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Resistance to the crayfish plague in some American, Japanese and European crayfishes. Drottningholm: 1969."},{"key":"ref5","first-page":"182","article-title":"Freshwater Crayfish: A Global Overview","author":"S Rezinciuc","year":"2015"},{"issue":"5452","key":"ref6","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1126\/science.287.5452.443","article-title":"Emerging Infectious Diseases of Wildlife\u2014Threats to Biodiversity and Human Health","volume":"287","author":"P Daszak","year":"2000","journal-title":"Sci"},{"key":"ref7","first-page":"334","article-title":"Sulla malattia dei gamberi","volume":"2","author":"E Cornalia","year":"1860","journal-title":"Atti della Societ\u00e0 italiana di scienze naturali"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1899\/10-130.1","article-title":"Haplotype variation in the spiny-cheek crayfishOrconectes limosus: colonization of Europe and genetic diversity of native stocks","volume":"30","author":"L Filipov\u00e1","year":"2011","journal-title":"Journal of the North American Benthological Society"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0044-8486(94)90243-7","article-title":"Analysis of genetic diversity in the crayfish plague fungus, Aphanomyces astaci, by random amplification of polymorphic DNA","volume":"126","author":"T-s Huang","year":"1994","journal-title":"Aquaculture"},{"issue":"347","key":"ref10","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1051\/kmae\/1997051","article-title":"The crayfish plague fungus (Aphanomyces astaci) in Spain","author":"J Di\u00e9guez-Uribeondo","year":"1997","journal-title":"Bull Fr P\u00eache Piscic"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0006-3207(93)90076-D","article-title":"Crayfish plague and management strategies in Norway","volume":"63","author":"T Taugb\u00f8l","year":"1993","journal-title":"Biol Conserv"},{"issue":"1","key":"ref12","first-page":"266","article-title":"Crayfish plague in Britain, the first twelve years","volume":"9","author":"DJ Alderman","year":"1993","journal-title":"Freshwater Crayfish"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Alderman DJ. History of the spread of crayfish plague in Europe, in Crustaceans: bacterial and fungal diseases OIE, Scientific and Technical Review 1997;15:15\u201323.","DOI":"10.20506\/rst.15.2.943"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF00010773","article-title":"Ecological impact of crayfish plague in Ireland","volume":"234","author":"M Matthews","year":"1992","journal-title":"Hydrobiologia"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1365-2761.1989.tb00292.x","article-title":"Crayfish plague in Turkey","volume":"12","author":"I Baran","year":"1989","journal-title":"Journal of Fish Diseases"},{"key":"ref16","unstructured":"Lowe S, Browne M, Boudjelas S, De Poorter M. 100 of the World\u2019s Worst Invasive Alien Species A selection from the Global Invasive Species Database.: The Invasive Species Specialist Group (ISSG) a specialist group of the Species Survival Commission (SSC) of the World Conservation Union (IUCN). 2000. p. 12."},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/S0953-7562(09)80716-8","article-title":"Physiological adaptation of an Aphanomyces astaci strain isolated from the freshwater crayfish Procambarus clarkii","volume":"99","author":"J Di\u00e9guez-Uribeondo","year":"1995","journal-title":"Mycological Research"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.jip.2011.08.002","article-title":"Spiny-cheek crayfish Orconectes limosus carry a novel genotype of the crayfish plague pathogen Aphanomyces astaci","volume":"108","author":"E Kozubikova","year":"2011","journal-title":"J Invertebr Pathol"},{"issue":"3\u20134","key":"ref19","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.vetmic.2014.02.020","article-title":"Microsatellite markers for direct genotyping of the crayfish plague pathogen Aphanomyces astaci (Oomycetes) from infected host tissues","volume":"170","author":"F 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Mruga\u0142a","year":"2016","journal-title":"Aquatic Conservation: Marine and Freshwater Ecosystems"},{"issue":"8","key":"ref39","first-page":"923","article-title":"Digital Image Analysis of Internal Light Spots of Appressoria of Colletotrichum acutatum","volume":"93","author":"J Di\u00e9guez-Uribeondo","year":"2003","journal-title":"Biochemistry and Cell Biology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"53","DOI":"10.3354\/dao072053","article-title":"Detection of Aphanomyces astaci in North American crayfish by polymerase chain reaction","volume":"72","author":"B Oidtmann","year":"2006","journal-title":"Dis Aquat Organ"},{"issue":"3\u20134","key":"ref41","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.vetmic.2004.01.019","article-title":"Detection of genomic DNA of the crayfish plague fungus Aphanomyces astaci (Oomycete) in clinical samples by PCR","volume":"100","author":"B Oidtmann","year":"2004","journal-title":"Vet Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"36089","DOI":"10.1038\/srep36089","article-title":"Mitochondrial genomes and comparative genomics of Aphanomyces astaci and Aphanomyces invadans","volume":"6","author":"J Makkonen","year":"2016","journal-title":"Sci Rep"},{"issue":"12","key":"ref43","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"M Kearse","year":"2012","journal-title":"Bioinformatics"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"286","DOI":"10.11646\/phytotaxa.270.4.5","article-title":"Saprolegnia milanezii sp. nov., a new species of Saprolegniales (Oomycota, Straminipila) from Brazil","volume":"270","author":"SCO Rocha","year":"2016","journal-title":"Phytotaxa"},{"issue":"1","key":"ref45","first-page":"77","article-title":"The Host-Parasite Relationship between Freshwater Crayfish and the Crayfish Disease Fungus Aphanomyces astaci: Responses to Infection by a Susceptible and a Resistant Species","volume":"60","author":"T UNESTAM","year":"1970","journal-title":"Microbiology"},{"key":"ref46","first-page":"192","article-title":"On the Host Range and Origin of the Crayfish Plague Fungus","volume":"52","author":"T Unestam","year":"1972","journal-title":"Report Institute of the Freshwater Research"},{"issue":"3","key":"ref47","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0022-2011(79)90075-2","article-title":"Attachment of phenoloxidase to fungal cell walls in arthropod immunity","volume":"34","author":"K S\u00f6derh\u00e4ll","year":"1979","journal-title":"Journal of Invertebrate Pathology"},{"key":"ref48","first-page":"32","article-title":"TemaNord","author":"S Viljamaa-Dirks","year":"2008"},{"key":"ref49","first-page":"131","article-title":"The crayfish plague fungus Aphanomyces astaci\u2014diagnosis, isolation, and pathology","volume":"7","author":"L Cerenius","year":"1988","journal-title":"Freshwater Crayfish"},{"key":"ref50","first-page":"761","article-title":"Procambarus clarkii Girard as a vector for the crayfish plague fungus, Aphanomyces astaci Schikora","volume":"24","author":"J Dieguez-Uribeondo","year":"1993","journal-title":"Aquaculture and Fisheries Management"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1051\/kmae:2006036","article-title":"Dispersion of the <italic>Aphanomyces astaci<\/italic>-carrier <italic>Pacifastacus leniusculus<\/italic> by humans represents the main cause of disappearance of the indigenous crayfish of Navarra","volume":"380\u2013381","author":"J Di\u00e9guez-Uribeondo","year":"2006","journal-title":"Bulletin Fran\u00e7ais de la P\u00eache et de la 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