{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T20:13:11Z","timestamp":1779394391026,"version":"3.53.1"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T00:00:00Z","timestamp":1486339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0171675","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T14:03:31Z","timestamp":1486389811000},"page":"e0171675","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Establishing a cost-per-result of laboratory-based, reflex Cryptococcal antigenaemia screening (CrAg) in HIV+ patients with CD4 counts less than 100 cells\/\u03bcl using a Lateral Flow Assay (LFA) at a typical busy CD4 laboratory in South Africa"],"prefix":"10.1371","volume":"12","author":[{"given":"Naseem","family":"Cassim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Schnippel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindi Marie","family":"Coetzee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7106-769X","authenticated-orcid":true,"given":"Deborah Kim","family":"Glencross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"ref1","unstructured":"National Department of Health (NDOH) (2013) The 2012 National Antenatal Sentinel HIV & Herpes Simplex Type-2 Prevalence Survey in South Africa. Pretoria, South Africa."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"232","DOI":"10.7196\/SAMJ.4752","article-title":"Routine cryptococcal antigen screening for HIV-infected patients with low CD4+ T-lymphocyte counts\u2014time to implement in South Africa?","volume":"101","author":"JN Jarvis","year":"2011","journal-title":"S Afr Med J"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1086\/368091","article-title":"The changing epidemiology of cryptococcosis: an update from population-based active surveillance in 2 large metropolitan areas, 1992\u20132000","volume":"36","author":"SA Mirza","year":"2003","journal-title":"Clin Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"914","DOI":"10.7196\/SAMJ.6228","article-title":"Phased implementation of screening for cryptococcal disease in South Africa","volume":"102","author":"NP Govender","year":"2012","journal-title":"S Afr Med J"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1086\/597262","article-title":"Screening for cryptococcal antigenemia in patients accessing an antiretroviral treatment program in South Africa","volume":"48","author":"JN Jarvis","year":"2009","journal-title":"Clin Infect Dis"},{"key":"ref6","article-title":"Rapid Advice: Diagnosis, Prevention and Management of Cryptococc al Disease in HIV -infected Ad ults, Adolescents and Children","year":"2011"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e85","DOI":"10.1097\/QAI.0b013e31824c837e","article-title":"Integrating cryptococcal antigen screening and pre-emptive treatment into routine HIV care","volume":"59","author":"R Rajasingham","year":"2012","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref8","article-title":"Guideline for the prevention, diagnosis and management of cryptococcal meningitis among HIB-infected persons: 2013 update","author":"N.P. Govender","year":"2013"},{"key":"ref9","first-page":"128","article-title":"National consolidated Guidelines for the prevention of mother-to-child transmission of HIV (PMTCT) and the management of HIV in children, adolescents and adults","year":"2015"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1371\/journal.pone.0158986","article-title":"Screening HIV-Infected Patients with Low CD4 Counts for Cryptococcal Antigenemia prior to Initiation of Antiretroviral Therapy: Cost Effectiveness of Alternative Screening Strategies in South Africa","volume":"11","author":"BA Larson","year":"2016","journal-title":"Plos One"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1517\/17530059.2012.681300","article-title":"CrAg lateral flow assay for cryptococcosis","volume":"6","author":"TR Kozel","year":"2012","journal-title":"Expert Opin Med Diagn"},{"issue":"Suppl 1","key":"ref12","doi-asserted-by":"crossref","first-page":"S40","DOI":"10.1002\/cyto.b.20384","article-title":"Large-scale affordable PanLeucogated CD4+ testing with proactive internal and external quality assessment: in support of the South African national comprehensive care, treatment and management programme for","volume":"74","author":"DK Glencross","year":"2008","journal-title":"HIV and AIDS. Cytometry B Clin Cytom"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1002\/cyto.10068","article-title":"CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration","volume":"50","author":"DK Glencross","year":"2002","journal-title":"Cytometry"},{"key":"ref14","unstructured":"National Health Laboratory Service (NHLS) (2015) Corporate Data Warehouse CCMT Report. In: (CDW) CDW, editor. Sandringham, Johannesburg: Sue Candy."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e114727","DOI":"10.1371\/journal.pone.0114727","article-title":"An integrated tiered service delivery model (ITSDM) based on local CD4 testing demands can improve turn-around times and save costs whilst ensuring accessible and scalable CD4 services across a national programme","volume":"9","author":"DK Glencross","year":"2014","journal-title":"PLoS One"},{"key":"ref16","unstructured":"Standard Bank of South Africa (SBSA) (2015) Forex closing indication rates for 23 October 2015 as at 11:00."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"713","DOI":"10.3111\/13696998.2013.784591","article-title":"Consolidated health Economics Evaluation Reporting Standards (CHEERS) statement","volume":"16","author":"D Husereau","year":"2013","journal-title":"Journal of Medical Economics"},{"key":"ref18","unstructured":"Standard Bank of South Africa (SBSA) (2016) Forex closing indication rates for 04 May 2016 as at 14:30."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.1128\/CVI.00446-12","article-title":"Comparison of four assays for the detection of cryptococcal antigen","volume":"19","author":"MJ Binnicker","year":"2012","journal-title":"Clin Vaccine Immunol"},{"key":"ref20","unstructured":"Coetzee LM, Cassim N, Moodley K, Glencross D (2014) Roadmap for implementing a national early detection programme for reflexed Cryptococcal Antigenaemia (CrAg) screening (ECAD) in national health CD4 laboratories in South Africa. 7th SA AIDS Conference. Durban, South Africa."},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Cassim N, Schnippel K, Coetzee LM, Glencross DK (2016) Estimating the cost-per-result of a national reflexed Cryptococcal antigenaemia screening program; forecasting the impact of potential HIV guideline changes.","DOI":"10.1371\/journal.pone.0182154"},{"key":"ref22","unstructured":"Cassim N, Coetzee LM, Moodley K, Schnippel K, Glencross DK (2015) Incremental costs of implementing automated EIA for cryptococcal antigenaemia detection compared to a lateral flow assay at a high-volume public-sector laboratory in South Africa. 8th International Workshop on HIV Treatment, Pathogenesis and Prevention Research in Resource-poor Settings. Lusaka, Zambia."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0171675","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T05:48:58Z","timestamp":1568785738000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0171675"}},"subtitle":[],"editor":[{"given":"Esaki M.","family":"Shankar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,2,6]]},"references-count":22,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0171675","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,6]]}}}