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Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.epa.gov\/opp00001\/methods\/atmpmethods\/QC-23-01.pdf\" xlink:type=\"simple\">http:\/\/www.epa.gov\/opp00001\/methods\/atmpmethods\/QC-23-01.pdf<\/ext-link>"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0929-1393(02)00092-6","article-title":"Pathogenicity and attractiveness of entomopathogenic hyphomycete fungi to collembolans","volume":"21","author":"KM Dromph","year":"2002","journal-title":"Appl Soil Ecol"},{"key":"ref29","unstructured":"European Commission. Initial risk assessment provided by the rapporteur Member State The Netherlands for the existing active substance Metarhizium anisopliae var. \u201cBIPESCO 5\/F52\u201d [Internet]. 2008. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.efsa.europa.eu\/en\/efsajournal\/pub\/2498\" xlink:type=\"simple\">https:\/\/www.efsa.europa.eu\/en\/efsajournal\/pub\/2498<\/ext-link>"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.biocontrol.2015.10.003","article-title":"Compatibility of soil-dwelling predators and microbial agents and their efficacy in controlling soil-dwelling stages of western flower thrips <italic>Frankliniella occidentalis<\/italic>","volume":"92","author":"T Saito","year":"2016","journal-title":"Biol Control"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/jen.12229","article-title":"Non-target effects of insecticides, entomopathogenic fungi and nematodes applied against western corn rootworm larvae in maize","volume":"139","author":"D Babendreier","year":"2015","journal-title":"J Appl Entomol"},{"key":"ref32","first-page":"181","article-title":"Persistence of released <italic>Metarhizium anisopliae<\/italic> in soil and prevalence in ground and rove beetles","volume":"23","author":"S Vestergaard","year":"2000","journal-title":"Bull OILB\/SROP. 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Met 52\u00ae EC (NY Product No. 70127\u201310) [Internet]. 2012. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.dec.ny.gov\/nyspad\/products?1\" xlink:type=\"simple\">http:\/\/www.dec.ny.gov\/nyspad\/products?1<\/ext-link>"},{"key":"ref45","article-title":"Immature Insects","author":"FW Stehr","year":"1987"},{"key":"ref46","article-title":"Borror and DeLong\u2019s Introduction to the Study of Insects","author":"NF Johnson","year":"2005"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.2041-210X.2012.00190.x","article-title":"Mvabund- an R package for model-based analysis of multivariate abundance data","volume":"3","author":"Y Wang","year":"2012","journal-title":"Methods Ecol Evol"},{"key":"ref48","article-title":"R: A Language and Environment for Statistical Computing","year":"2016"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"912","DOI":"10.2307\/3803155","article-title":"Avoiding pitfalls when using information-theoretic methods","volume":"66","author":"DR Anderson","year":"2002","journal-title":"J Wildl Manage"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1198\/016214508000000021","article-title":"Penalized normal likelihood and ridge regularization of correlation and covariance matrices","volume":"103","author":"DI Warton","year":"2008","journal-title":"J Am Stat Assoc"},{"key":"ref51","first-page":"188","article-title":"Evaluating impacts using a BACI design, ratios, and a Bayesian approach with a focus on restoration. 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