{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:07:39Z","timestamp":1761156459240,"version":"3.37.3"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T00:00:00Z","timestamp":1519171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Tokyo, Japan","award":["No. 25462585"],"award-info":[{"award-number":["No. 25462585"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0193061","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T16:00:49Z","timestamp":1519401649000},"page":"e0193061","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Separate analysis of human papillomavirus E6 and E7 messenger RNAs to predict cervical neoplasia progression"],"prefix":"10.1371","volume":"13","author":[{"given":"Shuling","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3577-5124","authenticated-orcid":true,"given":"Takeo","family":"Minaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bouchra","family":"Lachkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Tenjimbayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayumi","family":"Shikama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobutaka","family":"Tasaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azusa","family":"Akiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Sakurai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sari","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ochi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamiko","family":"Onuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyomi","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,2,21]]},"reference":[{"key":"ref1","article-title":"Cancer fact sheets: Cervical cancer","author":"M Ervik","year":"2016","journal-title":"Cancer Today"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/nrc3728","article-title":"Clinical implications of (epi)genetic changes in HPV-induced cervical precancerous lesions","volume":"14","author":"RD Steenbergen","year":"2014","journal-title":"Nat Rev Cancer"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1128\/JVI.77.2.1551-1563.2003","article-title":"Endogenous Human Papillomavirus E6 and E7 Proteins Differentially Regulate Proliferation, Senescence, and Apoptosis in HeLa Cervical Carcinoma Cells","volume":"77","author":"RA DeFilippis","year":"2003","journal-title":"Journal of Virology"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/rmv.605","article-title":"Molecular mechanisms of cervical carcinogenesis by high-risk human papillomaviruses: novel functions of E6 and E7 oncoproteins","volume":"19","author":"T Yugawa","year":"2009","journal-title":"Rev Med Virol"},{"issue":"9","key":"ref5","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1128\/JVI.80.9.4249-4263.2006","article-title":"The E7 oncoprotein is translated from spliced E6*I transcripts in high-risk human papillomavirus type 16- or type 18-positive cervical cancer cell lines via translation reinitiation","volume":"80","author":"S Tang","year":"2006","journal-title":"J Virol"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/1096-9071(200010)62:2<251::AID-JMV18>3.0.CO;2-V","article-title":"Ubiquitous presence of E6 and E7 transcripts in human papillomavirus-positive cervical carcinomas regardless of its type","volume":"62","author":"S Nakagawa","year":"2000","journal-title":"J Med Virol"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1002\/jmv.20153","article-title":"Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reaction","volume":"74","author":"K Sotlar","year":"2004","journal-title":"J Med Virol"},{"key":"ref8","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4612-2042-8","article-title":"The Bethesda System for Reporting Cervical Cytology","author":"D Solomon","year":"2004"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/j.1447-0756.2012.01858.x","article-title":"Guidelines for office gynecology in Japan: Japan Society of Obstetrics and Gynecology and Japan Association of Obstetricians and Gynecologists 2011 edition","volume":"38","author":"T Takeda","year":"2012","journal-title":"J Obstet Gynaecol Res"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1002\/jmv.23487","article-title":"Comparison of oncogenic HPV type-specific viral DNA load and E6\/E7 mRNA detection in cervical samples: results from a multicenter study","volume":"85","author":"F Broccolo","year":"2013","journal-title":"J Med Virol"},{"issue":"12","key":"ref11","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1002\/j.1460-2075.1989.tb08570.x","article-title":"HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes","volume":"8","author":"P Hawley-Nelson","year":"1989","journal-title":"EMBO J"},{"issue":"10","key":"ref12","doi-asserted-by":"crossref","first-page":"4417","DOI":"10.1128\/JVI.63.10.4417-4421.1989","article-title":"The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes","volume":"63","author":"K Munger","year":"1989","journal-title":"J Virol"},{"issue":"16","key":"ref13","first-page":"4862","article-title":"Dissection of human papillomavirus E6 and E7 function in transgenic mouse models of cervical carcinogenesis","volume":"63","author":"RR Riley","year":"2003","journal-title":"Cancer Res"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1097\/00004347-200404000-00003","article-title":"Quantitative molecular parameters to identify low-risk and high-risk early CIN lesions: role of markers of proliferative activity and differentiation and Rb availability","volume":"23","author":"AJ Kruse","year":"2004","journal-title":"Int J Gynecol Pathol"},{"issue":"8","key":"ref15","doi-asserted-by":"crossref","first-page":"2779","DOI":"10.1128\/JCM.00382-10","article-title":"Clinical performance of the PreTect HPV-Proofer E6\/E7 mRNA assay in comparison with that of the Hybrid Capture 2 test for identification of women at risk of cervical cancer","volume":"48","author":"S Ratnam","year":"2010","journal-title":"J Clin Microbiol"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"144 e1","DOI":"10.1016\/j.ajog.2012.12.003","article-title":"APTIMA HPV assay performance in women with atypical squamous cells of undetermined significance cytology results","volume":"208","author":"MH Stoler","year":"2013","journal-title":"Am J Obstet Gynecol"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.ygyno.2010.09.013","article-title":"Quantification of intracellular HPV E6\/E7 mRNA expression increases the specificity and positive predictive value of cervical cancer screening compared to HPV DNA","volume":"120","author":"G Coquillard","year":"2011","journal-title":"Gynecol Oncol"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1128\/JCM.02147-10","article-title":"Aptima HPV E6\/E7 mRNA test is as sensitive as Hybrid Capture 2 Assay but more specific at detecting cervical precancer and cancer","volume":"49","author":"S Ratnam","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"10","key":"ref19","doi-asserted-by":"crossref","first-page":"687","DOI":"10.7326\/0003-4819-155-10-201111150-00376","article-title":"Liquid-based cytology and human papillomavirus testing to screen for cervical cancer: a systematic review for the U.S. Preventive Services Task Force","volume":"155","author":"EP Whitlock","year":"2011","journal-title":"Ann Intern Med"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"e90023","DOI":"10.1371\/journal.pone.0090023","article-title":"Triage of HR-HPV positive women with minor cytological abnormalities: a comparison of mRNA testing, HPV DNA testing, and repeat cytology using a 4-year follow-up of a population-based study","volume":"9","author":"M Persson","year":"2014","journal-title":"PLoS One"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"e0124460","DOI":"10.1371\/journal.pone.0124460","article-title":"Presence of High-Risk HPV mRNA in Relation to Future High-Grade Lesions among High-Risk HPV DNA Positive Women with Minor Cytological Abnormalities","volume":"10","author":"H Johansson","year":"2015","journal-title":"PLoS One"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1002\/cncy.21233","article-title":"Evaluation of p16(INK4a)\/Ki-67 dual stain in comparison with an mRNA human papillomavirus test on liquid-based cytology samples with low-grade squamous intraepithelial lesion","volume":"121","author":"M Waldstrom","year":"2013","journal-title":"Cancer Cytopathol"},{"issue":"6","key":"ref23","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/j.1365-2303.2011.00923.x","article-title":"Comparison of the clinical performance of an HPV mRNA test and an HPV DNA test in triage of atypical squamous cells of undetermined significance (ASC-US)","volume":"23","author":"M Waldstrom","year":"2012","journal-title":"Cytopathology"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1136\/jclinpath-2013-202014","article-title":"Evaluation of commercial HPV assays in the context of post-treatment follow-up: Scottish Test of Cure Study (STOCS-H)","volume":"67","author":"HA Cubie","year":"2014","journal-title":"J Clin Pathol"},{"issue":"7","key":"ref25","doi-asserted-by":"crossref","DOI":"10.3390\/ijms18071480","article-title":"Analytic and Diagnostic Performances of Human Papillomavirus E6\/E7 mRNA Test on up-to 11-Year-Old Liquid-Based Cervical Samples. A Biobank-Based Longitudinal Study","volume":"18","author":"R Zappacosta","year":"2017","journal-title":"Int J Mol Sci"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"e57600","DOI":"10.1371\/journal.pone.0057600","article-title":"Prognostic value of HPV E6\/E7 mRNA assay in women with negative colposcopy or CIN1 histology result: a follow-up study","volume":"8","author":"P Giorgi Rossi","year":"2013","journal-title":"PLoS One"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1309\/AJCPHVD7MIP3FYVV","article-title":"Human papillomavirus oncogenic mRNA testing for cervical cancer screening: baseline and longitudinal results from the CLEAR study","volume":"144","author":"JL Reid","year":"2015","journal-title":"Am J Clin Pathol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0193061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T16:30:53Z","timestamp":1603902653000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0193061"}},"subtitle":[],"editor":[{"given":"Maria Lina","family":"Tornesello","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,2,21]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,2,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0193061","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2018,2,21]]}}}