{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:32:11Z","timestamp":1772202731846,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T00:00:00Z","timestamp":1521676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Roche Diagnostics (FR)"},{"name":"CERBA HealthCare (FR)"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0194396","type":"journal-article","created":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T13:41:10Z","timestamp":1521726070000},"page":"e0194396","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of the cobas\u00ae GT hepatitis C virus genotyping assay in G1-6 viruses including low viral loads and LiPA failures"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3843-9380","authenticated-orcid":true,"given":"Benjamin","family":"N\u00e9moz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e9a","family":"Roger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Leroy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Dominique","family":"Poveda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Morand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Larrat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,3,22]]},"reference":[{"key":"ref1","unstructured":"WHO. Global hepatitis report, 2017 [Internet]. 2017 apr. Report No.: ISBN: 978-92-4-156545-5. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/hepatitis\/publications\/global-hepatitis-report2017\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/hepatitis\/publications\/global-hepatitis-report2017\/en\/<\/ext-link>"},{"issue":"9963","key":"ref2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0140-6736(14)61682-2","article-title":"Global, regional, and national age\u2013sex specific all-cause and cause-specific mortality for 240 causes of death, 1990\u20132013: a systematic analysis for the Global Burden of Disease Study 2013","volume":"385","year":"2015","journal-title":"The Lancet"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.jhep.2015.03.025","article-title":"EASL Recommendations on Treatment of Hepatitis C 2015","volume":"63","year":"2015","journal-title":"J Hepatol"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1128\/JCM.02390-07","article-title":"Evaluation of Versant Hepatitis C Virus Genotype Assay (LiPA) 2.0","volume":"46","author":"J Verbeeck","year":"2008","journal-title":"J Clin Microbiol"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.jcv.2006.11.007","article-title":"Evaluation of the Abbott RealTime HCV assay for quantitative detection of hepatitis C virus RNA","volume":"38","author":"BDA Michelin","year":"2007","journal-title":"J Clin Virol"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"4855","DOI":"10.1128\/JCM.41.10.4855-4857.2003","article-title":"Evaluation of the TRUGENE HCV 5\u2019NC Genotyping Kit with the New GeneLibrarian Module 3.1.2 for Genotyping of Hepatitis C Virus from Clinical Specimens","volume":"41","author":"JJ Germer","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ref7","doi-asserted-by":"crossref","first-page":"e0153754","DOI":"10.1371\/journal.pone.0153754","article-title":"Using NS5B Sequencing for Hepatitis C Virus Genotyping Reveals Discordances with Commercial Platforms","volume":"11","author":"N Chueca","year":"2016","journal-title":"PLoS One"},{"issue":"9","key":"ref8","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1128\/JCM.00586-13","article-title":"Sequencing Assays for Failed Genotyping with the Versant Hepatitis C Virus Genotype Assay (LiPA), Version 2.0","volume":"51","author":"S Larrat","year":"2013","journal-title":"J Clin Microbiol"},{"issue":"43","key":"ref9","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES2014.19.43.20944","article-title":"Recent evidence of underestimated circulation of hepatitis C virus intergenotypic recombinant strain RF2k\/1b in the Rh\u00f4ne-Alpes region, France, January to August 2014: implications for antiviral treatment","volume":"19","author":"C Rami\u00e8re","year":"2014","journal-title":"Eurosurveillance"},{"issue":"12","key":"ref10","doi-asserted-by":"crossref","first-page":"e8209","DOI":"10.1371\/journal.pone.0008209","article-title":"Hepatitis C Virus (HCV) Genotype 1 Subtype Identification in New HCV Drug Development and Future Clinical Practice","volume":"4","author":"S Chevaliez","year":"2009","journal-title":"PLoS One"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1128\/JCM.02193-16","article-title":"Multicenter comparison study of both analytical and clinical performance across 4 Roche HCV RNA assays utilizing different platforms","volume":"55","author":"J Vermehren","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1515\/cclm-2016-0620","article-title":"Evaluation of the new cobas<sup>\u00ae<\/sup> HCV genotyping test based on real-time PCRs of three different HCV genome regions","volume":"55","author":"E Stelzl","year":"2017","journal-title":"Clin Chem Lab Med"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jcv.2015.03.023","article-title":"Evaluation of the COBAS<sup>\u00ae<\/sup> AmpliPrep\/COBAS<sup>\u00ae<\/sup> TaqMan<sup>\u00ae<\/sup> HCV Test, v2.0 and comparison to assays used in routine clinical practice in an international multicenter clinical trial: The ExPECT study","volume":"67","author":"HH Kessler","year":"2015","journal-title":"J Clin Virol"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jviromet.2012.06.011","article-title":"Genotyping and resistance profile of hepatitis C (HCV) genotypes 1\u20136 by sequencing the NS3 protease region using a single optimized sensitive method","volume":"185","author":"B Besse","year":"2012","journal-title":"J Virol Methods"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0166-0934(03)00070-3","article-title":"Determining hepatitis C genotype by analyzing the sequence of the NS5b region","volume":"109","author":"K Sandres-Saun\u00e9","year":"2003","journal-title":"J Virol Methods"},{"issue":"2\u20133","key":"ref16","first-page":"153","article-title":"High prevalence of Hepatitis C virus genotype 6 in Vietnam","volume":"27","author":"DA Pham","year":"2009","journal-title":"Asian Pac J Allergy Immunol"},{"issue":"1\u20132","key":"ref17","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/10665270050081478","article-title":"A Greedy Algorithm for Aligning DNA Sequences","volume":"7","author":"Z Zhang","year":"2000","journal-title":"J Comput Biol. feb"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"e0155869","DOI":"10.1371\/journal.pone.0155869","article-title":"Geno2pheno[HCV]\u2013A Web-based Interpretation System to Support Hepatitis C Treatment Decisions in the Era of Direct-Acting Antiviral Agents","volume":"11","author":"P Kalaghatgi","year":"2016","journal-title":"PLoS One"},{"issue":"W1","key":"ref19","doi-asserted-by":"crossref","first-page":"W22","DOI":"10.1093\/nar\/gkt389","article-title":"aLeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity","volume":"41","author":"S Kuraku","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1128\/JCM.02366-06","article-title":"Use of sequence analysis of the NS5B region for routine genotyping of Hepatitis C Virus with reference to C\/E1 and 5\u2019 untranslated region sequences","volume":"45","author":"DG Murphy","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref21","unstructured":"Roche Diagnostics. Package insert: cobas<sup>\u00ae<\/sup> HCV GT [Internet]. Report No.: 07564414001-01FR. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/molecular.roche.com\/assays\/cobas-hcv-gt-for-use-on-the-cobas-4800-system\/\" xlink:type=\"simple\">https:\/\/molecular.roche.com\/assays\/cobas-hcv-gt-for-use-on-the-cobas-4800-system\/<\/ext-link>"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/JCM.01960-16","article-title":"Assessment of a novel automatic real-time PCR assay on the cobas 4800 analyzer as a screening platform for hepatitis C virus genotyping in clinical practice: comparison with massive sequencing","volume":"55","author":"L Nieto-Aponte","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1371\/journal.pone.0175564","article-title":"The cobas<sup>\u00ae<\/sup> HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice","volume":"12","author":"JA Fern\u00e1ndez-Caballero","year":"2017","journal-title":"PLoS One"},{"issue":"11","key":"ref24","doi-asserted-by":"crossref","first-page":"947.e1","DOI":"10.1016\/j.cmi.2016.07.032","article-title":"First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype","volume":"22","author":"M Salmona","year":"2016","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1128\/JCM.02831-14","article-title":"Hepatitis C Virus Genotype 7, a New Genotype Originating from Central Africa","volume":"53","author":"DG Murphy","year":"2015","journal-title":"J Clin Microbiol"},{"key":"ref26","unstructured":"Hedskog C. Identification of novel HCV genotype and subtypes in patients treated with sofosbuvir based regimens\u2014C. AASLD, abstract 210. oct 2017; <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/liverlearning.aasld.org\/aasld\/2017\/thelivermeeting\/201395\/charlotte.hedskog.identification.of.novel.hcv.genotype.and.subtypes.in.html\" xlink:type=\"simple\">https:\/\/liverlearning.aasld.org\/aasld\/2017\/thelivermeeting\/201395\/charlotte.hedskog.identification.of.novel.hcv.genotype.and.subtypes.in.html<\/ext-link>"},{"key":"ref27","article-title":"Efficacy and safety of glecaprevir\/pibrentasvir in HCV-infected Japanese patients with prior DAA experience, severe renal impairment, or genotype 3 infection","author":"H Kumada","year":"2017","journal-title":"J Gastroenterol"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jhep.2017.03.039","article-title":"Glecaprevir and pibrentasvir yield high response rates in patients with HCV genotype 1\u20136 without cirrhosis","volume":"67","author":"PY Kwo","year":"2017","journal-title":"J Hepatol"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1053\/j.gastro.2017.03.047","article-title":"Efficacy of 8 Weeks of Sofosbuvir, Velpatasvir, and Voxilaprevir in Patients With Chronic HCV Infection: 2 Phase 3 Randomized Trials","volume":"153","author":"IM Jacobson","year":"2017","journal-title":"Gastroenterology"},{"issue":"11","key":"ref30","doi-asserted-by":"crossref","first-page":"6807","DOI":"10.1128\/AAC.01229-15","article-title":"Hepatitis C Virus Genotype 4 Resistance and Subtype Demographic Characterization of Patients Treated with Ombitasvir plus Paritaprevir\/Ritonavir","volume":"59","author":"G Schnell","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1002\/hep.28611","article-title":"Hepatitis C genotype 4R resistance-associated polymorphisms: The achilles heel of the nonstructural 5A inhibitors?","volume":"64","author":"P Halfon","year":"2016","journal-title":"Correspondence. Hepatology"},{"key":"ref32","unstructured":"AASLD. Recommendations for Testing, Managing, and Treating Hepatitis C. Aasld. 2016;1\u201351. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/hcvguidelines.org\/sites\/default\/files\/HCV-Guidance_July_2016_b.pdf\" xlink:type=\"simple\">http:\/\/hcvguidelines.org\/sites\/default\/files\/HCV-Guidance_July_2016_b.pdf<\/ext-link>"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0194396","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:54:49Z","timestamp":1540342489000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0194396"}},"subtitle":[],"editor":[{"given":"Ming-Lung","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,3,22]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,3,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0194396","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.732887826.793544225","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,22]]}}}