{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:00:43Z","timestamp":1790665243096,"version":"4.1.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T00:00:00Z","timestamp":1520380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["1KL2TR001109"],"award-info":[{"award-number":["1KL2TR001109"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["1UL1TR001111"],"award-info":[{"award-number":["1UL1TR001111"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UNC University Cancer Research Fund"},{"name":"UNC Oncology Clinical Translational Research Training Program","award":["5K12CA120780"],"award-info":[{"award-number":["5K12CA120780"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0193832","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T13:44:54Z","timestamp":1520430294000},"page":"e0193832","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":86,"title":["Immuno-PET imaging of tumor-infiltrating lymphocytes using zirconium-89 radiolabeled anti-CD3 antibody in immune-competent mice bearing syngeneic tumors"],"prefix":"10.1371","volume":"13","author":[{"given":"Denis R.","family":"Beckford Vera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6517-0439","authenticated-orcid":true,"given":"Christof C.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa M.","family":"Bixby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan M.","family":"Glatt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart S.","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoichi","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Y.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan S.","family":"Serody","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin G.","family":"Vincent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew C.","family":"Parrott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,3,7]]},"reference":[{"key":"ref1","first-page":"1467","article-title":"Improved Survival with Ipilimumab in Patients with Metastatic Melanoma","author":"SMO Brien","year":"2012","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1056\/NEJMoa1305133","article-title":"Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma","volume":"369","author":"O Hamid","year":"2013","journal-title":"N Engl J Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1056\/NEJMoa1302369","article-title":"Nivolumab plus ipilimumab in advanced melanoma","volume":"369","author":"JD Wolchok","year":"2013","journal-title":"N Engl J Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1016\/S0140-6736(16)00561-4","article-title":"Atezolizumab in patients with locally advanced and metastatic urothelial carcinoma who have progressed following treatment with platinum-based chemotherapy: A single-arm, multicentre, phase 2 trial","volume":"387","author":"JE Rosenberg","year":"2016","journal-title":"Lancet"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1177\/1756287215597647","article-title":"Targeting PD-1\/PD-L1 in the treatment of metastatic renal cell carcinoma","volume":"7","author":"M Weinstock","year":"2015","journal-title":"Ther Adv Urol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1200\/JCO.2014.58.3708","article-title":"Overall survival and long-term safety of nivolumab (anti-programmed death 1 antibody, BMS-936558, ONO-4538) in patients with previously treated advanced non-small-cell lung cancer","volume":"33","author":"SN Gettinger","year":"2015","journal-title":"J Clin Oncol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1056\/NEJMoa1507643","article-title":"Nivolumab versus Docetaxel in Advanced Nonsquamous Non-Small-Cell Lung Cancer","volume":"373","author":"H Borghaei","year":"2015","journal-title":"N Engl J Med"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/nature13954","article-title":"PD-1 blockade induces responses by inhibiting adaptive immune resistance","volume":"515","author":"P Tumeh","year":"2014","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1182\/blood-2008-12-195792","article-title":"Tumor antigen \u2212 specific CD8 T cells infiltrating the tumor express high levels of PD-1 and are functionally impaired Tumor antigen\u2013specific CD8 T cells infiltrating the tumor express high levels of PD-1 and are functionally impaired","volume":"114","author":"M Ahmadzadeh","year":"2009","journal-title":"Blood"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/bs.ai.2016.02.004","article-title":"Advances in PET Detection of the Antitumor T Cell Response","author":"MN McCracken","year":"2016","journal-title":"Adv Immunol"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nrclinonc.2013.46","article-title":"Treating B-cell cancer with T cells expressing anti-CD19 chimeric antigen receptors","volume":"10","author":"JN Kochenderfer","year":"2013","journal-title":"Nat Rev Clin Oncol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1073\/pnas.1502609112","article-title":"Noninvasive imaging of immune responses","volume":"112","author":"M Rashidian","year":"2015","journal-title":"PNAS"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1038\/nrclinonc.2011.141","article-title":"Noninvasive cell-tracking methods","volume":"8","author":"MF Kircher","year":"2011","journal-title":"Nat Rev Clin Oncol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.2967\/jnumed.114.153338","article-title":"Immuno-PET of Murine T Cell Reconstitution Postadoptive Stem Cell Transplantation Using Anti-CD4 and Anti-CD8 Cys-Diabodies","volume":"56","author":"R Tavar\u00e9","year":"2015","journal-title":"J Nucl Med"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.3390\/molecules18066469","article-title":"<sup>89<\/sup>Zr, a radiometal nuclide with high potential for molecular imaging with PET: Chemistry, applications and remaining challenges","volume":"18","author":"G Fischer","year":"2013","journal-title":"Molecules"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2967\/jnmt.112.111377","article-title":"Review on production of <sup>89<\/sup>Zr in a medical cyclotron for PET radiopharmaceuticals","volume":"41","author":"A Kasbollah","year":"2013","journal-title":"J Nucl Med Technol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.2967\/jnumed.114.149690","article-title":"<sup>89<\/sup>Zr-Labeled Versus 124I-Labeled \u03b1HER2 Fab with Optimized Plasma Half-Life for High-Contrast Tumor Imaging In Vivo","volume":"56","author":"CT Mendler","year":"2015","journal-title":"J Nucl Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1073\/pnas.1316922111","article-title":"Engineered antibody fragments for immuno-PET imaging of endogenous CD8+ T cells in vivo","volume":"111","author":"R Tavar\u00e9","year":"2014","journal-title":"Proc Natl Acad Sci"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1093\/protein\/gzu030","article-title":"Enhanced immunoPET of ALCAM-positive colorectal carcinoma using site-specific <sup>64<\/sup>Cu-DOTA conjugation","volume":"27","author":"R Tavare","year":"2014","journal-title":"Protein Eng Des Sel"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3109\/08923978609031083","article-title":"Target ricin by coupling to an anti-macrophage monoclonal antibody","volume":"8","author":"NE Kaminski","year":"1986","journal-title":"J Immunopharmacol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1634\/stemcells.20-3-215","article-title":"Cell surface antigen and molecular targeting in the treatment of hematologic malignancies","volume":"20","author":"A Countouriotis","year":"2002","journal-title":"Stem Cells"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jim.2013.04.004","article-title":"In vivo depletion of leukocytes and platelets following injection of T cell-specific antibodies into mice","volume":"393","author":"L Loubaki","year":"2013","journal-title":"J Immunol Methods"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1600-065X.1979.tb00289.x","article-title":"Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens","volume":"47","author":"JA Ledbetter","year":"1979","journal-title":"Immunol Rev"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1084\/jem.155.3.914","article-title":"Pillars article: Identification of a T cell-derived B cell growth factor distinct from interleukin 2","volume":"155","author":"M Howard","year":"1982","journal-title":"J. Exp. Med"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1038\/312548a0","article-title":"Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo","volume":"312","author":"SP Cobbold","journal-title":"Nature"},{"issue":"10","key":"ref26","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.2967\/jnumed.116.173930","article-title":"Quantitative CD3 PET Imaging Predicts Tumor Growth Response to Anti-CTLA-4 Therapy","volume":"57","author":"BM Larimer","year":"2016","journal-title":"J Nuc Med"},{"key":"ref27","first-page":"15","article-title":"Principles of Immunopharmacology","author":"F Broere","year":"2011","journal-title":"."},{"key":"ref28","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/nri1842","article-title":"Adoptive immunotherapy for cancer: building on success","volume":"6","author":"L Gattinoni","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1016\/j.addr.2008.04.006","article-title":"Bifunctional coupling agents for radiolabeling of biomolecules and target-specific delivery of metallic radionuclides","volume":"60","author":"S Liu","year":"2008","journal-title":"Adv Drug Deliv Rev"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1039\/C3CS60304K","article-title":"Matching chelators to radiometals for radiopharmaceuticals","volume":"43","author":"EW Price","year":"2014","journal-title":"Chem Soc Rev"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/nprot.2010.13","article-title":"Conjugation and radiolabeling of monoclonal antibodies with Zirconium-89 for PET imaging using the bifunctional chelate p-isothiocyanatobenzyl-desferrioxamine","volume":"5","author":"MJWD Vosjan","year":"2010","journal-title":"Nat Protoc"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/nprot.2010.13","article-title":"Conjugation and radiolabeling of monoclonal antibodies with Zirconium-89 for PET imaging using the bifunctional chelate p-isothiocyanatobenzyl-desferrioxamine","volume":"5","author":"MJWD Vosjan","year":"2010","journal-title":"Nat Protoc"},{"key":"ref33","first-page":"1","article-title":"The Bioconjugation and Radiosynthesis of <sup>89<\/sup>Zr-DFO-labeled Antibodies","volume":"1","author":"BM Zeglis","year":"2015","journal-title":"J Vis Exp"},{"key":"ref34","first-page":"1","article-title":"Development and characterization of <sup>89<\/sup>Zr-labeled panitumumab for immuno-positron emission tomographic imaging of the epidermal growth factor receptor","volume":"12","author":"AJ Chang","year":"2013","journal-title":"Mol Imaging"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1158\/0008-5472.CAN-06-3388","article-title":"High number of intraepithelial CD8+ tumor-infiltrating lymphocytes is associated with the absence of lymph node metastases in patients with large early-stage cervical cancer","volume":"67","author":"SJ Piersma","year":"2007","journal-title":"Cancer Res"},{"issue":"7","key":"ref36","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s00262-003-0388-5","article-title":"Prognostic value of the CD4+\/CD8+ ratio of tumour infiltrating lymphocytes in colorectal cancer and HLA-DR expression on tumour cells","volume":"52","author":"AC Diederichsen","year":"2003","journal-title":"Cancer Immunol Immunother"},{"issue":"5","key":"ref37","first-page":"1463","article-title":"Phenotypic analysis of tumor-infiltrating lymphocytes from human breast cancer","volume":"12","author":"Y Chin","year":"1992","journal-title":"Anticancer Res"},{"key":"ref38","first-page":"399","article-title":"Mutagenicity of N -Butyl- N\u2014 (4-hydroxybutyl) nitrosamine, a Bladder Carcinogen, and Related Compounds","author":"M Nagao","year":"1977"},{"key":"ref39","first-page":"2813","article-title":"The pathogenesis of experimental bladder cancer","volume":"37","author":"GT Bryan","year":"1977","journal-title":"Cancer Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0193832","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T05:17:25Z","timestamp":1540271845000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0193832"}},"subtitle":[],"editor":[{"given":"Hiroshi","family":"Shiku","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,3,7]]},"references-count":39,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,3,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0193832","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,7]]}}}