{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:00:16Z","timestamp":1787022016574,"version":"build-2736575974"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T00:00:00Z","timestamp":1516233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006751","name":"U.S. Army","doi-asserted-by":"publisher","award":["W81XWH-11-C-0008"],"award-info":[{"award-number":["W81XWH-11-C-0008"]}],"id":[{"id":"10.13039\/100006751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL117329"],"award-info":[{"award-number":["R01HL117329"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0190827","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T13:37:04Z","timestamp":1516282624000},"page":"e0190827","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["A portable system for processing donated whole blood into high quality components without centrifugation"],"prefix":"10.1371","volume":"13","author":[{"given":"Sean C.","family":"Gifford","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Briony C.","family":"Strachan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"V\u00f6r\u00f6s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kian","family":"Torabian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor A.","family":"Tomasino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary D.","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Lichtiger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fleur M.","family":"Aung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8203-8365","authenticated-orcid":true,"given":"Sergey S.","family":"Shevkoplyas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,1,18]]},"reference":[{"key":"ref1","article-title":"Technical Manual","author":"MK Fung","year":"2014"},{"key":"ref2","unstructured":"Medicines EDftQo. HealthCare: Guide to the Preparation, Use and Quality Assurance of blood Components. The European Union: EDQM. 2013."},{"key":"ref3","unstructured":"Organization WWH. Blood safety and availability Fact sheet N279. 2012 June 2012. Report No."},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Whitaker BI, Rajbhandary S, Harris A. The 2013 AABB Blood Collection, Utilization, and Patient Blood Management Survey Report: AABB; 2015.","DOI":"10.1111\/trf.13676"},{"key":"ref5","article-title":"Physical properties of tissues: a comprehensive reference book","author":"FA Duck","year":"2013"},{"issue":"Suppl 1","key":"ref6","doi-asserted-by":"crossref","first-page":"1S","DOI":"10.1111\/j.1537-2995.2010.02956.x","article-title":"The overnight warm hold of whole blood before processing into blood components","volume":"51","author":"JR Hess","year":"2011","journal-title":"Transfusion"},{"key":"ref7","article-title":"Standards for blood banks and transfusion services","author":"P Ooley","year":"2015"},{"key":"ref8","first-page":"103","article-title":"Operations research and health care","author":"WP Pierskalla","year":"2005"},{"issue":"11","key":"ref9","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1046\/j.1537-2995.2003.00576.x","article-title":"Blood use in war and disaster: lessons from the past century","volume":"43","author":"JR Hess","year":"2003","journal-title":"Transfusion"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.transci.2016.09.007","article-title":"Blood transfusion service in disasters","volume":"55","author":"HA Zaheer","year":"2016","journal-title":"Transfusion and Apheresis Science"},{"issue":"10","key":"ref11","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1111\/trf.12660","article-title":"Randomized study of washing 40- to 42-day-stored red blood cells","volume":"54","author":"E Bennett-Guerrero","year":"2014","journal-title":"Transfusion"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"E146","DOI":"10.21037\/jtd.2017.02.03","article-title":"Effect of "old" versus "fresh" transfused red blood cells on patients\u2019 outcome: probably more complex than appears","volume":"9","author":"O Garraud","year":"2017","journal-title":"Journal of thoracic disease"},{"issue":"1","key":"ref13","first-page":"98","article-title":"Clinical trials in Transfusion Medicine and hemotherapy: Worth moving forward in evaluating \u2018fresh\u2019 versus \u2018old\u2019 blood cell components?","volume":"56","author":"O Garraud","year":"2017","journal-title":"Transfusion and apheresis science: official journal of the World Apheresis Association: official journal of the European Society for Haemapheresis"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/vox.12130","article-title":"Measures of stored red blood cell quality","volume":"107","author":"JR Hess","year":"2014","journal-title":"Vox sanguinis"},{"issue":"14","key":"ref15","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1182\/blood-2014-11-567750","article-title":"Established and theoretical factors to consider in assessing the red cell storage lesion","volume":"125","author":"JC Zimring","year":"2015","journal-title":"Blood"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/vox.12441","article-title":"Perspectives on the impact of storage duration on blood quality and transfusion outcomes","volume":"111","author":"JD Roback","year":"2016","journal-title":"Vox sanguinis"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1172\/JCI90837","article-title":"Prolonged red cell storage before transfusion increases extravascular hemolysis","volume":"127","author":"F Rapido","year":"2017","journal-title":"The Journal of clinical investigation"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"529","DOI":"10.4103\/0019-5049.144647","article-title":"Overview of blood components and their preparation","volume":"58","author":"D Basu","year":"2014","journal-title":"Indian Journal of Anaesthesia"},{"issue":"2","key":"ref19","first-page":"97","article-title":"Preparation, storage and quality control of platelet concentrates","volume":"41","author":"N Tynngard","year":"2009","journal-title":"Transfusion and apheresis science: official journal of the World Apheresis Association: official journal of the European Society for Haemapheresis"},{"issue":"12","key":"ref20","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1111\/j.1537-2995.2007.01456.x","article-title":"Quality and stability of red cells derived from gravity-separated placental blood with a hollow-fiber system","volume":"47","author":"T Brune","year":"2007","journal-title":"Transfusion"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"175234","DOI":"10.1155\/2009\/175234","article-title":"Quality, stability, and safety data of packed red cells and plasma processed by gravity separation using a new fully integrated hollow-fibre filter device","volume":"2009","author":"T Brune","year":"2009","journal-title":"Advances in hematology"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.1423-0410.2005.00660.x","article-title":"Separation of whole blood into plasma and red cells by using a hollow-fibre filtration system","volume":"89","author":"V Hornsey","year":"2005","journal-title":"Vox sanguinis"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"205","DOI":"10.7326\/M14-1589","article-title":"Platelet transfusion: a Clinical Practice Guideline From the AABBPlatelet transfusion: a clinical practice guideline from the AABB","volume":"162","author":"RM Kaufman","year":"2015","journal-title":"Annals of internal medicine"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"064113","DOI":"10.1063\/1.4936371","article-title":"Isolation and enrichment of low abundant particles with insulator-based dielectrophoresis","volume":"9","author":"A LaLonde","year":"2015","journal-title":"Biomicrofluidics"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"34122","DOI":"10.1063\/1.3640045","article-title":"Separation of platelets from other blood cells in continuous-flow by dielectrophoresis field-flow-fractionation","volume":"5","author":"N Piacentini","year":"2011","journal-title":"Biomicrofluidics"},{"issue":"10","key":"ref26","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1002\/pd.2079","article-title":"A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women","volume":"28","author":"R Huang","year":"2008","journal-title":"Prenatal diagnosis"},{"issue":"12","key":"ref27","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1039\/b809830c","article-title":"Egg beater as centrifuge: isolating human blood plasma from whole blood in resource-poor settings","volume":"8","author":"AP Wong","year":"2008","journal-title":"Lab on a chip"},{"issue":"23","key":"ref28","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.1039\/C4LC00785A","article-title":"Controlled incremental filtration: a simplified approach to design and fabrication of high-throughput microfluidic devices for selective enrichment of particles","volume":"14","author":"SC Gifford","year":"2014","journal-title":"Lab on a chip"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"35943","DOI":"10.1038\/srep35943","article-title":"A high-throughput microfluidic approach for 1000-fold leukocyte reduction of platelet-rich plasma","volume":"6","author":"H Xia","year":"2016","journal-title":"Scientific reports"},{"issue":"4","key":"ref30","doi-asserted-by":"crossref","first-page":"046503","DOI":"10.1063\/1.3659016","article-title":"Sealing SU-8 microfluidic channels using PDMS","volume":"5","author":"Z Zhang","year":"2011","journal-title":"Biomicrofluidics"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.colsurfb.2009.08.036","article-title":"The mechanism of erythrocyte sedimentation. Part 1: Channeling in sedimenting blood","volume":"75","author":"A Pribush","year":"2010","journal-title":"Colloids Surf B Biointerfaces"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.colsurfb.2009.08.037","article-title":"The mechanism of erythrocyte sedimentation. Part 2: The global collapse of settling erythrocyte network","volume":"75","author":"A Pribush","year":"2010","journal-title":"Colloids Surf B Biointerfaces"},{"key":"ref33","article-title":"Mosby\u2019s Manual of Diagnostic and Laboratory Tests-E-Book","author":"KD Pagana","year":"2013"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.dss.2010.06.005","article-title":"A DSS to manage platelet production supply chain for regional blood centers","volume":"50","author":"P Ghandforoush","year":"2010","journal-title":"Decision Support Systems"},{"issue":"6","key":"ref35","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1111\/trf.13511","article-title":"Storage of platelets at 4 degrees C in platelet additive solutions prevents aggregate formation and preserves platelet functional responses","volume":"56","author":"TM Getz","year":"2016","journal-title":"Transfusion"},{"issue":"5","key":"ref36","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/tme.12325","article-title":"PAS or plasma for storage of platelets? A concise review","volume":"26","author":"PF van der Meer","year":"2016","journal-title":"Transfus Med"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1046\/j.1365-3148.2001.00280.x","article-title":"Platelet washing to prevent recurrent febrile reactions to leucocyte-reduced transfusions","volume":"11","author":"TD Vo","year":"2001","journal-title":"Transfus Med"},{"issue":"3","key":"ref38","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/PCC.0b013e31822f173c","article-title":"Washing red blood cells and platelets transfused in cardiac surgery reduces postoperative inflammation and number of transfusions: results of a prospective, randomized, controlled clinical trial","volume":"13","author":"JM Cholette","year":"2012","journal-title":"Pediatr Crit Care Med"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1053\/j.semperi.2012.04.005","article-title":"Effect of processing and storage on red blood cell function in vivo","volume":"36","author":"A Doctor","year":"2012","journal-title":"Seminars in perinatology"},{"key":"ref40","article-title":"A Cellular Model of Shear-Induced Hemolysis","author":"S Sohrabi","year":"2017","journal-title":"Artificial organs"},{"issue":"3","key":"ref41","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1213\/ane.0b013e3181930a6e","article-title":"Noninfectious serious hazards of transfusion","volume":"108","author":"JE Hendrickson","year":"2009","journal-title":"Anesthesia and analgesia"},{"issue":"5","key":"ref42","doi-asserted-by":"crossref","first-page":"E124","DOI":"10.1038\/35074652","article-title":"Coping with the inevitable: how cells repair a torn surface membrane","volume":"3","author":"PL McNeil","year":"2001","journal-title":"Nature cell biology"},{"issue":"4","key":"ref43","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1161\/CIRCULATIONAHA.110.008698","article-title":"Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage lesion","volume":"124","author":"C Donadee","year":"2011","journal-title":"Circulation"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1111\/j.1537-2995.2011.03100.x","article-title":"Storage lesion: role of red blood cell breakdown","volume":"51","author":"DB Kim-Shapiro","year":"2011","journal-title":"Transfusion"},{"issue":"8","key":"ref45","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1111\/trf.13052","article-title":"Washing stored red blood cells in an albumin solution improves their morphologic and hemorheologic properties","volume":"55","author":"WH Reinhart","year":"2015","journal-title":"Transfusion"},{"issue":"21","key":"ref46","doi-asserted-by":"crossref","first-page":"4284","DOI":"10.1182\/blood-2009-10-245001","article-title":"Transfusion of red blood cells after prolonged storage produces harmful effects that are mediated by iron and inflammation","volume":"115","author":"EA Hod","year":"2010","journal-title":"Blood"},{"issue":"1","key":"ref47","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1097\/MAT.0b013e31815d6898","article-title":"Platelet activation due to hemodynamic shear stresses: damage accumulation model and comparison to in vitro measurements","volume":"54","author":"M Nobili","year":"2008","journal-title":"ASAIO journal (American Society for Artificial Internal Organs: 1992)"},{"issue":"5","key":"ref48","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1055\/s-0037-1613308","article-title":"Platelet aggregation and activation under complex patterns of shear stress","volume":"88","author":"J-n Zhang","year":"2002","journal-title":"Thrombosis and haemostasis"},{"issue":"11","key":"ref49","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1111\/j.1537-2995.2008.01836.x","article-title":"Implementation of buffy coat platelet component production: comparison to platelet-rich plasma platelet production","volume":"48","author":"E Levin","year":"2008","journal-title":"Transfusion"},{"issue":"1","key":"ref50","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1046\/j.1365-2141.1997.1572983.x","article-title":"Activation during preparation of therapeutic platelets affects deterioration during storage: a comparative flow cytometric study of different production methods","volume":"98","author":"P Metcalfe","year":"1997","journal-title":"British journal of haematology"},{"issue":"1","key":"ref51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1423-0410.2009.01295.x","article-title":"Buffy-coat-derived pooled platelet concentrates and apheresis platelet concentrates: which product type should be preferred?","volume":"99","author":"H Schrezenmeier","year":"2010","journal-title":"Vox sanguinis"},{"issue":"2","key":"ref52","first-page":"129","article-title":"Platelet concentrates, from whole blood or collected by apheresis?","volume":"48","author":"PF van der Meer","year":"2013","journal-title":"Transfusion and apheresis science: official journal of the World Apheresis Association: official journal of the European Society for Haemapheresis"},{"issue":"7","key":"ref53","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1111\/j.1537-2995.2008.01733.x","article-title":"Platelet products prepared by different methods of sedimentation undergo platelet activation differently during storage","volume":"48","author":"A Skripchenko","year":"2008","journal-title":"Transfusion"},{"issue":"10","key":"ref54","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1111\/j.1537-2995.2004.04042.x","article-title":"Role of platelet surface glycoprotein Ibalpha and P-selectin in the clearance of transfused platelet concentrates","volume":"44","author":"V Leytin","year":"2004","journal-title":"Transfusion"},{"issue":"4","key":"ref55","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.tmrv.2007.05.004","article-title":"Hemostatic and signaling functions of transfused platelets","volume":"21","author":"S Cauwenberghs","year":"2007","journal-title":"Transfus Med Rev"},{"issue":"4","key":"ref56","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3109\/10409238.2015.1050550","article-title":"How it all starts: initiation of the clotting cascade","volume":"50","author":"SA Smith","year":"2015","journal-title":"Critical reviews in biochemistry and molecular biology"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"133","DOI":"10.2147\/VHRM.S44469","article-title":"Platelet function tests: a comparative review","volume":"11","author":"R Paniccia","year":"2015","journal-title":"Vascular health and risk management"},{"issue":"2","key":"ref58","first-page":"214","article-title":"Leucoreduction of blood components: an effective way to increase blood safety?","volume":"14","author":"M Bianchi","year":"2016","journal-title":"Blood Transfus"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0190827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T09:37:18Z","timestamp":1589017038000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0190827"}},"subtitle":[],"editor":[{"given":"Halvard","family":"B\u00f6nig","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,1,18]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,1,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0190827","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,18]]}}}