{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:31:19Z","timestamp":1790418679029,"version":"4.1.0"},"reference-count":82,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T00:00:00Z","timestamp":1529971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0199641","type":"journal-article","created":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T17:26:28Z","timestamp":1530033988000},"page":"e0199641","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genomic and phenotypic characterization of Borrelia afzelii BO23 and Borrelia garinii CIP 103362"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1834-5416","authenticated-orcid":true,"given":"S\u00e9bastien","family":"Bontemps-Gallo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Lawrence","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal L.","family":"Richards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1063-7131","authenticated-orcid":true,"given":"Frank C.","family":"Gherardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,6,26]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1099\/ijsem.0.001815","article-title":"Division of the genus Borrelia into two genera (corresponding to Lyme disease and relapsing fever groups) reflects their genetic and phenotypic distinctiveness and will lead to a better understanding of these two groups of microbes (Margos et al. (2016) There is inadequate evidence to support the division of the genus Borrelia. Int. J. Syst. Evol. Microbiol. doi: 10.1099\/ijsem.0.001717)","volume":"67","author":"AG Barbour","year":"2017","journal-title":"Int J Syst Evol Microbiol"},{"issue":"9814","key":"ref2","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0140-6736(11)60103-7","article-title":"Lyme borreliosis","volume":"379","author":"G Stanek","year":"2012","journal-title":"Lancet"},{"issue":"13","key":"ref3","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1056\/NEJM198303313081301","article-title":"The spirochetal etiology of Lyme disease","volume":"308","author":"AC Steere","year":"1983","journal-title":"N Engl J Med"},{"key":"ref4","article-title":"Physiologic and Genetic Factors Influencing the Zoonotic Cycle of Borrelia burgdorferi","author":"PE Stewart","year":"2017","journal-title":"Curr Top Microbiol Immunol"},{"issue":"4552","key":"ref5","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1126\/science.7043737","article-title":"Lyme disease-a tick-borne spirochetosis?","volume":"216","author":"W Burgdorfer","year":"1982","journal-title":"Science"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1001\/jama.2013.278331","article-title":"CDC estimates 300,000 US cases of Lyme disease annually","volume":"310","author":"BM Kuehn","year":"2013","journal-title":"JAMA"},{"issue":"10","key":"ref7","first-page":"1","article-title":"Surveillance for Lyme disease\u2014United States, 1992\u20132006","volume":"57","author":"RM Bacon","year":"2008","journal-title":"MMWR Surveill Summ"},{"key":"ref8","article-title":"Lyme borreliosis in Europe: Influences of Climate and Climate change, Epidemiology, Ecology and Adaptation Measures","author":"E Lindgren","year":"2006"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s10393-004-0139-x","article-title":"Effect of Climate Change on Lyme Disease Risk in North America","volume":"2","author":"JS Brownstein","year":"2005","journal-title":"Ecohealth"},{"issue":"4","key":"ref10","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"AG Barbour","year":"1984","journal-title":"Yale J Biol Med"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF01566969","article-title":"Isolation of a cultivable spirochete fromIxodes ricinus ticks of Switzerland","volume":"8","author":"AG Barbour","year":"1983","journal-title":"Current Microbiology"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1099\/00207713-42-3-378","article-title":"Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis","volume":"42","author":"G Baranton","year":"1992","journal-title":"Int J Syst Bacteriol"},{"issue":"5","key":"ref13","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/S1473-3099(15)00464-8","article-title":"Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study","volume":"16","author":"BS Pritt","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"ref14","article-title":"Centre National de r\u00e9f\u00e9rence des Borrelia\u2014Rapport annuel d\u2019activit\u00e9","author":"B Jaulhac","year":"2013"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"818","DOI":"10.3201\/eid2205.151806","article-title":"Differences in Genotype, Clinical Features, and Inflammatory Potential of Borrelia burgdorferi sensu stricto Strains from Europe and the United States","volume":"22","author":"T Cerar","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"16090","DOI":"10.1038\/nrdp.2016.90","article-title":"Lyme borreliosis","volume":"2","author":"AC Steere","year":"2016","journal-title":"Nat Rev Dis Primers"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1046\/j.1365-2958.2000.01698.x","article-title":"A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi","volume":"35","author":"S Casjens","year":"2000","journal-title":"Mol Microbiol"},{"issue":"6660","key":"ref18","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/37551","article-title":"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi","volume":"390","author":"CM Fraser","year":"1997","journal-title":"Nature"},{"issue":"24","key":"ref19","doi-asserted-by":"crossref","first-page":"6995","DOI":"10.1128\/JB.05951-11","article-title":"Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates","volume":"193","author":"SR Casjens","year":"2011","journal-title":"J Bacteriol"},{"issue":"3","key":"ref20","doi-asserted-by":"crossref","first-page":"e33280","DOI":"10.1371\/journal.pone.0033280","article-title":"Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmids","volume":"7","author":"SR Casjens","year":"2012","journal-title":"PLoS One"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1128\/JB.01158-10","article-title":"Whole-genome sequences of thirteen isolates of Borrelia burgdorferi","volume":"193","author":"SE Schutzer","year":"2011","journal-title":"J Bacteriol"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/JB.06263-11","article-title":"Whole-genome sequences of Borrelia bissettii, Borrelia valaisiana, and Borrelia spielmanii","volume":"194","author":"SE Schutzer","year":"2012","journal-title":"J Bacteriol"},{"key":"ref23","first-page":"103","article-title":"Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"FC Gherardini","year":"2010"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/nrmicro2714","article-title":"Of ticks, mice and men: understanding the dual-host lifestyle of Lyme disease spirochaetes","volume":"10","author":"JD Radolf","year":"2012","journal-title":"Nat Rev Microbiol"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1684\/ecn.2017.0396","article-title":"The role of host immune cells and Borrelia burgdorferi antigens in the etiology of Lyme disease","volume":"28","author":"D Verhaegh","year":"2017","journal-title":"Eur Cytokine Netw"},{"key":"ref26","first-page":"361","article-title":"Borrelia burgdorferi: Methods and Protocols","author":"U Pal","year":"2018"},{"issue":"10","key":"ref27","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1128\/JCM.33.10.2679-2685.1995","article-title":"Analysis and comparison of plasmid profiles of Borrelia burgdorferi sensu lato strains","volume":"33","author":"Y Xu","year":"1995","journal-title":"J Clin Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"56","DOI":"10.3389\/fcimb.2014.00056","article-title":"The cyclic-di-GMP signaling pathway in the Lyme disease spirochete, Borrelia burgdorferi","volume":"4","author":"EA Novak","year":"2014","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/j.1365-2958.2010.07191.x","article-title":"Analysis of a Borrelia burgdorferi phosphodiesterase demonstrates a role for cyclic-di-guanosine monophosphate in motility and virulence","volume":"77","author":"SZ Sultan","year":"2010","journal-title":"Mol Microbiol"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1186\/s12864-018-4597-x","article-title":"Primordial origin and diversification of plasmids in Lyme disease agent bacteria","volume":"19","author":"SR Casjens","year":"2018","journal-title":"BMC Genomics"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1186\/s12864-017-3553-5","article-title":"Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi","volume":"18","author":"SR Casjens","year":"2017","journal-title":"BMC Genomics"},{"issue":"24","key":"ref32","doi-asserted-by":"crossref","first-page":"7014","DOI":"10.1128\/JB.01863-12","article-title":"Genome sequence of Borrelia afzelii Strain HLJ01, isolated from a patient in China","volume":"194","author":"BG Jiang","year":"2012","journal-title":"J Bacteriol"},{"issue":"6","key":"ref33","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01315-14","article-title":"Complete Genome Sequencing of Borrelia valaisiana and Borrelia afzelii Isolated from Ixodes persulcatus Ticks in Western Siberia","volume":"2","author":"AM Kurilshikov","year":"2014","journal-title":"Genome Announc"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"e0120548","DOI":"10.1371\/journal.pone.0120548","article-title":"Complete genome sequence of Borrelia afzelii K78 and comparative genome analysis","volume":"10","author":"W Schuler","year":"2015","journal-title":"PLoS One"},{"issue":"20","key":"ref35","doi-asserted-by":"crossref","first-page":"5713","DOI":"10.1128\/JB.01360-12","article-title":"Whole-genome sequencing of Borrelia garinii BgVir, isolated from Taiga ticks (Ixodes persulcatus)","volume":"194","author":"EV Brenner","year":"2012","journal-title":"J Bacteriol"},{"issue":"23","key":"ref36","doi-asserted-by":"crossref","first-page":"6660","DOI":"10.1128\/JB.01844-12","article-title":"Genome sequence of Borrelia garinii strain NMJW1, isolated from China","volume":"194","author":"B Jiang","year":"2012","journal-title":"J Bacteriol"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1046\/j.1365-2958.2001.02256.x","article-title":"Isolation of a circular plasmid region sufficient for autonomous replication and transformation of infectious Borrelia burgdorferi","volume":"39","author":"PE Stewart","year":"2001","journal-title":"Mol Microbiol"},{"issue":"5","key":"ref38","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/JB.01176-10","article-title":"Defining the plasmid-borne restriction-modification systems of the Lyme disease spirochete Borrelia burgdorferi","volume":"193","author":"RO Rego","year":"2011","journal-title":"J Bacteriol"},{"issue":"9","key":"ref39","doi-asserted-by":"crossref","first-page":"4798","DOI":"10.1128\/IAI.70.9.4798-4804.2002","article-title":"Decreased electroporation efficiency in Borrelia burgdorferi containing linear plasmids lp25 and lp56: impact on transformation of infectious B. burgdorferi","volume":"70","author":"MB Lawrenz","year":"2002","journal-title":"Infect Immun"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/nar\/gkt990","article-title":"Highlights of the DNA cutters: a short history of the restriction enzymes","volume":"42","author":"WA Loenen","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"ref41","doi-asserted-by":"crossref","first-page":"e1005791","DOI":"10.1371\/journal.ppat.1005791","article-title":"Two Different Virulence-Related Regulatory Pathways in Borrelia burgdorferi Are Directly Affected by Osmotic Fluxes in the Blood Meal of Feeding Ixodes Ticks","volume":"12","author":"S Bontemps-Gallo","year":"2016","journal-title":"PLoS Pathog"},{"issue":"10","key":"ref42","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1007\/s001340051011","article-title":"Assessment of regional tissue oxygenation","volume":"25","author":"M Siegemund","year":"1999","journal-title":"Intensive Care Med"},{"issue":"5","key":"ref43","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1007\/s001340051209","article-title":"Subcutaneous oxygen tensions provide similar information to ileal luminal CO2 tensions in an animal model of haemorrhagic shock","volume":"26","author":"B Venkatesh","year":"2000","journal-title":"Intensive Care Med"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.mib.2015.09.006","article-title":"Spirochetal motility and chemotaxis in the natural enzootic cycle and development of Lyme disease","volume":"28","author":"MA Motaleb","year":"2015","journal-title":"Curr Opin Microbiol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1146\/annurev.genet.36.041602.134359","article-title":"Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes","volume":"36","author":"NW Charon","year":"2002","journal-title":"Annu Rev Genet"},{"issue":"6","key":"ref46","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1128\/IAI.01228-12","article-title":"Motility is crucial for the infectious life cycle of Borrelia burgdorferi","volume":"81","author":"SZ Sultan","year":"2013","journal-title":"Infect Immun"},{"issue":"20","key":"ref47","doi-asserted-by":"crossref","first-page":"10899","DOI":"10.1073\/pnas.200221797","article-title":"Borrelia burgdorferi periplasmic flagella have both skeletal and motility functions","volume":"97","author":"MA Motaleb","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref48","first-page":"122","article-title":"Biology of Ticks","volume":"1","author":"DE Sonenshine","year":"2014"},{"issue":"3","key":"ref49","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1111\/j.1365-2958.2008.06204.x","article-title":"Borrelia burgdorferi membranes are the primary targets of reactive oxygen species","volume":"68","author":"JA Boylan","year":"2008","journal-title":"Mol Microbiol"},{"issue":"1\u20132","key":"ref50","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1574-6968.1994.tb07163.x","article-title":"Simultaneous measurement by flow cytometry of phagocytosis and metabolic burst induced in phagocytic cells in whole blood by Borrelia burgdorferi","volume":"122","author":"M Cinco","year":"1994","journal-title":"FEMS Microbiol Lett"},{"issue":"1","key":"ref51","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1093\/infdis\/163.1.150","article-title":"Infectivity of Borrelia burgdorferi correlates with resistance to elimination by phagocytic cells","volume":"163","author":"K Georgilis","year":"1991","journal-title":"J Infect Dis"},{"issue":"2","key":"ref52","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1128\/IAI.46.2.608-611.1984","article-title":"Human phagocyte interactions with the Lyme disease spirochete","volume":"46","author":"PK Peterson","year":"1984","journal-title":"Infect Immun"},{"issue":"1","key":"ref53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1988.tb31832.x","article-title":"IntroductionBorrelia burgdorferi Infection: A Neurologist\u2019s Perspective","volume":"539","author":"L Reik","year":"1988","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"1","key":"ref54","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1086\/315195","article-title":"Borrelia burgdorferi\u2014induced oxidative burst, calcium mobilization, and phagocytosis of human neutrophils are complement dependent","volume":"181","author":"J Suhonen","year":"2000","journal-title":"J Infect Dis"},{"issue":"10","key":"ref55","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1016\/j.jinsphys.2010.02.014","article-title":"Meeting the challenges of on-host and off-host water balance in blood-feeding arthropods","volume":"56","author":"JB Benoit","year":"2010","journal-title":"J Insect Physiol"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"21047","DOI":"10.1038\/srep21047","article-title":"Multiple functions of Na\/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis","volume":"6","author":"D Kim","year":"2016","journal-title":"Sci Rep"},{"issue":"1\u20132","key":"ref57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182000013093","article-title":"The water balance in Ixodes ricinus L. and certain other species of ticks","volume":"37","author":"AD Lees","year":"1946","journal-title":"Parasitology"},{"issue":"3\u20134","key":"ref58","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1242\/jeb.23.3-4.379","article-title":"Transpiration and the Structure of the Epicuticle in Ticks","volume":"23","author":"AD Lees","year":"1947","journal-title":"Journal of Experimental Biology"},{"issue":"2","key":"ref59","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1242\/jeb.25.2.145","article-title":"The Sensory Physiology of the Sheep Tick, Ixodes Ricinus L","volume":"25","author":"AD Lees","year":"1948","journal-title":"Journal of Experimental Biology"},{"issue":"2","key":"ref60","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1242\/jeb.58.2.537","article-title":"Ion and Water Balance in the Ixodid Tick Dermacentor Andersoni","volume":"58","author":"WR Kaufman","year":"1973","journal-title":"Journal of Experimental Biology"},{"issue":"2","key":"ref61","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1242\/jeb.58.2.549","article-title":"Ion and Water Balance in the Ixodid Tick Dermacentor Andersoni","volume":"58","author":"WR Kaufman","year":"1973","journal-title":"Journal of Experimental Biology"},{"issue":"2","key":"ref62","first-page":"523","article-title":"Ion and Water Balance in the Ixodid Tick Dermacentor Andersoni","volume":"58","author":"WR Kaufman","year":"1973","journal-title":"I Routes of Ion and Water Excretion"},{"issue":"2","key":"ref63","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF02616113","article-title":"Osmolality of mammalian blood and of media for culture of mammalian cells","volume":"6","author":"C Waymouth","year":"1970","journal-title":"In Vitro"},{"issue":"7647","key":"ref64","doi-asserted-by":"crossref","first-page":"S54","DOI":"10.1038\/543S54a","article-title":"Climate change: As the ice melts","volume":"543","author":"E Dolgin","year":"2017","journal-title":"Nature"},{"issue":"5","key":"ref65","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.ttbdis.2015.05.005","article-title":"Climate change influences on the annual onset of Lyme disease in the United States","volume":"6","author":"AJ Monaghan","year":"2015","journal-title":"Ticks Tick Borne Dis"},{"issue":"7","key":"ref66","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1111\/eva.12165","article-title":"Climate change and habitat fragmentation drive the occurrence of Borrelia burgdorferi, the agent of Lyme disease, at the northeastern limit of its distribution","volume":"7","author":"JA Simon","year":"2014","journal-title":"Evol Appl"},{"issue":"9","key":"ref67","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1089\/vbz.2017.2127","article-title":"Brave New Worlds: The Expanding Universe of Lyme Disease","volume":"17","author":"BL Stone","year":"2017","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"6","key":"ref68","doi-asserted-by":"crossref","first-page":"e0157706","DOI":"10.1371\/journal.pone.0157706","article-title":"Comparison of Growth of Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi Sensu Stricto at Five Different Temperatures","volume":"11","author":"G Veinovic","year":"2016","journal-title":"PLoS One"},{"issue":"10","key":"ref69","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1099\/00222615-47-10-929","article-title":"Growth temperature ranges of Borrelia burgdorferi sensu lato strains","volume":"47","author":"Z Hubalek","year":"1998","journal-title":"J Med Microbiol"},{"issue":"1","key":"ref70","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1186\/s13071-017-2187-4","article-title":"The abundance of the Lyme disease pathogen Borrelia afzelii declines over time in the tick vector Ixodes ricinus","volume":"10","author":"M Jacquet","year":"2017","journal-title":"Parasit Vectors"},{"issue":"11","key":"ref71","doi-asserted-by":"crossref","first-page":"4145","DOI":"10.1128\/JCM.39.11.4145-4148.2001","article-title":"Use of quantitative PCR to measure density of Borrelia burgdorferi in the midgut and salivary glands of feeding tick vectors","volume":"39","author":"J Piesman","year":"2001","journal-title":"J Clin Microbiol"},{"issue":"357","key":"ref72","first-page":"pe43","article-title":"Osmosensing by bacteria","volume":"2006","author":"JM Wood","year":"2006","journal-title":"Sci STKE"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1146\/annurev-micro-090110-102815","article-title":"Bacterial osmoregulation: a paradigm for the study of cellular homeostasis","volume":"65","author":"JM Wood","year":"2011","journal-title":"Annu Rev Microbiol"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.3389\/fmicb.2016.01397","article-title":"The Nucleotide Excision Repair Pathway Protects Borrelia burgdorferi from Nitrosative Stress in Ixodes scapularis Ticks","volume":"7","author":"TJ Bourret","year":"2016","journal-title":"Front Microbiol"},{"issue":"2","key":"ref75","doi-asserted-by":"crossref","first-page":"e1006225","DOI":"10.1371\/journal.ppat.1006225","article-title":"A high-throughput genetic screen identifies previously uncharacterized Borrelia burgdorferi genes important for resistance against reactive oxygen and nitrogen species","volume":"13","author":"ME Ramsey","year":"2017","journal-title":"PLoS Pathog"},{"issue":"20","key":"ref76","doi-asserted-by":"crossref","first-page":"11684","DOI":"10.1073\/pnas.2032956100","article-title":"Borrelia oxidative stress response regulator, BosR: a distinctive Zn-dependent transcriptional activator","volume":"100","author":"JA Boylan","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"9","key":"ref77","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1128\/IAI.64.9.3870-3876.1996","article-title":"Correlation of plasmids with infectivity of Borrelia burgdorferi sensu stricto type strain B31","volume":"64","author":"Y Xu","year":"1996","journal-title":"Infect Immun"},{"issue":"4","key":"ref78","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1128\/IAI.70.4.2139-2150.2002","article-title":"Clonal polymorphism of Borrelia burgdorferi strain B31 MI: implications for mutagenesis in an infectious strain background","volume":"70","author":"AF Elias","year":"2002","journal-title":"Infect Immun"},{"issue":"2","key":"ref79","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0882-4010(90)90075-2","article-title":"Analysis of supercoiled circular plasmids in infectious and non-infectious Borrelia burgdorferi","volume":"8","author":"WJ Simpson","year":"1990","journal-title":"Microb Pathog"},{"issue":"2","key":"ref80","first-page":"e00404","article-title":"Function of the Borrelia burgdorferi FtsH Homolog Is Essential for Viability both In Vitro and In Vivo and Independent of HflK\/C","volume":"7","author":"CY Chu","year":"2016","journal-title":"MBio"},{"key":"ref81","first-page":"253","article-title":"Electrotransformation of the spirochete Borrelia burgdorferi","volume":"47","author":"DS Samuels","year":"1995","journal-title":"Methods Mol Biol"},{"issue":"4","key":"ref82","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1128\/JCM.41.4.1791-1793.2003","article-title":"In vitro susceptibility testing of four antibiotics against Borrelia burgdorferi: a comparison of results for the three genospecies Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto","volume":"41","author":"M Sicklinger","year":"2003","journal-title":"J Clin Microbiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0199641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T12:27:12Z","timestamp":1661516832000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0199641"}},"subtitle":[],"editor":[{"given":"Brian","family":"Stevenson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,6,26]]},"references-count":82,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2018,6,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0199641","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,26]]}}}