{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T23:16:24Z","timestamp":1772925384243,"version":"3.50.1"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Colciencias","doi-asserted-by":"crossref","award":["221356933625"],"award-info":[{"award-number":["221356933625"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008793","name":"Universidad del Rosario","doi-asserted-by":"publisher","award":["DVG154"],"award-info":[{"award-number":["DVG154"]}],"id":[{"id":"10.13039\/501100008793","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","award":["Sustainability Strategy Program 2016-2017"],"award-info":[{"award-number":["Sustainability Strategy Program 2016-2017"]}],"id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0190311","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T13:34:10Z","timestamp":1514554450000},"page":"e0190311","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Standardization and validation of real time PCR assays for the diagnosis of histoplasmosis using three molecular targets in an animal model"],"prefix":"10.1371","volume":"12","author":[{"given":"Luisa F.","family":"L\u00f3pez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar O.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego H.","family":"C\u00e1ceres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngela M.","family":"Tob\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Loparev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Clay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Chiller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Litvintseva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lalitha","family":"Gade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngel","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1924","authenticated-orcid":true,"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,12,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.idc.2015.10.009","article-title":"Histoplasmosis","volume":"30","author":"J Wheat","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref2","first-page":"2949","article-title":"Principles and practice of infectious diseases","author":"GS Deepe","year":"2015"},{"key":"ref3","first-page":"2305","article-title":"Enfermedades Infecciosas de Homo sapiens","author":"BL G\u00f3mez","year":"2014"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ccm.2009.02.002","article-title":"Histoplasmosis","volume":"30","author":"CA Kauffman","year":"2009","journal-title":"Clin. Chest. Med."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1128\/CVI.00066-09","article-title":"Development and evaluation of an enzyme-linked immunosorbent assay to detect <italic>Histoplasma capsulatum<\/italic> antigenuria in immunocompromised patients","volume":"16","author":"C Scheel","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1093\/cid\/cir435","article-title":"A multicenter evaluation of tests for diagnosis of histoplasmosis","volume":"53","author":"CA Hage","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"ref7","first-page":"321","article-title":"Essentials of Clinical Mycology","author":"C Kauffman","year":"2011"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"344","DOI":"10.7705\/biomedica.v31i3.348","article-title":"Histoplasmosis: Results of the Colombian National Survey, 1992\u20132008","volume":"31","author":"M Arango","year":"2011","journal-title":"Biom\u00e9dica"},{"issue":"12","key":"ref9","doi-asserted-by":"crossref","first-page":"4095","DOI":"10.1128\/JCM.02298-13","article-title":"Evaluation of commercially available reagents for the diagnosis of Histoplasmosis infection in immunocompromised patients","volume":"51","author":"X Zhang","year":"2013","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"ref10","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1128\/CVI.00101-14","article-title":"Validation of an Enzyme-Linked Immunosorbent Assay That Detects <italic>Histoplasma capsulatum<\/italic> Antigenuria in Colombian Patients with AIDS for Diagnosis and Follow-Up during Therapy","volume":"21","author":"D C\u00e1ceres","year":"2014","journal-title":"Clin. Vaccine Immunol."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"94","DOI":"10.3390\/jof1020094","article-title":"Histoplasmosis and Blastomycosis in Solid Organ Transplant Recipients","volume":"1","author":"C Kauffman","year":"2015","journal-title":"J. Fungi."},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1128\/JCM.39.7.2640-2641.2001","article-title":"Rapid diagnosis of <italic>Histoplasma capsulatum<\/italic> endocarditis using the AccuProbe on an excised valve","volume":"39","author":"RF Chemaly","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.diagmicrobio.2009.10.010","article-title":"Detection of <italic>Histoplasma capsulatum<\/italic> DNA in human samples by real-time polymerase chain reaction","volume":"66","author":"S Simon","year":"2010","journal-title":"Diag. Microbiol. Infect. Dis."},{"issue":"11","key":"ref14","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/s10096-002-0833-y","article-title":"Rapid PCR based diagnosis of disseminated histoplasmosis in an AIDS patient","volume":"21","author":"V Rickerts","year":"2002","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.diagmicrobio.2007.03.008","article-title":"Histoplasmosis diagnosis using a polymerase chain reaction method. Application on human samples in French Guiana, South America","volume":"58","author":"D Moubon","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref16","first-page":"20","article-title":"Diagn\u00f3stico molecular de histoplasmosis humana en muestras de sangre entera","volume":"41","author":"AI Toranzo","year":"2009","journal-title":"Rev. Argent. Microbiol."},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.riam.2013.03.004","article-title":"Comparison of PCR protocols for detecting <italic>Histoplasma capsulatum<\/italic> DNA through a multicenter study","volume":"30","author":"MJ Buitrago","year":"2013","journal-title":"Rev. Iberoam. Micol."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1006\/bbrc.1998.9894","article-title":"An homologue of the human 100-kDa protein (p100) is differentially expressed by <italic>Histoplasma capsulatum<\/italic> during infection of murine macrophages","volume":"254","author":"A Porta","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"5","key":"ref19","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1128\/JCM.40.5.1644-1647.2002","article-title":"Evaluation of two nested PCR assays for detection of <italic>Histoplasma capsulatum<\/italic> DNA in human tissue","volume":"40","author":"R Bialek","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref20","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1128\/CVI.00332-09","article-title":"Validation and clinical application of a molecular methods for identification of <italic>Histoplasma capsulatum<\/italic> in human specimens in Colombia, South America","volume":"17","author":"C Mu\u00f1oz","year":"2010","journal-title":"Clin. Vacc. Immunol."},{"issue":"10","key":"ref21","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1128\/JCM.01705-12","article-title":"Applying a Real-Time PCR Assay for <italic>Histoplasma capsulatum<\/italic> to Clinically Relevant Formalin-Fixed Paraffin-Embedded Human Tissue","volume":"50","author":"S Koepsell","year":"2012","journal-title":"J. Clin. Microb."},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1128\/JCM.02739-13","article-title":"Development of a Loop-Mediated Isothermal Amplification Method for Detection of <italic>Histoplasma capsulatum<\/italic> DNA in Clinical Samples","volume":"52","author":"C Scheel","year":"2014","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1128\/JCM.39.4.1506-1509.2001","article-title":"Diagnosis and monitoring of murine histoplasmosis by a nested PCR assay","volume":"39","author":"R Bialek","year":"2001","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"ref24","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1309\/MH5B-GAQ2-KY19-FT7P","article-title":"Comparison of staining methods and a nested PCR assay to detect <italic>Histoplasma capsulatum<\/italic> in tissue sections","volume":"117","author":"R Bialek","year":"2002","journal-title":"Am. J. Clin. Pathol."},{"issue":"4","key":"ref25","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1128\/JCM.41.4.1753-1755.2003","article-title":"Molecular detection of <italic>Histoplasma capsulatum var<\/italic>. <italic>capsulatum<\/italic> in human clinical samples","volume":"41","author":"A Bracca","year":"2003","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/JCM.41.2.535-539.2003","article-title":"PCR assay for identification of <italic>Histoplasma capsulatum<\/italic> based on the nucleotide sequence of the M antigen","volume":"41","author":"HL Guedes","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1086\/339824","article-title":"Polymerase Chain Reaction Detection of <italic>Aspergillus<\/italic> DNA in Experimental Models of Invasive Aspergillosis","volume":"185","author":"J Loeffler","year":"2002","journal-title":"J. Infect. Dis."},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1128\/JCM.01767-09","article-title":"<italic>Aspergillus<\/italic> PCR: One Step Closer to Standardization","volume":"48","author":"L White","year":"2010","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"ref29","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1128\/JCM.00905-15","article-title":"Clinical Performance of <italic>Aspergillus<\/italic> PCR for Testing Serum and Plasma: a Study by the European <italic>Aspergillus<\/italic> PCR Initiative","volume":"53","author":"L White","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref30","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1046\/j.1365-294X.2003.01995.x","article-title":"Phylogeography of the fungal pathogen <italic>Histoplasma capsulatum<\/italic>","volume":"12","author":"T Kasuga","year":"2003","journal-title":"Mol. Ecol."},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/3183285","article-title":"Depletion of Neutrophils Exacerbates the Early Inflammatory Immune Response in Lungs of Mice Infected with <italic>Paracoccidioides brasiliensis<\/italic>","volume":"2016","author":"PA Pino-Tamayo","year":"2016","journal-title":"Mediators of Inflammation"},{"issue":"10","key":"ref32","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1128\/JCM.00046-08","article-title":"Development and Validation of a Quantitative PCR Assay for Diagnosis of Scedosporiosis","volume":"46","author":"M Castelli","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"ref33","first-page":"1","article-title":"Depletion of Neutrophils Promotes the Resolution of Pulmonary Inflammation and Fibrosis in Mice Infected with <italic>Paracoccidioides brasiliensis<\/italic>","volume":"11","author":"JD Puerta-Arias","year":"2016","journal-title":"PLOS ONE"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1128\/JCM.00459-10","article-title":"Improving Molecular Detection of Fungal DNA in Formalin-Fixed Paraffin-Embedded Tissues: Comparison of Five Tissue DNA Extraction Methods Using Panfungal PCR","volume":"48","author":"C Mu\u00f1oz -Cadavid","year":"2010","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"ref35","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3109\/13693786.2011.604047","article-title":"Detection of invasive infection caused by <italic>Fusarium solani<\/italic> and non-<italic>Fusarium solani<\/italic> species using a duplex quantitative PCR-based assay in a murine model of fusariosis","volume":"50","author":"L Bernal-Mart\u00ednez","year":"2012","journal-title":"Medical Mycology"},{"issue":"3\u20134","key":"ref36","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.ijmm.2014.03.001","article-title":"Development of cycling probe-based real-time PCR system to detect <italic>Fusarium<\/italic> species and <italic>Fusarium solani<\/italic> species complex (FSSC)","volume":"304","author":"Y Muraosa","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"ref37","first-page":"569","article-title":"Manual of Molecular and Clinical Laboratory Immunology","author":"MD Lindsley","year":"2006"},{"issue":"1","key":"ref38","first-page":"51","article-title":"Comparison of PCR to histology for the diagnosis of invasive candidiasis in a murine model. Scand","volume":"33","author":"Z Hussain","year":"2001","journal-title":"J. Infect. Dis."},{"issue":"12","key":"ref39","doi-asserted-by":"crossref","first-page":"4150","DOI":"10.1128\/JCM.00570-11","article-title":"Molecular detection and species-specific identification of medically important <italic>Aspergillus<\/italic> species by real-time PCR in experimental invasive pulmonary aspergillosis","volume":"49","author":"TJ Walsh","year":"2011","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"ref40","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1007\/s10096-012-1561-6","article-title":"Evaluation of a digital microfluidic real-time PCR platform to detect DNA of <italic>Candida albicans<\/italic> in blood","volume":"31","author":"WA Schell","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"6","key":"ref41","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1128\/JCM.02319-09","article-title":"Molecular Detection and Identification of Zygomycetes Species from Paraffin-Embedded Tissues in a Murine Model of Disseminated Zygomycosis: a Collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study Group (EFISG) Evaluation","volume":"48","author":"E Dannaoui","year":"2010","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"ref42","first-page":"76","article-title":"Improved PCR amplification for molecular analysis using DNA from long-term preserved formalin-fixed, paraffin-embedded lung cancer tissue specimens","volume":"6","author":"M Taga","year":"2013","journal-title":"Int. J. Clin. Exp. Pathol."},{"issue":"3","key":"ref43","first-page":"269","article-title":"Extraction and amplification of DNA from formalin-fixed, paraffin-embedded tissues. Appl. Immunohistochem","volume":"10","author":"L Wu","year":"2002","journal-title":"Mol. Morphol."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0190311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:37:32Z","timestamp":1540341452000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0190311"}},"subtitle":[],"editor":[{"given":"Ulrich","family":"Melcher","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,12,29]]},"references-count":43,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2017,12,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0190311","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,29]]}}}