{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:11:49Z","timestamp":1790673109132,"version":"4.1.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2017,1,3]],"date-time":"2017-01-03T00:00:00Z","timestamp":1483401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI11822"],"award-info":[{"award-number":["AI11822"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Howard Hughes Medical Institute (US)"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0169180","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T22:11:05Z","timestamp":1483999865000},"page":"e0169180","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":40,"title":["The Phage Lysin PlySs2 Decolonizes Streptococcus suis from Murine Intranasal Mucosa"],"prefix":"10.1371","volume":"12","author":[{"given":"Daniel B.","family":"Gilmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan E.","family":"Schmitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mya","family":"Thandar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad W.","family":"Euler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent A.","family":"Fischetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,1,3]]},"reference":[{"issue":"1\u20133","key":"ref1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.vetmic.2005.12.013","article-title":"Virulence-associated gene profiling of Streptococcus suis isolates by PCR","volume":"115","author":"LM Silva","year":"2006","journal-title":"Veterinary microbiology"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.micpath.2008.04.009","article-title":"Comparative proteome analysis of secreted proteins of Streptococcus suis serotype 9 isolates from diseased and healthy pigs","volume":"45","author":"Z Wu","year":"2008","journal-title":"Microbial pathogenesis"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2217\/fmb.10.2","article-title":"Streptococcus suis: a new emerging or an old neglected zoonotic pathogen?","volume":"5","author":"M Gottschalk","year":"2010","journal-title":"Future Microbiol"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"e187","DOI":"10.1371\/journal.pmed.0030187","article-title":"Invasive disease and toxic shock due to zoonotic Streptococcus suis: an emerging infection in the East?","volume":"3","author":"S Sriskandan","year":"2006","journal-title":"PLoS Med"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"835","DOI":"10.3201\/eid1705.100754","article-title":"Genotypic profile of Streptococcus suis serotype 2 and clinical features of infection in humans, Thailand","volume":"17","author":"A Kerdsin","year":"2011","journal-title":"Emerg Infect Dis"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"e61339","DOI":"10.1371\/journal.pone.0061339","article-title":"Effect of spatial separation of pigs on spread of Streptococcus suis serotype 9","volume":"8","author":"N Dekker","year":"2013","journal-title":"PloS one"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/clinids\/10.1.131","article-title":"Meningitis caused by Streptococcus suis in humans","volume":"10","author":"JP Arends","year":"1988","journal-title":"Rev Infect Dis"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1699-0463.1968.tb03456.x","article-title":"Group R streptococci pathogenic for man. Two cases of meningitis and one fatal case of sepsis","volume":"74","author":"B Perch","year":"1968","journal-title":"Acta pathologica et microbiologica Scandinavica"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"914","DOI":"10.3201\/eid1206.051194","article-title":"Human Streptococcus suis outbreak, Sichuan, China","volume":"12","author":"H Yu","year":"2006","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1093\/clinids\/13.6.1251","article-title":"A case of human endocarditis due to Streptococcus suis in North America","volume":"13","author":"S Trottier","year":"1991","journal-title":"Reviews of infectious diseases"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1177\/104063879200400209","article-title":"Antimicrobial resistance patterns and plasmid profiles of Streptococcus suis isolates","volume":"4","author":"M Cantin","year":"1992","journal-title":"J Vet Diagn Invest"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1017\/S1466252313000029","article-title":"Antimicrobial resistance and prudent drug use for Streptococcus suis","volume":"14","author":"NP Varela","year":"2013","journal-title":"Animal health research reviews \/ Conference of Research Workers in Animal Diseases"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1146\/annurev.micro.54.1.799","article-title":"Holins: the protein clocks of bacteriophage infections","volume":"54","author":"IN Wang","year":"2000","journal-title":"Annu Rev Microbiol"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.mib.2008.09.012","article-title":"Bacteriophage lysins as effective antibacterials","volume":"11","author":"VA Fischetti","year":"2008","journal-title":"Curr Opin Microbiol"},{"issue":"7","key":"ref15","doi-asserted-by":"crossref","first-page":"4107","DOI":"10.1073\/pnas.061038398","article-title":"Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme","volume":"98","author":"D Nelson","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5549","key":"ref16","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1126\/science.1066869","article-title":"Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase","volume":"294","author":"JM Loeffler","year":"2001","journal-title":"Science"},{"issue":"10","key":"ref17","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.tim.2005.08.007","article-title":"Bacteriophage lytic enzymes: novel anti-infectives","volume":"13","author":"VA Fischetti","year":"2005","journal-title":"Trends Microbiol"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1128\/AAC.01625-09","article-title":"Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureus","volume":"54","author":"A Daniel","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/AAC.49.1.111-117.2005","article-title":"Removal of group B streptococci colonizing the vagina and oropharynx of mice with a bacteriophage lytic enzyme","volume":"49","author":"Q Cheng","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"ref20","doi-asserted-by":"crossref","first-page":"6104","DOI":"10.1128\/IAI.71.10.6104-6108.2003","article-title":"Identification of an inducible bacteriophage in a virulent strain of Streptococcus suis serotype 2","volume":"71","author":"J Harel","year":"2003","journal-title":"Infection and immunity"},{"issue":"3\u20134","key":"ref21","first-page":"340","article-title":"Isolation and identification of a bacteriophage capable of infecting Streptococcus suis type 2 strains","volume":"132","author":"YL Ma","year":"2008","journal-title":"Veterinary microbiology"},{"issue":"6","key":"ref22","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s00284-009-9379-x","article-title":"Purified recombinant phage lysin LySMP: an extensive spectrum of lytic activity for swine streptococci","volume":"58","author":"Y Wang","year":"2009","journal-title":"Current microbiology"},{"issue":"23","key":"ref23","doi-asserted-by":"crossref","first-page":"8272","DOI":"10.1128\/AEM.05151-11","article-title":"Application of a bacteriophage lysin to disrupt biofilms formed by the animal pathogen Streptococcus suis","volume":"77","author":"X Meng","year":"2011","journal-title":"Appl Environ Microbiol"},{"issue":"21","key":"ref24","doi-asserted-by":"crossref","first-page":"7377","DOI":"10.1128\/AEM.02300-15","article-title":"Prophage lysin Ply30 protects mice from Streptococcus suis and Streptococcus equi subsp. zooepidemicus infections","volume":"81","author":"F Tang","year":"2015","journal-title":"Appl Environ Microbiol"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"2743","DOI":"10.1128\/AAC.02526-12","article-title":"Novel bacteriophage lysin with broad lytic activity protects against mixed infection by Streptococcus pyogenes and methicillin-resistant Staphylococcus aureus","volume":"57","author":"DB Gilmer","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"I Wiegand","year":"2008","journal-title":"Nat Protoc"},{"issue":"8","key":"ref27","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.1128\/AAC.49.8.3187-3191.2005","article-title":"In vitro and in vivo evaluations of the activities of lauric acid monoester formulations against Staphylococcus aureus","volume":"49","author":"MS Rouse","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1128\/AAC.00890-10","article-title":"A Novel Chimeric Lysin Shows Superiority to Mupirocin for Skin Decolonization of Methicillin-Resistant and -Sensitive Staphylococcus aureus Strains","volume":"55","author":"M Pastagia","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"issue":"Pt 9","key":"ref29","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1099\/jmm.0.043885-0","article-title":"A novel intranasal mouse model for mucosal colonization by Streptococcus suis serotype 2","volume":"61","author":"M Seitz","year":"2012","journal-title":"Journal of medical microbiology"},{"issue":"8","key":"ref30","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1086\/521305","article-title":"Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage phi MR11","volume":"196","author":"M Rashel","year":"2007","journal-title":"J Infect Dis"},{"issue":"6900","key":"ref31","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1038\/nature01026","article-title":"A bacteriolytic agent that detects and kills Bacillus anthracis","volume":"418","author":"R Schuch","year":"2002","journal-title":"Nature"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/BF02214715","article-title":"Studies of Streptococcus suis type 2 infection in pigs","volume":"8","author":"FA Clifton-Hadley","year":"1984","journal-title":"Veterinary research communications"},{"issue":"15","key":"ref33","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1136\/vr.124.15.391","article-title":"Prevalence of Streptococcus suis types 1 and 2 in domestic pigs in Australia and New Zealand","volume":"124","author":"ID Robertson","year":"1989","journal-title":"The Veterinary record"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1017\/S0950268800048743","article-title":"A longitudinal study of natural infection of piglets with Streptococcus suis types 1 and 2","volume":"107","author":"ID Robertson","year":"1991","journal-title":"Epidemiology and infection"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1177\/104063879600800110","article-title":"Evaluation of antibiotics for the elimination of the tonsillar carrier state of Streptococcus suis in pigs","volume":"8","author":"SF Amass","year":"1996","journal-title":"Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc."},{"issue":"1","key":"ref36","first-page":"21","article-title":"Colonization of suckling pigs by Streptococcus suis with particular reference to pathogenic serotype 2 strains","volume":"62","author":"M Torremorell","year":"1998","journal-title":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire"},{"issue":"1\u20132","key":"ref37","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.vetmic.2003.09.018","article-title":"Epidemiology of Streptococcus suis serotype 5 infection in a pig herd with and without clinical disease","volume":"97","author":"G Cloutier","year":"2003","journal-title":"Veterinary microbiology"},{"issue":"18","key":"ref38","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1136\/vr.160.18.619","article-title":"Elimination of strains of Streptococcus suis serotype 2 from the tonsils of carrier sows by combined medication and vaccination","volume":"160","author":"B Swildens","year":"2007","journal-title":"The Veterinary record"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1128\/AAC.49.3.1225-1228.2005","article-title":"Synergistic killing of Streptococcus pneumoniae with the bacteriophage lytic enzyme Cpl-1 and penicillin or gentamicin depends on the level of penicillin resistance","volume":"49","author":"S Djurkovic","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"ref40","article-title":"Combination Therapy With Lysin CF-301 and Antibiotic Is Superior to Antibiotic Alone for Treating Methicillin-Resistant Staphylococcus aureus-Induced Murine Bacteremia","author":"R Schuch","year":"2013","journal-title":"J Infect Dis"},{"issue":"3","key":"ref41","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1128\/JB.137.3.1158-1164.1979","article-title":"Relationship between lysostaphin endopeptidase production and cell wall composition in Staphylococcus staphylolyticus","volume":"137","author":"JM Robinson","year":"1979","journal-title":"J Bacteriol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0169180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T19:33:52Z","timestamp":1601321632000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0169180"}},"subtitle":[],"editor":[{"given":"Jose","family":"Melo-Cristino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,1,3]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,1,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0169180","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,3]]}}}