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Dis"},{"key":"ref8","first-page":"19","article-title":"Situaci\u00f3n del b\u00fafalo de agua en Costa Rica","volume":"24","author":"R Rosales-Rodr\u00edguez","year":"2011","journal-title":"Tecnolog\u00eda en Marcha (Costa Rica)"},{"key":"ref9","unstructured":"Rosales-Rodr\u00edguez R. Costa Rica. In: Crudeli GA, Konrad JL, Pati\u00f1o EM, editors. Reproducci\u00f3n en b\u00fafalas. 1st ed. Moglia.2016;251."},{"key":"ref10","unstructured":"Instituto Nacional de Estad\u00edsticas- INEC. VI Censo Nacional Agropecuario. San Jos\u00e9, Costa Rica. 2014;72\u201374."},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"May Collado L. Marine Mammals. In: Wehrtmann IS, Cortes J, editors. Marine biodiversity of Costa Rica, Central America. Monographiae Biologicae. 1st ed. Springer. 2009;479\u2013496.","DOI":"10.1007\/978-1-4020-8278-8_45"},{"key":"ref12","unstructured":"Alton GG, Jones LM, Angus RD, Verger JM. Techniques for brucellosis laboratory. INRA: Paris;1988."},{"key":"ref13","unstructured":"OIE-World Organization for Animal Health. Bovine brucellosis: In manual of diagnostic tests and vaccines for terrestrial animals. 12 rue de Prony 75017. Paris; 2009."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/CDLI.6.2.269-272.1999","article-title":"Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis","volume":"6","author":"CM Mar\u00edn","year":"1999","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref15","unstructured":"OIE-World Organization for Animal Health. Caprine and ovine brucellosis: In Manual of Diagnostic Tests and Vaccines. 12 rue de Prony 75017. Paris; 2009."},{"key":"ref16","unstructured":"Moreno E, Rojas N, Nielsen K, Gall D. Comparison of different serological assays for the differential diagnosis of brucellosis. In International Atomic Energy, Diagnosis and Epidemiology of Animal Diseases in Latin America IAEA-TECDOC 1055. Viena; 1998;153\u2013161."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1128\/CVI.00413-08","article-title":"Serological diagnosis of <italic>Brucella<\/italic> infections in odontocetes","volume":"16","author":"G Hern\u00e1ndez- Mora","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.vetimm.2012.02.012","article-title":"Assessment of performance of selected serological tests for diagnosing brucellosis in pigs","volume":"146","author":"PM Mu\u00f1oz","year":"2012","journal-title":"Vet Immunol Immunopathol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"385","DOI":"10.15517\/rbt.v55i2.6018","article-title":"Prevalencia de anticuerpos s\u00e9ricos contra la bacteria <italic>Brucella<\/italic> sp. en una poblaci\u00f3n humana tropical","volume":"55","author":"G Chanto","year":"2007","journal-title":"Rev Biol Trop"},{"key":"ref20","unstructured":"Reglamento para la intervenci\u00f3n de la brucelosis bovina. Decreto 34858\u2010MAG-2008. Gaceta, 230, (7-11- 2008) 2008; Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.pgrweb.go.cr\/scij\/Busqueda\/Normativa\/Normas\/nrm_texto_completo.aspx?param1=NRTC&nValor1=1&nValor2=64425&nValor3=74734&strTipM=TC\" xlink:type=\"simple\">http:\/\/www.pgrweb.go.cr\/scij\/Busqueda\/Normativa\/Normas\/nrm_texto_completo.aspx?param1=NRTC&amp;nValor1=1&amp;nValor2=64425&amp;nValor3=74734&amp;strTipM=TC<\/ext-link>"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1128\/JCM.02301-10","article-title":"Development of a selective culture medium for primary isolation of the main <italic>Brucella<\/italic> species","volume":"49","author":"MJ De Miguel","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1471-2180-6-9","article-title":"Evaluation and selection of tandem repeat loci for a <italic>Brucella<\/italic> MLVA typing assay","volume":"6","author":"P Le Fl\u00e8che","year":"2006","journal-title":"BMC Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1373\/clinchem.2005.062596","article-title":"Multiplex PCR Assay for the identification and differentiation of all <italic>Brucella<\/italic> Species and the vaccine strains <italic>Brucella abortus<\/italic> S19 and RB51 and <italic>Brucella melitensis<\/italic> Rev1","volume":"52","author":"D Garc\u00eda-Yoldi","year":"2006","journal-title":"Clin Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.vetmic.2011.06.035","article-title":"New Bruce-ladder multiplex PCR assay for the biovar typing of <italic>Brucella suis<\/italic> and the discrimination of <italic>Brucella suis<\/italic> and <italic>Brucella canis<\/italic>","volume":"154","author":"I L\u00f3pez-Go\u00f1i","year":"2011","journal-title":"Vet Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"7331","DOI":"10.1128\/AEM.00976-07","article-title":"Multiplex assay based on single-nucleotide polymorphisms for rapid identification of <italic>Brucella<\/italic> isolates at the species level","volume":"73","author":"JC Scott","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"ref26","unstructured":"Reglamento de Vigilancia de la Salud. Decreto 37306-S-2012. Gaceta (Costa Rica), No 203, (27-8-2012). 2012; Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.pgrweb.go.cr\/scij\/Busqueda\/Normativa\/Normas\/nrm_texto_completo.aspx?param1=NRTC&param2=18&nValor1=1&nValor2=73471&nValor3=90176&strTipM=TC&lResultado=180&nValor4=1&strSelect=sel\" xlink:type=\"simple\">http:\/\/www.pgrweb.go.cr\/scij\/Busqueda\/Normativa\/Normas\/nrm_texto_completo.aspx?param1=NRTC&amp;param2=18&amp;nValor1=1&amp;nValor2=73471&amp;nValor3=90176&amp;strTipM=TC&amp;lResultado=180&amp;nValor4=1&amp;strSelect=sel<\/ext-link>."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.jcpa.2009.10.017","article-title":"Pathology of striped dolphins (<italic>Stenella coeruleoalba<\/italic>) infected with <italic>Brucella ceti<\/italic>","volume":"142","author":"R Gonz\u00e1lez-Barrientos","year":"2010","journal-title":"J Comp Pathol"},{"key":"ref28","unstructured":"Cannon RM, Roe RT. Livestock disease surveys: a field manual for veterinarians. Australian Government, Australia.1982."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1136\/vr.148.18.567","article-title":"WIN EPISCOPE 2.0: improved epidemiological software for veterinary medicine","volume":"148","author":"M Thrusfield","year":"2001","journal-title":"Vet Rec"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3389\/fcimb.2012.00003","article-title":"<italic>Brucella ceti<\/italic> and brucellosis in cetaceans","volume":"2","author":"C Guzm\u00e1n-Verri","year":"2012","journal-title":"Front Cell Infect Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.vetpar.2011.05.014","article-title":"Parasites of cetaceans stranded on the Pacific coast of Costa Rica","volume":"182","author":"JB Oliveira","year":"2011","journal-title":"Vet Parasitol"},{"key":"ref32","article-title":"<italic>Brucella<\/italic> genetic variability in wildlife marine mammals populations relates to host preference and ocean distribution","author":"M Su\u00e1rez-Esquivel","year":"2017","journal-title":"Genome Biol Evol"},{"key":"ref33","first-page":"120","article-title":"La situaci\u00f3n caprina en Costa Rica: Situaci\u00f3n de la producci\u00f3n caprina en centroam\u00e9rica y Rep\u00fablica Dominicana. 1987","volume":"114","year":"1987","journal-title":"Turrialba, Costa Rica"},{"key":"ref34","unstructured":"FAOSTAT-Organizaci\u00f3n de las Naciones Unidas para la Alimentaci\u00f3n y la Agricultura. Direcci\u00f3n de Estad\u00edstica.2016: Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/faostat3.fao.org\/download\/Q\/QA\/S\" xlink:type=\"simple\">http:\/\/faostat3.fao.org\/download\/Q\/QA\/S<\/ext-link>."},{"key":"ref35","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.vetimm.2016.02.002","article-title":"A review of the basis of the immunological diagnosis of ruminant brucellosis","volume":"171","author":"MJ Ducrotoy","year":"2017","journal-title":"Vet Immunol Immunopathol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0378-1135(02)00242-0","article-title":"Brucellosis in Central America","volume":"90","author":"E Moreno","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref37","first-page":"65","article-title":"Sistemas de producci\u00f3n bufalina en Costa Rica. Cuantificaci\u00f3n de la poblaci\u00f3n y caracterizaci\u00f3n de los sistemas","volume":"31","author":"RR Rosales","year":"2007","journal-title":"Rev Agron Costarricense (Costa Rica)"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1007\/s11250-011-9846-9","article-title":"Brucellosis in domestic water buffalo (<italic>Bubalus bubalis<\/italic>) of Trinidad and Tobago with comparative epidemiology to cattle","volume":"43","author":"GT Fosgate","year":"2011","journal-title":"Trop Anim Health Prod"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1128\/IAI.74.4.2115-2120.2006","article-title":"Genetic resistance to <italic>Brucella abortus<\/italic> in the water buffalo (<italic>Bubalus bubalis<\/italic>)","volume":"74","author":"G Borriello","year":"2006","journal-title":"Infect Immun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/j.2042-3306.1973.tb03208.x","article-title":"A review of brucellosis in the horse","volume":"5","author":"HR Denny","year":"1973","journal-title":"Equine Vet J"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1186\/s12917-014-0206-7","article-title":"<italic>Brucella ceti<\/italic> infection in dolphins from the Western Mediterranean sea","volume":"10","author":"M Isidoro-Ayza","year":"2014","journal-title":"BMC Vet Res"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"517","DOI":"10.3201\/eid1403.070829","article-title":"Marine mammal Brucella genotype associated with zoonotic infection","volume":"14","author":"AM Whatmore","year":"2008","journal-title":"Emerg Infect Dis"},{"key":"ref43","first-page":"1","article-title":"Retrospective and prospective perspectives on zoonotic brucellosis","author":"E Moreno","year":"2014","journal-title":"Frontiers Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"4861","DOI":"10.1128\/IAI.00995-15","article-title":"<italic>Brucella canis<\/italic> is an intracellular pathogen inducing a lower proinflammatory response than smooth zoonotic counterparts","volume":"83","author":"C Chac\u00f3n-D\u00edaz","year":"2015","journal-title":"Infect Immun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0182644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T17:14:57Z","timestamp":1602695697000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0182644"}},"subtitle":[],"editor":[{"given":"Axel","family":"Cloeckaert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,8,9]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2017,8,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0182644","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,9]]}}}