{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:18:25Z","timestamp":1789993105793,"version":"4.0.1"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture, Food and Drug Safety and Kangwon National University","award":["14162MFDS085"],"award-info":[{"award-number":["14162MFDS085"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0193277","type":"journal-article","created":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T13:40:15Z","timestamp":1519825215000},"page":"e0193277","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["In vivo screening platform for shiga toxin-producing Escherichia coli (STEC) using Caenorhabditis elegans as a model"],"prefix":"10.1371","volume":"13","author":[{"given":"Su-Bin","family":"Hwang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9421-0102","authenticated-orcid":true,"given":"Jung-gu","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung-Jae","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramachandran","family":"Chelliah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7472-0436","authenticated-orcid":true,"given":"Deog-Hwan","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,2,28]]},"reference":[{"key":"ref1","article-title":"Characterization of enteropathogenic and Shiga toxin-producing Escherichia coli in cattle and deer in a shared agroecosystem","volume":"5","author":"P Singh","year":"2015"},{"key":"ref2","first-page":"109","article-title":"<italic>Applied Genomics of Foodborne Pathogens<\/italic>","author":"V Michelacci","year":"2017"},{"issue":"1","key":"ref3","first-page":"44","article-title":"Strain-specific impact of the high-pathogenicity island on virulence in extra-intestinal pathogenic Escherichia coli","volume":"307","author":"M Smati","year":"2017"},{"issue":"1","key":"ref4","first-page":"82","article-title":"Enterohaemorrhagic Escherichia coli O157: H7 Shiga like toxin 1 is required for full pathogenicity and activation of the p38 mitogen activated protein kinase pathway in Caenorhabditis elegans","volume":"15","author":"TC Chou","year":"2013"},{"issue":"7","key":"ref5","first-page":"2435","article-title":"Evaluating the pathogenic potential of environmental Escherichia coli by using the Caenorhabditis elegans infection model","volume":"79","author":"A Merkx-Jacques","year":"2013"},{"issue":"1","key":"ref6","first-page":"e54456","article-title":"A family of indoles regulate virulence and Shiga toxin production in pathogenic E. coli","volume":"8","author":"B Bommarius","year":"2013"},{"key":"ref7","first-page":"36","article-title":"Global profiling of metabolic response of Caenorhabditis elegans against Escherichia coli O157: H7","volume":"53","author":"EJ Yun","year":"2017"},{"issue":"1","key":"ref8","first-page":"533","article-title":"2011 German Escherichia coli O104: H4 outbreak: whole-genome phylogeny without alignment","volume":"4","author":"MK Cheung","year":"2011"},{"issue":"3","key":"ref9","first-page":"437","article-title":"A case of hemolytic uremic syndrome caused by Escherichia coli O104: H4","volume":"47","author":"WK Bae","year":"2006"},{"issue":"6","key":"ref10","first-page":"939","article-title":"Multistate Outbreak of Escherichia coli O145 Infections Associated with Romaine Lettuce Consumption, 2010\u2021 \u00a7","volume":"76","author":"EV Taylor","year":"2013"},{"issue":"26","key":"ref11","first-page":"17020","article-title":"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli","volume":"99","author":"RA Welch","year":"2002"},{"issue":"4","key":"ref12","first-page":"446","article-title":"Rapid and reliable discrimination between Shigella species and Escherichia coli using MALDI-TOF mass spectrometry","volume":"305","author":"A Paauw","year":"2015"},{"key":"ref13","first-page":"16027","article-title":"Global phylogeography and evolutionary history of Shigelladysenteriae type 1","volume":"1","author":"E Njamkepo","year":"2016"},{"issue":"3","key":"ref14","article-title":"Sequencing a piece of history: complete genome sequence of the original Escherichia coli strain","volume":"3","author":"KA Dunne","year":"2017"},{"issue":"9","key":"ref15","first-page":"5080","article-title":"Molecular evolutionary relationships of enteroinvasive Escherichia coli and Shigella spp","volume":"72","author":"R Lan","year":"2004"},{"issue":"1","key":"ref16","first-page":"31","article-title":"Escherichia coli O157: H7 infection in Dutch belted and New Zealand white rabbits","volume":"60","author":"A Panda","year":"2010"},{"issue":"1","key":"ref17","first-page":"86","article-title":"Enterohemorrhagic Escherichia coli induce attaching and effacing lesions and hemorrhagic colitis in human and bovine intestinal xenograft models","volume":"4","author":"L Golan","year":"2011"},{"key":"ref18","doi-asserted-by":"crossref","DOI":"10.1155\/2011\/258185","article-title":"Mouse models of Escherichia coli O157: H7 infection and shiga toxin injection","author":"KL Mohawk","year":"2011"},{"key":"ref19","first-page":"57","article-title":"Exploiting the explosion of information associated with whole genome sequencing to tackle Shiga toxin-producing Escherichia coli (STEC) in global food production systems","volume":"187","author":"E Franz","year":"2014"},{"key":"ref20","unstructured":"Sarabhai S (2014) Molecular mechanism of terminaliachebula RETZ mediated quenching of Pseudomonas aeruginosa PAO1 virulence and use of Caenorhabditis elegans as the non mammalian model host system"},{"issue":"6","key":"ref21","first-page":"1343","article-title":"The evolution of plasticity of dauer larva developmental arrest in the nematode Caenorhabditis elegans","volume":"5","author":"SA Diaz","year":"2015"},{"issue":"3","key":"ref22","first-page":"275","article-title":"Use of Caenorhabditis elegans as a Genetic Model for Bacterial Pathogenesis Studies","volume":"29","author":"EAE Abada","year":"2007"},{"issue":"7065","key":"ref23","first-page":"179","article-title":"Pathogenic bacteria induce aversive olfactory learning in Caenorhabditis elegans","volume":"438","author":"Y Zhang","year":"2005"},{"issue":"4","key":"ref24","first-page":"988","article-title":"Paralysis and killing of Caenorhabditis elegans by enteropathogenic Escherichia coli requires the bacterial tryptophanase gene","volume":"57","author":"A Anyanful","year":"2005"},{"issue":"2","key":"ref25","first-page":"181","article-title":"A single gene network accurately predicts phenotypic effects of gene perturbation in Caenorhabditis elegans","volume":"40","author":"I Lee","year":"2008"},{"issue":"5\u20136","key":"ref26","first-page":"719","article-title":"An extracellular protease from Brevibacilluslatero sporus G4 without parasporal crystals can serve as a pathogenic factor in infection of nematodes","volume":"156","author":"X Huang","year":"2005"},{"issue":"4","key":"ref27","first-page":"210","article-title":"Control of lifespan by food bacteria, nutrient limitation and pathogenicity of food in C. elegans","volume":"132","author":"S So","year":"2011"},{"issue":"9","key":"ref28","first-page":"e106085","article-title":"Shigellaflexneri Infection in Caenorhabditis elegans: cytopathological examination and identification of host responses","volume":"9","author":"DT George","year":"2014"},{"issue":"3","key":"ref29","first-page":"1","article-title":"Virulence variations in Shigella and enteroinvasive Escherichia coli using the Caenorhabditis elegans model","volume":"362","author":"CC Fung","year":"2015"},{"key":"ref30","first-page":"97","article-title":"<italic>Escherichia coli in the Americas<\/italic>","author":"M Rivas","year":"2016"},{"issue":"7","key":"ref31","first-page":"315","article-title":"Caenorhabditis elegans reveals novel Pseudomonas aeruginosa virulence mechanism","volume":"21","author":"PD Utari","year":"2013"},{"key":"ref32","article-title":"Red seaweeds Sarcodiothecagaudichaudii and Chondruscrispus down regulate virulence factors of Salmonella Enteritidis and induce immune responses in Caenorhabditis elegans","volume":"7","author":"G Kulshreshtha","year":"2016"},{"key":"ref33","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01586-17","article-title":"Interplay among resistance profiles, high-risk clones and virulence in the Caenorhabditis elegans Pseudomonas aeruginosa infection model","author":"I S\u00e1nchez-Diener","year":"2017"},{"issue":"2","key":"ref34","first-page":"203","article-title":"Probiotic Enterococcus faecalisSymbioflor<sup>\u00ae<\/sup> down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model","volume":"199","author":"K Neuhaus","year":"2017"},{"key":"ref35","article-title":"Decrease of Staphylococcus aureus Virulence by Helcococcuskunzii in a Caenorhabditis elegans Model","volume":"7","author":"CN Essebe","year":"2017"},{"key":"ref36","first-page":"72","article-title":"Association between the agr locus and the presence of virulence genes and pathogenesis in Staphylococcus aureus using a Caenorhabditis elegans model","volume":"54","author":"TA Thompson","year":"2017"},{"issue":"4","key":"ref37","first-page":"1137","article-title":"Natural genetic variation in the Caenorhabditis elegans response to Pseudomonas aeruginosa","volume":"7","author":"N Martin","year":"2017"},{"key":"ref38","first-page":"1","article-title":"Local and long-range activation of innate immunity by infection and damage in C. elegans","volume":"38","author":"JJ Ewbank","year":"2016"},{"issue":"4","key":"ref39","first-page":"244","article-title":"GATA transcription factor as a likely key regulator of the Caenorhabditis elegans innate immune response against gut pathogens","volume":"119","author":"W Yang","year":"2016"},{"issue":"5","key":"ref40","first-page":"1776","article-title":"Indoleassociated predator\u2013prey interactions between the nematode Caenorhabditis elegans and bacteria","volume":"19","author":"JH Lee","year":"2017"},{"issue":"3","key":"ref41","first-page":"269","article-title":"RhoA activation by CNFy restores cell\u2013cell adhesion in kindlin2deficient keratinocytes","volume":"233","author":"Y He","year":"2014"},{"issue":"2","key":"ref42","first-page":"181","article-title":"Shiga Toxin Subtypes and Virulence Genes in Escherichia coli Isolated from Cattle","volume":"70","author":"Y Akiyama","year":"2017"},{"key":"ref43","article-title":"Bacterial exotoxins and the inflammasome","volume":"6","author":"AJ Greaney","year":"2015"},{"key":"ref44","article-title":"Bacterial Exotoxins: How Bacteria Fight the Immune System","volume":"7","author":"I Sastalla","year":"2016"},{"key":"ref45","first-page":"73","article-title":"Thermal inactivation responses of acid adapted and non-adapted stationary phase Shiga toxin-producing Escherichia coli (STEC), Salmonella spp. and Listeria monocytogenes in orange juice","volume":"72","author":"Z Topalcengiz","year":"2017"},{"issue":"42","key":"ref46","first-page":"E4458","article-title":"Feedback regulation via AMPK and HIF-1 mediates ROS-dependent longevity in Caenorhabditis elegans","volume":"111","author":"AB Hwang","year":"2014"},{"key":"ref47","first-page":"1707","article-title":"High-Throughput Genetic Screen Reveals that Early Attachment and Biofilm Formation Are Necessary for Full Pyoverdine Production by Pseudomonas aeruginosa","volume":"8","author":"D Kang","year":"2017"},{"issue":"340","key":"ref48","first-page":"340ra72","article-title":"Amyloid-\u03b2 peptide protects against microbial infection in mouse and worm models of Alzheimer\u2019s disease","volume":"8","author":"DKV Kumar","year":"2016"},{"key":"ref49","article-title":"Emerging types of Shiga toxin-producing E. coli (STEC) O178 present in cattle, deer, and humans from Argentina and Germany","volume":"4","author":"A Miko","year":"2014"},{"key":"ref50","first-page":"20","article-title":"Virulence characterization of non-O157 Shiga toxin-producing Escherichia coli isolates from food, humans and animals","volume":"50","author":"J Shen","year":"2015"},{"key":"ref51","first-page":"8","article-title":"Whole genome sequencing of diverse Shiga toxin-producing and non-producing Escherichia coli strains reveals a variety of virulence and novel antibiotic resistance plasmids","volume":"83","author":"L Losada","year":"2016"},{"issue":"7","key":"ref52","first-page":"697","article-title":"Molecular epidemiological view on Shiga toxin-producing Escherichia coli causing human disease in Germany: Diversity, prevalence, and outbreaks","volume":"305","author":"A Fruth","year":"2015"},{"issue":"3","key":"ref53","article-title":"Applying phylogenomics to understand the emergence of Shiga-toxin-producing Escherichia coli O157: H7 strains causing severe human disease in the UK","volume":"1","author":"TJ Dallman","year":"2015"},{"issue":"2","key":"ref54","first-page":"105","article-title":"Shiga toxins\u2014from cell biology to biomedical applications","volume":"8","author":"L Johannes","year":"2010"},{"issue":"1","key":"ref55","first-page":"1","article-title":"Construction of a novel bioluminescent reporter system for investigating Shiga toxin expression of enterohemorrhagic Escherichia coli","volume":"478","author":"T Shimizu","year":"2011"},{"key":"ref56","article-title":"The C. elegans Research Community","author":"T Stiernagle","year":"2006"},{"issue":"7","key":"ref57","first-page":"291","article-title":"Predicting longevity in C. elegans: fertility, mobility and gene expression","volume":"134","author":"JR Cypser","year":"2013"},{"issue":"20","key":"ref58","first-page":"6404","article-title":"Influence of lactic acid bacteria on longevity of Caenorhabditis elegans and host defense against Salmonella entericaserovarenteritidis","volume":"73","author":"T Ikeda","year":"2007"},{"issue":"3","key":"ref59","first-page":"261","article-title":"Visualizing hidden heterogeneity in isogenic populations of C. elegans","volume":"41","author":"D Wu","year":"2006"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0193277","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T12:34:48Z","timestamp":1720096488000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0193277"}},"subtitle":[],"editor":[{"given":"Myon-Hee","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,2,28]]},"references-count":59,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,2,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0193277","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,28]]}}}