{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T20:49:01Z","timestamp":1762980541754,"version":"3.37.3"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T00:00:00Z","timestamp":1525824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H06536, 17H05010"],"award-info":[{"award-number":["16H06536, 17H05010"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0197128","type":"journal-article","created":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T17:40:03Z","timestamp":1525887603000},"page":"e0197128","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing"],"prefix":"10.1371","volume":"13","author":[{"given":"Hiroyuki","family":"Obinata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asako","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8367-9228","authenticated-orcid":true,"given":"Shinsuke","family":"Niwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,5,9]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"3959","DOI":"10.1002\/j.1460-2075.1991.tb04966.x","article-title":"Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences","volume":"10","author":"CC Mello","year":"1991","journal-title":"EMBO J"},{"issue":"11","key":"ref2","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1007\/s00018-011-0670-1","article-title":"Selectable genetic markers for nematode transgenesis","volume":"68","author":"R Giordano-Santini","year":"2011","journal-title":"Cell Mol Life Sci"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1093\/genetics\/141.3.977","article-title":"Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system","volume":"141","author":"M Maduro","year":"1995","journal-title":"Genetics"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1093\/nar\/22.9.1762","article-title":"pha-1, a selectable marker for gene transfer in C. elegans","volume":"22","author":"M Granato","year":"1994","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"4556","DOI":"10.1128\/MCB.18.8.4556","article-title":"Caenorhabditis elegans SUR-5, a novel but conserved protein, negatively regulates LET-60 Ras activity during vulval induction","volume":"18","author":"T Gu","year":"1998","journal-title":"Mol Cell Biol"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1038\/ng.248","article-title":"Single-copy insertion of transgenes in Caenorhabditis elegans","volume":"40","author":"C Frokjaer-Jensen","year":"2008","journal-title":"Nat Genet"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1128\/MCB.5.12.3484","article-title":"Extrachromosomal DNA transformation of Caenorhabditis elegans","volume":"5","author":"DT Stinchcomb","year":"1985","journal-title":"Mol Cell Biol"},{"issue":"9","key":"ref8","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nmeth.1495","article-title":"Rapid selection of transgenic C. elegans using antibiotic resistance","volume":"7","author":"JI Semple","year":"2010","journal-title":"Nat Methods"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1038\/nmeth.1494","article-title":"An antibiotic selection marker for nematode transgenesis","volume":"7","author":"R Giordano-Santini","year":"2010","journal-title":"Nat Methods"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","first-page":"e76019","DOI":"10.1371\/journal.pone.0076019","article-title":"Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection","volume":"8","author":"I Radman","year":"2013","journal-title":"PLoS One"},{"key":"ref11","article-title":"Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos","author":"S Namai","year":"2018","journal-title":"Dev Genes Evol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1534\/genetics.114.166389","article-title":"A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans","volume":"197","author":"H Kim","year":"2014","journal-title":"Genetics"},{"issue":"11","key":"ref13","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.7164\/antibiotics.31.1137","article-title":"Streptothricin F, an inhibitor of protein synthesis with miscoding activity","volume":"31","author":"I Haupt","year":"1978","journal-title":"J Antibiot (Tokyo)"},{"issue":"14","key":"ref14","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1002\/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K","article-title":"Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae","volume":"15","author":"AL Goldstein","year":"1999","journal-title":"Yeast"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1364-3703.2012.00826.x","article-title":"Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana","volume":"14","author":"B Ramos","year":"2013","journal-title":"Mol Plant Pathol"},{"issue":"7","key":"ref16","doi-asserted-by":"crossref","first-page":"e68509","DOI":"10.1371\/journal.pone.0068509","article-title":"Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells","volume":"8","author":"BS Kochupurakkal","year":"2013","journal-title":"PLoS One"},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1093\/nar\/18.5.1283","article-title":"Nucleotide sequence of the streptothricin-acetyl-transferase gene sat-2","volume":"18","author":"E Tietze","year":"1990","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1038\/nmeth.2641","article-title":"Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination","volume":"10","author":"DJ Dickinson","year":"2013","journal-title":"Nat Methods"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1534\/genetics.115.178335","article-title":"Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette","volume":"200","author":"DJ Dickinson","year":"2015","journal-title":"Genetics"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","article-title":"The genetics of Caenorhabditis elegans","volume":"77","author":"S Brenner","year":"1974","journal-title":"Genetics"},{"issue":"2","key":"ref21","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nmeth.1865","article-title":"Improved Mos1-mediated transgenesis in C. elegans","volume":"9","author":"C Frokjaer-Jensen","year":"2012","journal-title":"Nat Methods"},{"issue":"7","key":"ref22","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1093\/bioinformatics\/btu743","article-title":"CRISPRdirect: software for designing CRISPR\/Cas guide RNA with reduced off-target sites","volume":"31","author":"Y Naito","year":"2015","journal-title":"Bioinformatics"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/tra.12142","article-title":"In vivo neuron-wide analysis of synaptic vesicle precursor trafficking","volume":"15","author":"CI Maeder","year":"2014","journal-title":"Traffic"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nmeth.1318","article-title":"Enzymatic assembly of DNA molecules up to several hundred kilobases","volume":"6","author":"DG Gibson","year":"2009","journal-title":"Nat Methods"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/nmeth.2889","article-title":"Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon","volume":"11","author":"C Frokjaer-Jensen","year":"2014","journal-title":"Nat Methods"},{"issue":"17","key":"ref26","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1016\/j.cub.2017.07.013","article-title":"BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8","volume":"27","author":"S Niwa","year":"2017","journal-title":"Curr Biol"},{"issue":"7","key":"ref27","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.cell.2013.12.001","article-title":"Dynamic imaging of genomic loci in living human cells by an optimized CRISPR\/Cas system","volume":"155","author":"B Chen","year":"2013","journal-title":"Cell"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1534\/genetics.114.172361","article-title":"Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair","volume":"199","author":"JD Ward","year":"2015","journal-title":"Genetics"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1534\/genetics.114.169730","article-title":"Efficient marker-free recovery of custom genetic modifications with CRISPR\/Cas9 in Caenorhabditis elegans","volume":"198","author":"JA Arribere","year":"2014","journal-title":"Genetics"},{"issue":"17","key":"ref30","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.253","article-title":"The Method of the Body Bending Assay Using Caenorhabditis elegans","volume":"2","author":"M Nawa","year":"2012","journal-title":"bio-protocol"},{"issue":"128","key":"ref31","doi-asserted-by":"crossref","DOI":"10.3791\/56690","article-title":"Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons","author":"S Niwa","year":"2017","journal-title":"J Vis Exp"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/nmeth.1565","article-title":"Codon adaptation-based control of protein expression in C. elegans","volume":"8","author":"S Redemann","year":"2011","journal-title":"Nat Methods"},{"issue":"2","key":"ref33","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1534\/genetics.115.180679","article-title":"Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes","volume":"201","author":"AD Norris","year":"2015","journal-title":"Genetics"},{"issue":"8","key":"ref34","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1016\/j.celrep.2016.07.043","article-title":"Autoinhibition of a Neuronal Kinesin UNC-104\/KIF1A Regulates the Size and Density of Synapses","volume":"16","author":"S Niwa","year":"2016","journal-title":"Cell Rep"},{"issue":"5","key":"ref35","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.neuron.2010.04.033","article-title":"An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation","volume":"66","author":"MP Klassen","year":"2010","journal-title":"Neuron"},{"issue":"2","key":"ref36","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.neuron.2016.09.036","article-title":"Microtubule Organization Determines Axonal Transport Dynamics","volume":"92","author":"S Yogev","year":"2016","journal-title":"Neuron"},{"issue":"2","key":"ref37","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.celrep.2013.09.011","article-title":"Two Wnts instruct topographic synaptic innervation in C. elegans","volume":"5","author":"K Mizumoto","year":"2013","journal-title":"Cell Rep"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"15063","DOI":"10.1038\/ncomms15063","article-title":"Local inhibition of microtubule dynamics by dynein is required for neuronal cargo distribution","volume":"8","author":"S Yogev","year":"2017","journal-title":"Nat Commun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0197128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T00:34:59Z","timestamp":1661214899000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0197128"}},"subtitle":[],"editor":[{"given":"Denis","family":"Dupuy","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,5,9]]},"references-count":38,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2018,5,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0197128","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2018,5,9]]}}}