{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T17:10:31Z","timestamp":1778260231885,"version":"3.51.4"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T00:00:00Z","timestamp":1499731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0180990","type":"journal-article","created":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T17:38:18Z","timestamp":1499794698000},"page":"e0180990","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species"],"prefix":"10.1371","volume":"12","author":[{"given":"Yohsuke","family":"Nagayoshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0962-5758","authenticated-orcid":true,"given":"Taiga","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shintaro","family":"Shimamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asuka","family":"Minematsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunsuke","family":"Yamauchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Takazono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsunori","family":"Yanagihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Kohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Mukae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Izumikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,7,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1128\/CMR.00029-06","article-title":"Epidemiology of invasive candidiasis: a persistent public health problem","volume":"20","author":"MA Pfaller","year":"2007","journal-title":"Clin Microbiol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1128\/AAC.48.5.1773-1777.2004","article-title":"Mechanism of increased fluconazole resistance in <italic>Candida glabrata<\/italic> during prophylaxis","volume":"48","author":"JE Bennett","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1128\/AAC.43.11.2753","article-title":"The ATP binding cassette transporter gene <italic>CgCDR1<\/italic> from <italic>Candida glabrata<\/italic> is involved in the resistance of clinical isolates to azole antifungal agents","volume":"43","author":"D Sanglard","year":"1999","journal-title":"Antimicrob Agents Chemother"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1128\/AAC.42.7.1695","article-title":"Fluconazole resistance associated with drug efflux and increased transcription of a drug transporter gene, <italic>PDH1<\/italic>, in <italic>Candida glabrata<\/italic>","volume":"42","author":"H Miyazaki","year":"1998","journal-title":"Antimicrob Agents Chemother"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1128\/AAC.05706-11","article-title":"The monoamine oxidase A inhibitor clorgyline is a broad-spectrum inhibitor of fungal ABC and MFS transporter efflux pump activities which reverses the azole resistance of <italic>Candida albicans<\/italic> and <italic>Candida glabrata<\/italic> clinical isolates","volume":"56","author":"AR Holmes","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1016\/j.addr.2008.06.002","article-title":"Monoamine oxidase inactivation: from pathophysiology to therapeutics","volume":"60","author":"M Bortolato","year":"2008","journal-title":"Adv Drug Deliv Rev"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1161\/CIRCRESAHA.109.198366","article-title":"Monoamine oxidase A-mediated enhanced catabolism of norepinephrine contributes to adverse remodeling and pump failure in hearts with pressure overload","volume":"106","author":"N Kaludercic","year":"2010","journal-title":"Circ Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1007\/s00432-010-0835-6","article-title":"Targeting monoamine oxidase A in advanced prostate cancer","volume":"136","author":"V Flamand","year":"2010","journal-title":"J Cancer Res Clin Oncol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1086\/596757","article-title":"Clinical practice guidelines for the management of candidiasis: 2009 update by the Infectious Diseases Society of America","volume":"48","author":"PG Pappas","year":"2009","journal-title":"Clin Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1128\/JCM.06112-11","article-title":"Frequency of decreased susceptibility and resistance to echinocandins among fluconazole-resistant bloodstream isolates of <italic>Candida glabrata<\/italic>","volume":"50","author":"MA Pfaller","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1590\/S0074-02762012000300022","article-title":"The activity of echinocandins, amphotericin B and voriconazole against fluconazole-susceptible and fluconazole-resistant Brazilian <italic>Candida glabrata<\/italic> isolates","volume":"107","author":"DA Mario","year":"2012","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1093\/jac\/dkl237","article-title":"Caspofungin activity against clinical isolates of azole cross-resistant <italic>Candida glabrata<\/italic> overexpressing efflux pump genes","volume":"58","author":"B Posteraro","year":"2006","journal-title":"J Antimicrob Chemother"},{"key":"ref13","article-title":"Laboratory Course Manual for Methods in Yeast Genetics","author":"C Kaiser","year":"1994"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1567-1364.2010.00611.x","article-title":"Role of the Slt2 mitogen-activated protein kinase pathway in cell wall integrity and virulence in <italic>Candida glabrata<\/italic>","volume":"10","author":"T Miyazaki","year":"2010","journal-title":"FEMS Yeast Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1111\/j.1567-1364.2011.00751.x","article-title":"Functional characterization of the regulators of calcineurin in <italic>Candida glabrata<\/italic>","volume":"11","author":"T Miyazaki","year":"2011","journal-title":"FEMS Yeast Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1128\/AAC.01364-09","article-title":"Roles of calcineurin and Crz1 in antifungal susceptibility and virulence of <italic>Candida glabrata<\/italic>","volume":"54","author":"T Miyazaki","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1093\/genetics\/151.3.979","article-title":"Efficient homologous and illegitimate recombination in the opportunistic yeast pathogen <italic>Candida glabrata<\/italic>","volume":"151","author":"BP Cormack","year":"1999","journal-title":"Genetics"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1128\/AAC.48.3.693-715.2004","article-title":"Combination antifungal therapy","volume":"48","author":"MD Johnson","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e1003160","DOI":"10.1371\/journal.ppat.1003160","article-title":"Dissection of Ire1 functions reveals stress response mechanisms uniquely evolved in <italic>Candida glabrata<\/italic>","volume":"9","author":"T Miyazaki","year":"2013","journal-title":"PLoS Pathog"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1093\/jac\/dkn156","article-title":"Micafungin activity against <italic>Candida albicans<\/italic> with diverse azole resistance phenotypes","volume":"62","author":"TS Richards","year":"2008","journal-title":"J Antimicrob Chemother"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1046\/j.1365-2958.2003.03430.x","article-title":"The <italic>Candida albicans<\/italic> Cdr2p ATP-binding cassette (ABC) transporter confers resistance to caspofungin","volume":"48","author":"M Schuetzer-Muehlbauer","year":"2003","journal-title":"Mol Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1128\/AAC.50.4.1148-1155.2006","article-title":"Overexpression of <italic>Candida albicans CDR1<\/italic>, <italic>CDR2<\/italic>, or <italic>MDR1<\/italic> does not produce significant changes in echinocandin susceptibility","volume":"50","author":"K Niimi","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1128\/JCM.00308-13","article-title":"Echinocandin and triazole antifungal susceptibility profiles for clinical opportunistic yeast and mold isolates collected from 2010 to 2011: application of new CLSI clinical breakpoints and epidemiological cutoff values for characterization of geographic and temporal trends of antifungal resistance","volume":"51","author":"MA Pfaller","year":"2013","journal-title":"J Clin Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1128\/JCM.40.6.2228-2230.2002","article-title":"In vitro activity of caspofungin (MK-0991) against <italic>Candida albicans<\/italic> clinical isolates displaying different mechanisms of azole resistance","volume":"40","author":"SP Bachmann","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/CMR.00051-08","article-title":"Efflux-mediated antifungal drug resistance","volume":"22","author":"RD Cannon","year":"2009","journal-title":"Clin Microbiol Rev"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1128\/AAC.50.4.1384-1392.2006","article-title":"<italic>Candida glabrata PDR1<\/italic>, a transcriptional regulator of a pleiotropic drug resistance network, mediates azole resistance in clinical isolates and petite mutants","volume":"50","author":"HF Tsai","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1128\/AAC.45.4.1174-1183.2001","article-title":"Role of ATP-binding-cassette transporter genes in high-frequency acquisition of resistance to azole antifungals in <italic>Candida glabrata<\/italic>","volume":"45","author":"D Sanglard","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1128\/AAC.00861-07","article-title":"Multidrug transporters CaCdr1p and CaMdr1p of <italic>Candida albicans<\/italic> display different lipid specificities: both ergosterol and sphingolipids are essential for targeting of CaCdr1p to membrane rafts","volume":"52","author":"R Pasrija","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.1128\/AAC.48.5.1778-1787.2004","article-title":"Membrane sphingolipid-ergosterol interactions are important determinants of multidrug resistance in <italic>Candida albicans<\/italic>","volume":"48","author":"K Mukhopadhyay","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2958.2012.08194.x","article-title":"CRS-MIS in <italic>Candida glabrata<\/italic>: sphingolipids modulate echinocandin-Fks interaction","volume":"86","author":"KR Healey","year":"2012","journal-title":"Mol Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1128\/AAC.04667-14","article-title":"Sphingolipids mediate differential echinocandin susceptibility in <italic>Candida albicans<\/italic> and <italic>Aspergillus nidulans<\/italic>","volume":"59","author":"KR Healey","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1073\/pnas.90.5.1902","article-title":"Cloning and expression of the gene from <italic>Candida albicans<\/italic> that encodes a high-affinity corticosteroid-binding protein","volume":"90","author":"PJ Malloy","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s002940050109","article-title":"Characterization of the <italic>Saccharomyces cerevisiae FMS1<\/italic> gene related to <italic>Candida albicans<\/italic> corticosteroid-binding protein 1","volume":"30","author":"J Joets","year":"1996","journal-title":"Curr Genet"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1128\/AAC.02040-12","article-title":"Milbemycins: more than efflux inhibitors for fungal pathogens","volume":"57","author":"LV Silva","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1128\/EC.00355-09","article-title":"Fluconazole transport into <italic>Candida albicans<\/italic> secretory vesicles by the membrane proteins Cdr1p, Cdr2p, and Mdr1p","volume":"9","author":"LR Basso Jr.","year":"2010","journal-title":"Eukaryot Cell"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/j.1567-1364.2009.00508.x","article-title":"Dynamics of in vitro acquisition of resistance by <italic>Candida parapsilosis<\/italic> to different azoles","volume":"9","author":"AT Pinto e Silva","year":"2009","journal-title":"FEMS Yeast Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1080\/13693780600561544","article-title":"Fluconazole resistance mechanisms in <italic>Candida krusei<\/italic>: the contribution of efflux-pumps","volume":"44","author":"J Guinea","year":"2006","journal-title":"Med Mycol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/(SICI)1097-0320(20000101)39:1<26::AID-CYTO5>3.0.CO;2-C","article-title":"Rapid detection of efflux pumps and their relation with drug resistance in yeast cells","volume":"39","author":"C Prudencio","year":"2000","journal-title":"Cytometry"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nature02579","article-title":"Genome evolution in yeasts","volume":"430","author":"B Dujon","year":"2004","journal-title":"Nature"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0378-1119(95)00552-H","article-title":"Cloning of the <italic>Candida glabrata TRP1<\/italic> and <italic>HIS3<\/italic> genes, and construction of their disruptant strains by sequential integrative transformation","volume":"165","author":"K Kitada","year":"1995","journal-title":"Gene"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1128\/EC.00414-06","article-title":"Development of a highly efficient gene targeting system induced by transient repression of <italic>YKU80<\/italic> expression in <italic>Candida glabrata<\/italic>","volume":"6","author":"K Ueno","year":"2007","journal-title":"Eukaryot Cell"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"7329","DOI":"10.1073\/pnas.0401648101","article-title":"The diploid genome sequence of <italic>Candida albicans<\/italic>","volume":"101","author":"T Jones","year":"2004","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0180990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T19:37:59Z","timestamp":1659209879000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0180990"}},"subtitle":[],"editor":[{"given":"Ilse D.","family":"Jacobsen","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,7,11]]},"references-count":42,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0180990","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,11]]}}}