{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T13:30:45Z","timestamp":1749043845967},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T00:00:00Z","timestamp":1485993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Biotechnology Development Plan","award":["2016ZX08005-004"],"award-info":[{"award-number":["2016ZX08005-004"]}]},{"name":"Researcher Foundation of the Chinese Academy of Agricultural Sciences"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0171601","type":"journal-article","created":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T19:01:07Z","timestamp":1486062067000},"page":"e0171601","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bioactivity of a modified human Glucagon-like peptide-1"],"prefix":"10.1371","volume":"12","author":[{"given":"Fangfang","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin Yueju","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gangqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dehu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,2,2]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.2337\/dc12-s064","article-title":"Diagnosis and classification of diabetes mellitus","volume":"35","year":"2012","journal-title":"Diabetes Care"},{"key":"ref2","unstructured":"International Diabetes Federation. 2015;<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.diabetesatlas.org\/\" xlink:type=\"simple\">http:\/\/www.diabetesatlas.org\/<\/ext-link>."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"S62","DOI":"10.2337\/dc10-S062","article-title":"Diagnosis and classification of diabetes mellitus","volume":"33","year":"2010","journal-title":"Diabetes Care"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"3S","DOI":"10.1016\/S0002-9343(02)01276-7","article-title":"\u03b2-cell dysfunction and insulin resistance in type 2 diabetes: role of metabolic and genetic abnormalities","volume":"113","author":"D LeRoith","year":"2002","journal-title":"Am J Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/304368a0","article-title":"Exon duplication and divergence in the human preproglucagon gene","volume":"304","author":"GI Bell","year":"1983","journal-title":"Nature"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1530\/eje.0.1430717","article-title":"Glucagon-like peptide-1: a major regulator of pancreatic beta-cell function","volume":"143","author":"R Perfetti","year":"2000","journal-title":"Eur J Endocrinol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1152\/physrev.00034.2006","article-title":"The physiology of glucagon-like peptide 1","volume":"87","author":"JJ Holst","year":"2007","journal-title":"Physiol Rev"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1016\/S0140-6736(87)91194-9","article-title":"Glucagon-like peptide-1 7\u201336: a physiological incretin in man","volume":"330","author":"B Kreymann","year":"1987","journal-title":"The Lancet"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.1210\/endo.136.8.7628397","article-title":"Degradation of glucose-dependent insulinotropic polypeptide and truncated glucagon-like peptide 1 in vitro and in vivo by dipeptidyl peptidase IV","volume":"136","author":"TJ Kieffer","year":"1995","journal-title":"Endocrinology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3262","DOI":"10.1007\/s00125-012-2716-9","article-title":"A local glucagon-like peptide 1 (GLP-1) system in human pancreatic islets","volume":"55","author":"P Marchetti","year":"2012","journal-title":"Diabetologia"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1463-1326.2004.00430.x","article-title":"Function of a long-term, GLP-1-treated, insulin-secreting cell line is improved by preventing DPP IV-mediated degradation of GLP-1","volume":"7","author":"BD Green","year":"2005","journal-title":"Diabetes Obes Metab"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1021\/jm9909645","article-title":"Potent derivatives of glucagon-like peptide-1 with pharmacokinetic properties suitable for once daily administration","volume":"43","author":"LB Knudsen","year":"2000","journal-title":"J Med Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.2337\/diacare.28.5.1092","article-title":"Effects of exenatide (exendin-4) on glycemic control and weight over 30 weeks in metformin-treated patients with type 2 diabetes","volume":"28","author":"RA DeFronzo","year":"2005","journal-title":"Diabetes Care"},{"key":"ref14","unstructured":"Jomori T, Hayashi YJ, Makino M. GLP-1 Derivative and use thereof. International Patent Application. 2008;2008056726."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1146\/annurev-physiol-021113-170315","article-title":"Glucagon-like peptide-1: glucose homeostasis and beyond","volume":"76","author":"YM Cho","year":"2014","journal-title":"Annu Rev Physiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2174\/1874070700903010057","article-title":"A proficient approach to the production of therapeutic glucagon-like peptide-1 (GLP-1) in transgenic plants","volume":"3","author":"M Brandsma","year":"2009","journal-title":"Open Biotechnol J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.enzmictec.2015.09.001","article-title":"Linker engineering for fusion protein construction: improvement and characterization of a GLP-1 fusion protein","volume":"82","author":"Y Kong","year":"2016","journal-title":"Enzyme Microb Tech"},{"key":"ref18","first-page":"1","article-title":"Streptozotocin-induced diabetic models in mice and rats","volume":"5","author":"BL Furman","year":"2015","journal-title":"Curr Protoc Pharmacol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0014-2999(96)00795-9","article-title":"GLP-1 (9\u201336) amide is major metabolite of GLP-1 (7\u201336) amide after in vivo administration to dogs, and it acts as an antagonist on the pancreatic receptor","volume":"318","author":"LB Knudsen","year":"1996","journal-title":"Eur J Pharmacol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1210\/edrv.20.6.0385","article-title":"The glucagon-like peptides","volume":"20","author":"TJ Kieffer","year":"1999","journal-title":"Endocr Rev"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"564","DOI":"10.2174\/138920101606150407114815","article-title":"Expression of CTB-10\u00d7 rolGLP-1 in <italic>E<\/italic>. <italic>coli<\/italic> and its therapeutic effect on type 2 diabetes","volume":"16","author":"P Tu","year":"2015","journal-title":"Curr Pharm Biotech"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1085\/jgp.34.4.439","article-title":"The amino acid composition and some properties of histones","volume":"34","author":"MM Daly","year":"1951","journal-title":"J Gen Physiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1179\/acb.2003.58.6.001","article-title":"Pathophysiology of type 2 diabetes","volume":"58","author":"AJ Scheen","year":"2014","journal-title":"Acta Clin Belg"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/414799a","article-title":"Insulin signalling and the regulation of glucose and lipid metabolism","volume":"414","author":"AR Saltiel","year":"2001","journal-title":"Nature"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1385\/CBB:40:3:65","article-title":"The role of GLP-1 in the regulation of islet cell mass","volume":"40","author":"A Bulotta","year":"2004","journal-title":"Cell Biochem Biophy"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"985","DOI":"10.2174\/1389201014666131226155553","article-title":"Expression and Purification of Optimized rolGLP-1, A novel GLP-1 analog, in <italic>Escherichia Coli<\/italic> BL21 (DE3) and its good glucoregulatory effect on type 2 diabetic mice","volume":"14","author":"B Ma","year":"2013","journal-title":"Curr Pharm Biotech"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1126\/science.1388288","article-title":"Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle","volume":"257","author":"A Koff","year":"1992","journal-title":"Science"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/366701a0","article-title":"p21 is a universal inhibitor of cyclin kinases","volume":"366","author":"Y Xiong","year":"1993","journal-title":"Nature"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/0092-8674(93)90509-O","article-title":"Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death","volume":"74","author":"ZN Oltval","year":"1993","journal-title":"Cell"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"32413","DOI":"10.1074\/jbc.M506000200","article-title":"Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans","volume":"280","author":"DK Hagman","year":"2005","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1007\/s00125-005-1692-8","article-title":"Wollheim CB Suppression of Pdx-1 perturbs proinsulin processing, insulin secretion and GLP-1 signalling in INS-1 cells","volume":"48","author":"H Wang","year":"2005","journal-title":"Diabetologia"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1111\/j.2042-7158.1998.tb04008.x","article-title":"Use of protease inhibitors to improve calcitonin absorption from the small and large intestine in rats","volume":"50","author":"H Tozaki","year":"1998","journal-title":"J Pharm Pharmacol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1002\/jps.2600600909","article-title":"Influence of first\u2010pass effect on availability of drugs on oral administration","volume":"60","author":"M Gibaldi","year":"1971","journal-title":"J Pharm Sci"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1038\/nrd1695","article-title":"Protein drug stability: a formulation challenge","volume":"4","author":"S Frokjaer","year":"2005","journal-title":"Nat Rev Drug Discov"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0171601","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T06:15:59Z","timestamp":1568787359000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0171601"}},"subtitle":[],"editor":[{"given":"Michael","family":"Bader","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,2,2]]},"references-count":34,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2,2]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0171601","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,2]]}}}