{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:55:13Z","timestamp":1740146113936,"version":"3.37.3"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T00:00:00Z","timestamp":1492473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["CRSII3_154455 \/ 1"],"award-info":[{"award-number":["CRSII3_154455 \/ 1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHN 451\/5-1"],"award-info":[{"award-number":["SCHN 451\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["IEF-235223-Calyx-MMFF"],"award-info":[{"award-number":["IEF-235223-Calyx-MMFF"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0175964","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T17:44:27Z","timestamp":1492537467000},"page":"e0175964","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["An organotypic slice culture to study the formation of calyx of Held synapses in-vitro"],"prefix":"10.1371","volume":"12","author":[{"given":"Elin","family":"Kronander","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Michalski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9cile","family":"Lebrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Pierre","family":"Hornung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Schneggenburger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,4,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1113\/jphysiol.1994.sp020303","article-title":"Direct patch recording from identified presynaptic terminals mediating glutamatergic EPSCs in the rat CNS, <italic>in vitro<\/italic>","volume":"479","author":"ID Forsythe","year":"1994","journal-title":"J Physiol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1113\/jphysiol.1995.sp021095","article-title":"Pre- and postsynaptic whole-cell recordings in the medial nucleus of the trapezoid body of the rat","volume":"489","author":"JG Borst","year":"1995","journal-title":"The Journal of physiology"},{"issue":"2","key":"ref3","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1007\/s00441-006-0272-7","article-title":"The calyx of Held","volume":"326","author":"R Schneggenburger","year":"2006","journal-title":"Cell and tissue research"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1146\/annurev-physiol-020911-153236","article-title":"The calyx of Held synapse: from model synapse to auditory relay","volume":"74","author":"JG Borst","year":"2012","journal-title":"Annual review of physiology"},{"issue":"14","key":"ref5","doi-asserted-by":"publisher","first-page":"5603","DOI":"10.1073\/pnas.0801395105","article-title":"Dynamic development of the calyx of Held synapse","volume":"105","author":"A Rodriguez-Contreras","year":"2008","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"ref6","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.ydbio.2009.09.043","article-title":"Deficiency of neural recognition molecule NB-2 affects the development of glutamatergic auditory pathways from the ventral cochlear nucleus to the superior olivary complex in mouse","volume":"336","author":"M Toyoshima","year":"2009","journal-title":"Developmental biology"},{"issue":"32","key":"ref7","doi-asserted-by":"publisher","first-page":"12954","DOI":"10.1523\/JNEUROSCI.1087-13.2013","article-title":"Synaptic inputs compete during rapid formation of the calyx of Held: a new model system for neural development","volume":"33","author":"PS Holcomb","year":"2013","journal-title":"The Journal of neuroscience: the official journal of the Society for Neuroscience"},{"issue":"7","key":"ref8","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1038\/nn.3414","article-title":"BMP signaling specifies the development of a large and fast CNS synapse","volume":"16","author":"L Xiao","year":"2013","journal-title":"Nature neuroscience"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.heares.2014.01.007","article-title":"Morphological and physiological development of auditory synapses","volume":"311","author":"WM Yu","year":"2014","journal-title":"Hearing research"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1152\/jn.1990.63.5.1169","article-title":"Recordings from cat trapezoid body and HRP labeling of globular bushy cell axons","volume":"63","author":"GA Spirou","year":"1990","journal-title":"Journal of neurophysiology"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1002\/cne.903040305","article-title":"Projections of physiologically characterized globular bushy cell axons from the cochlear nucleus of the cat","volume":"304","author":"PH Smith","year":"1991","journal-title":"J Comp Neurol"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/cne.901260105","article-title":"Ascending connections of the anterior ventral cochlear nucleus in the rat","volume":"126","author":"JM Harrison","year":"1966","journal-title":"J Comp Neurol"},{"issue":"2","key":"ref13","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1002\/cne.903280202","article-title":"Pre- and postnatal development of efferent connections of the cochlear nucleus in the rat","volume":"328","author":"K Kandler","year":"1993","journal-title":"The Journal of comparative neurology"},{"issue":"5","key":"ref14","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1002\/cne.21443","article-title":"Molecular guidance cues necessary for axon pathfinding from the ventral cochlear nucleus","volume":"504","author":"DM Howell","year":"2007","journal-title":"The Journal of comparative neurology"},{"issue":"20","key":"ref15","doi-asserted-by":"publisher","first-page":"5511","DOI":"10.1523\/JNEUROSCI.5525-05.2006","article-title":"Synaptogenesis of the calyx of Held: rapid onset of function and one-to-one morphological innervation","volume":"26","author":"BK Hoffpauir","year":"2006","journal-title":"The Journal of neuroscience: the official journal of the Society for Neuroscience"},{"issue":"2","key":"ref16","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1002\/cne.20918","article-title":"Branching of calyceal afferents during postnatal development in the rat auditory brainstem","volume":"496","author":"A Rodriguez-Contreras","year":"2006","journal-title":"The Journal of comparative neurology"},{"issue":"24","key":"ref17","doi-asserted-by":"publisher","first-page":"14017","DOI":"10.1073\/pnas.241333598","article-title":"Activation of the epsilon isoform of protein kinase C in the mammalian nerve terminal","volume":"98","author":"N Saitoh","year":"2001","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"Pt 9","key":"ref18","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1113\/jphysiol.2009.186676","article-title":"Regulation of Kv channel expression and neuronal excitability in rat medial nucleus of the trapezoid body maintained in organotypic culture","volume":"588","author":"H Tong","year":"2010","journal-title":"The Journal of physiology"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"8257","DOI":"10.1523\/JNEUROSCI.0550-08.2008","article-title":"Phorbol esters modulate spontaneous and Ca<sup>2+<\/sup> -evoked transmitter release via acting on both Munc13 and protein kinase C","volume":"28","author":"X Lou","year":"2008","journal-title":"J Neuroscience"},{"issue":"12","key":"ref20","doi-asserted-by":"publisher","first-page":"3600","DOI":"10.1523\/JNEUROSCI.3869-15.2016","article-title":"Reconstitution of giant mammalian synapses in culture for molecular functional and imaging studies","volume":"36","author":"D Dimitrov","year":"2016","journal-title":"J Neuroscience"},{"issue":"1","key":"ref21","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.mcn.2007.09.006","article-title":"Math5 expression and function in the central auditory system","volume":"37","author":"SM Saul","year":"2008","journal-title":"Molecular and cellular neurosciences"},{"issue":"1","key":"ref22","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.ydbio.2003.08.005","article-title":"Math5 determines the competence state of retinal ganglion cell progenitors","volume":"264","author":"Z Yang","year":"2003","journal-title":"Developmental biology"},{"issue":"7166","key":"ref23","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/nature06293","article-title":"Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system","volume":"450","author":"J Livet","year":"2007","journal-title":"Nature"},{"issue":"1","key":"ref24","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"L Madisen","year":"2010","journal-title":"Nat Neuroscience"},{"issue":"2","key":"ref25","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/0165-0270(91)90128-M","article-title":"A simple method for organotypic cultures of nervous tissue","volume":"37","author":"L Stoppini","year":"1991","journal-title":"Journal of neuroscience methods"},{"issue":"2","key":"ref26","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1002\/(SICI)1097-4695(19980205)34:2<97::AID-NEU1>3.0.CO;2-6","article-title":"Development of a topographically organized auditory network in slice culture is calcium dependent","volume":"34","author":"C Lohmann","year":"1998","journal-title":"Journal of neurobiology"},{"issue":"7","key":"ref27","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nat Methods"},{"issue":"17","key":"ref28","doi-asserted-by":"publisher","first-page":"2453","DOI":"10.1093\/bioinformatics\/btr390","article-title":"Simple Neurite Tracer: open source software for reconstruction, visualization and analysis of neuronal processes","volume":"27","author":"MH Longair","year":"2011","journal-title":"Bioinformatics"},{"issue":"2","key":"ref29","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1007\/BF00248219","article-title":"Divergent projections of physiologically characterized rat ventral cochlear nucleus neurons as shown by intra-axonal injection of horseradish peroxidase","volume":"73","author":"E Friauf","year":"1988","journal-title":"Experimental brain research"},{"issue":"1","key":"ref30","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1002\/cne.903340105","article-title":"Transient appearance of calbindin-D28k-positive neurons in the superior olivary complex of developing rats","volume":"334","author":"E Friauf","year":"1993","journal-title":"The Journal of comparative neurology"},{"issue":"6","key":"ref31","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1111\/j.1460-9568.2004.03604.x","article-title":"Developmental expression of the Ca<sup>2+<\/sup>-binding proteins calretinin and parvalbumin at the calyx of Held of rats and mice","volume":"20","author":"F Felmy","year":"2004","journal-title":"The European journal of neuroscience"},{"issue":"16","key":"ref32","doi-asserted-by":"publisher","first-page":"4408","DOI":"10.1523\/JNEUROSCI.3627-15.2016","article-title":"alpha-Synuclein mutation inhibits endocytosis at mammalian central nerve terminals","volume":"36","author":"J Xu","year":"2016","journal-title":"J Neuroscience"},{"issue":"7170","key":"ref33","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nature06308","article-title":"A dual-Ca<sup>2+<\/sup>-sensor model for neurotransmitter release in a central synapse","volume":"450","author":"J Sun","year":"2007","journal-title":"Nature"},{"issue":"4","key":"ref34","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1016\/j.neuron.2011.01.013","article-title":"Synaptotagmin increases the dynamic range of synapses by driving Ca<sup>2+<\/sup>-evoked release and by clamping a near-linear remaining Ca<sup>2+<\/sup> sensor","volume":"69","author":"O Kochubey","year":"2011","journal-title":"Neuron"},{"issue":"2","key":"ref35","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1002\/aja.1001630202","article-title":"Cochlear nuclear complex of mice","volume":"163","author":"DB Webster","year":"1982","journal-title":"The American journal of anatomy"},{"issue":"8","key":"ref36","doi-asserted-by":"publisher","first-page":"e73308","DOI":"10.1371\/journal.pone.0073308","article-title":"Morphological characterization of bushy cells and their inputs in the laboratory mouse (Mus musculus) anteroventral cochlear nucleus","volume":"8","author":"AM Lauer","year":"2013","journal-title":"PloS one"},{"issue":"3","key":"ref37","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1002\/cne.903530303","article-title":"Development of ectopic projections from the ventral cochlear nucleus to the superior olivary complex induced by neonatal ablation of the contralateral cochlea","volume":"353","author":"LM Kitzes","year":"1995","journal-title":"The Journal of comparative neurology"},{"issue":"4","key":"ref38","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1002\/cne.21002","article-title":"Deafferentation induces novel axonal projections in the auditory brainstem after hearing onset","volume":"497","author":"CY Hsieh","year":"2006","journal-title":"The Journal of comparative neurology"},{"issue":"5\u20136","key":"ref39","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/S0361-9230(03)00050-9","article-title":"Parallel auditory pathways: projection patterns of the different neuronal populations in the dorsal and ventral cochlear nuclei","volume":"60","author":"NB Cant","year":"2003","journal-title":"Brain research bulletin"},{"issue":"3","key":"ref40","doi-asserted-by":"publisher","first-page":"e1000325","DOI":"10.1371\/journal.pbio.1000325","article-title":"Genetic dissection of the function of hindbrain axonal commissures","volume":"8","author":"N Renier","year":"2010","journal-title":"PLoS biology"},{"issue":"15","key":"ref41","doi-asserted-by":"publisher","first-page":"4388","DOI":"10.1016\/j.bmcl.2008.06.052","article-title":"Structure-activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors","volume":"18","author":"GD Cuny","year":"2008","journal-title":"Bioorganic & medicinal chemistry letters"},{"issue":"1","key":"ref42","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1093\/jb\/mvp148","article-title":"Bone morphogenetic protein receptors and signal transduction","volume":"147","author":"K Miyazono","year":"2010","journal-title":"Journal of biochemistry"},{"issue":"4","key":"ref43","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/S0896-6273(02)00589-5","article-title":"wishful thinking encodes a BMP type II receptor that regulates synaptic growth in Drosophila","volume":"33","author":"H Aberle","year":"2002","journal-title":"Neuron"},{"issue":"4","key":"ref44","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/S0896-6273(02)00595-0","article-title":"The Drosophila BMP type II receptor Wishful Thinking regulates neuromuscular synapse morphology and function","volume":"33","author":"G Marques","year":"2002","journal-title":"Neuron"},{"issue":"2","key":"ref45","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/S0896-6273(03)00426-4","article-title":"The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction","volume":"39","author":"BD McCabe","year":"2003","journal-title":"Neuron"},{"issue":"7","key":"ref46","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1016\/j.cub.2006.02.061","article-title":"Postsynaptic mad signaling at the Drosophila neuromuscular junction","volume":"16","author":"V Dudu","year":"2006","journal-title":"Current biology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0175964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:06:13Z","timestamp":1588968373000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0175964"}},"subtitle":[],"editor":[{"given":"Michael A.","family":"Fox","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,4,18]]},"references-count":46,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2017,4,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0175964","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2017,4,18]]}}}