{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:43:00Z","timestamp":1767339780214,"version":"3.37.3"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T00:00:00Z","timestamp":1499212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007652","name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","doi-asserted-by":"publisher","award":["RTA2014-00002-C02-01 (FEDER co-funded)"],"award-info":[{"award-number":["RTA2014-00002-C02-01 (FEDER co-funded)"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0180546","type":"journal-article","created":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T13:49:50Z","timestamp":1499262590000},"page":"e0180546","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep"],"prefix":"10.1371","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5121-7264","authenticated-orcid":true,"given":"Ana","family":"Balseiro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Altuzarra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enric","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Moll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Espada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iker A.","family":"Sevilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano","family":"Domingo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseba M.","family":"Garrido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n A.","family":"Juste","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Prieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernat","family":"P\u00e9rez de Val","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,7,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1365-2672.1990.tb02892.x","article-title":"A review. The avian tubercle bacillus and its relatives","volume":"68","author":"JM Grange","year":"1990","journal-title":"J Appl Bacteriol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1136\/vr.102581","article-title":"Vaccination against tuberculosis in badgers and cattle: an overview of the challenges, developments and current research priorities in Great Britain","volume":"175","author":"MA Chambers","year":"2014","journal-title":"Vet Rec"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"71","DOI":"10.20506\/rst.20.1.1263","article-title":"<italic>Mycobacterium bovis<\/italic> infection and control in domestic livestock","volume":"20","author":"DV Cousins","year":"2001","journal-title":"Rev Sci Technol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.vetmic.2011.02.041","article-title":"Progress in the control of bovine tuberculosis in wildlife in Spain","volume":"151","author":"C Gort\u00e1zar","year":"2011","journal-title":"Vet Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1111\/tbed.12325","article-title":"Sheep as a potential source of bovine TB: epidemiology, pathology and evaluation of diagnostic techniques","volume":"63","author":"M Mu\u00f1oz-Mendoza","year":"2016","journal-title":"Transbound Emerg Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0034-5288(02)00038-3","article-title":"The efficacy of bacille Calmette-Guerin vaccine in wild brushtail possums (<italic>Trichosurus vulpecula<\/italic>)","volume":"73","author":"LAL Corner","year":"2002","journal-title":"Res Vet Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"6589","DOI":"10.1016\/j.vaccine.2007.06.056","article-title":"Vaccination of white-tailed deer (<italic>Odocoileus virginianus<\/italic>) with <italic>Mycobacterium bovis<\/italic> bacillus Calmette Guer\u00edn","volume":"25","author":"MV Palmer","year":"2007","journal-title":"Vaccine"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"247","DOI":"10.7589\/0090-3558-44.2.247","article-title":"Efficacy of oral and parenteral routes of <italic>Mycobacterium bovis<\/italic> bacilli Calmette-Guerin vaccination against experimental bovine tuberculosis in white-tailed deer (<italic>Odocoileus virginianus<\/italic>): a feseability study","volume":"44","author":"P Nol","year":"2008","journal-title":"J Wild Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"6662","DOI":"10.1016\/j.vaccine.2009.08.095","article-title":"First data on Eurasian wild boar response to oral immunization with BCG and challenge with a <italic>Mycobacterium bovis<\/italic> field strain","volume":"27","author":"C Ballesteros","year":"2009","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2987","DOI":"10.1098\/rspb.2009.0414","article-title":"Oral vaccination reduces the incidence of tuberculosis in free-living brushtail possums","volume":"276","author":"DM Tompkins","year":"2009","journal-title":"P Roy Soc Lon B Bio"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.rvsc.2009.05.022","article-title":"Field evaluation of the protective efficacy of <italic>Mycobacterium bovis<\/italic> BCG vaccine against bovine tuberculosis","volume":"88","author":"G L\u00f3pez-Valencia","year":"2010","journal-title":"Res Vet Sci"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1098\/rspb.2010.1953","article-title":"Bacillus Calmette-Guerin vaccination reduces the severity and progression of tuberculosis in badgers","volume":"278","author":"M Chambers","year":"2011","journal-title":"P Roy Soc Lon B Bio"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1016\/j.vaccine.2012.02.018","article-title":"Bovine tuberculosis vaccine research: historical perspectives and recent advances","volume":"30","author":"WR Waters","year":"2012","journal-title":"Vaccine"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e81317","DOI":"10.1371\/journal.pone.0081317","article-title":"A multi-antigenic adenoviral-vectored vaccine improves BCG-induced protection of goats against pulmonary tuberculosis infection and prevents disease progression","volume":"8","author":"B P\u00e9rez de Val","year":"2013","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.tvjl.2014.02.031","article-title":"Oral vaccination of badgers (<italic>Meles meles<\/italic>) against tuberculosis: comparison of the protection generated by BCG vaccine strains Pasteur and Danish","volume":"200","author":"D Murphy","year":"2014","journal-title":"Vet J"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/j.vaccine.2016.01.004","article-title":"Assessment of safety and interferon gamma responses of <italic>Mycobacterium bovis<\/italic> BCG vaccine in goat kids and milking goats","volume":"34","author":"B P\u00e9rez de Val","year":"2016","journal-title":"Vaccine"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"S53","DOI":"10.1016\/j.rvsc.2014.04.015","article-title":"Vaccination of domestic animals against tuberculosis: review of progress and contributions to the field of the TBSTEP project","volume":"97","author":"HM Vordermeier","year":"2014","journal-title":"Res Vet Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e24905","DOI":"10.1371\/journal.pone.0024905","article-title":"Protection against tuberculosis in Eurasian wild boar vaccinated with heat-inactivated <italic>Mycobacterium bovis<\/italic>","volume":"6","author":"JM Garrido","year":"2011","journal-title":"PLoS One"},{"key":"ref19","unstructured":"Council Directive 64\/432\/EEC. On animal health problems affecting intra-community trade in bovine animals and swine. 1964. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.vet.gov.ba\/pdffiles\/eu_leg\/anheu17.pdf\" xlink:type=\"simple\">http:\/\/www.vet.gov.ba\/pdffiles\/eu_leg\/anheu17.pdf<\/ext-link>"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/tbed.12368","article-title":"Serological follow-up of tuberculosis in a wild boar population in contact with infected cattle","volume":"64","author":"B P\u00e9rez de Val","year":"2017","journal-title":"Trans Emerg Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1128\/CVI.05323-11","article-title":"Experimental model of tuberculosis in the domestic goat after endobronchial infection with <italic>Mycobacterium caprae<\/italic>","volume":"18","author":"B P\u00e9rez de Val","year":"2011","journal-title":"Clin Vac Immunol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1101\/gr.1.4.269","article-title":"Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube","volume":"1","author":"S Wilton","year":"1992","journal-title":"PCR Methods Appl"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1080\/00480169.1981.34798","article-title":"Observations on tuberculosis caused by Mycobacterium bovis in sheep","volume":"29","author":"DO Cordes","year":"1981","journal-title":"NZ Vet J"},{"key":"ref24","first-page":"523","article-title":"Pathology of Domestic Animals","author":"JL Caswell","year":"2007"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.rvsc.2006.10.016","article-title":"Experimental tuberculosis in the European badger (<italic>Meles meles<\/italic>) after endobronchial inoculation of <italic>Mycobacterium bovis<\/italic>: I. Pathology and bacteriology","volume":"83","author":"LA Corner","year":"2007","journal-title":"Res Vet Sci"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1128\/IAI.00287-09","article-title":"Viral booster vaccines improve <italic>Mycobacterium bovis<\/italic> BCG-induced protection against bovine tuberculosis","volume":"77","author":"HM Vordermeier","year":"2009","journal-title":"Infect Immun"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1146\/annurev-animal-021815-111311","article-title":"Bovine tuberculosis in cattle: vaccines, DIVA tests, and host biomarker discovery","volume":"4","author":"HM Vordermeier","year":"2016","journal-title":"Annu Rev Anim Biosc"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1054\/tuld.1998.0202","article-title":"Vaccine protocols to optimise the protective efficacy of BCG","volume":"79","author":"JF Griffin","year":"1999","journal-title":"Tuber Lung Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.vaccine.2003.05.002","article-title":"A single dose of killed <italic>Mycobacterium bovis<\/italic> BCG in a novel class of adjuvant (Novasome) protects guinea pigs from lethal tuberculosis","volume":"22","author":"MA Chambers","year":"2004","journal-title":"Vaccine"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetimm.2016.03.003","article-title":"Oral administration of heat-inactivated <italic>Mycobacterium bovis<\/italic> reduces the response of farmed deer to avian and bovine tuberculin","volume":"172","author":"V L\u00f3pez","year":"2016","journal-title":"Vet Immunol Immunopathol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.vetimm.2016.10.010","article-title":"Oral vaccination of cattle with heat inactivated <italic>Mycobacterium bovis<\/italic> does not compromise bovine TB diagnostic tests","volume":"182","author":"GJ Jones","year":"2016","journal-title":"Vet Immunol Immunopathol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1128\/IAI.72.5.2462-2467.2004","article-title":"Association of tuberculin-boosted antibody responses with pathology and cell-mediated immunity in cattle vaccinated with <italic>Mycobacterium bovis<\/italic> BCG and infected with <italic>M-bovis<\/italic>","volume":"72","author":"K Lyashchenko","year":"2004","journal-title":"Infect Immun"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1128\/CVI.00061-06","article-title":"Early Antibody Responses to Experimental <italic>Mycobacterium bovis<\/italic> Infection of Cattle","volume":"13","author":"WR Waters","year":"2006","journal-title":"Clin Vaccine Immunol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0180546","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T09:57:11Z","timestamp":1540029431000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0180546"}},"subtitle":[],"editor":[{"given":"Pere-Joan","family":"Cardona","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,7,5]]},"references-count":33,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0180546","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2017,7,5]]}}}