{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:45:27Z","timestamp":1792694727891,"version":"4.5.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["Contract No. HHSN272201100016I; Task Order HHSN27200005"],"award-info":[{"award-number":["Contract No. HHSN272201100016I; Task Order HHSN27200005"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["Contract No. HHSN272201100013I; Task Order HHSN27200006"],"award-info":[{"award-number":["Contract No. HHSN272201100013I; Task Order HHSN27200006"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0200043","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T13:54:27Z","timestamp":1539784467000},"page":"e0200043","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["In vitro toxicity and efficacy of verdinexor, an exportin 1 inhibitor, on opportunistic viruses affecting immunocompromised individuals"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3585-6373","authenticated-orcid":true,"given":"Douglas G.","family":"Widman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savanna","family":"Gornisiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Shacham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Tamir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,10,17]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/nrc1274","article-title":"Nuclear transport and cancer: from mechanism to intervention","volume":"4","author":"TR Kau","year":"2004","journal-title":"Nat Rev Cancer"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/0092-8674(95)90436-0","article-title":"The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs","volume":"82","author":"U Fischer","year":"1995","journal-title":"Cell"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/wrna.101","article-title":"mRNA export and cancer","volume":"3","author":"N Siddiqui","year":"2012","journal-title":"Wiley Interdiscip Rev RNA"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/S0092-8674(00)80371-2","article-title":"CRM1 is an export receptor for leucine-rich nuclear export signals","volume":"90","author":"M Fornerod","year":"1997","journal-title":"Cell"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.3389\/fmicb.2017.01171","article-title":"CRM1 Inhibitors for Antiviral Therapy","volume":"8","author":"C Mathew","year":"2017","journal-title":"Front Microbiol"},{"issue":"17","key":"ref6","doi-asserted-by":"crossref","first-page":"10228","DOI":"10.1128\/JVI.01774-14","article-title":"Verdinexor, a novel selective inhibitor of nuclear export, reduces influenza a virus replication in vitro and in vivo","volume":"88","author":"O Perwitasari","year":"2014","journal-title":"J Virol"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/j.ebiom.2015.07.041","article-title":"Human Exportin-1 is a Target for Combined Therapy of HIV and AIDS Related Lymphoma","volume":"2","author":"E Boons","year":"2015","journal-title":"EBioMedicine"},{"issue":"3\u20134","key":"ref8","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0166-0934(86)90030-3","article-title":"Rapid method for the identification and screening of herpesviruses by DNA fingerprinting combined with blot hybridization","volume":"14","author":"SI Chowdhury","year":"1986","journal-title":"J Virol Methods"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"36226","DOI":"10.1038\/srep36226","article-title":"The nucleolar protein GLTSCR2 is required for efficient viral replication","volume":"6","author":"P Wang","year":"2016","journal-title":"Sci Rep"},{"issue":"11","key":"ref10","doi-asserted-by":"crossref","first-page":"e0167221","DOI":"10.1371\/journal.pone.0167221","article-title":"Antiviral Efficacy of Verdinexor In Vivo in Two Animal Models of Influenza A Virus Infection","volume":"11","author":"O Perwitasari","year":"2016","journal-title":"PLoS One"},{"issue":"4","key":"ref11","first-page":"325","article-title":"Culture conditions required for primary isolation and study of mouse blood monocytes","volume":"31","author":"JJ Wirth","year":"1982","journal-title":"J Reticuloendothel Soc"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1128\/JCM.22.4.656-658.1985","article-title":"Rapid detection of herpes simplex virus DNA in human brain tissue by in situ hybridization","volume":"22","author":"B Forghani","year":"1985","journal-title":"J Clin Microbiol"},{"issue":"1\u20132","key":"ref13","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0166-0934(85)90012-6","article-title":"In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infections","volume":"12","author":"SA Gomes","year":"1985","journal-title":"J Virol Methods"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0003-2697(83)90145-8","article-title":"A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution","volume":"131","author":"FG Nobrega","year":"1983","journal-title":"Anal Biochem"},{"issue":"16","key":"ref15","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1056\/NEJM198304213081603","article-title":"Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization","volume":"308","author":"S Chou","year":"1983","journal-title":"N Engl J Med"},{"issue":"1\u20132","key":"ref16","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jviromet.2007.04.001","article-title":"A rapid DNA hybridization assay for the evaluation of antiviral compounds against Epstein-Barr virus","volume":"144","author":"MN Prichard","year":"2007","journal-title":"J Virol Methods"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/S0076-6879(80)65051-4","article-title":"Rapid DNA isolations for enzymatic and hybridization analysis","volume":"65","author":"RW Davis","year":"1980","journal-title":"Methods Enzymol"},{"issue":"6","key":"ref18","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1182\/blood.V98.6.1971","article-title":"Quantification of polyoma BK viruria in hemorrhagic cystitis complicating bone marrow transplantation","volume":"98","author":"AY Leung","year":"2001","journal-title":"Blood"},{"issue":"20","key":"ref19","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1016\/j.bmcl.2016.08.080","article-title":"Dihydropyrimidinones and -thiones with improved activity against human polyomavirus family members","volume":"26","author":"A Manos-Turvey","year":"2016","journal-title":"Bioorg Med Chem Lett"},{"issue":"19","key":"ref20","doi-asserted-by":"crossref","first-page":"10213","DOI":"10.1128\/JVI.77.19.10213-10226.2003","article-title":"mRNA splicing regulates human papillomavirus type 11 E1 protein production and DNA replication","volume":"77","author":"W Deng","year":"2003","journal-title":"J Virol"},{"issue":"11","key":"ref21","doi-asserted-by":"crossref","first-page":"e0005122","DOI":"10.1371\/journal.pntd.0005122","article-title":"Selective Inhibitor of Nuclear Export (SINE) Compounds Alter New World Alphavirus Capsid Localization and Reduce Viral Replication in Mammalian Cells","volume":"10","author":"L Lundberg","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"ref22","unstructured":"Tamir S, Perwitasari O, Johnson S, Yan X, Drolen C, Shacham S, et al. Verdinexor, a Clinical-Stage Selective Inhibitor of Nuclear Export (SINE) Compound, Demonstrates a Wide Therapeutic Window and Low Susceptibility to Resistance Development in Mouse Models of Influenza A. ISIRV; August 26, 2016; Chicago, IL2016."},{"issue":"8","key":"ref23","doi-asserted-by":"crossref","first-page":"6872","DOI":"10.1128\/JVI.73.8.6872-6881.1999","article-title":"Association with the cellular export receptor CRM 1 mediates function and intracellular localization of Epstein-Barr virus SM protein, a regulator of gene expression","volume":"73","author":"SM Boyle","year":"1999","journal-title":"J Virol"},{"issue":"21","key":"ref24","doi-asserted-by":"crossref","first-page":"11730","DOI":"10.1128\/JVI.02760-06","article-title":"Nuclear export of the human cytomegalovirus tegument protein pp65 requires cyclin-dependent kinase activity and the Crm1 exporter","volume":"81","author":"V Sanchez","year":"2007","journal-title":"J Virol"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"5573","DOI":"10.1073\/pnas.082420599","article-title":"A Kaposi&apos;s sarcoma-associated herpesviral protein inhibits virus-mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation","volume":"99","author":"FX Zhu","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1128\/JVI.02209-08","article-title":"Identification of the nuclear export and adjacent nuclear localization signals for ORF45 of Kaposi&apos;s sarcoma-associated herpesvirus","volume":"83","author":"X Li","year":"2009","journal-title":"J Virol"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1128\/JVI.06275-11","article-title":"CRM1-dependent transport supports cytoplasmic accumulation of adenoviral early transcripts","volume":"86","author":"M Schmid","year":"2012","journal-title":"J Virol"},{"issue":"4","key":"ref28","first-page":"172","article-title":"BK virus infection in transplant recipients: clinical manifestations, treatment options and the immune response","volume":"70","author":"MC van Aalderen","year":"2012","journal-title":"Neth J Med"},{"issue":"10","key":"ref29","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1002\/jcp.25329","article-title":"Emerging From the Unknown: Structural and Functional Features of Agnoprotein of Polyomaviruses","volume":"231","author":"AS Saribas","year":"2016","journal-title":"J Cell Physiol"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.virol.2006.01.025","article-title":"Human polyomavirus JCV late leader peptide region contains important regulatory elements","volume":"349","author":"I Akan","year":"2006","journal-title":"Virology"},{"issue":"3","key":"ref31","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1440-1789.2007.00878.x","article-title":"An siRNA against JC virus (JCV) agnoprotein inhibits JCV infection in JCV-producing cells inoculated in nude mice","volume":"28","author":"T Matoba","year":"2008","journal-title":"Neuropathology"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"e1000801","DOI":"10.1371\/journal.ppat.1000801","article-title":"The human polyoma JC virus agnoprotein acts as a viroporin","volume":"6","author":"T Suzuki","year":"2010","journal-title":"PLoS Pathog"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/neup.12203","article-title":"Progressive multifocal leukoencephalopathy: Dot-shaped inclusions and virus-host interactions","volume":"35","author":"Y Shishido-Hara","year":"2015","journal-title":"Neuropathology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1186\/1743-422X-8-255","article-title":"Infection by agnoprotein-negative mutants of polyomavirus JC and SV40 results in the release of virions that are mostly deficient in DNA content","volume":"8","author":"IK Sariyer","year":"2011","journal-title":"Virol J"},{"issue":"36","key":"ref35","doi-asserted-by":"crossref","first-page":"5574","DOI":"10.1038\/sj.onc.1205744","article-title":"Evidence for dysregulation of cell cycle by human polyomavirus, JCV, late auxiliary protein","volume":"21","author":"A Darbinyan","year":"2002","journal-title":"Oncogene"},{"issue":"16","key":"ref36","doi-asserted-by":"crossref","first-page":"8593","DOI":"10.1128\/JVI.78.16.8593-8600.2004","article-title":"Role of JC virus agnoprotein in DNA repair","volume":"78","author":"A Darbinyan","year":"2004","journal-title":"J Virol"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1128\/JVI.01680-07","article-title":"JC virus agnoprotein inhibits in vitro differentiation of oligodendrocytes and promotes apoptosis","volume":"82","author":"N Merabova","year":"2008","journal-title":"J Virol"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.virol.2008.11.017","article-title":"Papillomavirus E6 proteins","volume":"384","author":"HL Howie","year":"2009","journal-title":"Virology"},{"issue":"43","key":"ref39","doi-asserted-by":"crossref","first-page":"36088","DOI":"10.1074\/jbc.M505138200","article-title":"Nucleo-cytoplasmic shuttling of high risk human Papillomavirus E2 proteins induces apoptosis","volume":"280","author":"S Blachon","year":"2005","journal-title":"J Biol Chem"},{"key":"ref40","article-title":"Center for Drug Evaluation and Research","year":"2006"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0200043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T04:21:00Z","timestamp":1605241260000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0200043"}},"subtitle":[],"editor":[{"given":"Edward","family":"Gershburg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,10,17]]},"references-count":40,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0200043","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/351825","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,17]]}}}