{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T08:42:57Z","timestamp":1789548177464,"version":"build-2803163510"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d\u2019Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["DI2013-0039"],"award-info":[{"award-number":["DI2013-0039"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0181194","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T17:43:50Z","timestamp":1501004630000},"page":"e0181194","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Determinants for swine mycoplasmal pneumonia reproduction under experimental conditions: A systematic review and recursive partitioning analysis"],"prefix":"10.1371","volume":"12","author":[{"given":"Beatriz","family":"Garcia-Morante","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim","family":"Segal\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Serrano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3867-1988","authenticated-orcid":true,"given":"Marina","family":"Sibila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,7,25]]},"reference":[{"key":"ref1","first-page":"779","article-title":"Diseases of swine","author":"EL Thacker","year":"2012"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.vetmic.2007.09.008","article-title":"Control of <italic>Mycoplasma hyopneumoniae<\/italic> infections in pigs","volume":"126","author":"D Maes","year":"2008","journal-title":"Veterinary Microbiology"},{"key":"ref3","first-page":"371","article-title":"Molecular and Diagnostic Procedures in Mycoplasmology","author":"M Kobisch","year":"1996"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1354\/vp.41-6-624","article-title":"Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with <italic>Mycoplasma hyopneumoniae<\/italic> and porcine circovirus type 2","volume":"41","author":"T Opriessnig","year":"2004","journal-title":"Veterinary Pathology"},{"issue":"7","key":"ref5","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1128\/JCM.39.7.2525-2530.2001","article-title":"Interaction between <italic>Mycoplasma hyopneumoniae<\/italic> and swine influenza virus","volume":"39","author":"EL Thacker","year":"2001","journal-title":"Journal of Clinical Microbiology"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1128\/JCM.37.3.620-627.1999","article-title":"<italic>Mycoplasma hyopneumoniae<\/italic> potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia","volume":"37","author":"EL Thacker","year":"1999","journal-title":"Journal of Clinical Microbiology"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0378-1135(96)01266-7","article-title":"<italic>Mycoplasma hyopneumoniae<\/italic> infection in pigs: duration of the disease and evaluation of four diagnostic assays","volume":"54","author":"V Sorensen","year":"1997","journal-title":"Veterinary Microbiology"},{"issue":"1","key":"ref8","first-page":"67","article-title":"<italic>Mycoplasma hyopneumoniae<\/italic> infection in pigs: duration of the disease and resistance to reinfection","volume":"24","author":"M Kobisch","year":"1993","journal-title":"Veterinary Research"},{"issue":"1\u20132","key":"ref9","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetmic.2006.10.010","article-title":"Interactions of highly and low virulent <italic>Mycoplasma hyopneumoniae<\/italic> isolates with the respiratory tract of pigs","volume":"120","author":"T Meyns","year":"2007","journal-title":"Veterinary Microbiology"},{"issue":"3\u20134","key":"ref10","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetmic.2003.08.008","article-title":"Evaluation of virulence of <italic>Mycoplasma hyopneumoniae<\/italic> field isolates","volume":"97","author":"J Vicca","year":"2003","journal-title":"Veterinary Microbiology"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1136\/vr.150.1.9","article-title":"Induction of enzootic pneumonia in pigs by the administration of an aerosol of in vitro-cultured <italic>Mycoplasma hyopneumoniae<\/italic>","volume":"150","author":"T Czaja","year":"2002","journal-title":"The Veterinary Record"},{"issue":"5","key":"ref12","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1111\/j.1365-2672.2009.04556.x","article-title":"Development of a quantitative Real-Time TaqMan PCR assay for determination of the minimal dose of <italic>Mycoplasma hyopneumoniae<\/italic> strain 116 required to induce pneumonia in SPF pigs","volume":"108","author":"C Marois","year":"2010","journal-title":"Journal of applied microbiology"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13567-016-0340-2","article-title":"Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes","volume":"47","author":"B Garcia-Morante","year":"2016","journal-title":"Veterinary Research"},{"issue":"2\u20133","key":"ref14","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jcpa.2015.11.003","article-title":"Assessment of <italic>Mycoplasma hyopneumoniae<\/italic>-induced Pneumonia using Different Lung Lesion Scoring Systems: a Comparative Review","volume":"154","author":"B Garcia-Morante","year":"2016","journal-title":"Journal of Comparative Pathology"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Therneau T, Atkinson B, Ripley B. rpart: Recursive Partitioning and Regression Trees. R package version 4.1\u201310. 2015. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/cran.r-project.org\/web\/packages\/rpart\/rpart.pdf\" xlink:type=\"simple\">https:\/\/cran.r-project.org\/web\/packages\/rpart\/rpart.pdf<\/ext-link>.","DOI":"10.32614\/CRAN.package.rpart"},{"key":"ref16","unstructured":"Team RC. R: A language and environment for statistical computing. 2016. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.R-project.org\/\" xlink:type=\"simple\">https:\/\/www.R-project.org\/<\/ext-link>."},{"key":"ref17","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4419-6824-1","article-title":"Recursive Partitioning and Applications","author":"H Zhang","year":"2010"},{"issue":"11","key":"ref18","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1890\/0012-9658(2000)081[3178:CARTAP]2.0.CO;2","article-title":"Classification and regression trees: a powerful yet simple technique for ecological data analysis","volume":"81","author":"G De'ath","year":"2000","journal-title":"Ecology"},{"key":"ref19","doi-asserted-by":"crossref","DOI":"10.1201\/9781420010404","article-title":"Generalized Additive Models: An Introduction with R","author":"S Wood","year":"2006"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1467-9868.2010.00749.x","article-title":"Fast stable restricted maximum likelihood and marginal likelihood estimation of semiparametric generalized linear models","volume":"73","author":"S Wood","year":"2011","journal-title":"Journal of the Royal Statistical Society. Series B, Statistical Methodology"},{"key":"ref21","first-page":"011","article-title":"The establishment of an experimental swine model of swine mycoplasma pneumonia","volume":"4","author":"S Guo-qing","year":"2007","journal-title":"Journal of Microbes and Infection"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"b2535","DOI":"10.1136\/bmj.b2535","article-title":"Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement","volume":"339","author":"D Moher","year":"2009","journal-title":"BMJ (Clinical research ed)"},{"issue":"1\u20132","key":"ref23","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.vetmic.2012.07.025","article-title":"Evaluation of clinical, histological and immunological changes and qPCR detection of <italic>Mycoplasma hyopneumoniae<\/italic> in tissues during the early stages of mycoplasmal pneumonia in pigs after experimental challenge with two field isolates","volume":"161","author":"LK Woolley","year":"2012","journal-title":"Veterinary Microbiology"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1128\/CVI.00078-13","article-title":"Vaccination of piglets up to 1 week of age with a single-dose <italic>Mycoplasma hyopneumoniae<\/italic> vaccine induces protective immunity within 2 weeks against virulent challenge in the presence of maternally derived antibodies","volume":"20","author":"S Wilson","year":"2013","journal-title":"Clinical and Vaccine Immunology"},{"issue":"2\u20133","key":"ref25","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.jcpa.2012.01.003","article-title":"Simultaneous porcine circovirus type 2 and <italic>Mycoplasma hyopneumoniae<\/italic> co-inoculation does not potentiate disease in conventional pigs","volume":"147","author":"M Sibila","year":"2012","journal-title":"Journal of Comparative Pathology"},{"issue":"3\u20134","key":"ref26","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.vetmic.2008.05.023","article-title":"A meta-analysis on experimental infections with porcine circovirus type 2 (PCV2)","volume":"132","author":"A Tom\u00e1s","year":"2008","journal-title":"Veterinary Microbiology"},{"issue":"12","key":"ref27","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/j.vaccine.2008.12.005","article-title":"Infection with a low virulent <italic>Mycoplasma hyopneumoniae<\/italic> isolate does not protect piglets against subsequent infection with a highly virulent <italic>M<\/italic>. <italic>hyopneumoniae<\/italic> isolate","volume":"27","author":"I Villarreal","year":"2009","journal-title":"Vaccine"},{"issue":"2","key":"ref28","first-page":"157","article-title":"An experimental model to evaluate <italic>Mycoplasma hyopneumoniae<\/italic> transmission from asymptomatic carriers to unvaccinated and vaccinated sentinel pigs","volume":"74","author":"M Pieters","year":"2010","journal-title":"Canadian Journal of Veterinary Research"},{"issue":"3\u20134","key":"ref29","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.vetmic.2008.08.016","article-title":"An assessment of the duration of <italic>Mycoplasma hyopneumoniae<\/italic> infection in an experimentally infected population of pigs","volume":"134","author":"M Pieters","year":"2009","journal-title":"Veterinary Microbiology"},{"issue":"3","key":"ref30","first-page":"223","article-title":"Dynamics and persistence of <italic>Mycoplasma hyopneumoniae<\/italic> infection in pigs","volume":"69","author":"E Fano","year":"2005","journal-title":"Canadian Journal of Veterinary Research"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1099\/00207713-42-2-275","article-title":"Chromosomal heterogeneity of various <italic>Mycoplasma hyopneumoniae<\/italic> field strains","volume":"42","author":"J Frey","year":"1992","journal-title":"International Journal of Systematic Bacteriology"},{"issue":"11","key":"ref32","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.2460\/ajvr.1983.44.11.2087","article-title":"Comparison of <italic>Mycoplasma hyopneumoniae<\/italic> strains by serologic methods","volume":"44","author":"LH Ro","year":"1983","journal-title":"American Journal of Veterinary Research"},{"issue":"2\u20134","key":"ref33","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.vetmic.2013.11.006","article-title":"Genetic diversity of <italic>Mycoplasma hyopneumoniae<\/italic> isolates of abattoir pigs","volume":"168","author":"A Charlebois","year":"2014","journal-title":"Veterinary Microbiology"},{"issue":"5","key":"ref34","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1128\/JCM.00125-11","article-title":"Multiple-locus variable-number tandem-repeat analysis is a suitable tool for differentiation of <italic>Mycoplasma hyopneumoniae<\/italic> strains without cultivation","volume":"49","author":"K Vranckx","year":"2011","journal-title":"Journal of Clinical Microbiology"},{"issue":"2\u20133","key":"ref35","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0378-1135(90)90075-7","article-title":"Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against <italic>Mycoplasma hyopneumoniae<\/italic> in swine serum","volume":"25","author":"M Bereiter","year":"1990","journal-title":"Veterinary Microbiology"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"344","DOI":"10.2460\/ajvr.1990.51.03.344","article-title":"Effect of growth in cell cultures and strain on virulence of <italic>Mycoplasma hyopneumoniae<\/italic> for swine","volume":"51","author":"GC Zielinski","year":"1990","journal-title":"American Journal of Veterinary Research"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1746-6148-8-2","article-title":"Effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous <italic>Mycoplasma hyopneumoniae<\/italic> strains","volume":"8","author":"I Villarreal","year":"2012","journal-title":"BMC veterinary research"},{"issue":"10","key":"ref38","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.2460\/ajvr.1984.45.10.1899","article-title":"Characteristics of protective activity of <italic>Mycoplasma hyopneumoniae<\/italic> vaccine","volume":"45","author":"RF Ross","year":"1984","journal-title":"American Journal of Veterinary Research"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13567-016-0408-z","article-title":"Impact of diversity of <italic>Mycoplasma hyopneumoniae<\/italic> strains on lung lesions in slaughter pigs","volume":"48","author":"A Michiels","year":"2017","journal-title":"Veterinary Research"},{"issue":"4","key":"ref40","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1080\/00480169.2011.579553","article-title":"Inflammatory responses to <italic>Mycoplasma hyopneumoniae<\/italic> in murine alveolar macrophage cell lines","volume":"59","author":"D Damte","year":"2011","journal-title":"New Zealand Veterinary Journal"},{"issue":"3","key":"ref41","doi-asserted-by":"crossref","first-page":"478","DOI":"10.2460\/ajvr.1984.45.03.478","article-title":"Effect of age on susceptibility of pigs to <italic>Mycoplasma hyopneumoniae<\/italic> pneumonia","volume":"45","author":"IA Piffer","year":"1984","journal-title":"American Journal of Veterinary Research"},{"issue":"1\u20132","key":"ref42","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.vetimm.2010.11.019","article-title":"Cytokine and chemokine mRNA expression profiles in tracheobronchial lymph nodes from pigs singularly infected or coinfected with porcine circovirus type 2 (PCV2) and <italic>Mycoplasma hyopneumoniae<\/italic> (MHYO)","volume":"140","author":"H Zhang","year":"2011","journal-title":"Veterinary Immunology and Immunopathology"},{"issue":"5","key":"ref43","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1128\/CDLI.11.5.901-908.2004","article-title":"Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and <italic>Mycoplasma hyopneumoniae<\/italic>","volume":"11","author":"R Thanawongnuwech","year":"2004","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"issue":"3\u20134","key":"ref44","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.vetmic.2003.11.005","article-title":"Experimental dual infection of pigs with an H1N1 swine influenza virus (A\/Sw\/Hok\/2\/81) and <italic>Mycoplasma hyopneumoniae<\/italic>","volume":"98","author":"S Yazawa","year":"2004","journal-title":"Veterinary Microbiology"},{"key":"ref45","unstructured":"Fort M, Mombarg M, Balasch M, Taylor L, Urniza A, editors. Comparison of two different challenge procedures (transtracheal injection Vs. endotracheal inoculation) and two different inoculum preparations (lung homogenate Vs. fresh M. hyo. culture) for the induction of Mycoplasma hyopneumoniae infection in young pigs. Proceedings of the 5th European Symposium of Porcine Health Management; 2013 May 22\u201324; Edinburgh, United Kingdom."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0181194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T17:53:29Z","timestamp":1750787609000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0181194"}},"subtitle":[],"editor":[{"given":"Glenn F.","family":"Browning","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,7,25]]},"references-count":45,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0181194","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,25]]}}}