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Bertollo","year":"2015"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.ympev.2005.09.018","article-title":"Molecular phylogeny of the Southeast Asian freshwater fish family Botiidae (Teleostei: Cobitoidea) and the origin of polyploidy in their evolution","volume":"39","author":"V \u0160lechtov\u00e1","year":"2006","journal-title":"Mol Phylogenet Evol"},{"key":"ref45","first-page":"1255","article-title":"Universal PCR primers for S7 ribosomal protein gene introns in fish","volume":"7","author":"S Chow","year":"1998","journal-title":"Mol Ecol"},{"key":"ref46","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"T. A Hall","year":"1999","journal-title":"Nucleic Acids Symp Ser"},{"key":"ref47","author":"D Swofford","year":"2000"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","article-title":"MRBAYES: Bayesian inference of phylogenetic trees","volume":"17","author":"JP Huelsenbeck","year":"2001","journal-title":"Bioinformatics"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.aquaculture.2010.02.003","article-title":"Many branches, one root: First evidence for a monophyly of the morphologically highly diverse goldfish (<italic>Carassius auratus<\/italic>)","volume":"302","author":"K Rylkov\u00e1","year":"2010","journal-title":"Aquaculture"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/j.1095-8649.2006.01290.x","article-title":"First European record of <italic>Carassius langsdorfii<\/italic> from the Elbe basin","volume":"70","author":"L Kalous","year":"2007","journal-title":"J Fish Biol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1111\/j.1095-8649.2012.03492.x","article-title":"New mtDNA data reveal a wide distribution of the Japanese ginbuna <italic>Carassius langsdorfii<\/italic> in Europe","volume":"82","author":"L Kalous","year":"2013","journal-title":"J Fish Biol"},{"key":"ref52","first-page":"11","article-title":"Hidden diversity within the Prussian carp and designation of a neotype for <italic>Carassius gibelio<\/italic> (Teleostei: Cyprinidae)","volume":"23","author":"L Kalous","year":"2012","journal-title":"Ichthyol Explor Freshwaters"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1093\/oxfordjournals.jbchem.a132601","article-title":"Nucleotide sequence of 5S ribosomal RNA from rainbow trout (<italic>Salmo gairdnerii<\/italic>) liver","volume":"86","author":"H Komiya","year":"1979","journal-title":"J 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rDNA chromosomal localisation revealed by FISH in <italic>Astyanax scabripinnis<\/italic> (Pisces, Characidae)","volume":"123","author":"M Mantovani","year":"2005","journal-title":"Genetica"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1159\/000097429","article-title":"Karyotype differentiation in <italic>Chromaphyosemion<\/italic> killifishes (Cyprinodontiformes, Nothobranchiidae). III: extensive karyotypic variability associated with low mitochondrial haplotype differentiation in <italic>C<\/italic>. <italic>bivittatum<\/italic>","volume":"116","author":"M Volker","year":"2007","journal-title":"Cytogenet Genome Res"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1159\/000084211","article-title":"Lack of correspondence between CMA<sub>3<\/sub>-, Ag-positive signals and 28S rDNA loci in two Iberian minnows (Teleostei, Cyprinidae) evidenced by sequential banding","volume":"109","author":"M Gromicho","year":"2005","journal-title":"Cytogenet Genome Res"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1159\/000131803","article-title":"A comparison of the karyotype, constitutive heterochromatin, and nucleolar organizer regions of the new tetraploid species <italic>Xenopus epitropicalis<\/italic> Fischberg and Picard with those of <italic>Xenopus tropicalis<\/italic> Gray (Anura, Pipidae)","volume":"34","author":"J Tymowska","year":"1982","journal-title":"Cytogenet Cell Genet"},{"key":"ref77","doi-asserted-by":"crossref","first-page":"e0177087","DOI":"10.1371\/journal.pone.0177087","article-title":"Chromosome divergence during evolution of the tetraploid clawed frogs, <italic>Xenopus mellotropicalis<\/italic> and <italic>Xenopus epitropicalis<\/italic> as revealed by Zoo-FISH","volume":"12","author":"M Knytl","year":"2017","journal-title":"PLoS One"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1007\/BF01938973","article-title":"A chromosome mutation affecting the number of nucleoli in <italic>Xenopus borealis<\/italic> Parker","volume":"30","author":"M Jotterand","year":"1974","journal-title":"Experientia"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1159\/000353464","article-title":"Rise and persistence of animal polyploidy: evolutionary constraints and potential","volume":"140","author":"L Choleva","year":"2013","journal-title":"Cytogenet Genome Res"},{"key":"ref80","first-page":"215","article-title":"Red List of the ichthyofauna of the Czech Republic: development and present status","volume":"53","author":"S Lusk","year":"2004","journal-title":"Folia Zool"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0006-3207(94)00083-3","article-title":"Endangered freshwater fishes of Greece","volume":"72","author":"PS Economidis","year":"1995","journal-title":"Biol Conserv"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0190924","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:47:42Z","timestamp":1540342062000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0190924"}},"subtitle":[],"editor":[{"given":"Zuogang","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,1,23]]},"references-count":81,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,1,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0190924","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,23]]}}}