{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T22:32:59Z","timestamp":1792276379406,"version":"4.4.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T00:00:00Z","timestamp":1511136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NWO-WOTRO Science for Global Development","award":["W01.65.321.00"],"award-info":[{"award-number":["W01.65.321.00"]}]},{"name":"Institutional Research Theme of the University of Pretoria on Animal Zoonotic Disease and the Third Framework Agreement Programme, Institute of Tropical Medicine, Antwerp, Belgium","award":["FA3-III"],"award-info":[{"award-number":["FA3-III"]}]},{"name":"MINECO and FEDER Plan Nacional","award":["AGL2014-56305"],"award-info":[{"award-number":["AGL2014-56305"]}]},{"name":"Erasmus Mundus Action 2 EUROSA"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0188448","type":"journal-article","created":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T18:28:28Z","timestamp":1511202508000},"page":"e0188448","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Immune response profiles of calves following vaccination with live BCG and inactivated Mycobacterium bovis vaccine candidates"],"prefix":"10.1371","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8444-6928","authenticated-orcid":true,"given":"E. M. D. L.","family":"van der Heijden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Chileshe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C. M.","family":"Vernooij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Gortazar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Juste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Sevilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Crafford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. P. M. G.","family":"Rutten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"Michel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,11,20]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.20506\/rst.20.1.1262","article-title":"Mycobacterium bovis in free-living and captive wildlife, including farmed deer","volume":"20","author":"GW de Lisle","year":"2001","journal-title":"Rev Sci Tech"},{"issue":"13","key":"ref2","doi-asserted-by":"crossref","first-page":"7877","DOI":"10.1073\/pnas.1130426100","article-title":"The complete genome sequence of Mycobacterium bovis","volume":"100","author":"T Garnier","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ref3","first-page":"583","article-title":"Improving specific disease outcomes through a One Health approach\u2014tuberculosis","volume":"33","author":"AL Michel","year":"2014","journal-title":"Rev Sci Tech"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1177\/0300985812467472","article-title":"Wildlife reservoirs of bovine tuberculosis worldwide: hosts, pathology, surveillance, and control","volume":"50","author":"SD Fitzgerald","year":"2013","journal-title":"Vet Pathol"},{"issue":"Suppl 1","key":"ref5","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/tbed.12092","article-title":"Overview of vaccination trials for control of tuberculosis in cattle, wildlife and humans","volume":"60","author":"BM Buddle","year":"2013","journal-title":"Transbound Emerg Dis"},{"issue":"5\u20136","key":"ref6","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0020-7519(03)00060-2","article-title":"Development of vaccines to control bovine tuberculosis in cattle and relationship to vaccine development for other intracellular pathogens","volume":"33","author":"BM Buddle","year":"2003","journal-title":"Int J Parasitol"},{"issue":"4","key":"ref7","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1136\/vr.102581","article-title":"Vaccination against tuberculosis in badgers and cattle: an overview of the challenges, developments and current research priorities in Great Britain","volume":"175","author":"MA Chambers","year":"2014","journal-title":"Vet Rec"},{"issue":"Suppl","key":"ref8","doi-asserted-by":"crossref","first-page":"S53","DOI":"10.1016\/j.rvsc.2014.04.015","article-title":"Vaccination of domestic animals against tuberculosis: review of progress and contributions to the field of the TBSTEP project","volume":"97","author":"HM Vordermeier","year":"2014","journal-title":"Res Vet Sci"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1128\/IAI.00287-09","article-title":"Viral booster vaccines improve Mycobacterium bovis BCG-induced protection against bovine tuberculosis","volume":"77","author":"HM Vordermeier","year":"2009","journal-title":"Infect Immun"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"e1002607","DOI":"10.1371\/journal.ppat.1002607","article-title":"Vaccines against tuberculosis: where are we and where do we need to go?","volume":"8","author":"TH Ottenhoff","year":"2012","journal-title":"PLoS Pathog"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1046\/j.1365-2567.2003.01589.x","article-title":"A DNA prime-live vaccine boost strategy in mice can augment IFN-gamma responses to mycobacterial antigens but does not increase the protective efficacy of two attenuated strains of Mycobacterium bovis against bovine tuberculosis","volume":"108","author":"MA Skinner","year":"2003","journal-title":"Immunology"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S1472-9792(03)00055-6","article-title":"Vaccination with DNA vaccines encoding MPB70 or MPB83 or a MPB70 DNA prime-protein boost does not protect cattle against bovine tuberculosis","volume":"83","author":"DN Wedlock","year":"2003","journal-title":"Tuberculosis (Edinb)"},{"issue":"8","key":"ref13","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.vaccine.2003.05.002","article-title":"A single dose of killed Mycobacterium bovis BCG in a novel class of adjuvant (Novasome\u2122) protects guinea pigs from lethal tuberculosis","volume":"22","author":"MA Chambers","year":"2004","journal-title":"Vaccine"},{"key":"ref14","unstructured":"UNAIDS. Global AIDS update [Report]. 2016. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.unaids.org\/en\/resources\/documents\/2016\/Global-AIDS-update-2016\" xlink:type=\"simple\">http:\/\/www.unaids.org\/en\/resources\/documents\/2016\/Global-AIDS-update-2016<\/ext-link>."},{"issue":"3\u20134","key":"ref15","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.vetmic.2009.08.029","article-title":"Mycobacterium bovis at the animal-human interface: a problem, or not?","volume":"140","author":"AL Michel","year":"2010","journal-title":"Vet Microbiol"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.vaccine.2007.11.005","article-title":"Evidence for enhanced central memory priming by live Mycobacterium bovis BCG vaccine in comparison with killed BCG formulations","volume":"26","author":"AO Whelan","year":"2008","journal-title":"Vaccine"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"e24905","DOI":"10.1371\/journal.pone.0024905","article-title":"Protection against tuberculosis in Eurasian wild boar vaccinated with heat-inactivated Mycobacterium bovis","volume":"6","author":"JM Garrido","year":"2011","journal-title":"PloS One"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetimm.2016.03.003","article-title":"Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin","volume":"172","author":"V Lopez","year":"2016","journal-title":"Vet Immunol Immunopathol"},{"issue":"11","key":"ref19","doi-asserted-by":"crossref","first-page":"e0165607","DOI":"10.1371\/journal.pone.0165607","article-title":"Increased Lytic Efficiency of Bovine Macrophages Trained with Killed Mycobacteria","volume":"11","author":"RA Juste","year":"2016","journal-title":"PLoS One"},{"issue":"2\u20133","key":"ref20","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.prevetmed.2010.10.016","article-title":"Approaches towards optimising the gamma interferon assay for diagnosing Mycobacterium bovis infection in African buffalo (Syncerus caffer)","volume":"98","author":"AL Michel","year":"2011","journal-title":"Prev Vet Med"},{"key":"ref21","article-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals","year":"2014"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1128\/CVI.00024-12","article-title":"Improved skin test for differential diagnosis of bovine tuberculosis by the addition of Rv3020c-derived peptides","volume":"19","author":"GJ Jones","year":"2012","journal-title":"Clin Vaccine Immunol"},{"key":"ref23","article-title":"Development of a BCG challenge model for the testing of vaccine candidates against tuberculosis in cattle","author":"B Villarreal-Ramos","year":"2014","journal-title":"Vaccine"},{"issue":"7","key":"ref24","first-page":"818","article-title":"Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference","volume":"10","author":"RM Warren","year":"2006","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref25","article-title":"R: A Language and Environment for Statistical Computing","year":"2016"},{"key":"ref26","first-page":"ed2016","article-title":"nlme: Linear and Nonlinear Mixed Effects Models","volume":"1\u2013128","author":"J Pinheiro","journal-title":"R package version 3"},{"key":"ref27","first-page":"ed2016","article-title":"dglm: Double Generalized Linear Models","author":"PK Dunn","journal-title":"R package version 1.8.3"},{"issue":"5","key":"ref28","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/jb.178.5.1274-1282.1996","article-title":"Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis","volume":"178","author":"GG Mahairas","year":"1996","journal-title":"J Bacteriol"},{"issue":"Pt 11","key":"ref29","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1099\/00221287-144-11-3195","article-title":"A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10)","volume":"144","author":"FX Berthet","year":"1998","journal-title":"Microbiology"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"e98048","DOI":"10.1371\/journal.pone.0098048","article-title":"Oral vaccination with heat inactivated Mycobacterium bovis activates the complement system to protect against tuberculosis","volume":"9","author":"B Beltran-Beck","year":"2014","journal-title":"PLoS One"},{"issue":"11\u201312","key":"ref31","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1016\/j.vaccine.2003.10.016","article-title":"Immunization with heat-killed Mycobacterium bovis bacille Calmette-Guerin (BCG) in Eurocine L3 adjuvant protects against tuberculosis","volume":"22","author":"M Haile","year":"2004","journal-title":"Vaccine"},{"issue":"6","key":"ref32","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1128\/IAI.01121-12","article-title":"Postexposure subunit vaccination against chronic enteric mycobacterial infection in a natural host","volume":"81","author":"W Santema","year":"2013","journal-title":"Infect Immun"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1365-2249.2005.02668.x","article-title":"Vaccination of neonatal calves with Mycobacterium bovis BCG induces protection against intranasal challenge with virulent M. bovis","volume":"139","author":"JC Hope","year":"2005","journal-title":"Clin Exp Immunol"},{"issue":"5","key":"ref34","first-page":"822","article-title":"Local cellular immunity in tuberculous pleurisy","volume":"126","author":"K Shimokata","year":"1982","journal-title":"Am Rev Respir Dis"},{"issue":"4","key":"ref35","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.4049\/jimmunol.142.4.1114","article-title":"Compartmentalization of a CD4+ T lymphocyte subpopulation in tuberculous pleuritis","volume":"142","author":"PF Barnes","year":"1989","journal-title":"J Immunol"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4049\/jimmunol.145.1.149","article-title":"Local production of tumor necrosis factor and IFN-gamma in tuberculous pleuritis","volume":"145","author":"PF Barnes","year":"1990","journal-title":"J Immunol"},{"issue":"1\u20132","key":"ref37","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.vetimm.2006.03.010","article-title":"Development of cattle TB vaccines in the UK","volume":"112","author":"M Vordermeier","year":"2006","journal-title":"Vet Immunol Immunopathol"},{"issue":"14","key":"ref38","doi-asserted-by":"crossref","first-page":"5730","DOI":"10.1073\/pnas.1015153108","article-title":"Rv3615c is a highly immunodominant RD1 (Region of Difference 1)-dependent secreted antigen specific for Mycobacterium tuberculosis infection","volume":"108","author":"KA Millington","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1046\/j.1365-2958.2002.03237.x","article-title":"Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacterium microti","volume":"46","author":"AS Pym","year":"2002","journal-title":"Mol Microbiol"},{"issue":"9","key":"ref40","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1128\/CVI.00261-10","article-title":"Screening of predicted secreted antigens from Mycobacterium bovis identifies potential novel differential diagnostic reagents","volume":"17","author":"GJ Jones","year":"2010","journal-title":"Clin Vaccine Immunol"},{"issue":"2","key":"ref41","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.rvsc.2005.11.005","article-title":"Ante mortem diagnosis of tuberculosis in cattle: a review of the tuberculin tests, gamma-interferon assay and other ancillary diagnostic techniques","volume":"81","author":"R de la Rua-Domenech","year":"2006","journal-title":"Res Vet Sci"},{"issue":"1\u20133","key":"ref42","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S1472-9792(02)00062-8","article-title":"Use of the bovine model of tuberculosis for the development of improved vaccines and diagnostics","volume":"83","author":"RG Hewinson","year":"2003","journal-title":"Tuberculosis (Edinb)"},{"issue":"2\u20134","key":"ref43","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.vetmic.2005.11.027","article-title":"Progress in the development of tuberculosis vaccines for cattle and wildlife","volume":"112","author":"BM Buddle","year":"2006","journal-title":"Vet Microbiol"},{"issue":"7\u20138","key":"ref44","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1016\/S0264-410X(01)00436-4","article-title":"Influence of sensitisation to environmental mycobacteria on subsequent vaccination against bovine tuberculosis","volume":"20","author":"BM Buddle","year":"2002","journal-title":"Vaccine"},{"issue":"8986","key":"ref45","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0140-6736(95)92348-9","article-title":"Variation in protection by BCG: implications of and for heterologous immunity","volume":"346","author":"PE Fine","year":"1995","journal-title":"Lancet"},{"key":"ref46","article-title":"The occurrence and molecular characterization of non-tuberculous mycobacteria in cattle, African buffalo (Syncerus caffer) and their environments in South Africa and genomic characterization and proteomic comparison with Mycobacterium bovis","author":"N Gcebe","year":"2015"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0188448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T19:24:42Z","timestamp":1693250682000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0188448"}},"subtitle":[],"editor":[{"given":"Ann","family":"Rawkins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,11,20]]},"references-count":46,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0188448","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,20]]}}}