{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:14:23Z","timestamp":1790403263891,"version":"4.1.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0198021","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T17:34:33Z","timestamp":1527874473000},"page":"e0198021","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":103,"title":["Stimulated and unstimulated saliva samples have significantly different bacterial profiles"],"prefix":"10.1371","volume":"13","author":[{"given":"Sonia","family":"Gomar-Vercher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurea","family":"Sim\u00f3n-Soro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Montiel-Company","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9605-3833","authenticated-orcid":true,"given":"Jos\u00e9 Manuel","family":"Almerich-Silla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Mira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,6,1]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.mimet.2009.09.012","article-title":"Metagenomic study of the oral microbiota by Illumina high-throughput sequencing","volume":"79","author":"V Lazaveric","year":"2009","journal-title":"J Microbiol Methods"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1089\/dna.2009.0874","article-title":"The oral microbiota: living with a permanent guest","volume":"28","author":"M Avila","year":"2009","journal-title":"DNA Cell Biol"},{"issue":"12","key":"ref3","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1099\/jmm.0.47719-0","article-title":"Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease","volume":"57","author":"T Kuehbacher","year":"2008","journal-title":"J Med Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/j.1600-0757.2006.00206.x","article-title":"Nursing home-associated pneumonia, hospital-acquired pneumonia and ventilator-associated pneumonia: the contribution of dental biofilms and periodontal inflammation","volume":"44","author":"K Raghavendran","year":"2000","journal-title":"Periodontol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1128\/JCM.36.1.157-160.1998","article-title":"Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method","volume":"36","author":"J Matto","year":"1998","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1034\/j.1600-051X.2002.290505.x","article-title":"Salivary microbiota levels in relation to periodontal status, experience of caries and miswak use in Sudanese adults","volume":"29","author":"IA Darout","year":"2002","journal-title":"J Clin Periodontol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1046\/j.1472-765X.2003.01262.x","article-title":"Bacteriophage isolation from human saliva","volume":"36","author":"G Bachrach","year":"2003","journal-title":"Lett Appl Microbiol"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1101\/gr.084616.108","article-title":"Global diversity in the human salivary microbiome","volume":"19","author":"I Nasidze","year":"2009","journal-title":"Genome Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1479-5876-3-27","article-title":"The salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects","volume":"3","author":"DL Mager","year":"2005","journal-title":"J Transl Med"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1111\/j.1399-302X.2007.00397.x","article-title":"Microbiological diversity of generalized aggressive periodontitis by 16S rRNA clonal analysis","volume":"23","author":"M Faveri","year":"2008","journal-title":"Oral Microbiol Immunol"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1002\/bmc.438","article-title":"The role of salivary peptides in dental caries","volume":"19","author":"R Vitorino","year":"2005","journal-title":"Biomed Chromatogr"},{"issue":"9","key":"ref12","doi-asserted-by":"crossref","first-page":"4438","DOI":"10.1128\/JCM.41.9.4438-4441.2003","article-title":"Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus","volume":"41","author":"A Yoshida","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1186\/1471-2180-9-259","article-title":"Defining the healthy "core microbiome" of oral microbial communities","volume":"9","author":"E Zaura","year":"2009","journal-title":"BMC Microbiol"},{"key":"ref14","first-page":"196721","article-title":"Identification of microbial and proteomic biomarkers in early childhood caries","volume":"2011","author":"TC Hart","year":"2011","journal-title":"Int J Dent"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.archoralbio.2008.08.007","article-title":"Potential biomarkers of human salivary function: a modified proteomic approach","volume":"54","author":"JD Rudney","year":"2009","journal-title":"Arch Oral Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1755-8794-4-22","article-title":"Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health","volume":"4","author":"W Crielaard","year":"2011","journal-title":"BMC Med Genomics"},{"issue":"1\u20132","key":"ref17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s11010-012-1255-5","article-title":"AP-PCR detection of Streptococcus mutans and Streptococcus sobrinus in caries-free and caries-active subjects","volume":"365","author":"Q Jiang","year":"2012","journal-title":"Mol Cell Biochem"},{"issue":"11","key":"ref18","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1177\/154405910808701104","article-title":"Pyrosequencing analysis of the oral microflora of healthy adults","volume":"87","author":"BJ Keijser","year":"2008","journal-title":"J Dent Res"},{"issue":"1","key":"ref19","first-page":"26","article-title":"Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva","volume":"91","author":"CG Emilson","year":"1983","journal-title":"Scand J Dent Res"},{"issue":"3","key":"ref20","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s00248-010-9712-8","article-title":"Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing","volume":"60","author":"Z Ling","year":"2010","journal-title":"Microb Ecol"},{"issue":"7","key":"ref21","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1177\/0022034513488119","article-title":"Microbial geography of the oral cavity","volume":"92","author":"A Sim\u00f3n-Soro","year":"2013","journal-title":"J Dent Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"30112","DOI":"10.3402\/jom.v8.30112","article-title":"Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples","volume":"8","author":"D Belstr\u00f8m","year":"2016","journal-title":"J Oral Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1472-6831-11-33","article-title":"Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis","volume":"11","author":"S Huang","year":"2011","journal-title":"BMC Oral Health"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1128\/JCM.01824-08","article-title":"Detection of multiple pathogenic species in saliva is associated with periodontal infection in adults","volume":"47","author":"S Paju","year":"2009","journal-title":"J Clin Microbiol"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"e0182992","DOI":"10.1371\/journal.pone.0182992","article-title":"Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients","volume":"12","author":"D Belstr\u00f8m","year":"2017","journal-title":"PLoS One"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1159\/000345367","article-title":"Dental caries from a molecular microbiological perspective","volume":"47","author":"B Nyvad","year":"2013","journal-title":"Caries Res"},{"issue":"7","key":"ref27","doi-asserted-by":"crossref","first-page":"e2836","DOI":"10.1371\/journal.pone.0002836","article-title":"Comparative analysis of human gut microbiota by barcoded pyrosequencing","volume":"3","author":"AF Andersson","year":"2008","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/nar\/gkn879","article-title":"The Ribosomal Database Project: improved alignments and new tools for rRNA analysis","volume":"37","author":"JR Cole","year":"2009","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"ref29","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1261\/rna.033266.112","article-title":"The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy","volume":"18","author":"A Ben\u00edtez-P\u00e1ez","year":"2012","journal-title":"RNA"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"R42","DOI":"10.1186\/gb-2012-13-6-r42","article-title":"Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples","volume":"13","author":"N Segata","year":"2012","journal-title":"Genome Biol"},{"issue":"23","key":"ref31","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"PD Schloss","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1186\/1471-2105-7-371","article-title":"UniFrac\u2014an online tool for comparing microbial community diversity in a phylogenetic context","volume":"7","author":"C Lozupone","year":"2006","journal-title":"BMC Bioinformatics"},{"issue":"4","key":"ref33","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.syapm.2008.07.001","article-title":"The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains","volume":"31","author":"P Yarza","year":"2008","journal-title":"Syst Appl Microbiol"},{"issue":"7402","key":"ref34","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nature11234","article-title":"Structure, function and diversity of the healthy human microbiome","volume":"486","year":"2012","journal-title":"Nature"},{"issue":"11","key":"ref35","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the normal bacterial flora of the oral cavity","volume":"43","author":"JA Aas","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref36","author":"M Wilson","year":"2005"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1007\/s00248-011-9989-2","article-title":"Adherence to streptococci facilitates Fusobacterium nucleatum integration into an oral microbial community","volume":"63","author":"X He","year":"2012","journal-title":"Microb Ecol"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1600-0528.1997.tb00904.x","article-title":"Caries prediction\u2014state of the art","volume":"25","author":"H Hausen","year":"1997","journal-title":"Community Dent Oral Epidemiol"},{"issue":"2","key":"ref39","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jebdp.2013.04.002","article-title":"Caries risk profiles using cariogram are predictive of caries incidence in permanent dentition","volume":"13","author":"M Tellez","year":"2013","journal-title":"J Evid Based Dent Pract"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1177\/00220345900690050801","article-title":"Distribution and prevalence of mutans streptococci in the human dentition","volume":"69","author":"B Lindquist","year":"1990","journal-title":"J Dent Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0198021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T01:24:31Z","timestamp":1604366671000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0198021"}},"subtitle":[],"editor":[{"given":"Ramy K.","family":"Aziz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,6,1]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2018,6,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0198021","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,1]]}}}