{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T08:30:43Z","timestamp":1792657843609,"version":"4.5.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T00:00:00Z","timestamp":1486944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K10441"],"award-info":[{"award-number":["15K10441"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0171448","type":"journal-article","created":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T14:47:25Z","timestamp":1486997245000},"page":"e0171448","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":127,"title":["The efficacy of a scaffold-free Bio 3D conduit developed from human fibroblasts on peripheral nerve regeneration in a rat sciatic nerve model"],"prefix":"10.1371","volume":"12","author":[{"given":"Hirofumi","family":"Yurie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Ikeguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Aoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukitoshi","family":"Kaizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Tajino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souichi","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Oda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisataka","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuka","family":"Akieda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manami","family":"Tsuji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,2,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"993","DOI":"10.3171\/jns.2003.98.5.0993","article-title":"Surgical outcomes of 654 ulnar nerve lesions","volume":"98","author":"DH Kim","year":"2003","journal-title":"J Neurosurg"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1053\/jhsu.2000.4165","article-title":"A 25-year perspective of peripheral nerve surgery: evolving neuroscientific concepts and clinical significance","volume":"25","author":"G Lundborg","year":"2000","journal-title":"J Hand Surg Am"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0749-0712(21)00503-5","article-title":"Techniques for nerve grafting","volume":"16","author":"H Milleisi","year":"2000","journal-title":"Hand Clin"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1002\/micr.20127","article-title":"Nerve repair by means of tubulization: Literature review and personal clinical experience comparing biological and synthetic conduits for sensory nerve repair","volume":"25","author":"B Battiston","year":"2005","journal-title":"Microsurgery"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1055\/s-0032-1333316","article-title":"Nerve repair by means of tubulization: past, rresent, future","volume":"28","author":"P Konofaos","year":"2013","journal-title":"J Reconstr Microsurg"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"965","DOI":"10.4103\/1673-5374.158362","article-title":"Biological conduits combining bone marrow mesenchymal stromal cells and extracellular matrix to treat long segment sciatic nerve defects","volume":"10","author":"Y Wang","year":"2015","journal-title":"Neural Regen Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.4103\/1673-5374.145366","article-title":"Extracellular matrix in peripheral nerve repair: how to affect neural response and nerve regeneration?","volume":"9","author":"AC De Luca","year":"2014","journal-title":"Neural Regen Res"},{"key":"ref8","first-page":"35","article-title":"Relationship between axonal regeneration and vascularity in tabulation-an experimental study in rats","volume":"23","author":"R Kakinoki","year":"1995","journal-title":"Neurosci Res"},{"key":"ref9","article-title":"Bridging a 30 mm defect in the canine ulnar nerve using vessel-containing conduits with implantation of bone marrow stromal cells","author":"Y Kaizawa","year":"2015","journal-title":"Microsurgery"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1179\/016164102101200564","article-title":"Peripheral nerve regeneration by bone marrow stromal cells","volume":"24","author":"P Cuevas","year":"2002","journal-title":"Neurol Res"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1179\/016164104225013897","article-title":"Bone marrow stromal cell implantation for peripheral nerve repair","volume":"26","author":"P Cuevas","year":"2004","journal-title":"Neurol Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/micr.20184","article-title":"Study of in vivo differentiation of rat bone marrow stromal cells into schwann cell-like cells","volume":"26","author":"X Chen","year":"2006","journal-title":"Microsurgery"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.expneurol.2006.12.004","article-title":"Transplantation of bone marrow stromal cells for peripheral nerve repair","volume":"204","author":"CJ Chen","year":"2007","journal-title":"Expl Neurol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"811","DOI":"10.3727\/000000007783465226","article-title":"Nerve regeneration promoted in a tube with vascularity containing bone marrow-derived cells","volume":"16","author":"T Yamakawa","year":"2007","journal-title":"Cell Transplant"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.brainres.2007.09.098","article-title":"Bridging small-gap peripheral nerve defects using acellular nerve allograft implanted with autologous bone marrow stromal cells in primates","volume":"1188","author":"D Wang","year":"2008","journal-title":"Brain Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1002\/micr.20818","article-title":"Isogenic venous graft supported with bone marrow stromal cells as a natural conduit for bridging a 20 mm nerve gap","volume":"30","author":"TH Nijhuis","year":"2010","journal-title":"Microsurgery"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"3779","DOI":"10.1089\/ten.tea.2010.0299","article-title":"Use of tissue-engineered nerve grafts consisting of a chitosan\/poly(lactic-co-glycolic acid)-based scaffold included with bone marrow mesenchymal cells for bridging 50-mm dog sciatic nerve gaps","volume":"16","author":"F Ding","year":"2010","journal-title":"Tissue Eng Part A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1097\/SAP.0b013e318223c2db","article-title":"Peripheral nerve defect repair with epineural tubes supported with bone marrow stromal cells: a preliminary report","volume":"67","author":"M Siemionow","year":"2011","journal-title":"Ann Plast Surg"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.jneumeth.2011.02.015","article-title":"A systematic evaluation of Schwann cell injection into acellular cold-preserved nerve grafts","volume":"197","author":"NJ Jesuraj","year":"2011","journal-title":"J Neurosci Methods"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.scr.2011.11.004","article-title":"Fate of stem cell transplants in peripheral nerves","volume":"8","author":"SK Walsh","year":"2012","journal-title":"Stem Cell Research"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0014-4886(82)90215-1","article-title":"Nerve regeneration in silicone chambers: influence of gap length and of distal stump components","volume":"76","author":"G Lundborg","year":"1982","journal-title":"Exp Neurol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1097\/00006534-198506000-00013","article-title":"Nerve regeneration through a pseudosynovial sheath in a primate model","volume":"75","author":"SE Mackinnon","year":"1985","journal-title":"Plast Reconstr Surg"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1177\/1753193411422685","article-title":"Experiences of using the bioresorbable copolyester poly(DL-lactide-\u03b5-caprolactone) nerve conduit guide Neurolac<sup>TM<\/sup> for nerve repair in peripheral nerve defects: report on a series of 28 lesions","volume":"37","author":"S Chiriac","year":"2012","journal-title":"J Hand Surg Eur Vol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1002\/jbm.a.30004","article-title":"Long-term regeneration of the rat sciatic nerve through a biodegradable poly(DL-lactide-epsilon-caprolactone) nerve guide: tissue reactions with focus on collagen III\/IV reformation","volume":"69","author":"K Jansen","year":"2004","journal-title":"J Biomed Mater Res A"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s13018-014-0098-z","article-title":"Simultaneous regeneration of full-thickness cartilage and subchondral bone defects in vivo using a three-dimensional scaffold-free autologous construct derived from high-density bone marrow-derived mesenchymal stem cells","volume":"9","author":"K Ishihara","year":"2014","journal-title":"J Orthop Surg Res"},{"key":"ref26","article-title":"Toward engineering functional organ modules by additive manufacturing","volume":"4","author":"M Francoise","year":"2012","journal-title":"Biofabrication"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e0136681","DOI":"10.1371\/journal.pone.0136681","article-title":"Scaffold-free tubular tissues created by a Bio-3D printer undergo remodeling and endothelialization when implanted in rat aortae","volume":"10","author":"H Itoh","year":"2015","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.tibtech.2007.12.009","article-title":"Engineering custom-designed osteochondral tissue grafts","volume":"26","author":"WL Grayson","year":"2008","journal-title":"Trends Biotechnol"},{"key":"ref29","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5082\/2\/2\/022001","article-title":"Tissue engineering by self-assembly and bio-printing of living cells","volume":"2","author":"K Jakab","year":"2010","journal-title":"Biofabrication"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.biomaterials.2015.01.073","article-title":"Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs","volume":"52","author":"AD Dikina","year":"2015","journal-title":"Biomaterials"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.1089\/ten.2006.12.3307","article-title":"Engineering complex tissue","volume":"12","author":"AG Mikos","year":"2006","journal-title":"Tissue Eng"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"18129","DOI":"10.1073\/pnas.0407817101","article-title":"Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds","volume":"101","author":"M Radisic","year":"2004","journal-title":"Proc. Natl Acad Sci"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/nprot.2008.183","article-title":"Electrical stimulation for cardiac tissue engineering","volume":"4","author":"N Tandon","year":"2009","journal-title":"Nat Protoc"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev.bioeng.6.040803.140037","article-title":"Tissue engineering of ligaments","volume":"6","author":"G Vunjak-Novakovic","year":"2004","journal-title":"Annu Rev Biomed Eng"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.cell.2010.08.039","article-title":"EphB signaling directs peripheral nerve regeneration through Sox-2-dependent Schwann cell sorting","volume":"143","author":"S Parrinello","year":"2010","journal-title":"Cell"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.bbr.2015.03.030","article-title":"Intermittent application of hypergravity by centrifugation attenuates disruption of rat gait induced by 2 weeks of simulated microgravity","volume":"287","author":"J Tajino","year":"2015","journal-title":"Behav Brain Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1080\/00222895.2014.1003779","article-title":"Discordance in recovery between altered locomotion and muscle atrophy induced by simulated microgravity in rats","volume":"47","author":"J Tajino","year":"2015","journal-title":"J Mot Behav"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.bbr.2013.01.017","article-title":"Kinematics of treadmill locomotion in mice raised in hypergravity","volume":"244","author":"M Bojados","year":"2013","journal-title":"Behav Brain Res"},{"key":"ref39","first-page":"17","article-title":"Storage and allogenic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model","volume":"5","author":"K Nakayama","year":"2010","journal-title":"J Brachial Plex Peripher Nerve Inj"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1002\/micr.22219","article-title":"\u201cStrategic sequences\u201d in adipose-derived stem cell nerve regeneration","volume":"34","author":"AD Widgerow","year":"2014","journal-title":"Microsurgery"},{"key":"ref41","first-page":"644","article-title":"Macrophages contribute to the maintenance of stable regenerating neurites following peripheral nerve injury","volume":"73","author":"HW Luk","year":"2003","journal-title":"Neurosci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S1474-4422(02)00040-6","article-title":"Treatment of neural injury with bone marrow stromal cells","volume":"1","author":"M Chopp","year":"2002","journal-title":"Lancet Neurol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.expneurol.2005.10.029","article-title":"Human mesenchymal stem cell subpopulations express a variety of neuro-regulatory molecules and promote neuronal cell survival and neuritogenesis","volume":"198","author":"L Crigler","year":"2006","journal-title":"Exp Neurol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.brainres.2009.01.056","article-title":"Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo","volume":"1262","author":"J Wang","year":"2009","journal-title":"Brain Res"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.neulet.2004.03.077","article-title":"Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration","volume":"362","author":"M Tohill","year":"2004","journal-title":"Neurosci Lett"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.micron.2010.05.010","article-title":"Transplantation of bone-marrow-derived cells into a nerve guide resulted in transdifferentiation into Schwann cells and effective regeneration of transected mouse sciatic nerve","volume":"41","author":"FR Pereira Lopes","year":"2010","journal-title":"Micron"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1002\/syn.21508","article-title":"Sciatic nerve repair by acellular nerve xenografts implanted with BMSCs in rat xenografts combined with BMSCs","volume":"66","author":"H Jia","year":"2012","journal-title":"Synapse"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0171448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T22:38:38Z","timestamp":1658615918000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0171448"}},"subtitle":[],"editor":[{"given":"Antal","family":"N\u00f3gr\u00e1di","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,2,13]]},"references-count":47,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0171448","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,13]]}}}