{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T01:22:19Z","timestamp":1784769739309,"version":"3.55.0"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T00:00:00Z","timestamp":1517961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0191596","type":"journal-article","created":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T18:32:36Z","timestamp":1518028356000},"page":"e0191596","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Molecular characterization of the recombinant protein RmLTI-BmCG-LTB: Protective immunity against Rhipicephalus (Boophilus) microplus"],"prefix":"10.1371","volume":"13","author":[{"given":"B\u00e1rbara Guimar\u00e3es","family":"Csordas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Casquero","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Val\u00e9rio","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Silva","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Leivas","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0739-2997","authenticated-orcid":true,"given":"Renato","family":"Andreotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,2,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"4728","DOI":"10.1016\/j.vaccine.2013.08.031","article-title":"Lesser protein degradation machinery correlates with higher BM86 tick vaccine efficacy in <italic>Rhipicephalus annulatus<\/italic> when compared to <italic>Rhipicephalus microplus<\/italic>","volume":"31","author":"M Popara","year":"2013","journal-title":"Vaccine"},{"key":"ref2","first-page":"97","article-title":"Performance of two Bm86 antigen vaccin formulation against tick using crossbreed bovines in stall test","volume":"15","author":"R Andreotti","year":"2006","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1186\/s13071-017-2136-2","article-title":"Mining a differential sialotranscriptome of <italic>Rhipicephalus microplus<\/italic> guides antigen discovery to formulate a vaccine that reduces tick infestations","volume":"10","author":"SR Maruyama","year":"2017","journal-title":"Parasit Vectors"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1590\/S1984-29612008000200006","article-title":"Comparison of predicted binders in <italic>Rhipicephalus<\/italic> (<italic>Boophilus<\/italic>) <italic>microplus<\/italic> intestine protein variants bm86 campo grande strain, Bm86 and Bm95","volume":"17","author":"R Andreotti","year":"2008","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1590\/S1984-29612011000200003","article-title":"<italic>Rhipicephalus<\/italic> (<italic>Boophilus<\/italic>) <italic>microplus<\/italic>: expression and characterization of Bm86-CG in Pichia pastoris","volume":"20","author":"RC Cunha","year":"2011","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1590\/S1984-29612012000300014","article-title":"Bovine immunoprotection against <italic>Rhipicephalus<\/italic> (<italic>Boophilus<\/italic>) <italic>microplus<\/italic> with recombinant Bm86-Campo Grande antigen","volume":"21","author":"RC Cunha","year":"2012","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref7","first-page":"20","article-title":"Serine Proteinase Inhibitors from Eggs and Larvae of Tick <italic>Boophilus microplus<\/italic>: Purification and Biochemical Characterization","author":"R Andreotti","year":"2001","journal-title":"J. Protein Chem"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S1567-5769(01)00203-X","article-title":"BmTI antigens induce a bovine protective immune response against <italic>Boophilus microplus<\/italic> tick","volume":"2","author":"R Andreotti","year":"2002","journal-title":"Int Immunopharmacol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"6678","DOI":"10.1016\/j.vaccine.2012.08.066","article-title":"Protective immunity against tick infestation in cattle vaccinated with recombinant trypsin inhibitor of <italic>Rhipicephalus microplus<\/italic>","volume":"30","author":"R Andreotti","year":"2012","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/0264-410X(94)90118-X","article-title":"Synergistic action of cholera toxin B subunit (and <italic>Escherichia coli<\/italic> heat-labile toxin B subunit) and a trace amount of cholera whole toxin as an adjuvant for nasal influenza vaccine","volume":"12","author":"S Tamura","year":"1994","journal-title":"Vaccine"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"5734","DOI":"10.1016\/j.vaccine.2006.04.036","article-title":"A recombinant chimera composed of R1 repeat region of <italic>Mycoplasma hyopneumoniae<\/italic> P97 adhesin with <italic>Escherichia coli<\/italic> heat-labile enterotoxin B subunit elicits immune response in mice","volume":"24","author":"FR Concei\u00e7\u00e3o","year":"2006","journal-title":"Vaccine"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1128\/CVI.05720-11","article-title":"Protection against lethal leptospirosis after vaccination with LipL32 coupled or coadministered with the b subunit of <italic>Escherichia coli<\/italic> heat-labile enterotoxin","volume":"19","author":"AA Grassmann","year":"2012","journal-title":"Clin Vaccine Immunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1128\/CVI.00016-14","article-title":"<italic>Salmonella enterica<\/italic> serovar enteritidis ghosts carrying the <italic>Escherichia coli<\/italic> heat-labile enterotoxin B subunit are capable of inducing enhanced protective immune responses","volume":"21","author":"C V Jawale","year":"2014","journal-title":"Clin Vaccine Immunol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/JVI.55.3.836-839.1985","article-title":"Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide","volume":"55","author":"EA Emini","year":"1985","journal-title":"J Virol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1385\/1-59259-890-0:571","article-title":"Protein Identification and Analysis Tools on the ExPASy Server","author":"E Gasteiger","year":"2005","journal-title":"Proteomics Protoc Handb"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/nar\/gku340","article-title":"SWISS-MODEL: Modelling protein tertiary and quaternary structure using evolutionary information","volume":"42","author":"M Biasini","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1139\/W08-056","article-title":"B subunit of <italic>Escherichia coli<\/italic> heat-labile enterotoxin as adjuvant of humoral immune response in recombinant BCG vaccination","volume":"54","author":"SR Rocha A da","year":"2008","journal-title":"Can J Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0378-1119(79)90082-9","article-title":"Prolonged incubation in calcium chloride improves the competence of <italic>Escherichia coli<\/italic> cells","volume":"6","author":"M Dagert","year":"1979","journal-title":"Gene"},{"key":"ref19","first-page":"459","article-title":"Vaccine Design","volume":"1404","author":"B Kumar","year":"2016","journal-title":"Vaccine Design"},{"key":"ref20","article-title":"Molecular Cloning: A Laboratory Manual","author":"J Sambrook","year":"2001"},{"key":"ref21","article-title":"Methods Enzymol","author":"JC Lessard","year":"2013"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/1746-6148-6-43","article-title":"Two initial vaccinations with the Bm86-based Gavacplus vaccine against <italic>Rhipicephalus<\/italic> (<italic>Boophilus<\/italic>) <italic>microplus<\/italic> induce similar reproductive suppression to three initial vaccinations under production conditions","volume":"6","author":"M Vargas","year":"2010","journal-title":"BMC Vet Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1023\/A:1006270615158","article-title":"Sequence variations in the <italic>Boophilus microplus<\/italic> Bm86 locus and implications for immunoprotection in cattle vaccinated with this antigen","volume":"23","author":"JC Garc\u00eda-Garc\u00eda","year":"1999","journal-title":"Exp Appl Acarol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"5734","DOI":"10.1016\/j.vaccine.2006.04.036","article-title":"A recombinant chimera composed of R1 repeat region of <italic>Mycoplasma hyopneumoniae<\/italic> P97 adhesin with <italic>Escherichia coli<\/italic> heat-labile enterotoxin B subunit elicits immune response in mice","volume":"24","author":"FR Concei\u00e7\u00e3o","year":"2006","journal-title":"Vaccine"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1083\/jcb.67.3.852","article-title":"Transfer of proteins across membranes. II. Reconstitution of functional rough microscomes from heterologous components","volume":"67","author":"G Blobel","year":"1975","journal-title":"J Cell Biol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1745-7580-2-2","article-title":"Improved method for predicting linear B-cell epitopes","volume":"2","author":"JEP Larsen","year":"2006","journal-title":"Immunome Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.vetpar.2016.02.032","article-title":"Design of the ATAQ peptide and its evaluation as an immunogen to develop a <italic>Rhipicephalus<\/italic> vaccine","volume":"221","author":"AR Aguirre Ade","year":"2016","journal-title":"Vet Parasitol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.ttbdis.2012.01.003","article-title":"A systematic, functional genomics, and reverse vaccinology approach to the identification of vaccine candidates in the cattle tick, <italic>Rhipicephalus microplus<\/italic>","volume":"3","author":"C Maritz-Olivier","year":"2012","journal-title":"Ticks Tick Borne Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"7044","DOI":"10.1128\/JB.185.24.7044-7052.2003","article-title":"The Growth Advantage in Stationary-Phase Phenotype Conferred by rpoS Mutations Is Dependent on the pH and Nutrient Environment","volume":"185","author":"MJ Farrell","year":"2003","journal-title":"J Bacteriol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1128\/JB.187.1.304-319.2005","article-title":"pH Regulates Genes for Flagellar Motility, Catabolism, and Oxidative Stress in <italic>Escherichia coli<\/italic> K-12 pH Regulates Genes for Flagellar Motility, Catabolism, and Oxidative Stress in <italic>Escherichia coli<\/italic> K-12 \u2020","volume":"187","author":"LM Maurer","year":"2005","journal-title":"J Bacteriol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"4442","DOI":"10.1128\/AEM.00722-15","article-title":"Rich medium composition affects <italic>Escherichia coli<\/italic> survival, glycation, and mutation frequency during long-term batch culture","volume":"81","author":"KE Kram","year":"2015","journal-title":"Appl Environ Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.bej.2010.01.014","article-title":"Effect of mechanical agitation on the production of cellulases by Trichoderma reesei RUT-C30 in a draft-tube airlift bioreactor","volume":"49","author":"A Ahamed","year":"2010","journal-title":"Biochem Eng J"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1007\/s12010-011-9261-8","article-title":"Efficient production of a thermophilic 2-Deoxyribose-5-Phosphate aldolase in glucose-limited fed-batch cultivations of <italic>Escherichia coli<\/italic> by continuous lactose induction strategy","volume":"165","author":"XL Pei","year":"2011","journal-title":"Appl Biochem Biotechnol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/j.enzmictec.2007.07.006","article-title":"Expression kinetics and plasmid stability of recombinant <italic>E<\/italic>. <italic>coli<\/italic> encoding urease-derived peptide with bioinsecticide activity","volume":"41","author":"G Tomazetto","year":"2007","journal-title":"Enzyme Microb Technol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/1475-2859-9-18","article-title":"Production of recombinant proteins in <italic>E<\/italic>. <italic>coli<\/italic> by the heat inducible expression system based on the phage lambda pL and\/or pR promoters","volume":"9","author":"N a Valdez-Cruz","year":"2010","journal-title":"Microb Cell Fact"},{"key":"ref36","first-page":"1","article-title":"Production and Evaluation of a Recombinant Chimeric Vaccine against <italic>Clostridium botulinum<\/italic> Neurotoxin Types C and D","volume":"8","author":"LAF Gil","year":"2013","journal-title":"PLoS One"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s10493-017-0156-4","article-title":"Evaluation of three adjuvants with respect to both adverse effects and the efficacy of antibody production to the Bm86 protein","volume":"72","author":"J Petermann","year":"2017","journal-title":"Exp Appl Acarol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.rvsc.2010.05.037","article-title":"Recombinant mid gut antigen (Bm95) as a vaccine against Indian <italic>Rhiphicephalus haemaphysaloides<\/italic> in <italic>Bos indicus<\/italic> cattle","volume":"90","author":"P Sugumar","year":"2011","journal-title":"Res Vet Sci. Elsevier Ltd"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0191596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T21:30:11Z","timestamp":1603834211000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0191596"}},"subtitle":[],"editor":[{"given":"Paulo Lee","family":"Ho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,2,7]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,2,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0191596","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,7]]}}}