{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:44:34Z","timestamp":1772203474491,"version":"3.50.1"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T00:00:00Z","timestamp":1538524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grant-in-aid for Scientific Research (C)","award":["18K11056"],"award-info":[{"award-number":["18K11056"]}]},{"name":"Grant-in-aid for Scientific Research Young Scientists (B)","award":["16K18924"],"award-info":[{"award-number":["16K18924"]}]},{"name":"Grant-in-aid for Scientific Research (C)","award":["17K00900"],"award-info":[{"award-number":["17K00900"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0199856","type":"journal-article","created":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T13:33:37Z","timestamp":1538573617000},"page":"e0199856","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Eldecalcitol is more effective in promoting osteogenesis than alfacalcidol in Cyp27b1-knockout mice"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6462-9479","authenticated-orcid":true,"given":"Yoshihisa","family":"Hirota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimie","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keigo","family":"Isomoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Sakaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Kubodera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya","family":"Kamao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Osakabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitomo","family":"Suhara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Okano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,10,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.1073\/pnas.72.6.2076","article-title":"Group-specific component (Gc) proteins bind vitamin D and 25-hydroxyvitamin D","volume":"72","author":"SP Daiger","year":"1975","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"3294","DOI":"10.1073\/pnas.85.10.3294","article-title":"Cloning and expression of full-length cDNA encoding human vitamin D receptor","volume":"85","author":"AR Baker","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/355446a0","article-title":"Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D<sub>3<\/sub> signalling","volume":"355","author":"SA Kliewer","year":"1992","journal-title":"Nature"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0378-1119(00)00052-4","article-title":"Mechanisms of gene regulation by vitamin D<sub>3<\/sub> receptor: a network of coactivator interactions","volume":"246","author":"C Rachez","year":"2000","journal-title":"Gene"},{"key":"ref5","first-page":"89","article-title":"Vitamin D-regulated gene expression","volume":"3","author":"H Darwish","year":"1993","journal-title":"Crit. Rev. Eukaryot. Gene Exp"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.3945\/ajcn.116.145110","article-title":"Dietary protein and bone health: a systematic review and meta-analysis from the National Osteoporosis Foundation","volume":"105","author":"MM Shams-White","year":"2017","journal-title":"Am J Clin Nutr"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1038\/238271a0","article-title":"Effect of calcitonin on vitamin D metabolism","volume":"238","author":"L Galante","year":"1972","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1126\/science.277.5333.1827","article-title":"25-Hydroxyvitamin D<sub>3<\/sub> 1alpha-hydroxylase and vitamin D synthesis","volume":"277","author":"K Takeyama","year":"1997","journal-title":"Science"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s00223001118","article-title":"Bone mass and markers of bone and calcium metabolism in postmenopausal women treated with 1,25-dihydroxyvitamin D (Calcitriol) for four years","volume":"67","author":"S Sairanen","year":"2000","journal-title":"Calcif Tissue Int"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"4410","DOI":"10.1248\/cpb.34.4410","article-title":"Synthetic studies of vitamin D<sub>3<\/sub> analogues. VIII. Synthesis of 22-oxavitamin D<sub>3<\/sub> analogues","volume":"34","author":"E Murayama","year":"1986","journal-title":"Chem Pharm Bull"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/BF02378983","article-title":"Decrease of vertebral fracture in osteoporotics by administration of 1\u03b1-hydroxy-vitamin D<sub>3<\/sub>","volume":"10","author":"Y. Hayashi","year":"1992","journal-title":"J Bone Mineral Metab"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1007\/BF00305521","article-title":"Effects of 1 alpha-hydroxyvitamin D<sub>3<\/sub> on lumbar bone mineral density and vertebral fractures in patients with postmenopausal osteoporosis","volume":"54","author":"H Orimo","year":"1994","journal-title":"Calcif Tissue Int"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00223-001-2090-y","article-title":"A comparison of alfacalcidol and menatetrenone for the treatment of bone loss in an ovariectomized rat model of osteoporosis","volume":"71","author":"A Shiraishi","year":"2002","journal-title":"Calcif Tissue Int"},{"key":"ref14","first-page":"1143","article-title":"Comparison of the inhibitions of proliferation of normal and psoriatic fibroblasts by 1 alpha,25-dihydroxyvitamin D<sub>3<\/sub> and synthetic analogues of vitamin D<sub>3<\/sub> with an oxygen atom in their side chain","volume":"19","author":"S Morimoto","year":"1989","journal-title":"Biochem Int"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e00120","DOI":"10.1002\/prp2.120","article-title":"Human hepatic metabolism of the anti-osteoporosis drug eldecalcitol involves sterol C4-methyl oxidase","volume":"3","author":"K Yasuda","year":"2015","journal-title":"Pharmacol Res Perspect"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1248\/cpb.41.1111","article-title":"Synthetic studies of vitamin D analogues. XIV. Synthesis and calcium regulating activity of vitamin D<sub>3<\/sub> analogues bearing a hydroxyalkoxy group at the 2 beta-position","volume":"41","author":"K Miyamoto","year":"1993","journal-title":"Chem Pharm Bull"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1016\/0006-291X(89)91140-6","article-title":"-1 alpha, 25-dihydroxyvitamin D<sub>3<\/sub>, a novel synthetic vitamin D<sub>3<\/sub> derivative, on calcium metabolism","volume":"163","author":"T Okano","year":"1989","journal-title":"Biochem Biophys Res Commun"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1530\/JOE-15-0332","article-title":"Eldecalcitol prevents endothelial dysfunction in postmenopausal osteoporosis model rats","volume":"228","author":"K Serizawa","year":"2016","journal-title":"J Endocrinol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"8050","DOI":"10.1016\/j.bmc.2006.07.039","article-title":"Synthesis and evaluation of a 3-position diastereomer of 1\u03b1,25-dihydroxy-2\u03b2-(3-hydroxypropoxy)vitamin D3 (ED-71)","volume":"14","author":"S Hatakeyama","year":"2006","journal-title":"Bioorg Med Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1007\/BF00296065","article-title":"A novel synthetic vitamin D analogue, 2\u03b2-(3-hydroxypropoxy)1\u03b1, 25-dihydroxyvitamin D3 (ED-71), increases bone mass by stimulating the bone formation in normal and ovariectomized rats","volume":"54","author":"H Tsurukami","year":"1994","journal-title":"Calcif Tissue Int"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/jcb.10346","article-title":"A new active vitamin D analog, ED-71, causes increase in bone mass with preferential effects on bone in osteoporotic patients","volume":"88","author":"N Kubodera","year":"2003","journal-title":"J Cell Biochem"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.1210\/jc.2004-2552","article-title":"A new active vitamin D, ED-71, increases bone mass in osteoporotic patients under vitamin D supplementation: a randomized, double-blind, placebo-controlled clinical trial","volume":"90","author":"T Matsumoto","year":"2005","journal-title":"J Clin Endocrinol Metab"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3135","DOI":"10.1210\/endo.142.7.8281","article-title":"Targeted inactivation of the 25-hydroxyvitamin D3-1\u03b1-hydroxylase gene (CYP27B1) creates an animal model of pseudovitamin D-deficiency rickets","volume":"142","author":"O Dardenne","year":"2001","journal-title":"Endocrinology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"7498","DOI":"10.1073\/pnas.131029498","article-title":"Targeted ablation of the 25-hydroxyvitamin D 1\u03b1-hydroxylase enzyme: evidence for skeletal, reproductive, and immune dysfunction","volume":"98","author":"DK Panda","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1073\/pnas.0509734103","article-title":"CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D<sub>3<\/sub>-1\u03b1-hydroxylase promoter activity in the skin","volume":"103","author":"JL Vanhooke","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.bbrc.2016.12.139","article-title":"Nongenomic effects of 1\u03b1,25-dihydroxyvitamin D3 on cartilage formation deduced from comparisons between Cyp27b1 and Vdr knockout mice","volume":"483","author":"Y Hirota","year":"2017","journal-title":"Biochem Biophys Res Commun"},{"key":"ref27","first-page":"327","article-title":"Rescue of the pseudo-vitamin D deficiency rickets phenotype of CYP27B1-deficient mice by treatment With 1,25-dihydroxyvitamin D<sub>3<\/sub>: biochemical, histomorphometric, and biomechanical analyses","volume":"89\u201390","author":"O Dardenne","year":"2004","journal-title":"J Steroid Biochem Mol Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"2984","DOI":"10.1073\/pnas.90.7.2984","article-title":"Identification of sequence elements in mouse calbindin-D28k gene that confer 1,25-dihydroxyvitamin D<sub>3<\/sub>- and butyrate-inducible responses","volume":"90","author":"RK Gill","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1210\/endo.141.7.7557","article-title":"Transgenic analysis of the response of the rat calbindin-D <sub>9k<\/sub> gene to vitamin D","volume":"141","author":"S Colnot","year":"2000","journal-title":"Endocrinology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1194\/jlr.M700293-JLR200","article-title":"Lithocholic acid derivatives act as selective vitamin D receptor modulators without inducing hypercalcemia","volume":"49","author":"M Ishizawa","year":"2008","journal-title":"J Lipid Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1021\/bi00052a011","article-title":"Structural characterization of the gene encoding rat 25-hydroxyvitamin D<sub>3<\/sub> 24-hydroxylase","volume":"32","author":"Y Ohyama","year":"1993","journal-title":"Biochemistry"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.2165\/11206790-000000000-00000","article-title":"Eldecalcitol: a review of its use in the treatment of osteoporosis","volume":"71","author":"M Sanford","year":"2011","journal-title":"Drugs"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1124\/dmd.104.003038","article-title":"Metabolism of 2\u03b1 -propoxy-1\u03b1,25-dihydroxyvitamin D3 and 2\u03b1-(3-hydroxypropoxy)-1 \u03b1,25-dihydroxyvitamin D3 by human CYP27A1 and CYP24A1","volume":"33","author":"D Abe","year":"2005","journal-title":"Drug Metab Dispos"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/jbmr.5650110105","article-title":"1\u03b1,25-dihydroxyvitamin D<sub>3<\/sub> inhibits cell growth and chondrogenesis of a clonal mouse EC cell line, ATDC5","volume":"11","author":"H Akiyama","year":"1996","journal-title":"J Bone Miner Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.bone.2006.08.017","article-title":"ED-71, a novel vitamin D analog, promotes bone formation and angiogenesis and inhibits bone resorption after bone marrow ablation","volume":"40","author":"N Okuda","year":"2007","journal-title":"Bone"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/jbmr.555","article-title":"Daily administration of eldecalcitol (ED-71), an active vitamin D analog, increases bone mineral density by suppressing RANKL expression in mouse trabecular bone","volume":"27","author":"S Harada","year":"2012","journal-title":"J Bone Miner Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/S8756-3282(02)00682-8","article-title":"ED-71, a vitamin D analog, is a more potent inhibitor of bone resorption than alfacalcidol in an estrogen-deficient rat model of osteoporosis","volume":"30","author":"Y Uchiyama","year":"2002","journal-title":"Bone"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/S8756-3282(02)00735-4","article-title":"Misconceptions (2): turnover is always higher in cancellous than in cortical bone","volume":"30","author":"AM Parfitt","year":"2002","journal-title":"Bone"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1172\/JCI24742","article-title":"c-Fos protein as a target of anti-osteoclastogenic action of vitamin D, and synthesis of new analogs","volume":"116","author":"H Takasu","year":"2006","journal-title":"J Clin Invest"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/0006-291X(92)92336-V","article-title":"The effect of active vitamin D<sub>3<\/sub> analogs and dexamethasone on the expression of osteocalcin gene in rat tibiae in vivo","volume":"189","author":"T Ikeda","year":"1992","journal-title":"Biochem Biophys Res Commun"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.jsbmb.2012.10.004","article-title":"Eldecalcitol and calcitriol stimulates &apos;bone minimodeling,&apos; focal bone formation without prior bone resorption, in rat trabecular bone","volume":"136","author":"H Saito","year":"2013","journal-title":"J Steroid Biochem Mol Biol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1359\/jbmr.1998.13.5.763","article-title":"A unitary model for involutional osteoporosis: estrogen deficiency causes both type I and type II osteoporosis in postmenopausal women and contributes to bone loss in aging men","volume":"13","author":"BL Riggs","year":"1998","journal-title":"J Bone Miner Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0199856","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T12:10:42Z","timestamp":1693915842000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0199856"}},"subtitle":[],"editor":[{"given":"Makoto","family":"Makishima","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,10,3]]},"references-count":42,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0199856","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/349837","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,3]]}}}