{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T01:45:55Z","timestamp":1783734355201,"version":"3.55.0"},"reference-count":26,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T00:00:00Z","timestamp":1507766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19890254"],"award-info":[{"award-number":["JP19890254"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16K18926"],"award-info":[{"award-number":["JP16K18926"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0186392","type":"journal-article","created":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T14:00:08Z","timestamp":1507816808000},"page":"e0186392","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Involvement of the Arg566 residue of Aeromonas sobria serine protease in substrate specificity"],"prefix":"10.1371","volume":"12","author":[{"given":"Hidetomo","family":"Kobayashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadamune","family":"Otsubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiteru","family":"Teraoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soshi","family":"Seike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eizo","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keinosuke","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Tsuge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyasu","family":"Yamanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,10,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/infdis\/155.3.589","article-title":"Importance of <italic>Aeromonas sobria<\/italic> in <italic>Aeromonas<\/italic> bacteremia","volume":"155","author":"JM Janda","year":"1987","journal-title":"J Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.00039-09","article-title":"The genus <italic>Aeromonas<\/italic>: taxonomy, pathogenicity, and infection","volume":"23","author":"JM Janda","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jinf.2010.12.003","article-title":"<italic>Aeromonas<\/italic> spp. clinical microbiology and disease","volume":"62","author":"JL Parker","year":"2011","journal-title":"J Infect"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1007\/s11999-008-0504-0","article-title":"Case reports: fatal necrotizing fasciitis caused by <italic>Aeromonas sobria<\/italic> in two diabetic patients","volume":"467","author":"Y-H Tsai","year":"2009","journal-title":"Clin Orthop Relat Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"631","DOI":"10.2106\/JBJS.F.00580","article-title":"Necrotizing soft-tissue infections and sepsis caused by <italic>Vibrio vulnificus<\/italic> compared with those caused by <italic>Aeromonas<\/italic> species","volume":"89","author":"Y-H Tsai","year":"2007","journal-title":"J Bone Joint Surg Am"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1093\/jac\/35.4.453","article-title":"<italic>Aeromonas<\/italic> infections and their treatment","volume":"35","author":"BL Jones","year":"1995","journal-title":"J Antimicrob Chemother"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1086\/514652","article-title":"Evolving concepts regarding the genus <italic>Aeromonas<\/italic>:an expanding Panorama of species, disease presentations, and unanswered questions","volume":"27","author":"JM Janda","year":"1998","journal-title":"Clin Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"8272","DOI":"10.1128\/JB.00621-06","article-title":"Genome Sequence of <italic>Aeromonas hydrophila<\/italic> ATCC 7966T: Jack of All Trades","volume":"188","author":"R Seshadri","year":"2006","journal-title":"J Bacteriol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.1997.tb10401.x","article-title":"Secreted enzymes of <italic>Aeromonas<\/italic>","volume":"152","author":"JM Pemberton","year":"1997","journal-title":"FEMS Microbiol Lett"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1002\/pmic.200600396","article-title":"Characterization of extracellular proteins produced by <italic>Aeromonas hydrophila<\/italic> AH-1","volume":"7","author":"HB Yu","year":"2007","journal-title":"Proteomics"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1248\/bpb.b13-00161","article-title":"Analysis of carboxy terminal domain of metalloprotease of elastolytic <italic>Aeromonas hydrophila<\/italic>","volume":"36","author":"E Takahashi","year":"2013","journal-title":"Biol Pharm Bull"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e91149","DOI":"10.1371\/journal.pone.0091149","article-title":"Properties of hemolysin and protease produced by <italic>Aeromonas trota<\/italic>","volume":"9","author":"E Takahashi","year":"2014","journal-title":"PLoS ONE"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1111\/j.1348-0421.1998.tb02343.x","article-title":"Purification and characterization of enterotoxin produced by <italic>Aeromonas sobria<\/italic>","volume":"42","author":"Y Fujii","year":"1998","journal-title":"Microbiol Immunol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"8723","DOI":"10.4049\/jimmunol.177.12.8723","article-title":"Induction of vascular leakage and blood pressure lowering through kinin release by a serine proteinase from <italic>Aeromonas sobria<\/italic>","volume":"177","author":"T Imamura","year":"2006","journal-title":"J Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3602","DOI":"10.4049\/jimmunol.181.5.3602","article-title":"Production of C5a by ASP, a serine protease released from <italic>Aeromonas sobria<\/italic>","volume":"181","author":"H Nitta","year":"2008","journal-title":"J Immunol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1111\/j.1348-0421.2000.tb02565.x","article-title":"Production of serine protease of <italic>Aeromonas sobria<\/italic> is controlled by the protein encoded by the gene lying adjacent to the 3&apos; end of the protease gene","volume":"44","author":"K Okamoto","year":"2000","journal-title":"Microbiol Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"11130","DOI":"10.1074\/jbc.M114.622852","article-title":"Structural Basis for Action of the External Chaperone for a Propeptide-deficient Serine Protease from <italic>Aeromonas sobria<\/italic>","volume":"290","author":"H Kobayashi","year":"2015","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"27655","DOI":"10.1074\/jbc.M109.006114","article-title":"Structural basis for the kexin-like serine protease from <italic>Aeromonas sobria<\/italic> as sepsis-causing factor","volume":"284","author":"H Kobayashi","year":"2009","journal-title":"J Biol Chem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1574-6968.2006.00134.x","article-title":"Cleavage specifcity of the serine protease of <italic>Aeromonas sobria<\/italic>, amemberof the kexin family of subtilases","volume":"256","author":"H Kobayashi","year":"2006","journal-title":"FEMS Microbiol Lett"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1073\/pnas.82.2.488","article-title":"Rapid and efficient site-specific mutagenesis without phenotypic selection","volume":"82","author":"TA Kunkel","year":"1985","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1093\/nar\/gnh110","article-title":"An efficient one-step site-directed and site-saturation mutagenesis protocol","volume":"32","author":"L Zheng","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.bmc.2014.01.011","article-title":"Design and synthesis of peptide-MCA substrates for a novel assay of histone methyltransferases and their inhibitors","volume":"22","author":"H Chi","year":"2014","journal-title":"Bioorg Med Chem"},{"key":"ref23","article-title":"The PyMOL Molecular Graphics System","author":"WL DeLano","year":"2002"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/pro.5560060301","article-title":"Subtilases: the superfamily of subtilisin-like serine proteases","volume":"6","author":"RJ Siezen","year":"1997","journal-title":"Protein Sci"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1021\/bi300070z","article-title":"P3-P3&apos; residues flanking scissile bonds in factor VIII modulate rates of substrate cleavage and procofactor activation by thrombin","volume":"51","author":"JL Newell-Caito","year":"2012","journal-title":"Biochemistry"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"5935","DOI":"10.1016\/j.febslet.2007.11.076","article-title":"Activation of prothrombin by ASP, a serine protease released from <italic>Aeromonas sobria<\/italic>","volume":"581","author":"H Nitta","year":"2007","journal-title":"FEBS Lett"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0186392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,4]],"date-time":"2019-10-04T11:31:59Z","timestamp":1570188719000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0186392"}},"subtitle":[],"editor":[{"given":"S. Ashraf","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,10,12]]},"references-count":26,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2017,10,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0186392","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,12]]}}}