{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T04:18:15Z","timestamp":1759033095486},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T00:00:00Z","timestamp":1499644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0180960","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T17:48:10Z","timestamp":1499708890000},"page":"e0180960","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Increased transgene expression level of rabies virus vector for transsynaptic tracing"],"prefix":"10.1371","volume":"12","author":[{"given":"Shinya","family":"Ohara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Sota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken-Ichiro","family":"Tsutsui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Iijima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,7,10]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0165-0270(00)00295-8","article-title":"Use of pseudorabies virus to delineate multisynaptic circuits in brain: Opportunities and limitations","volume":"103","author":"G Aston-Jones","year":"2000","journal-title":"J Neurosci Methods"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/0166-2236(90)90071-H","article-title":"Viruses as transneuronal tracers","volume":"13","author":"HGJM Kuypers","year":"1990","journal-title":"Trends Neurosci"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0165-0270(00)00296-X","article-title":"Rabies as a transneuronal tracer of circuits in the central nervous system","volume":"103","author":"RM Kelly","year":"2000","journal-title":"J Neurosci Methods"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.conb.2009.03.007","article-title":"Transneuronal circuit tracing with neurotropic viruses","volume":"18","author":"EM Callaway","year":"2008","journal-title":"Curr Opin Neurobiol"},{"issue":"JAN","key":"ref5","first-page":"344","article-title":"Untangling neural networks with dual retrograde transsynaptic viral infection","volume":"4","author":"S Ohara","year":"2010","journal-title":"Front Neurosci"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jneumeth.2009.12.001","article-title":"Advances in viral transneuronal tracing","volume":"194","author":"G Ugolini","year":"2010","journal-title":"J Neurosci Methods"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0166-0934(02)00249-5","article-title":"An improved method for recovering rabies virus from cloned cDNA","volume":"107","author":"KI Inoue","year":"2003","journal-title":"J Virol Methods"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1128\/JVI.02327-07","article-title":"The glycoprotein and the matrix protein of rabies virus affect pathogenicity by regulating viral replication and facilitating cell-to-cell spread","volume":"82","author":"R Pulmanausahakul","year":"2008","journal-title":"J Virol"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1128\/JVI.73.1.510-518.1999","article-title":"Pathogenicity of different rabies virus variants inversely correlates with apoptosis and rabies virus glycoprotein expression in infected primary neuron cultures","volume":"73","author":"K Morimoto","year":"1999","journal-title":"J Virol"},{"key":"ref10","first-page":"12","article-title":"Development of rabies virus vectors for neuron-specific and transsynaptic delivery of a foreign gene","volume":"124","author":"K Inoue","year":"2004","journal-title":"Soc Neurosci Abstr"},{"issue":"11","key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0080245","article-title":"Rabies virus vector transgene expression level and cytotoxicity improvement induced by deletion of glycoprotein gene","volume":"8","author":"S Ohara","year":"2013","journal-title":"PLoS One"},{"issue":"9","key":"ref12","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1099\/0022-1317-81-9-2147","article-title":"Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: An in vitro and in vivo study","volume":"81","author":"R Etessami","year":"2000","journal-title":"J Gen Virol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/nmeth999","article-title":"Retrograde neuronal tracing with a deletion-mutant rabies virus","volume":"4","author":"IR Wickersham","year":"2007","journal-title":"Nat Methods"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.neuron.2016.01.004","article-title":"Rabies Virus CVS-N2c\u03b4G Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability","volume":"89","author":"TR Reardon","year":"2016","journal-title":"Neuron [Internet]"},{"issue":"January","key":"ref15","first-page":"47","article-title":"Rabies virus glycoprotein variants display different patterns in rabies monosynaptic tracing","volume":"7","author":"T Mori","year":"2014","journal-title":"Front Neuroanat"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/neuro.05.001.2009","article-title":"Dual transneuronal tracing in the rat entorhinal-hippocampal circuit by intracerebral injection of recombinant rabies virus vectors","volume":"3","author":"S Ohara","year":"2009","journal-title":"Front Neuroanat"},{"issue":"11","key":"ref17","doi-asserted-by":"crossref","first-page":"e78928","DOI":"10.1371\/journal.pone.0078928","article-title":"Organization of multisynaptic inputs to the dorsal and ventral dentate gyrus: retrograde trans-synaptic tracing with rabies virus vector in the rat","volume":"8","author":"S Ohara","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/nchembio.138","article-title":"Monomeric fluorescent timers that change color from blue to red report on cellular trafficking","volume":"5","author":"F V Subach","year":"2009","journal-title":"Nat Chem Biol"},{"issue":"16","key":"ref19","doi-asserted-by":"crossref","first-page":"7261","DOI":"10.1128\/JVI.74.16.7261-7269.2000","article-title":"Differential transcription attenuation of rabies virus genes by intergenic regions: generation of recombinant viruses overexpressing the polymerase gene","volume":"74","author":"S Finke","year":"2000","journal-title":"J Virol"},{"issue":"2 SPEC. ISS.","key":"ref20","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.virusres.2005.04.004","article-title":"Replication strategies of rabies virus","volume":"111","author":"S Finke","year":"2005","journal-title":"Virus Res"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0128020","article-title":"Effects of G-gene deletion and replacement on rabies virus vector gene expression","volume":"10","author":"S Sato","year":"2015","journal-title":"PLoS One"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1128\/JVI.73.1.242-250.1999","article-title":"Matrix protein of rabies virus is responsible for the assembly and budding of bullet-shaped particles and interacts with the transmembrane spike glycoprotein G","volume":"73","author":"T Mebatsion","year":"1999","journal-title":"J Virol"},{"issue":"22","key":"ref23","doi-asserted-by":"crossref","first-page":"12074","DOI":"10.1128\/JVI.77.22.12074-12082.2003","article-title":"Dissociation of rabies virus matrix protein functions in regulation of viral RNA synthesis and virus assembly","volume":"77","author":"S Finke","year":"2003","journal-title":"J Virol"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1099\/vir.0.19128-0","article-title":"Rabies virus matrix protein regulates the balance of virus transcription and replication","volume":"84","author":"S Finke","year":"2003","journal-title":"J Gen Virol"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1073\/pnas.73.5.1504","article-title":"Sequential transcription of the genes of vesicular stomatitis virus","volume":"73","author":"G Abraham","year":"1976","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1073\/pnas.73.2.442","article-title":"Order of transcription of genes of vesicular stomatitis virus","volume":"73","author":"LA Ball","year":"1976","journal-title":"Pnas"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/0092-8674(81)90143-4","article-title":"Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription","volume":"23","author":"LE Iverson","year":"1981","journal-title":"Cell"},{"issue":"12","key":"ref28","doi-asserted-by":"crossref","first-page":"7877","DOI":"10.1073\/pnas.082243699","article-title":"A monomeric red fluorescent protein","volume":"99","author":"RE Campbell","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref29","first-page":"199","article-title":"Antigenic analysis of rabies and Mokola virus from Zimbabwe using monoclonal antibodies","volume":"57","author":"TJ Wiktor","year":"1984","journal-title":"Dev Biol Stand"},{"issue":"8","key":"ref30","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1046\/j.1440-1827.2003.01511.x","article-title":"Cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific antirabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues","volume":"53","author":"S Inoue","year":"2003","journal-title":"Pathol Int"},{"issue":"11","key":"ref31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0078928","article-title":"Organization of multisynaptic inputs to the dorsal and ventral dentate gyrus: Retrograde trans-synaptic tracing with rabies virus vector in the rat","volume":"8","author":"S Ohara","year":"2013","journal-title":"PLoS One"},{"issue":"7","key":"ref32","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1128\/JVI.76.7.3374-3381.2002","article-title":"Overexpression of the Rabies Virus Glycoprotein Results in Enhancement of Apoptosis and Antiviral Immune Response","volume":"76","author":"M Faber","year":"2002","journal-title":"J Virol"},{"issue":"19","key":"ref33","doi-asserted-by":"crossref","first-page":"10537","DOI":"10.1128\/JVI.77.19.10537-10547.2003","article-title":"Glycoprotein of nonpathogenic rabies viruses is a key determinant of human cell apoptosis","volume":"77","author":"C Pr\u00e9haud","year":"2003","journal-title":"J Virol"},{"issue":"5","key":"ref34","doi-asserted-by":"crossref","first-page":"481","DOI":"10.2217\/17460794.3.5.481","article-title":"Concepts in the pathogenesis of rabies","volume":"3","author":"B Dietzschold","year":"2008","journal-title":"Future Virol"},{"issue":"7563","key":"ref35","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/nature14600","article-title":"Viral-genetic tracing of the input\u2014output organization of a central noradrenaline circuit","volume":"524","author":"LA Schwarz","year":"2015","journal-title":"Nature"},{"issue":"24","key":"ref36","doi-asserted-by":"crossref","first-page":"8979","DOI":"10.1523\/JNEUROSCI.0409-15.2015","article-title":"Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses","volume":"35","author":"EM Callaway","year":"2015","journal-title":"J Neurosci"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0180960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,11]],"date-time":"2020-10-11T23:02:17Z","timestamp":1602457337000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0180960"}},"subtitle":[],"editor":[{"given":"Eric J","family":"Kremer","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,7,10]]},"references-count":36,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0180960","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,10]]}}}