{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:28:58Z","timestamp":1791325738451,"version":"4.3.1"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T00:00:00Z","timestamp":1484611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANPCyT MINCyT","award":["PICT 2013 1937"],"award-info":[{"award-number":["PICT 2013 1937"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0169936","type":"journal-article","created":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T19:10:06Z","timestamp":1484680206000},"page":"e0169936","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms"],"prefix":"10.1371","volume":"12","author":[{"given":"Juan","family":"Gorgojo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Scharrig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo M.","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric T.","family":"Harvill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eugenia","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,1,17]]},"reference":[{"key":"ref1","first-page":"255","article-title":"<italic>Bordetella parapertussis<\/italic> infections","volume":"89","author":"P Mastrantonio","year":"1997","journal-title":"Dev Biol Stand"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1136\/adc.88.8.684","article-title":"Clinical and epidemiological picture of <italic>B pertussis<\/italic> and <italic>B parapertussis<\/italic> infections after introduction of acellular pertussis vaccines","volume":"88","author":"JG Liese","year":"2003","journal-title":"Arch Dis Child"},{"issue":"15\u201316","key":"ref3","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1016\/j.vaccine.2003.11.005","article-title":"Efficacies of whole cell and acellular pertussis vaccines against <italic>Bordetella parapertussis<\/italic> in a mouse model","volume":"22","author":"S David","year":"2004","journal-title":"Vaccine"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1086\/515154","article-title":"Evidence of efficacy of the Lederle\/Takeda acellular pertussis component diphtheria and tetanus toxoids and pertussis vaccine but not the Lederle whole-cell component diphtheria and tetanus toxoids and pertussis vaccine against <italic>Bordetella parapertussis<\/italic> infection","volume":"28","author":"U Heininger","year":"1999","journal-title":"Clin Infect Dis"},{"issue":"9","key":"ref5","doi-asserted-by":"crossref","first-page":"e6989","DOI":"10.1371\/journal.pone.0006989","article-title":"O antigen allows <italic>B<\/italic>. <italic>parapertussis<\/italic> to evade <italic>B<\/italic>. <italic>pertussis<\/italic> vaccine-induced immunity by blocking binding and functions of cross-reactive antibodies","volume":"4","author":"X Zhang","year":"2009","journal-title":"PLoS One"},{"issue":"12","key":"ref6","doi-asserted-by":"crossref","first-page":"5175","DOI":"10.1128\/IAI.02553-14","article-title":"<italic>Bordetella parapertussis<\/italic> Survives inside Human Macrophages in Lipid Raft-Enriched Phagosomes","volume":"82","author":"J Gorgojo","year":"2014","journal-title":"Infect Immun"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"4309","DOI":"10.1128\/IAI.00662-12","article-title":"<italic>Bordetella parapertussis<\/italic> survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen","volume":"80","author":"J Gorgojo","year":"2012","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1600-065X.2007.00550.x","article-title":"How human neutrophils kill and degrade microbes: an integrated view","volume":"219","author":"WM Nauseef","year":"2007","journal-title":"Immunol Rev"},{"issue":"5663","key":"ref9","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"V Brinkmann","year":"2004","journal-title":"Science"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/1710-1492-2-3-98","article-title":"Mechanisms of degranulation in neutrophils","volume":"2","author":"P Lacy","year":"2006","journal-title":"Allergy Asthma Clin Immunol"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1002\/(SICI)1097-0169(1997)38:3<215::AID-CM1>3.0.CO;2-4","article-title":"Video-rate dynamics of exocytotic events associated with phagocytosis in neutrophils","volume":"38","author":"E Suzaki","year":"1997","journal-title":"Cell Motil Cytoskeleton"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"409","DOI":"10.4049\/jimmunol.159.1.409","article-title":"Fc receptor-triggered insertion of secretory granules into the plasma membrane of human neutrophils: selective retrieval during phagocytosis","volume":"159","author":"H Tapper","year":"1997","journal-title":"J Immunol"},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1038\/nm.2847","article-title":"Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo","volume":"18","author":"BG Yipp","year":"2012","journal-title":"Nat Med"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.chom.2012.06.011","article-title":"Intravascular neutrophil extracellular traps capture bacteria from the bloodstream during sepsis","volume":"12","author":"B McDonald","year":"2012","journal-title":"Cell Host Microbe"},{"issue":"10","key":"ref15","doi-asserted-by":"crossref","first-page":"e1000639","DOI":"10.1371\/journal.ppat.1000639","article-title":"Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against <italic>Candida albicans<\/italic>","volume":"5","author":"CF Urban","year":"2009","journal-title":"PLoS Pathog"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"e1000873","DOI":"10.1371\/journal.ppat.1000873","article-title":"Production of extracellular traps against <italic>Aspergillus fumigatus<\/italic> in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA","volume":"6","author":"S Bruns","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref17","first-page":"382","article-title":"Neutrophils cast extracellular traps in response to protozoan parasites","volume":"3","author":"DS Abi Abdallah","year":"2012","journal-title":"Front Immunol"},{"issue":"16","key":"ref18","doi-asserted-by":"crossref","first-page":"6748","DOI":"10.1073\/pnas.0900226106","article-title":"<italic>Leishmania amazonensis<\/italic> promastigotes induce and are killed by neutrophil extracellular traps","volume":"106","author":"AB Guimaraes-Costa","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.ajpath.2011.03.013","article-title":"Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis","volume":"179","author":"T Narasaraju","year":"2011","journal-title":"Am J Pathol"},{"key":"ref20","first-page":"365","article-title":"A NET Outcome","volume":"3","author":"T Lu","year":"2012","journal-title":"Front Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2013.00001","article-title":"NET balancing: a problem in inflammatory lung diseases","volume":"4","author":"OZ Cheng","year":"2013","journal-title":"Front Immunol"},{"issue":"11","key":"ref22","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.it.2009.07.011","article-title":"NETs: a new strategy for using old weapons","volume":"30","author":"V Papayannopoulos","year":"2009","journal-title":"Trends Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"929743","DOI":"10.1155\/2012\/929743","article-title":"ETosis: A Microbicidal Mechanism beyond Cell Death","volume":"2012","author":"AB Guimaraes-Costa","year":"2012","journal-title":"J Parasitol Res"},{"issue":"8","key":"ref24","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nrmicro1710","article-title":"Beneficial suicide: why neutrophils die to make NETs","volume":"5","author":"V Brinkmann","year":"2007","journal-title":"Nat Rev Microbiol"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1083\/jcb.201006052","article-title":"Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps","volume":"191","author":"V Papayannopoulos","year":"2010","journal-title":"J Cell Biol"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/cr.2010.150","article-title":"Neutrophil extracellular trap cell death requires both autophagy and superoxide generation","volume":"21","author":"Q Remijsen","year":"2011","journal-title":"Cell Res"},{"issue":"10","key":"ref27","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1164\/ajrccm.185.10.1130","article-title":"Dynamic changes in the expression of neutrophil extracellular traps in acute respiratory infections","volume":"185","author":"T Hirose","year":"2012","journal-title":"Am J Respir Crit Care Med"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.4049\/jimmunol.1004201","article-title":"Innate immune collectin surfactant protein D simultaneously binds both neutrophil extracellular traps and carbohydrate ligands and promotes bacterial trapping","volume":"187","author":"DN Douda","year":"2011","journal-title":"J Immunol"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.yexmp.2012.03.003","article-title":"Doxycycline treatment attenuates acute lung injury in mice infected with virulent influenza H3N2 virus: involvement of matrix metalloproteinases","volume":"92","author":"HH Ng","year":"2012","journal-title":"Exp Mol Pathol"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1046\/j.1365-2958.1996.354877.x","article-title":"The identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in <italic>Bordetella pertussis<\/italic>","volume":"19","author":"A Allen","year":"1996","journal-title":"Mol Microbiol"},{"issue":"8","key":"ref31","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1128\/IAI.67.8.3763-3767.1999","article-title":"Genetic basis for lipopolysaccharide O antigen biosynthesis in bordetellae","volume":"67","author":"A Preston","year":"1999","journal-title":"Infect Immun"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/j.1574-695X.2006.00136.x","article-title":"Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of <italic>Bordetella pertussis<\/italic> to epithelial alveolar cells","volume":"48","author":"ML Perez Vidakovics","year":"2006","journal-title":"FEMS Immunol Med Microbiol"},{"issue":"25","key":"ref33","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1182\/blood-2009-08-240713","article-title":"Fetal calf serum contains heat-stable nucleases that degrade neutrophil extracellular traps","volume":"114","author":"M von Kockritz-Blickwede","year":"2009","journal-title":"Blood"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1182\/blood.V78.4.885.885","article-title":"Neutrophils express the high affinity receptor for IgG (Fc gamma RI, CD64) after in vivo application of recombinant human granulocyte colony-stimulating factor","volume":"78","author":"R Repp","year":"1991","journal-title":"Blood"},{"issue":"10","key":"ref35","doi-asserted-by":"crossref","first-page":"4972","DOI":"10.1128\/IAI.00763-07","article-title":"The O antigen enables <italic>Bordetella parapertussis<\/italic> to avoid <italic>Bordetella pertussis<\/italic>-induced immunity","volume":"75","author":"DN Wolfe","year":"2007","journal-title":"Infect Immun"},{"issue":"11","key":"ref36","doi-asserted-by":"crossref","first-page":"6545","DOI":"10.4049\/jimmunol.167.11.6545","article-title":"Fc receptor-mediated immunity against <italic>Bordetella pertussis<\/italic>","volume":"167","author":"ME Rodriguez","year":"2001","journal-title":"J Immunol"},{"issue":"6","key":"ref37","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1086\/319266","article-title":"Targeting to Fcgamma receptors, but not CR3 (CD11b\/CD18), increases clearance of <italic>Bordetella pertussis<\/italic>","volume":"183","author":"SM Hellwig","year":"2001","journal-title":"J Infect Dis"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1574-695X.2009.00557.x","article-title":"Cholesterol-dependent attachment of human respiratory cells by <italic>Bordetella pertussis<\/italic>","volume":"56","author":"Y Lamberti","year":"2009","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"391","DOI":"10.3389\/fimmu.2012.00391","article-title":"Stable Redox-Cycling Nitroxide Tempol Inhibits NET Formation","volume":"3","author":"A Hosseinzadeh","year":"2012","journal-title":"Front Immunol"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1128\/IAI.67.5.2090-2095.1999","article-title":"Epitope mapping of monoclonal antibodies against <italic>Bordetella pertussis<\/italic> adenylate cyclase toxin","volume":"67","author":"SJ Lee","year":"1999","journal-title":"Infect Immun"},{"issue":"4","key":"ref41","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.cub.2006.01.056","article-title":"An endonuclease allows <italic>Streptococcus pneumoniae<\/italic> to escape from neutrophil extracellular traps","volume":"16","author":"K Beiter","year":"2006","journal-title":"Curr Biol"},{"issue":"5","key":"ref42","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/omi.12099","article-title":"The role of phagocytosis, oxidative burst and neutrophil extracellular traps in the interaction between neutrophils and the periodontal pathogen <italic>Porphyromonas gingivalis<\/italic>","volume":"30","author":"K Jayaprakash","year":"2015","journal-title":"Mol Oral Microbiol"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/cyto.b.10003","article-title":"Investigations of phagosomes, mitochondria, and acidic granules in human neutrophils using fluorescent probes","volume":"51","author":"CF Bassoe","year":"2003","journal-title":"Cytometry B Clin Cytom"},{"issue":"Pt 2","key":"ref44","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1042\/bj3110667","article-title":"The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils","volume":"311","author":"C Dahlgren","year":"1995","journal-title":"Biochem J"},{"issue":"12","key":"ref45","doi-asserted-by":"crossref","first-page":"5256","DOI":"10.1128\/IAI.02487-14","article-title":"Cyclic AMP-mediated suppression of neutrophil extracellular trap formation and apoptosis by the <italic>Bordetella pertussis<\/italic> adenylate cyclase toxin","volume":"82","author":"JC Eby","year":"2014","journal-title":"Infect Immun"},{"issue":"Pt 2","key":"ref46","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1099\/mic.0.072199-0","article-title":"<italic>Streptococcus suis<\/italic> DNase SsnA contributes to degradation of neutrophil extracellular traps (NETs) and evasion of NET-mediated antimicrobial activity","volume":"160","author":"N de Buhr","year":"2014","journal-title":"Microbiology"},{"issue":"6","key":"ref47","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1159\/000319909","article-title":"Nuclease expression by <italic>Staphylococcus aureus<\/italic> facilitates escape from neutrophil extracellular traps","volume":"2","author":"ET Berends","year":"2010","journal-title":"J Innate Immun"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3389\/fmicb.2016.00402","article-title":"Interaction of Bacterial Exotoxins with Neutrophil Extracellular Traps: Impact for the Infected Host","volume":"7","author":"M von Kockritz-Blickwede","year":"2016","journal-title":"Front Microbiol"},{"issue":"1","key":"ref49","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/35036052","article-title":"Lipid rafts and signal transduction","volume":"1","author":"K Simons","year":"2000","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"27","key":"ref50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S1462399402005392","article-title":"Lipid rafts: structure, function and role in HIV, Alzheimer&apos;s and prion diseases","volume":"4","author":"J Fantini","year":"2002","journal-title":"Expert Rev Mol Med"},{"issue":"1","key":"ref51","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1177\/0022034515609097","article-title":"Neutrophil Extracellular Traps in Periodontitis: A Web of Intrigue","volume":"95","author":"PC White","year":"2016","journal-title":"J Dent Res"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"929743","DOI":"10.1155\/2012\/929743","article-title":"ETosis: A Microbicidal Mechanism beyond Cell Death","volume":"2012","author":"AB Guimaraes-Costa","year":"2012","journal-title":"J Parasitol Res"},{"issue":"1","key":"ref53","doi-asserted-by":"crossref","first-page":"e4280","DOI":"10.1371\/journal.pone.0004280","article-title":"Inefficient Toll-like receptor-4 stimulation enables <italic>Bordetella parapertussis<\/italic> to avoid host immunity","volume":"4","author":"DN Wolfe","year":"2009","journal-title":"PLoS One"},{"issue":"12","key":"ref54","doi-asserted-by":"crossref","first-page":"8144","DOI":"10.1128\/IAI.73.12.8144-8152.2005","article-title":"Comparative toll-like receptor 4-mediated innate host defense to Bordetella infection","volume":"73","author":"PB Mann","year":"2005","journal-title":"Infect Immun"},{"issue":"5","key":"ref55","doi-asserted-by":"crossref","first-page":"e1004853","DOI":"10.1371\/journal.ppat.1004853","article-title":"<italic>Brucella abortus<\/italic> Induces the Premature Death of Human Neutrophils through the Action of Its Lipopolysaccharide","volume":"11","author":"E Barquero-Calvo","year":"2015","journal-title":"PLoS Pathog"},{"issue":"2","key":"ref56","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/nchembio.496","article-title":"Activation of the Raf-MEK-ERK pathway is required for neutrophil extracellular trap formation","volume":"7","author":"A Hakkim","year":"2011","journal-title":"Nat Chem Biol"},{"issue":"4","key":"ref57","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.4049\/jimmunol.1302286","article-title":"Probiotic <italic>Lactobacillus rhamnosus<\/italic> inhibits the formation of neutrophil extracellular traps","volume":"192","author":"L Vong","year":"2014","journal-title":"J Immunol"},{"issue":"10","key":"ref58","doi-asserted-by":"crossref","first-page":"6508","DOI":"10.1128\/IAI.73.10.6508-6513.2005","article-title":"Clearance of <italic>Bordetella parapertussis<\/italic> from the lower respiratory tract requires humoral and cellular immunity","volume":"73","author":"DN Wolfe","year":"2005","journal-title":"Infect Immun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0169936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T20:01:25Z","timestamp":1692648085000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0169936"}},"subtitle":[],"editor":[{"given":"Nades","family":"Palaniyar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,1,17]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,1,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0169936","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,17]]}}}