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0168702","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T14:39:37Z","timestamp":1569422377000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0168702"}},"subtitle":[],"editor":[{"given":"Aric","family":"Gregson","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,6,7]]},"references-count":44,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2017,6,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0168702","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,7]]}}}