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Septic keratitis in dogs, cats and horses in Switzerland; causative bacteria and antibiotic susceptibility. In: Proceedings of the 47th Annual Conference of the American College of Veterinary Ophthalmologists; 2016; Monterey, California, USA. p.32."},{"key":"ref5","unstructured":"Martinez JA, da Costa PD. Evaluation of bacterial isolates and antibiotic susceptibility in cats and dogs presented with ulcerative keratitis to a referring practice in New Orleans, LA between 2011 and 2015. 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Super-oxidized water (Microcyn 60) for mesh hernioplasty in grossly contaminated fields: an experimental study. In: Proceedings of American College of Surgeons 91st Annual Clinical Congress; 2005; San Francisco, California, USA. p.190."},{"key":"ref46","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1177\/1040638714551403","article-title":"<italic>Moraxella spp<\/italic>. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013","volume":"26","author":"JD Loy","year":"2014","journal-title":"J Vet Diagn Invest"},{"key":"ref47","article-title":"CLSI document M07-A10","year":"2015"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"e101037","DOI":"10.1371\/journal.pone.0101037","article-title":"In vitro bactericidal and bacteriolytic activity of ceragenin CSA-13 against planktonic cultures and biofilms of Streptococcus pneumoniae and other pathogenic streptococci","volume":"9","author":"M Moscoso","year":"2014","journal-title":"PLoS One"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3109\/02713689009044524","article-title":"Corneal epithelial wound healing: a tissue culture assay on the effect of antibiotics","volume":"9","author":"JD Nelson","year":"1990","journal-title":"Curr Eye Res"},{"key":"ref50","first-page":"656","article-title":"In vitro effects of aminoglycosides and fluoroquinolones on keratocytes","volume":"37","author":"B Seitz","year":"1996","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"4","key":"ref51","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/j.1834-7819.2001.tb00291.x","article-title":"The shelf-life of sodium hypochlorite irrigating solutions","volume":"46","author":"RM Clarkson","year":"2001","journal-title":"Aust Dent J"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0193217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T02:27:37Z","timestamp":1603938457000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0193217"}},"subtitle":[],"editor":[{"given":"Kalimuthusamy","family":"Natarajaseenivasan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,3,7]]},"references-count":51,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,3,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0193217","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,7]]}}}