{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:54:08Z","timestamp":1777611248257,"version":"3.51.4"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T00:00:00Z","timestamp":1484265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["01RO1HL126874"],"award-info":[{"award-number":["01RO1HL126874"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["01RO1HL128398"],"award-info":[{"award-number":["01RO1HL128398"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0169537","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T18:36:05Z","timestamp":1484332565000},"page":"e0169537","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mechanism of Collaborative Enhancement of Binding of Paired Antibodies to Distinct Epitopes of Platelet Endothelial Cell Adhesion Molecule-1"],"prefix":"10.1371","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8065-3551","authenticated-orcid":true,"given":"Raisa","family":"Kiseleva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin F.","family":"Greineder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos H.","family":"Villa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth D.","family":"Hood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir V.","family":"Shuvaev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann-Marie","family":"Chacko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valsamma","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horace M.","family":"DeLisser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir R.","family":"Muzykantov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,1,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jconrel.2012.08.031","article-title":"Antioxidant protection by PECAM-targeted delivery of a novel NADPH-oxidase inhibitor to the endothelium in vitro and in vivo","volume":"163","author":"ED Hood","year":"2012","journal-title":"J Control Release"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1152\/ajprenal.00400.2011","article-title":"inflammation heterogeneity of VCAM-1 protein expression in acute MicroRNA-126 contributes to renal microvascular MicroRNA-126 contributes to renal microvascular heterogeneity of VCAM-1 protein expression in acute inflammation","volume":"302","author":"TJ Rabelink","year":"2012","journal-title":"Am J Physiol Ren Physiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/iub.474","article-title":"Vascular targeting of antithrombotic agents","volume":"63","author":"R Carnemolla","year":"2011","journal-title":"IUBMB Life"},{"key":"ref4","first-page":"108","article-title":"Targeted In Vivo Labeling of Receptors for Vascular Endothelial Growth Factor","author":"E Lu","year":"2003","journal-title":"Circulation"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"14710","DOI":"10.1073\/pnas.1408556111","article-title":"ICAM-1 as a molecular target for triple negative breast cancer","volume":"111","author":"P Guo","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaf7374","article-title":"P-selectin is a nanotherapeutic delivery target in the tumor microenvironment","volume":"8","author":"Y Shamay","year":"2016","journal-title":"Sci Transl Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.1096\/fj.15-271213","article-title":"Dual targeting of therapeutics to endothelial cells: Collaborative enhancement of delivery and effect","volume":"29","author":"CF Greineder","year":"2015","journal-title":"FASEB J"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1096\/fj.10-169789","article-title":"PECAM-targeted delivery of SOD inhibits endothelial inflammatory response","volume":"25","author":"V Shuvaev V","year":"2011","journal-title":"FASEB J"},{"key":"ref9","article-title":"Binding, Transcytosis and Biodistribution of Anti-PECAM-1 Iron Oxide Nanoparticles for Brain-Targeted Delivery","author":"M Dan","year":"2013","journal-title":"PLoS One"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1182\/blood-2015-07-660092","article-title":"Structural basis for PECAM-1 homophilic binding","volume":"127","author":"C Paddock","year":"2016","journal-title":"Blood"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e34958","DOI":"10.1371\/journal.pone.0034958","article-title":"Collaborative enhancement of antibody binding to distinct pecam-1 epitopes modulates endothelial targeting","volume":"7","author":"AM Chacko","year":"2012","journal-title":"PLoS One"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"11090","DOI":"10.1074\/jbc.271.19.11090","article-title":"Individually distinct Ig homology domains in PECAM-1 regulate homophilic binding and modulate receptor affinity","volume":"271","author":"QH Sun","year":"1996","journal-title":"J Biol Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1124\/jpet.105.098970","article-title":"Endothelial targeting of high-affinity multivalent polymer nanocarriers directed to intercellular adhesion molecule 1","volume":"317","author":"S Muro","year":"2006","journal-title":"J Pharmacol Exp Ther"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"4191","DOI":"10.1182\/blood-2005-05-2002","article-title":"Endothelial targeting of a recombinant construct fusing a PECAM-1 single-chain variable antibody fragment (scFv) with prourokinase facilitates prophylactic thrombolysis in the pulmonary vasculature","volume":"106","author":"B-S Ding","year":"2005","journal-title":"Blood"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1242\/jcs.00367","article-title":"A novel endocytic pathway induced by clustering endothelial ICAM-1 or PECAM-1","volume":"116","author":"S Muro","year":"2003","journal-title":"J Cell Sci"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"18561","DOI":"10.1074\/jbc.271.31.18561","article-title":"Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) Homophilic Adhesion Is Mediated by Immunoglobulin-like Domains 1 and 2 and Depends on the Cytoplasmic Domain and the Level of Surface Expression","volume":"271","author":"J Sun","year":"1996","journal-title":"J Biol Chem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.4049\/jimmunol.153.10.4673","article-title":"Signals from platelet\/endothelial cell adhesion molecule enhance the adhesive activity of the very late antigen-4 integrin of human CD34+ hemopoietic progenitor cells","volume":"153","author":"DI Leavesley","year":"1994","journal-title":"J Immunol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1083\/jcb.110.4.1227","article-title":"EndoCAM: A novel endothelial cell-cell adhesion molecule","volume":"110","author":"SM Albelda","year":"1990","journal-title":"J Cell Biol"},{"key":"ref19","first-page":"2055","article-title":"Use of recombinant adenovirus to transfer the herpes simplex virus thymidine kinase (HSVtk) gene to thoracic neoplasms: an effective in vitro drug sensitization system","volume":"54","author":"WR Smythe","year":"1994","journal-title":"Cancer Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1242\/dev.120.9.2539","article-title":"Platelet endothelial cell adhesion molecule-1 (PECAM-1\/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development","volume":"120","author":"HS Baldwin","year":"1994","journal-title":"Development"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"20555","DOI":"10.1074\/jbc.272.33.20555","article-title":"Residues on Both Faces of the First Immunoglobulin Fold Contribute to Homophilic Binding Sites of PECAM-1\/CD31","volume":"272","author":"JP Newton","year":"1997","journal-title":"J Biol Chem"},{"key":"ref22","article-title":"Fractionation of Cells","author":"B Alberts","year":"2002","journal-title":"Molecular Biology of the Cell"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3109\/15419069509081277","article-title":"Localization of multiple functional domains on human PECAM-1 (CD31) by monoclonal antibody epitope mapping","volume":"3","author":"HC Yan","year":"1995","journal-title":"Cell Adhes Commun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"6785","DOI":"10.1021\/nn505672x","article-title":"Collaborative Enhancement of Endothelial Targeting of Nanocarriers by Modulating Platelet-Endothelial Cell Adhesion Molecule-1\/CD31 Epitope Engagement","volume":"9","author":"AM Chacko","year":"2015","journal-title":"ACS Nano"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/intimm\/dxg036","article-title":"Evidence of allosteric conformational changes in the antibody constant region upon antigen binding","volume":"15","author":"M Oda","year":"2003","journal-title":"Int Immunol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.molimm.2004.07.004","article-title":"Conformational changes in the antibody constant domains upon hapten-binding","volume":"42","author":"T Sagawa","year":"2005","journal-title":"Mol Immunol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1096\/fasebj.9.1.7821765","article-title":"Structural features of the reactions between antibodies and protein antigens","volume":"9","author":"BC Braden","year":"1995","journal-title":"FASEB J"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1002\/jmr.1092","article-title":"A new paradigm in molecular recognition? Specific antibody binding to membrane-inserted HIV-1 epitopes","author":"JL Nieva","year":"2011","journal-title":"Journal of molecular recognition\u202f: JMR"},{"key":"ref29","article-title":"Immunobiology of HLA","author":"B Dupont","year":"1989"},{"key":"ref30","first-page":"2567","article-title":"Selective targeting of angiogenic tumor vasculature by vascular endothelial-cadherin antibody inhibits tumor growth without affecting vascular permeability","volume":"62","author":"F Liao","year":"2002","journal-title":"Cancer Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"L794","DOI":"10.1152\/ajplung.2000.278.4.L794","article-title":"Immunotargeting of glucose oxidase to endothelium in vivo causes oxidative vascular injury in the lungs","volume":"278","author":"M Christofidou-Solomidou","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"L504","DOI":"10.1152\/ajplung.2000.278.3.L504","article-title":"Targeted gene delivery to pulmonary endothelium by anti-PECAM antibody","volume":"278","author":"S Li","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"ref33","first-page":"323","article-title":"Platelet-Endothelial Cell Adhesion Molecule-1-Directed Endothelial Targeting of Superoxide Dismutase Alleviates Oxidative Stress Caused by Either Extracellular or Intracellular Superoxide","author":"V V Shuvaev","year":"2007","journal-title":"J Pharmacol Exp Ther"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1124\/jpet.109.156877","article-title":"Targeted detoxification of selected reactive oxygen species in the vascular endothelium","volume":"331","author":"V Shuvaev V","year":"2009","journal-title":"J Pharmacol Exp Ther"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0169537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T18:59:12Z","timestamp":1692644352000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0169537"}},"subtitle":[],"editor":[{"given":"Nicholas J.","family":"Mantis","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,1,13]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,1,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0169537","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,13]]}}}