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Patent PCT\/EP2016062383."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/S0021-9673(01)93497-1","article-title":"Gas chromatographic method for separation of nine polyhydroxy alkaloids","volume":"366","author":"RJ Nash","year":"1986","journal-title":"J Chromatogr"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1079\/BJN19870098","article-title":"Factors affecting the rate of breakdown of bacterial protein in rumen fluid","volume":"58","author":"RJ Wallace","year":"1987","journal-title":"Br J Nutr"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4612-2776-2","article-title":"The rumen protozoa","author":"AG Williams","year":"1992"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1080\/02652030400002188","article-title":"Effect and stability of gliotoxin, an Aspergillus fumigatus toxin, on in vitro rumen fermentation","volume":"21","author":"DP Morgavi","year":"2004","journal-title":"Food Addit Contam"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1194\/jlr.M700271-JLR200","article-title":"Isomers of conjugated linoleic acids are synthesized via different mechanisms in ruminal digesta and bacteria","volume":"48","author":"RJ Wallace","year":"2007","journal-title":"J Lipid Res"},{"key":"ref19","first-page":"7","article-title":"Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid","volume":"28","author":"KH Menke","year":"1988","journal-title":"Anim Res Dev"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Newbold CJ. 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