{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:10:24Z","timestamp":1784074224815,"version":"3.55.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T00:00:00Z","timestamp":1511222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AGL2011-30563-C03-03"],"award-info":[{"award-number":["AGL2011-30563-C03-03"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008425","name":"Conseller\u00eda de Cultura, Educaci\u00f3n e Ordenaci\u00f3n Universitaria, Xunta de Galicia","doi-asserted-by":"publisher","award":["GPC2014\/058"],"award-info":[{"award-number":["GPC2014\/058"]}],"id":[{"id":"10.13039\/501100008425","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Network of BiomedicalResearch on Tropical Diseases (RICET)","award":["RD12\/0018\/0013"],"award-info":[{"award-number":["RD12\/0018\/0013"]}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AGL2011-30563-C03-02"],"award-info":[{"award-number":["AGL2011-30563-C03-02"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AGL2011-30563-C03-01"],"award-info":[{"award-number":["AGL2011-30563-C03-01"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0188520","type":"journal-article","created":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T18:38:50Z","timestamp":1511289530000},"page":"e0188520","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Fasciola spp: Mapping of the MF6 epitope and antigenic analysis of the MF6p\/HDM family of heme-binding proteins"],"prefix":"10.1371","volume":"12","author":[{"given":"Victoria","family":"Mart\u00ednez-Sern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Perteguer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Mezo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Gonz\u00e1lez-Warleta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"G\u00e1rate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Adela","family":"Valero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencio M.","family":"Ubeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,11,21]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.3201\/eid1110.050614","article-title":"Emerging foodborne trematodiasis","volume":"11","author":"J Keiser","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.ijpara.2005.07.010","article-title":"Fascioliasis and other plant-borne trematode zoonoses","volume":"35","author":"S Mas-Coma","year":"2005","journal-title":"Int J Parasitol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/978-1-4939-0915-5_4","article-title":"Fascioliasis","volume":"766","author":"S Mas-Coma","year":"2014","journal-title":"Adv Exp Med Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/trstmh\/trv110","article-title":"Higher physiopathogenicity by <italic>Fasciola gigantica<\/italic> than by the genetically close <italic>F<\/italic>. <italic>hepatica<\/italic>: experimental long-term follow-up of biochemical markers","volume":"110","author":"MA Valero","year":"2016","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.prevetmed.2006.09.010","article-title":"Associations between anti-<italic>Fasciola hepatica<\/italic> antibody levels in bulk-tank milk samples and production parameters in dairy herds","volume":"78","author":"J Charlier","year":"2007","journal-title":"Prev Vet Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.vetpar.2011.03.009","article-title":"Association between anti-<italic>F<\/italic>. <italic>hepatica<\/italic> antibody levels in milk and production losses in dairy cows","volume":"180","author":"M Mezo","year":"2011","journal-title":"Vet Parasitol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1136\/vr.157.7.188","article-title":"Estimating the financial losses due to bovine fasciolosis in Switzerland","volume":"157","author":"G Schweizer","year":"2005","journal-title":"Vet Rec"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.vetpar.2009.09.040","article-title":"Measurement of antibodies to gastrointestinal nematodes and liver fluke in meat juice of beef cattle and associations with carcass parameters","volume":"166","author":"J Charlier","year":"2009","journal-title":"Vet Parasitol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.tmaid.2014.09.006","article-title":"Fascioliasis: a worldwide parasitic disease of importance in travel medicine","volume":"12","author":"K Ashrafi","year":"2014","journal-title":"Travel Med Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1645\/GE-192R","article-title":"An ultrasensitive capture ELISA for detection of <italic>Fasciola hepatica<\/italic> coproantigens in sheep and cattle using a new monoclonal antibody (MM3)","volume":"90","author":"M Mezo","year":"2004","journal-title":"J Parasitol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1645\/GE-925R.1","article-title":"The use of MM3 monoclonal antibodies for the early immunodiagnosis of ovine fascioliasis","volume":"93","author":"M Mezo","year":"2007","journal-title":"J Parasitol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1017\/S0031182014000869","article-title":"Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario","volume":"141","author":"S Mas-Coma","year":"2014","journal-title":"Parasitology"},{"key":"ref13","first-page":"277","article-title":"Fasciolosis","author":"AGM Tielens","year":"1999"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1128\/MMBR.05024-11","article-title":"Biochemistry and evolution of anaerobic energy metabolism in eukaryotes","volume":"76","author":"M M\u00fcller","year":"2012","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13059-015-0632-2","article-title":"The <italic>Fasciola hepatica<\/italic> genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution","volume":"16","author":"K Cwiklinski","year":"2015","journal-title":"Genome Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.tibs.2008.09.001","article-title":"Helminth pathogen cathepsin proteases: it&apos;s a family affair","volume":"33","author":"MW Robinson","year":"2008","journal-title":"Trends Biochem Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"17038","DOI":"10.1074\/jbc.M308831200","article-title":"Cathepsin L1, the major protease involved in liver fluke (<italic>Fasciola hepatica<\/italic>) virulence: propetide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells","volume":"279","author":"PR Collins","year":"2004","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.molbiopara.2011.06.003","article-title":"Molecular and immunological characterization of <italic>Fasciola<\/italic> antigens recognized by the MM3 monoclonal antibody","volume":"179","author":"L Mui\u00f1o","year":"2011","journal-title":"Mol Biochem Parasitol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/S0020-7519(03)00171-1","article-title":"<italic>Fasciola hepatica<\/italic> cathepsin L-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines","volume":"33","author":"JP Dalton","year":"2003","journal-title":"Int J Parasitol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"32796","DOI":"10.1038\/srep32796","article-title":"Across intra-mammalian stages of the liver fluke <italic>Fasciola hepatica<\/italic>: a proteomic study","volume":"6","author":"LS Di Maggio","year":"2016","journal-title":"Sci Rep"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1074\/mcp.M900045-MCP200","article-title":"An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen <italic>Fasciola hepatica<\/italic>: proteins associated with invasion and infection of the mammalian host","volume":"8","author":"MW Robinson","year":"2009","journal-title":"Mol Cell Proteomics"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e1002042","DOI":"10.1371\/journal.ppat.1002042","article-title":"A family of helminth molecules that modulate innate cell responses via molecular mimicry of host antimicrobial peptides","volume":"7","author":"MW Robinson","year":"2011","journal-title":"PLoS Pathog"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1074\/jbc.M113.499517","article-title":"The MF6p\/FhHDM-1 major antigen secreted by the trematode parasite <italic>Fasciola hepatica<\/italic> is a heme-binding protein","volume":"289","author":"V Mart\u00ednez-Sern\u00e1ndez","year":"2014","journal-title":"J Biol Chem"},{"key":"ref24","article-title":"Delineating distinct heme-scavenging and binding functions of domains in MF6p\/HDM proteins from parasitic flatworms","author":"V Mart\u00ednez-Sern\u00e1ndez","year":"2017","journal-title":"J Biol Chem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1096\/fj.201500093R","article-title":"The immune modulatory peptide FhHDM-1 secreted by the helminth <italic>Fasciola hepatica<\/italic> prevents NLRP3 inflammasome activation by inhibiting endolysosomal acidification in macrophages","volume":"31","author":"R Alvarado","year":"2017","journal-title":"FASEB J"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1645\/GE-74RI.1","article-title":"Optimized serodiagnosis of sheep fascioliasis by Fast-D protein liquid chromatography fractionation of <italic>Fasciola hepatica<\/italic> excretory-secretory antigens","volume":"89","author":"M Mezo","year":"2003","journal-title":"J Parasitol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.vetpar.2008.10.014","article-title":"MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with <italic>Fasciola hepatica<\/italic> and <italic>F<\/italic>. <italic>gigantica<\/italic>","volume":"159","author":"MA Valero","year":"2009","journal-title":"Vet Parasitol"},{"key":"ref28","author":"J Sambrook","year":"2001"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"74","author":"F Sanger","year":"1977","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"ref30","doi-asserted-by":"crossref","first-page":"e0004872","DOI":"10.1371\/journal.pntd.0004872","article-title":"Rapid enhanced MM3-COPRO ELISA for detection of <italic>Fasciola<\/italic> coproantigens","volume":"10","author":"V Mart\u00ednez-Sern\u00e1ndez","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"W389","DOI":"10.1093\/nar\/gkv332","article-title":"JPred4: a protein secondary structure prediction server","volume":"43","author":"A Drozdetskiy","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1002\/prot.24065","article-title":"Ab initio protein structure assembly using continuous structure fragments and optimized knowledge-based force field","volume":"80","author":"D Xu","year":"2012","journal-title":"Proteins"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1749-6632.2009.05127.x","article-title":"Epitope specificity and significance in systemic autoimmune diseases","volume":"1183","author":"M Mahler","year":"2010","journal-title":"Ann N Y Acad Sci"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.molimm.2012.03.030","article-title":"An analysis of B-cell epitope discontinuity","volume":"51","author":"GN Sivalingam","year":"2012","journal-title":"Mol Immunol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.molimm.2004.03.032","article-title":"Minor interspecies variations in the sequence of the gp53 TSL-1 antigen of <italic>Trichinella<\/italic> define species-specific immunodominant epitopes","volume":"41","author":"MJ Perteguer","year":"2004","journal-title":"Mol Immunol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e0170530","DOI":"10.1371\/journal.pone.0170530","article-title":"Peptide targeted by human antibodies associated with HIV vaccine-associated protection assumes a dynamic \u03b1-Helical structure","volume":"12","author":"MA Aiyegbo","year":"2017","journal-title":"PLoS One"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1128\/CDLI.11.4.814-817.2004","article-title":"Evaluation of <italic>Clonorchis sinensis<\/italic> recombinant 7-kilodalton antigen for serodiagnosis of clonorchiasis","volume":"11","author":"QP Zhao","year":"2004","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1128\/IAI.64.12.5066-5074.1996","article-title":"Induction of protective immunity in cattle against infection with <italic>Fasciola hepatica<\/italic> by vaccination with cathepsin L proteinases and with hemoglobin","volume":"64","author":"JP Dalton","year":"1996","journal-title":"Infect Immun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e2307","DOI":"10.1371\/journal.pntd.0002307","article-title":"Cathelicidin-like helminth defence molecules (HDMs): absence of cytotoxic, anti-microbial and anti-protozoan activities imply a specific adaptation to immune modulation.","volume":"7","author":"K Thivierge","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3389\/fmicb.2013.00296","article-title":"Helminth defence molecules-immunomodulators designed by parasites!","volume":"4","author":"MW Robinson","year":"2013","journal-title":"Front Microbiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0188520","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T07:38:51Z","timestamp":1603352331000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0188520"}},"subtitle":[],"editor":[{"given":"Dhammika Nanda","family":"Atapattu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,11,21]]},"references-count":41,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0188520","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,21]]}}}