{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T11:12:08Z","timestamp":1768821128651,"version":"3.49.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T00:00:00Z","timestamp":1482883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007405","name":"The Danish Heart Foundation","doi-asserted-by":"crossref","award":["14-R97-A5031-22857"],"award-info":[{"award-number":["14-R97-A5031-22857"]}],"id":[{"id":"10.13039\/100007405","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001734","name":"University of Copenhagen","doi-asserted-by":"crossref","award":["624-0013\/12-3000"],"award-info":[{"award-number":["624-0013\/12-3000"]}],"id":[{"id":"10.13039\/501100001734","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Unrestricted grant from LEO Foundation"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0168771","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T16:27:28Z","timestamp":1483979248000},"page":"e0168771","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Pseudomonas aeruginosa Microcolonies in Coronary Thrombi from Patients with ST-Segment Elevation Myocardial Infarction"],"prefix":"10.1371","volume":"11","author":[{"given":"Gorm M\u00f8rk","family":"Hansen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Belstr\u00f8m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Nilsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Helqvist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus Henrik","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Palle","family":"Holmstrup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Tolker-Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Givskov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter Riis","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,12,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1056\/NEJMra043430","article-title":"Inflammation, atherosclerosis, and coronary artery disease","volume":"352","author":"GK Hansson","year":"2005","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1016\/j.jacc.2009.09.009","article-title":"Inflammation in atherosclerosis: from pathophysiology to practice","volume":"54","author":"P Libby","year":"2009","journal-title":"J Am Coll Cardiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.arcmed.2015.05.006","article-title":"Infection and atherosclerosis development","volume":"46","author":"LA Campbell","year":"2015","journal-title":"Arch Med Res"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.amjcard.2016.05.036","article-title":"Relation of periodontitis to risk of cardiovascular and all-cause mortality (from a Danish nationwide cohort study)","volume":"118","author":"GM Hansen","year":"2016","journal-title":"Am J Cardiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"240","DOI":"10.12998\/wjcc.v2.i7.240","article-title":"Infectious burden and atherosclerosis: A clinical issue","volume":"2","author":"R Sessa","year":"2014","journal-title":"World J Clin Cases WJCC"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1160\/TH11-06-0392","article-title":"Pathogens and atherosclerosis: update on the potential contribution of multiple infectious organisms to the pathogenesis of atherosclerosis","volume":"106","author":"ME Rosenfeld","year":"2011","journal-title":"Thromb Haemost"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/nrrheum.2015.112","article-title":"Cardiovascular comorbidity in rheumatic diseases","volume":"11","author":"MT Nurmohamed","year":"2015","journal-title":"Nat Rev Rheumatol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.amjmed.2013.08.013","article-title":"Cardiovascular risk and psoriasis: beyond the traditional risk factors","volume":"127","author":"AG Coumbe","year":"2014","journal-title":"Am J Med"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.atherosclerosis.2015.04.819","article-title":"Interaction between periodontal disease and atherosclerotic vascular disease\u2013fact or fiction?","volume":"241","author":"G Aarabi","year":"2015","journal-title":"Atherosclerosis"},{"issue":"Suppl 4","key":"ref10","first-page":"S30","article-title":"Periodontal bacterial invasion and infection: contribution to atherosclerotic pathology","volume":"84","author":"L Reyes","year":"2013","journal-title":"J Periodontol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e97811","DOI":"10.1371\/journal.pone.0097811","article-title":"Active invasion of oral and aortic tissues by <italic>Porphyromonas gingivalis<\/italic> in mice causally links periodontitis and atherosclerosis","volume":"9","author":"IM Velsko","year":"2011","journal-title":"PLoS ONE"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e17","DOI":"10.1161\/01.ATV.0000155018.67835.1a","article-title":"Human atherosclerotic plaque contains viable invasive <italic>Actinobacillus actinomycetemcomitans<\/italic> and <italic>Porphyromonas gingivalis<\/italic>","volume":"25","author":"EV Kozarov","year":"2005","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e01206","DOI":"10.1128\/mBio.01206-14","article-title":"Bacteria present in carotid arterial plaques are found as biofilm deposits which may contribute to enhanced risk of plaque rupture","volume":"5","author":"BB Lanter","year":"2014","journal-title":"mBio"},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.1186\/s40168-015-0100-y","article-title":"In silico analyses of metagenomes from human atherosclerotic plaque samples","volume":"3","author":"S Mitra","year":"2015","journal-title":"Microbiome"},{"issue":"Suppl 2","key":"ref15","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.12968\/jowc.2016.25.Sup2.S16","article-title":"The presence of biofilm structures in atherosclerotic plaques of arteries from legs amputated as a complication of diabetic foot ulcers","volume":"25","author":"DE Snow","year":"2016","journal-title":"J Wound Care"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"3628","DOI":"10.1016\/j.jmb.2015.08.016","article-title":"<italic>Pseudomonas aeruginosa<\/italic> biofilm infections: community structure, antimicrobial tolerance and immune response","volume":"427","author":"M Rybtke","year":"2015","journal-title":"J Mol Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.tim.2013.06.002","article-title":"The <italic>in vivo<\/italic> biofilm","volume":"21","author":"T Bjarnsholt","year":"2013","journal-title":"Trends Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1161\/CIRCULATIONAHA.112.001254","article-title":"Bacterial signatures in thrombus aspirates of patients with myocardial infarction","volume":"127","author":"T Pessi","year":"2013","journal-title":"Circulation"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.ahj.2011.10.012","article-title":"Detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction","volume":"163","author":"T Ohki","year":"2012","journal-title":"Am Heart J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1093\/ehjci\/jeu180","article-title":"Coronary liposuction during percutaneous coronary intervention: evidence by near-infrared spectroscopy that aspiration reduces culprit lesion lipid content prior to stent placement","volume":"16","author":"D Erlinge","year":"2015","journal-title":"Eur Heart J Cardiovasc Imaging"},{"key":"ref21","first-page":"1","article-title":"Validity of self-reported periodontal disease: A systematic review and meta-analysis","volume":"13","author":"HM Abbood","year":"2016","journal-title":"J Periodontol"},{"key":"ref22","doi-asserted-by":"crossref","DOI":"10.1186\/2049-2618-2-6","article-title":"An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform","volume":"2","author":"DW Fadrosh","year":"2014","journal-title":"Microbiome"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/978-1-4939-1459-3_21","article-title":"PNA-based fluorescence <italic>in situ<\/italic> hybridization for identification of bacteria in clinical samples","volume":"1211","author":"M Fazli","year":"2014","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1093\/nar\/gkt1244","article-title":"Ribosomal Database Project: data and tools for high throughput rRNA analysis","volume":"42","author":"JR Cole","year":"2014","journal-title":"Nucl. Acids Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1128\/AEM.03006-05","article-title":"Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB","volume":"72","author":"TZ DeSantis","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The Human Oral Microbiome","volume":"192","author":"FE Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"ref27","first-page":"33","article-title":"Antibiotic trials for coronary heart disease","volume":"31","author":"JL Anderson","year":"2004","journal-title":"Tex Heart Inst J"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/jcpe.12190","article-title":"Differences in bacterial saliva profile between periodontitis patients and a control cohort","volume":"41","author":"D Belstr\u00f8m","year":"2014","journal-title":"J Clin Periodontol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1590\/S1517-83822014000200017","article-title":"Prevalence of <italic>Pseudomonas aeruginosa<\/italic> and <italic>Acinetobacter spp<\/italic>. in subgingival biofilm and saliva of subjects with chronic periodontal infection","volume":"45","author":"R Souto","year":"2014","journal-title":"Braz J Microbiol"},{"key":"ref30","article-title":"<italic>Pseudomonas aeruginosa<\/italic> renews its virulence factors","author":"P Huber","year":"2016","journal-title":"Environ Microbiol Rep"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1586\/erp.10.49","article-title":"Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes","volume":"10","author":"EB Hirsch","year":"2010","journal-title":"Expert Rev Pharmacoecon Outcomes Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1111\/jcmm.12778","article-title":"Phosphatidylserine externalization and procoagulant activation of erythrocytes induced by <italic>Pseudomonas aeruginosa<\/italic> virulence factor pyocyanin","volume":"20","author":"SM Qadri","year":"2016","journal-title":"J Cell Mol Med"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/S1286-4579(00)01259-4","article-title":"Establishment of <italic>Pseudomonas aeruginosa<\/italic> infection: lessons from a versatile opportunist","volume":"2","author":"JB Lyczak","year":"2000","journal-title":"Microbes Infect"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1111\/j.1469-0691.2004.00920.x","article-title":"Effect of chronic <italic>Pseudomonas aeruginosa<\/italic> infection on the development of atherosclerosis in a rat model","volume":"10","author":"C Turkay","year":"2004","journal-title":"Clin Microbiol Infect"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e109761","DOI":"10.1371\/journal.pone.0109761","article-title":"Microbial diversity similarities in periodontal pockets and atheromatous plaques of cardiovascular disease patients","volume":"9","author":"W Serra e Silva Filho","year":"2014","journal-title":"PLoS ONE"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e128","DOI":"10.1111\/odi.12205","article-title":"Microbial composition of atherosclerotic plaques","volume":"20","author":"C Calandrini","year":"2014","journal-title":"Oral Dis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1056\/NEJMra0904124","article-title":"Hospital-acquired infections due to Gram-negative bacteria","volume":"362","author":"AY Peleg","year":"2010","journal-title":"N Engl J Med"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"3500","DOI":"10.1099\/mic.0.031443-0","article-title":"Pseudomonas aeruginosa recognizes and responds aggressively to the presence of polymorphonuclear leukocytes","volume":"155","author":"M Alhede","year":"2009","journal-title":"Microbiol Read Engl"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1128\/IAI.06215-11","article-title":"Interactions between polymorphonuclear leukocytes and <italic>Pseudomonas aeruginosa<\/italic> biofilms on silicone implants <italic>in vivo<\/italic>","volume":"80","author":"M van Gennip","year":"2012","journal-title":"Infect Immun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1111\/j.1600-0463.2009.02466.x","article-title":"Inactivation of the rhlA gene in <italic>Pseudomonas aeruginosa<\/italic> prevents rhamnolipid production, disabling the protection against polymorphonuclear leukocytes","volume":"117","author":"M van Gennip","year":"2009","journal-title":"APMIS Acta Pathol Microbiol Immunol Scand"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1128\/AAC.00001-13","article-title":"Extracellular DNA shields against aminoglycosides in <italic>Pseudomonas aeruginosa<\/italic> biofilms","volume":"57","author":"W-C Chiang","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ijcard.2016.07.260","article-title":"Microparticles and blood cells induce procoagulant activity via phosphatidylserine exposure in NSTEMI patients following stent implantation","volume":"223","author":"L Wang","year":"2016","journal-title":"Int J Cardiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0168771","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T09:24:57Z","timestamp":1540200297000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0168771"}},"subtitle":[],"editor":[{"given":"Tom","family":"Coenye","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,12,28]]},"references-count":42,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2016,12,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0168771","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,28]]}}}