{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:28:31Z","timestamp":1790951311080,"version":"4.1.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["11574165"],"award-info":[{"award-number":["11574165"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Program for Changjiang Scholars and Innovative Research Team in University","award":["IRT_13R29"],"award-info":[{"award-number":["IRT_13R29"]}]},{"DOI":"10.13039\/501100013314","name":"111 Project","doi-asserted-by":"crossref","award":["B07013"],"award-info":[{"award-number":["B07013"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0183114","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:23:46Z","timestamp":1502472226000},"page":"e0183114","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":45,"title":["Nucleotide transmitters ATP and ADP mediate intercellular calcium wave communication via P2Y12\/13 receptors among BV-2 microglia"],"prefix":"10.1371","volume":"12","author":[{"given":"Pengchong","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulin","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imshik","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leiting","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,8,11]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.it.2016.04.004","article-title":"Purinergic signaling during immune cell trafficking","volume":"37","author":"D Ferrari","year":"2016","journal-title":"Trends Immunol"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/nri.2016.4","article-title":"Purinergic regulation of the immune system","volume":"16","author":"C Cekic","year":"2016","journal-title":"Nat Rev Immunol"},{"issue":"5806","key":"ref3","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1126\/science.1132559","article-title":"ATP release guides neutrophil chemotaxis via P2Y<sub>2<\/sub> and A<sub>3<\/sub> receptors","volume":"314","author":"Y Chen","year":"2006","journal-title":"Science"},{"issue":"7261","key":"ref4","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1038\/nature08296","article-title":"Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance","volume":"461","author":"MR Elliott","year":"2009","journal-title":"Nature"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.neuropharm.2015.05.031","article-title":"An introduction to the roles of purinergic signalling in neurodegeneration, neuroprotection and neuroregeneration","volume":"104","author":"G Burnstock","year":"2016","journal-title":"Neuropharmacology"},{"issue":"9","key":"ref6","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1002\/glia.22693","article-title":"Inhibition of UDP\/P2Y<sub>6<\/sub> purinergic signaling prevents phagocytosis of viable neurons by activated microglia <italic>in vitro<\/italic> and <italic>in vivo<\/italic>","volume":"62","author":"JJ Neher","year":"2014","journal-title":"Glia"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1161\/ATVBAHA.115.303395","article-title":"Purinergic receptors in thrombosis and inflammation","volume":"35","author":"B Hechler","year":"2015","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1007\/s11481-016-9686-5","article-title":"Neuropharmacologic approaches to restore the brain&apos;s microenvironment","volume":"11","author":"W Li","year":"2016","journal-title":"J Neuroimmune Pharmacol"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/j.bbamcr.2010.10.018","article-title":"Microglial calcium signal acts as a rapid sensor of single neuron damage in vivo","volume":"1813","author":"G Eichhoff","year":"2011","journal-title":"Biochim Biophys Acta"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/nn1472","article-title":"ATP mediates rapid microglial response to local brain injury <italic>in vivo<\/italic>","volume":"8","author":"D Davalos","year":"2005","journal-title":"Nat Neurosci"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1085\/jgp.201010476","article-title":"Neuroglial ATP release through innexin channels controls microglial cell movement to a nerve injury","volume":"136","author":"SE Samuels","year":"2010","journal-title":"J Gen Physiol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.bbrc.2013.01.064","article-title":"Cell-cell communication induces random spikes of spontaneous calcium oscillations in multi-BV-2 microglial cells","volume":"431","author":"X Wu","year":"2013","journal-title":"Biochem Biophys Res Commun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jtbi.2014.04.024","article-title":"Modeling the functional network of primary intercellular Ca<sup>2+<\/sup> wave propagation in astrocytes and its application to study drug effects","volume":"356","author":"M Pires","year":"2014","journal-title":"J Theor Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.brainres.2008.08.085","article-title":"Sustained depolarization-induced propagation of [Ca<sup>2+<\/sup>]<sub>i<\/sub> oscillations in cultured DRG neurons: the involvement of extracellular ATP and P2Y receptor activation","volume":"1239","author":"Y Zeng","year":"2008","journal-title":"Brain Res"},{"issue":"6","key":"ref15","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/j.devcel.2012.04.012","article-title":"Long-range Ca<sup>2+<\/sup> waves transmit brain-damage signals to microglia","volume":"22","author":"D Sieger","year":"2012","journal-title":"Dev Cell"},{"issue":"8","key":"ref16","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1523\/JNEUROSCI.20-08-02835.2000","article-title":"ATP-mediated glia signaling","volume":"20","author":"ML Cotrina","year":"2000","journal-title":"J Neurosci"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1096\/fj.01-0514fje","article-title":"Astrocyte Ca<sup>2+<\/sup> waves trigger responses in microglial cells in brain slices","volume":"16","author":"CG Schipke","year":"2002","journal-title":"FASEB J"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"6383","DOI":"10.4049\/jimmunol.166.10.6383","article-title":"ATP mediates calcium signaling between astrocytes and microglial cells: modulation by IFN-gamma","volume":"166","author":"C Verderio","year":"2001","journal-title":"J Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.nbd.2016.04.008","article-title":"Protein kinase C\u03b4 upregulation in microglia drives neuroinflammatory responses and dopaminergic neurodegeneration in experimental models of Parkinson&apos;s disease","volume":"93","author":"R Gordon","year":"2016","journal-title":"Neurobiol Dis"},{"issue":"2\u20133","key":"ref20","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0014-2999(02)01756-9","article-title":"P2Y and P2X purinoceptor mediated Ca<sup>2+<\/sup> signalling in glial cell pathology in the central nervous system","volume":"447","author":"G James","year":"2002","journal-title":"Eur J Pharmacol"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.pharmthera.2005.08.014","article-title":"Pharmacological profiles of cloned mammalian P2Y-receptor subtypes","volume":"110","author":"I von K\u00fcgelgen","year":"2006","journal-title":"Pharmacol Ther"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11302-008-9106-2","article-title":"Development of selective agonists and antagonists of P2Y receptors","volume":"5","author":"KA Jacobson","year":"2009","journal-title":"Purinergic Signal"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.neuron.2004.08.015","article-title":"Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex","volume":"43","author":"TA Weissman","year":"2004","journal-title":"Neuron"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/scientificamerican1209-84","article-title":"The double life of ATP","volume":"301","author":"BS Khakh","year":"2009","journal-title":"Sci Am"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1748-1716.2010.02113.x","article-title":"Purines\u2013 80 years and very much alive","volume":"199","author":"B Fredholm","year":"2010","journal-title":"Acta Physiol (Oxf)"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/cvr\/cvs187","article-title":"Mechanisms of ATP release and signalling in the blood vessel wall","volume":"95","author":"AW Lohman","year":"2012","journal-title":"Cardiovasc Res"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s11302-012-9304-9","article-title":"Vesicular and conductive mechanisms of nucleotide release","volume":"8","author":"ER Lazarowski","year":"2012","journal-title":"Purinergic Signal"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/B978-0-12-385526-8.00008-4","article-title":"Molecular mechanisms of purine and pyrimidine nucleotide release","volume":"61","author":"ER Lazarowski","year":"2011","journal-title":"Adv Pharmacol"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ceca.2006.05.005","article-title":"Purinergic signalling and intercellular Ca<sup>2+<\/sup> wave propagation in the organ of Corti","volume":"41","author":"V Piazza","year":"2007","journal-title":"Cell Calcium"},{"issue":"7","key":"ref30","doi-asserted-by":"crossref","first-page":"799","DOI":"10.2174\/0929867322666141128163215","article-title":"Key sites for P2X receptor function and multimerization: overview of mutagenesis studies on a structural basis","volume":"22","author":"R Hausmann","year":"2015","journal-title":"Curr Med Chem"},{"issue":"Pt 2","key":"ref31","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1242\/jcs.03338","article-title":"Beta-arrestin signaling and regulation of transcription","volume":"120","author":"L Ma","year":"2007","journal-title":"J Cell Sci"},{"issue":"11","key":"ref32","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1111\/j.1538-7836.2004.00926.x","article-title":"Adenine triphosphate nucleotides are antagonists at the P2Y receptor","volume":"2","author":"G Kauffenstein","year":"2004","journal-title":"J Thromb Haemost"},{"issue":"23","key":"ref33","doi-asserted-by":"crossref","first-page":"8063","DOI":"10.1021\/jo026268l","article-title":"A general approach toward the synthesis of C-nucleoside pyrazolo [1,5-a]-1,3,5-triazines and their 3\u2032,5\u2032-bisphosphate C-nucleotide analogues as the first reported in vivo stable P2Y1-receptor antagonists","volume":"67","author":"P Raboisson","year":"2002","journal-title":"J Org Chem"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1124\/mol.53.4.727","article-title":"ATP derivatives are antagonists of the P2Y1 receptor: similarities to the platelet ADP receptor","volume":"53","author":"B Hechler","year":"1998","journal-title":"Mol Pharmacol"},{"issue":"4","key":"ref35","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S0014-5793(97)00022-7","article-title":"The P2Y1 receptor is an ADP receptor antagonized by ATP and expressed in platelets and megakaryoblastic cells","volume":"403","author":"C L\u00e9on","year":"1997","journal-title":"FEBS Lett"},{"issue":"2","key":"ref36","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1017\/S1740925X05000323","article-title":"Purinergic receptors activating rapid intracellular Ca increases in microglia","volume":"2","author":"AR Light","year":"2006","journal-title":"Neuron Glia Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3389\/fnins.2015.00148","article-title":"ATP as a multi-target danger signal in the brain","volume":"9","author":"RJ Rodrigues","year":"2015","journal-title":"Front Neurosci"},{"issue":"5","key":"ref38","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.ceca.2015.08.004","article-title":"Calcium signaling and the novel anti-proliferative effect of the UTP-sensitive P2Y<sub>11<\/sub> receptor in rat cardiac myofibroblasts","volume":"58","author":"M Certal","year":"2015","journal-title":"Cell Calcium"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"211","DOI":"10.3389\/fncel.2015.00211","article-title":"Purinergic signaling: a common pathway for neural and mesenchymal stem cell maintenance and differentiation","volume":"9","author":"F Cavaliere","year":"2015","journal-title":"Front Cell Neurosci"},{"issue":"12","key":"ref40","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1523\/JNEUROSCI.5512-08.2009","article-title":"The P2X<sub>7<\/sub> receptor drives microglial activation and proliferation: a trophic role for P2X<sub>7<\/sub>R pore","volume":"29","author":"M Monif","year":"2009","journal-title":"J Neurosci"},{"issue":"2","key":"ref41","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.4049\/jimmunol.173.2.1202","article-title":"Secretion of intracellular IL-1 receptor antagonist (type 1) is dependent on P2X<sub>7<\/sub> receptor activation","volume":"173","author":"HL Wilson","year":"2004","journal-title":"J Immunol"},{"issue":"12","key":"ref42","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1038\/nn1805","article-title":"The P2Y<sub>12<\/sub> receptor regulates microglial activation by extracellular nucleotides","volume":"9","author":"SE Haynes","year":"2006","journal-title":"Nat Neurosci"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1002\/glia.20489","article-title":"Involvement of P2X4 and P2Y12 receptors in ATP-induced microglial chemotaxis","volume":"55","author":"K Ohsawa","year":"2007","journal-title":"Glia"},{"issue":"7","key":"ref44","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1002\/jnr.21610","article-title":"Akt activation is involved in P2Y12 receptor-mediated chemotaxis of microglia","volume":"86","author":"Y Irino","year":"2008","journal-title":"J Neurosci Res"},{"issue":"8","key":"ref45","doi-asserted-by":"crossref","first-page":"650","DOI":"10.2174\/13892037113146660085","article-title":"Structure\/function relationships of phospholipases C Beta","volume":"14","author":"M Sandal","year":"2013","journal-title":"Curr Protein Pept Sci"},{"issue":"6","key":"ref46","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1523\/JNEUROSCI.21-06-01975.2001","article-title":"Extracellular ATP or ADP induce chemotaxis of cultured microglia through Gi\/o-coupled P2Y receptors","volume":"21","author":"S Honda","year":"2001","journal-title":"J Neurosci"},{"key":"ref47","first-page":"S12","article-title":"The somatotropic axis and sleep","volume":"157","author":"FJ Ob\u00e1l","year":"2001","journal-title":"Rev Neurol"},{"issue":"2","key":"ref48","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/j.1471-4159.2010.06937.x","article-title":"TGF-\u03b2 and LPS modulate ADP-induced migration of microglial cells through P2Y1 and P2Y12 receptor expression","volume":"115","author":"R De Simone","year":"2010","journal-title":"J Neurochem"},{"issue":"11","key":"ref49","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1007\/s11064-014-1426-8","article-title":"P2Y13 receptor-mediated rapid increase in intracellular calcium induced by ADP in cultured dorsal spinal cord microglia","volume":"39","author":"J Zeng","year":"2014","journal-title":"Neurochem Res"},{"issue":"4","key":"ref50","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/s11302-006-9023-1","article-title":"Different patterns of Ca2+ signals are induced by low compared to high concentrations of P2Y agonists in microglia","volume":"2","author":"S Visentin","year":"2006","journal-title":"Purinergic Signal"},{"issue":"11","key":"ref51","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1002\/glia.20692","article-title":"Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells","volume":"56","author":"Q Cao","year":"2008","journal-title":"Glia"},{"issue":"2","key":"ref52","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1111\/j.1471-4159.2008.05633.x","article-title":"Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures","volume":"107","author":"RJ Horvath","year":"2008","journal-title":"J Neurochem"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0183114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T04:38:49Z","timestamp":1589776729000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0183114"}},"subtitle":[],"editor":[{"given":"Vincenzo","family":"Lionetti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":52,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2017,8,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0183114","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}