{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:59:20Z","timestamp":1740146360422,"version":"3.37.3"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T00:00:00Z","timestamp":1525910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI024616"],"award-info":[{"award-number":["AI024616"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["Medical Student Fellowship"],"award-info":[{"award-number":["Medical Student Fellowship"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0197010","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T13:33:44Z","timestamp":1525959224000},"page":"e0197010","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of a nontypeable Haemophilus influenzae thermonuclease"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0855-1811","authenticated-orcid":true,"given":"Christine","family":"Cho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5260-3135","authenticated-orcid":true,"given":"Aroon T.","family":"Chande","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lokesh","family":"Gakhar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Hunt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret R.","family":"Ketterer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Apicella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,5,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/clinids\/9.1.1","article-title":"Nontypable <italic>Haemophilus influenzae<\/italic>: a review of clinical aspects, surface antigens and the human response to infection","volume":"9","author":"TF Murphy","year":"1987","journal-title":"Rev Infect Dis"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1164\/rccm.200412-1687OC","article-title":"Haemophilus influenzae from patients with chronic obstructive pulmonary disease exacerbation induce more inflammation than colonizers","volume":"172","author":"CL Chin","year":"2005","journal-title":"Am J Respir Crit Care Med"},{"issue":"12","key":"ref3","doi-asserted-by":"crossref","first-page":"2083","DOI":"10.1097\/00005537-200112000-00001","article-title":"Direct evidence of bacterial biofilms in otitis media","volume":"111","author":"JC Post","year":"2001","journal-title":"Laryngoscope"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1164\/rccm.200509-1459OC","article-title":"Haemophilus influenzae forms biofilms on airway epithelia: implications in cystic fibrosis","volume":"174","author":"TD Starner","year":"2006","journal-title":"Am J Respir Crit Care Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1146\/annurev.micro.56.012302.160705","article-title":"Biofilms as complex differentiated communities","volume":"56","author":"P Stoodley","year":"2002","journal-title":"Annu Rev Microbiol"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"6851","DOI":"10.1128\/AAC.03514-14","article-title":"Enhanced in vitro formation and antibiotic resistance of nonattached Pseudomonas aeruginosa aggregates through incorporation of neutrophil products","volume":"58","author":"SM Caceres","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"issue":"13","key":"ref7","doi-asserted-by":"crossref","DOI":"10.1093\/femsle\/fnx120","article-title":"Matrix exopolysaccharides; the sticky side of biofilm formation","volume":"364","author":"E Maunders","year":"2017","journal-title":"FEMS Microbiol Lett"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S0966-842X(00)01913-2","article-title":"Mechanisms of biofilm resistance to antimicrobial agents","volume":"9","author":"TF Mah","year":"2001","journal-title":"Trends Microbiol"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1128\/IAI.02601-14","article-title":"Role of the nuclease of nontypeable Haemophilus influenzae in dispersal of organisms from biofilms","volume":"83","author":"C Cho","year":"2015","journal-title":"Infect Immun"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1006\/abio.1994.1353","article-title":"A fluorometric assay for DNA cleavage reactions characterized with BamHI restriction endonuclease","volume":"220","author":"SP Lee","year":"1994","journal-title":"Anal Biochem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.aca.2012.06.032","article-title":"A graphene-based real-time fluorescent assay of deoxyribonuclease I activity and inhibition","volume":"740","author":"Z Zhou","year":"2012","journal-title":"Analytica chimica acta"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1038\/nprot.2006.202","article-title":"Using circular dichroism spectra to estimate protein secondary structure","volume":"1","author":"NJ Greenfield","year":"2006","journal-title":"Nature protocols"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1107\/S0021889803012779","article-title":"PRIMUS: a Windows PC-based system for small-angle scattering data analysis","volume":"36","author":"P Konarev","year":"2003","journal-title":"Journal of Applied Crystallography"},{"year":"1955","author":"A Guinier","key":"ref14"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1107\/S002188989100081X","article-title":"GNOM-a program package for small-angle scattering data processing","volume":"24.5","author":"A Semenyuk","year":"1991","journal-title":"Journal of Applied Crystallography"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1107\/S0021889895007047","article-title":"CRYSOL-a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates","volume":"28.6","author":"D Svergun","year":"1995","journal-title":"Journal of Applied Crystallography"},{"issue":"6","key":"ref17","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1016\/S0006-3495(99)77443-6","article-title":"Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing","volume":"76","author":"DI Svergun","year":"1999","journal-title":"Biophysical journal"},{"issue":"6","key":"ref18","doi-asserted-by":"crossref","first-page":"2946","DOI":"10.1016\/S0006-3495(01)76260-1","article-title":"Determination of domain structure of proteins from X-ray solution scattering","volume":"80","author":"DI Svergun","year":"2001","journal-title":"Biophysical journal"},{"key":"ref19","article-title":"Automated matching of high-and low-resolution structural models","volume":"34.1","author":"MB Kozin","year":"2001","journal-title":"Journal of Applied Crystallography"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1107\/S0021889803000268","article-title":"Uniqueness of ab initio shape determination in small-angle scattering","volume":"36.3","author":"V Volkov","year":"2003","journal-title":"Journal of Applied Crystallography"},{"issue":"9","key":"ref21","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1002\/pro.5560050917","article-title":"Coupling between trans\/cis proline isomerization and protein stability in staphylococcal nuclease","volume":"5","author":"DM Truckses","year":"1996","journal-title":"Protein Sci"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/BF00226675","article-title":"Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding","volume":"23","author":"PW Tucker","year":"1979","journal-title":"Mol Cell Biochem"},{"issue":"13","key":"ref23","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.1021\/bi00013a021","article-title":"X-ray crystal structures of staphylococcal nuclease complexed with the competitive inhibitor cobalt(II) and nucleotide","volume":"34","author":"PJ Loll","year":"1995","journal-title":"Biochemistry"},{"issue":"15","key":"ref24","doi-asserted-by":"crossref","first-page":"3960","DOI":"10.1111\/j.1742-4658.2005.04814.x","article-title":"Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding","volume":"272","author":"Z Su","year":"2005","journal-title":"Febs J"},{"issue":"22","key":"ref25","doi-asserted-by":"crossref","first-page":"6044","DOI":"10.1021\/bi00343a004","article-title":"Thermal denaturation of staphylococcal nuclease","volume":"24","author":"RO Calderon","year":"1985","journal-title":"Biochemistry"},{"issue":"1","key":"ref26","first-page":"27","article-title":"Micrococcal nuclease and some products of its action","volume":"3","author":"L Cunningham","year":"1958","journal-title":"Ann N Y Acad Sci"},{"key":"ref27","article-title":"The Neisseria gonorrhoeae Biofilm Matrix Contains DNA and an Endogenous Nuclease Controls its Incorporation","author":"CT Steichen","year":"2011","journal-title":"Infection and Immunity"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1128\/IAI.01242-12","article-title":"Staphylococcus aureus nuclease is an SaeRS-dependent virulence factor","volume":"81","author":"ME Olson","year":"2013","journal-title":"Infection and Immunity"},{"issue":"8","key":"ref29","doi-asserted-by":"crossref","first-page":"e103125","DOI":"10.1371\/journal.pone.0103125","article-title":"Cell wall-anchored nuclease of Streptococcus sanguinis contributes to escape from neutrophil extracellular trap-mediated bacteriocidal activity","volume":"9","author":"C Morita","year":"2014","journal-title":"PLoS One"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1093\/infdis\/jiv031","article-title":"A thermonuclease of Neisseria gonorrhoeae enhances bacterial escape from killing by neutrophil extracellular traps","volume":"212","author":"RA Juneau","year":"2015","journal-title":"J Infect Dis"},{"issue":"6160","key":"ref31","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1126\/science.1242255","article-title":"Staphylococcus aureus degrades neutrophil extracellular traps to promote immune cell death","volume":"342","author":"V Thammavongsa","year":"2013","journal-title":"Science"},{"issue":"15","key":"ref32","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1021\/bi00229a005","article-title":"Deletion of the omega-loop in the active site of staphylococcal nuclease. 1. Effect on catalysis and stability","volume":"30","author":"LB Poole","year":"1991","journal-title":"Biochemistry"},{"issue":"15","key":"ref33","doi-asserted-by":"crossref","first-page":"3628","DOI":"10.1021\/bi00229a006","article-title":"Deletion of the omega-loop in the active site of staphylococcal nuclease. 2. Effects on protein structure and dynamics","volume":"30","author":"DM Baldisseri","year":"1991","journal-title":"Biochemistry"},{"issue":"5559","key":"ref34","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1126\/science.295.5559.1487","article-title":"Extracellular DNA required for bacterial biofilm formation","volume":"295","author":"CB Whitchurch","year":"2002","journal-title":"Science"},{"issue":"4","key":"ref35","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/mbo3.187","article-title":"The biofilm matrix destabilizers, EDTA and DNaseI, enhance the susceptibility of nontypeable Hemophilus influenzae biofilms to treatment with ampicillin and ciprofloxacin","volume":"3","author":"R Cavaliere","year":"2014","journal-title":"Microbiologyopen"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1021\/jf072803c","article-title":"Studies on umami taste. Synthesis of new guanosine 5&apos;-phosphate derivatives and their synergistic effect with monosodium glutamate","volume":"56","author":"P Cairoli","year":"2008","journal-title":"J Agric Food Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"989","DOI":"10.3389\/fmicb.2016.00989","article-title":"Identification of the agr Peptide of Listeria monocytogenes","volume":"7","author":"M Zetzmann","year":"2016","journal-title":"Front Microbiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0197010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T10:52:20Z","timestamp":1540032740000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0197010"}},"subtitle":[],"editor":[{"given":"William M.","family":"Shafer","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,5,10]]},"references-count":37,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2018,5,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0197010","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2018,5,10]]}}}