{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:04:59Z","timestamp":1768003499886,"version":"3.49.0"},"reference-count":88,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T00:00:00Z","timestamp":1496707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 1093"],"award-info":[{"award-number":["SFB 1093"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 1093"],"award-info":[{"award-number":["SFB 1093"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0178260","type":"journal-article","created":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T17:35:27Z","timestamp":1496770527000},"page":"e0178260","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Delivery of the autofluorescent protein R-phycoerythrin by calcium phosphate nanoparticles into four different eukaryotic cell lines (HeLa, HEK293T, MG-63, MC3T3): Highly efficient, but leading to endolysosomal proteolysis in HeLa and MC3T3 cells"],"prefix":"10.1371","volume":"12","author":[{"given":"Mathis","family":"Kopp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Rotan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chrisovalantis","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Schulze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemmo","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1641-7068","authenticated-orcid":true,"given":"Matthias","family":"Epple","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"7264","DOI":"10.1158\/1078-0432.CCR-07-2184","article-title":"The challenge of drugging undruggable targets in cancer: Lessons learned from targeting BCL-2 family members","volume":"13","author":"GL Verdine","year":"2007","journal-title":"Clin Cancer Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1002\/anie.200802092","article-title":"RNA Interference: From basic research to therapeutic applications","volume":"48","author":"J Kurreck","year":"2009","journal-title":"Angew Chem Int Ed"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1021\/bc500320j","article-title":"Promises and pitfalls of intracellular delivery of proteins","volume":"25","author":"A Fu","year":"2014","journal-title":"Bioconjugate Chem"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"12320","DOI":"10.1002\/anie.201403036","article-title":"Engineered nanoparticles for drug delivery in cancer therapy","volume":"53","author":"T Sun","year":"2014","journal-title":"Angew Chem Int Ed"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"7138","DOI":"10.1016\/j.biomaterials.2012.06.068","article-title":"A review of nanocarriers for the delivery of small interfering RNA","volume":"33","author":"P Kesharwani","year":"2012","journal-title":"Biomaterials"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1021\/ar200151m","article-title":"Recent advances in nonviral vectors for gene delivery","volume":"45","author":"X Guo","year":"2012","journal-title":"Acc Chem Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1111\/j.1476-5381.2009.00288.x","article-title":"Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers","volume":"157","author":"P Midoux","year":"2009","journal-title":"Br J Pharmacol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1007\/s00018-009-0053-z","article-title":"Nanocarriers&apos; entry into the cell: Relevance to drug delivery","volume":"66","author":"H Hillaireau","year":"2009","journal-title":"Cell Mol Life Sci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1002\/anie.200703039","article-title":"Inorganic nanoparticles as carriers of nucleic acids into cells","volume":"47","author":"V Sokolova","year":"2008","journal-title":"Angew Chem Int Ed"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1002\/asia.201501434","article-title":"Emerging supramolecular therapeutic carriers based on host-guest interactions","volume":"11","author":"AA Karim","year":"2016","journal-title":"Chem Asian J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1021\/ja907887z","article-title":"Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoparticles","volume":"132","author":"P Ghosh","year":"2010","journal-title":"J Am Chem Soc"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"6667","DOI":"10.1021\/nn402753y","article-title":"Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules","volume":"7","author":"R Tang","year":"2013","journal-title":"ACS nano"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1039\/C1CS15237H","article-title":"The golden age: gold nanoparticles for biomedicine","volume":"41","author":"EC Dreaden","year":"2012","journal-title":"Chem Soc Rev"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"3280","DOI":"10.1002\/anie.200904359","article-title":"Gold nanoparticles for biology and medicine","volume":"49","author":"DA Giljohann","year":"2010","journal-title":"Angew Chem Int Ed"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"6315","DOI":"10.1021\/nn201348s","article-title":"Water-soluble superparamagnetic magnetite nanoparticles with biocompatible coating for enhanced magnetic resonance imaging","volume":"5","author":"L Xiao","year":"2011","journal-title":"ACS nano"},{"key":"ref16","first-page":"20","article-title":"Long-term prevalence of NIRF-labeled magnetic nanoparticles for the diagnostic and intraoperative imaging of inflammation","volume":"10","author":"J Domey","year":"2015","journal-title":"Nanotoxicology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.mattod.2015.07.004","article-title":"Inorganic nanoparticles for biomedicine: where materials scientists meet medical research","volume":"19","author":"JJ Giner-Casares","year":"2016","journal-title":"Mater Today"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1146\/annurev.bioeng.7.060804.100432","article-title":"Quantum dots as cellular probes","volume":"7","author":"AP Alivisatos","year":"2005","journal-title":"Annu Rev Biomed Eng"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.mattod.2015.10.008","article-title":"Calcium phosphates in biomedical applications: materials for the future?","volume":"19","author":"W Habraken","year":"2016","journal-title":"Mater Today"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1002\/jbm.b.31746","article-title":"Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents","volume":"96","author":"V Uskokovic","year":"2011","journal-title":"J Biomed Mat Res B"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1039\/b805407j","article-title":"Calcium phosphate nanoparticles in biomineralization and biomaterials","volume":"18","author":"Y Cai","year":"2008","journal-title":"J Mater Chem"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1586\/14737159.5.6.893","article-title":"Calcium-phosphate nanoparticles: second-generation nonviral vectors in gene therapy","volume":"5","author":"A Maitra","year":"2005","journal-title":"Expert Rev Mol Diagn"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1111\/gtc.12374","article-title":"Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite-collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles","volume":"21","author":"T Tenkumo","year":"2016","journal-title":"Genes Cells"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12977-016-0258-9","article-title":"Combination of nanoparticle-based therapeutic vaccination and transient ablation of regulatory T cells strongly enhances anti-viral immunity during chronic retroviral infection","volume":"13","author":"T Knuschke","year":"2016","journal-title":"Retrovirology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3834","DOI":"10.1002\/jbm.a.35527","article-title":"Calcium phosphate nanoparticles carrying BMP-7 plasmid DNA induce an osteogenic response in MC3T3-E1 pre-osteoblasts","volume":"103","author":"C Hadjicharalambous","year":"2015","journal-title":"J Biomed Mater Res A"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.jconrel.2014.06.049","article-title":"Target-specific delivery of siRNA by stabilized calcium phosphate nanoparticles using dopa-hyaluronic acid conjugate","volume":"192","author":"MS Lee","year":"2014","journal-title":"J Controlled Release"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1002\/jps.23768","article-title":"Preparation of calcium phosphate nanocapsule including deoxyribonucleic acid-polyethyleneimine-hyaluronic acid ternary complex for durable gene delivery","volume":"103","author":"T Ito","year":"2014","journal-title":"J Pharm Sci"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1166\/jbn.2013.1545","article-title":"Calcium phosphate nanoparticles for transcutaneous vaccine delivery","volume":"1","author":"P Sahdev","year":"2013","journal-title":"J Biomed Nanotechnol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/mawe.201300085","article-title":"Transport of supramolecular drugs across the cell membrane by calcium phosphate nanoparticles","volume":"44","author":"O Rotan","year":"2013","journal-title":"Mater-wiss u Werkstofftech"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.ijpharm.2013.01.014","article-title":"Stabilized calcium phosphate nano-aggregates using a dopa-chitosan conjugate for gene delivery","volume":"445","author":"K Lee","year":"2013","journal-title":"Int J Pharm"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ijpharm.2012.04.046","article-title":"Calcium phosphate embedded PLGA nanoparticles: A promising gene delivery vector with high gene loading and transfection efficiency","volume":"431","author":"J Tang","year":"2012","journal-title":"Int J Pharm"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"5788","DOI":"10.1016\/j.biomaterials.2012.04.057","article-title":"The tumor accumulation and therapeutic efficacy of doxorubicin carried in calcium phosphate-reinforced polymer nanoparticles","volume":"33","author":"KH Min","year":"2012","journal-title":"Biomaterials"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1002\/wnan.163","article-title":"Calcium phosphate-based composite nanoparticles in bioimaging and therapeutic delivery applications","volume":"4","author":"A Tabakovic","year":"2011","journal-title":"WIREs Nanomed Nanobiotechnol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"721","DOI":"10.2147\/IJN.S17096","article-title":"An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery","volume":"6","author":"Y Liu","year":"2011","journal-title":"Int J Nanomed"},{"key":"ref35","first-page":"3335","article-title":"Encapsulation of plasmid DNA in calcium phosphate nanoparticles: stem cell uptake and gene transfer efficiency","volume":"6","author":"X Cao","year":"2011","journal-title":"Int J Nanomed"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1016\/j.biomaterials.2013.10.041","article-title":"The effect of particle agglomeration on the formation of a surface-connected compartment induced by hydroxyapatite nanoparticles in human monocyte-derived macrophages","volume":"35","author":"KH Muller","year":"2014","journal-title":"Biomaterials"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e28","DOI":"10.1161\/CIRCRESAHA.108.181305","article-title":"Calcium phosphate crystals induce cell death in human vascular smooth muscle cells\u2014A potential mechanism in atherosclerotic plaque destabilization","volume":"103","author":"AE Ewence","year":"2008","journal-title":"Circ Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e97565","DOI":"10.1371\/journal.pone.0097565","article-title":"Fetuin-A and albumin alter cytotoxic effects of calcium phosphate nanoparticles on human vascular smooth muscle cells","volume":"9","author":"Y Dautova","year":"2014","journal-title":"PLoS ONE"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6794","DOI":"10.1016\/j.biomaterials.2009.08.043","article-title":"The use of size-defined DNA-functionalized calcium phosphate nanoparticles to minimise intracellular calcium disturbance during transfection","volume":"30","author":"S Neumann","year":"2009","journal-title":"Biomaterials"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1007\/s11051-012-0910-9","article-title":"Calcium phosphate nanoparticles as versatile carrier for small and large molecules across cell membranes","volume":"14","author":"V Sokolova","year":"2012","journal-title":"J Nanopart Res"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.cis.2015.01.007","article-title":"Understanding nanoparticle cellular entry: A physicochemical perspective","volume":"218","author":"CM Beddoes","year":"2015","journal-title":"Adv Coll Interface Sci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.3762\/bjnano.5.248","article-title":"Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects","volume":"5","author":"L Shang","year":"2014","journal-title":"Beilstein J Nanotechnol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.3762\/bjnano.5.174","article-title":"Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages","volume":"5","author":"DA Kuhn","year":"2014","journal-title":"Beilstein J Nanotechnol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.jconrel.2012.05.020","article-title":"On the cellular processing of non-viral nanomedicines for nucleic acid delivery: mechanisms and methods","volume":"161","author":"D Vercauteren","year":"2012","journal-title":"J Control Release"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.1039\/c2cs15309b","article-title":"Endocytosis at the nanoscale","volume":"41","author":"I Canton","year":"2012","journal-title":"Chem Soc Rev"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jconrel.2010.01.036","article-title":"Endocytosis of nanomedicines","volume":"145","author":"G Sahay","year":"2010","journal-title":"J Controlled Rel"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"7527","DOI":"10.1016\/j.actbio.2013.02.034","article-title":"Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells","volume":"9","author":"V Sokolova","year":"2013","journal-title":"Acta Biomater"},{"key":"ref48","first-page":"99","article-title":"The early endosome: a busy sorting station for proteins at the crossroads","volume":"25","author":"M Jovic","year":"2010","journal-title":"Histol Histopathol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"6605","DOI":"10.1021\/nn306032k","article-title":"Multiple internalization pathways of polyelectrolyte multilayer capsules into mammalian cells","volume":"7","author":"L Kastl","year":"2013","journal-title":"ACS nano"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.jconrel.2010.11.004","article-title":"Endosomal escape pathways for delivery of biologicals","volume":"151","author":"AK Varkouhi","year":"2011","journal-title":"J Control Release"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1007\/s11095-010-0364-7","article-title":"Barriers to non-viral vector-mediated gene delivery in the nervous system","volume":"28","author":"F P\u00e9rez-Mart\u00ednez","year":"2011","journal-title":"Pharm Res"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1021\/bc100010k","article-title":"Proton sponge trick for pH-sensitive disassembly of polyethylenimine-based siRNA delivery systems","volume":"21","author":"G Creusat","year":"2010","journal-title":"Bioconjug Chem"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1039\/c1nr00002k","article-title":"Synthetic pathways to make nanoparticles fluorescent","volume":"3","author":"V Sokolova","year":"2011","journal-title":"Nanoscale"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3762\/bjnano.8.40","article-title":"Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles","volume":"8","author":"O Rotan","year":"2017","journal-title":"Beilstein J Nanotechnol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nat Methods"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/gb-2006-7-10-r100","article-title":"CellProfiler: image analysis software for identifying and quantifying cell phenotypes","volume":"7","author":"AE Carpenter","year":"2006","journal-title":"Genome Biol"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1432-1033.1993.tb18356.x","article-title":"Refined crystal structure of phycoerythrin from Porphyridium cruentum at 0.23-nm resolution and localization of the gamma subunit","volume":"218","author":"R Ficner","year":"1993","journal-title":"Eur J Biochem"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1021\/bi00454a018","article-title":"Biliprotein light-harvesting strategies, phycoerythrin 566","volume":"29","author":"R MacColl","year":"1990","journal-title":"Biochemistry"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1021\/bi00375a033","article-title":"Kinetics of allophycocyanin&apos;s trimer-monomer equilibrium","volume":"26","author":"C Huang","year":"1987","journal-title":"Biochemistry"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"5487","DOI":"10.1016\/S0021-9258(19)41208-8","article-title":"Characterization of R-phycocyanin. Chromophore content of R-phycocyanin and C-phycoerythrin","volume":"250","author":"AN Glazer","year":"1975","journal-title":"J Biol Chem"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1111\/j.1751-1097.1987.tb07893.x","article-title":"Excitation energy transfer between sensitizing chromophores of phycocyanin 612","volume":"45","author":"K Csatorday","year":"1987","journal-title":"Photochem Photobiol"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1751-1097.1988.tb02728.x","article-title":"The development of exciton migration routes for phycocyanin 645 and allophycocyanin","volume":"47","author":"K Csatorday","year":"1988","journal-title":"Photochem Photobiol"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"166","DOI":"10.3390\/md14090166","article-title":"R-Phycoerythrin induces SGC-7901 apoptosis by arresting cell cycle at S phase","volume":"14","author":"H Tan","year":"2016","journal-title":"Marine Drugs"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"2166","DOI":"10.1039\/b810026h","article-title":"Calcium phosphate nanoparticles with adjustable dispersability and crystallinity","volume":"19","author":"H Urch","year":"2009","journal-title":"J Mater Chem"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"4029","DOI":"10.1016\/j.actbio.2011.07.010","article-title":"Quantitative determination of the composition of multi-shell calcium phosphate-oligonucleotide nanoparticles and their application for the activation of dendritic cells","volume":"7","author":"V Sokolova","year":"2011","journal-title":"Acta Biomater"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/(SICI)1097-4636(19990605)45:3<268::AID-JBM15>3.0.CO;2-Q","article-title":"Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle","volume":"45","author":"WT Godbey","year":"1999","journal-title":"J Biomed Mater Res A"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1038\/emboj.2011.286","article-title":"Endosome maturation","volume":"30","author":"J Huotari","year":"2011","journal-title":"EMBO J"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"17707","DOI":"10.1016\/S0021-9258(19)47429-2","article-title":"Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells","volume":"266","author":"T Yoshimori","year":"1991","journal-title":"J Biol Chem"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1021\/nn900002m","article-title":"Impact of nanotechnology on drug delivery","volume":"3","author":"OC Farokhzad","year":"2009","journal-title":"ACS nano"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1002\/anie.200802585","article-title":"Nanomedicine\u2014challenge and perspectives","volume":"48","author":"K Riehemann","year":"2009","journal-title":"Angew Chem Int Ed"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jconrel.2013.03.010","article-title":"Immune system targeting by biodegradable nanoparticles for cancer vaccines","volume":"168","author":"JM Silva","year":"2013","journal-title":"J Control Release"},{"key":"ref72","first-page":"1","article-title":"Vaccine delivery using nanoparticles","volume":"25","author":"AE Gregory","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.1039\/C5TB00618J","article-title":"The potential of nanoparticles for the immunization against viral infections","volume":"3","author":"V Sokolova","year":"2015","journal-title":"J Mater Chem B"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1517\/17425247.2015.963055","article-title":"Non-spherical micro- and nanoparticles: fabrication, characterization and drug delivery applications","volume":"12","author":"R Mathaes","year":"2015","journal-title":"Exp Opin Drug Deliv"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1515\/bnm-2013-0020","article-title":"Biological targeting with nanoparticles: state of the art","volume":"14","author":"D Kozlova","year":"2013","journal-title":"BioNanoMaterials"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1021\/bp049648w","article-title":"Cellular fate of a modular DNA delivery system mediated by silica nanoparticles","volume":"21","author":"RA Gemeinhart","year":"2005","journal-title":"Biotechnol Prog"},{"key":"ref77","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1096\/fj.02-0088com","article-title":"Rapid endo-lysosomal escape of poly(DL-lactide-co-glycolide) nanoparticles: implications for drug and gene delivery","volume":"16","author":"J Panyam","year":"2002","journal-title":"FASEB J"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2174\/156720106776359159","article-title":"Delivery strategies for siRNA-mediated gene silencing","volume":"3","author":"IR Gilmore","year":"2006","journal-title":"Curr Drug Deliv"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.copbio.2010.07.003","article-title":"Chemical vectors for gene delivery: uptake and intracellular trafficking","volume":"21","author":"C Pichon","year":"2010","journal-title":"Curr Opin Biotechnol"},{"key":"ref80","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1016\/j.actbio.2008.09.009","article-title":"Characterization of a multifunctional PEG-based gene delivery system containing nuclear localization signals and endosomal escape peptides","volume":"5","author":"NM Moore","year":"2009","journal-title":"Acta Biomater"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1002\/jgm.696","article-title":"Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis","volume":"7","author":"A Akinc","year":"2005","journal-title":"J Gene Med"},{"key":"ref82","doi-asserted-by":"crossref","first-page":"18102","DOI":"10.1039\/C5RA25333K","article-title":"Nanoparticles as transfection agents: a comprehensive study with ten different cell lines","volume":"6","author":"B Neuhaus","year":"2016","journal-title":"RSC Adv"},{"key":"ref83","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.bbabio.2009.02.018","article-title":"Probing the pH sensitivity of R-phycoerythrin: Investigations of active conformational and functional variation","volume":"1787","author":"LN Liu","year":"2009","journal-title":"Biochim Biophys Acta\u2014Bioenergetics"},{"key":"ref84","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1146\/annurev-physiol-012110-142317","article-title":"Lysosomal acidification mechanisms","volume":"74","author":"JA Mindell","year":"2012","journal-title":"Ann Rev Physiol"},{"key":"ref85","article-title":"Live-cell imaging to compare the transfection and gene silencing efficiency of calcium phosphate nanoparticles and a liposomal transfection agent","author":"S Chernousova","year":"2017","journal-title":"Gene Ther"},{"key":"ref86","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.nantod.2011.02.003","article-title":"Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies","volume":"6","author":"TG Iversen","year":"2011","journal-title":"Nano Today"},{"key":"ref87","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.addr.2007.06.003","article-title":"Biodegradable nanoparticles for cytosolic delivery of therapeutics","volume":"59","author":"JK Vasir","year":"2007","journal-title":"Adv Drug Delivery Rev"},{"key":"ref88","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.3762\/bjnano.7.121","article-title":"On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles","volume":"7","author":"C Messerschmidt","year":"2016","journal-title":"Beilstein J Nanotechnol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0178260","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T16:08:04Z","timestamp":1719245284000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0178260"}},"subtitle":[],"editor":[{"given":"Jonghoon","family":"Choi","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,6,6]]},"references-count":88,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2017,6,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0178260","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,6]]}}}