{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T13:00:45Z","timestamp":1773234045077,"version":"3.50.1"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T00:00:00Z","timestamp":1532390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["101842\/Z13\/Z"],"award-info":[{"award-number":["101842\/Z13\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["093712\/Z\/10\/Z"],"award-info":[{"award-number":["093712\/Z\/10\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0201263","type":"journal-article","created":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T17:42:26Z","timestamp":1532454146000},"page":"e0201263","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["The mRNA cap methyltransferase gene TbCMT1 is not essential in vitro but is a virulence factor in vivo for bloodstream form Trypanosoma brucei"],"prefix":"10.1371","volume":"13","author":[{"given":"Anna","family":"Kelner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0051-017X","authenticated-orcid":true,"given":"Michele","family":"Tinti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lucia S.","family":"Guther","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo J.","family":"Foth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lia","family":"Chappell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Berriman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria Haigh","family":"Cowling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1321-8714","authenticated-orcid":true,"given":"Michael A. J.","family":"Ferguson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,7,24]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.idc.2004.01.004","article-title":"tory of sleeping sickness (African trypanosomiasis)","volume":"18","author":"FE Cox","year":"2004","journal-title":"Infectious disease clinics of North America"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0166-6851(99)00157-7","article-title":"The use of transgenic Trypanosoma brucei to identify compounds inducing the differentiation of bloodstream forms to procyclic forms","volume":"104","author":"S Sbicego","year":"1999","journal-title":"Molecular and biochemical parasitology"},{"issue":"Pt 5","key":"ref3","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1017\/S0031182099004217","article-title":"Trypanosoma brucei spp. development in the tsetse fly: characterization of the post-mesocyclic stages in the foregut and proboscis","volume":"118","author":"J Van Den Abbeele","year":"1999","journal-title":"Parasitology"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0092-8674(87)90154-1","article-title":"Inactivation of transcription by UV irradiation of T. brucei provides evidence for a multicistronic transcription unit including a VSG gene","volume":"51","author":"PJ Johnson","year":"1987","journal-title":"Cell"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0166-6851(89)90108-4","article-title":"A transcriptional analysis of the Trypanosoma brucei hsp83 gene cluster","volume":"37","author":"JC Mottram","year":"1989","journal-title":"Molecular and biochemical parasitology"},{"issue":"6","key":"ref6","doi-asserted-by":"crossref","first-page":"3180","DOI":"10.1128\/MCB.11.6.3180","article-title":"Maturation of polycistronic pre-mRNA in Trypanosoma brucei: analysis of trans splicing and poly(A) addition at nascent RNA transcripts from the hsp70 locus","volume":"11","author":"J Huang","year":"1991","journal-title":"Molecular and cellular biology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"525241","DOI":"10.1155\/2010\/525241","article-title":"Gene expression in trypanosomatid parasites","volume":"2010","author":"S Martinez-Calvillo","year":"2010","journal-title":"Journal of biomedicine & biotechnology"},{"issue":"7","key":"ref8","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1093\/nar\/29.7.1556","article-title":"Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms","volume":"29","author":"G Gilinger","year":"2001","journal-title":"Nucleic acids research"},{"issue":"5599","key":"ref9","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/266235a0","article-title":"5'-Terminal structure and mRNA stability","volume":"266","author":"Y Furuichi","year":"1977","journal-title":"Nature"},{"issue":"23","key":"ref10","doi-asserted-by":"crossref","first-page":"9861","DOI":"10.1093\/nar\/15.23.9861","article-title":"Direct analysis of the mini-exon donor RNA of Trypanosoma brucei: detection of a novel cap structure also present in messenger RNA","volume":"15","author":"MS Freistadt","year":"1987","journal-title":"Nucleic acids research"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/0092-8674(86)90617-3","article-title":"Evidence for trans splicing in trypanosomes","volume":"47","author":"RE Sutton","year":"1986","journal-title":"Cell"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.molbiopara.2007.05.008","article-title":"Characterization of the Trypanosoma brucei cap hypermethylase Tgs1","volume":"155","author":"JP Ruan","year":"2007","journal-title":"Molecular and biochemical parasitology"},{"issue":"22","key":"ref13","doi-asserted-by":"crossref","first-page":"15995","DOI":"10.1074\/jbc.M701569200","article-title":"Trypanosoma brucei encodes a bifunctional capping enzyme essential for cap 4 formation on the spliced leader RNA","volume":"282","author":"Y Takagi","year":"2007","journal-title":"The Journal of biological chemistry"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1261\/rna.2250606","article-title":"Characterization of a Trypanosoma brucei RNA cap (guanine N-7) methyltransferase","volume":"12","author":"MP Hall","year":"2006","journal-title":"Rna"},{"issue":"8","key":"ref15","doi-asserted-by":"crossref","first-page":"4612","DOI":"10.1128\/MCB.18.8.4612","article-title":"Trypanosome capping enzymes display a novel two-domain structure","volume":"18","author":"E Silva","year":"1998","journal-title":"Molecular and cellular biology"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1021\/pr400086y","article-title":"Global quantitative SILAC phosphoproteomics reveals differential phosphorylation is widespread between the procyclic and bloodstream form lifecycle stages of Trypanosoma brucei","volume":"12","author":"MD Urbaniak","year":"2013","journal-title":"Journal of proteome research"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0166-6851(99)00002-X","article-title":"A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei","volume":"99","author":"E Wirtz","year":"1999","journal-title":"Molecular and biochemical parasitology"},{"issue":"17","key":"ref18","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1038\/emboj.2009.203","article-title":"Distinct donor and acceptor specificities of Trypanosoma brucei oligosaccharyltransferases","volume":"28","author":"L Izquierdo","year":"2009","journal-title":"The EMBO journal"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"6156","DOI":"10.1038\/s41598-017-06501-8","article-title":"RNAi screening identifies Trypanosoma brucei stress response protein kinases required for survival in the mouse","volume":"7","author":"F Fernandez-Cortes","year":"2017","journal-title":"Scientific reports"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1365-2958.2012.08026.x","article-title":"The de novo and salvage pathways of GDP-mannose biosynthesis are both sufficient for the growth of bloodstream-form Trypanosoma brucei","volume":"84","author":"S Kuettel","year":"2012","journal-title":"Molecular microbiology"},{"issue":"12","key":"ref21","doi-asserted-by":"crossref","first-page":"5265","DOI":"10.1091\/mbc.e06-08-0702","article-title":"GPI-anchored proteins and free GPI glycolipids of procyclic form Trypanosoma brucei are nonessential for growth, are required for colonization of the tsetse fly, and are not the only components of the surface coat","volume":"17","author":"ML Guther","year":"2006","journal-title":"Molecular biology of the cell"},{"issue":"5","key":"ref22","first-page":"734","article-title":"CDK1-Cyclin B1 Activates RNMT, Coordinating mRNA Cap Methylation with G1","volume":"61","author":"M Aregger","year":"2016","journal-title":"Phase Transcription. Molecular cell"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1016\/j.celrep.2016.06.089","article-title":"mRNA Cap Methylation in Pluripotency and Differentiation","volume":"16","author":"L Grasso","year":"2016","journal-title":"Cell reports"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"e36619","DOI":"10.1371\/journal.pone.0036619","article-title":"Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages","volume":"7","author":"MD Urbaniak","year":"2012","journal-title":"PloS one"},{"issue":"17","key":"ref25","doi-asserted-by":"crossref","first-page":"4923","DOI":"10.1093\/emboj\/20.17.4923","article-title":"Purification, cloning and characterization of a GPI inositol deacylase from Trypanosoma brucei","volume":"20","author":"ML Guther","year":"2001","journal-title":"The EMBO journal"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"748206","DOI":"10.1155\/2012\/748206","article-title":"Host-parasite interaction: parasite-derived and -induced proteases that degrade human extracellular matrix","volume":"2012","author":"C Pina-Vazquez","year":"2012","journal-title":"Journal of parasitology research"},{"issue":"5","key":"ref27","doi-asserted-by":"crossref","first-page":"e1004130","DOI":"10.1371\/journal.ppat.1004130","article-title":"Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei","volume":"10","author":"S Alsford","year":"2014","journal-title":"PLoS pathogens"},{"issue":"6","key":"ref28","doi-asserted-by":"crossref","first-page":"3190","DOI":"10.1128\/IAI.71.6.3190-3195.2003","article-title":"Expression of multiple CPB genes encoding cysteine proteases is required for Leishmania mexicana virulence in vivo","volume":"71","author":"H Denise","year":"2003","journal-title":"Infection and immunity"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.mib.2004.06.010","article-title":"Cysteine peptidases as virulence factors of Leishmania","volume":"7","author":"JC Mottram","year":"2004","journal-title":"Current opinion in microbiology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkp851","article-title":"TriTrypDB: a functional genomic resource for the Trypanosomatidae","volume":"38","author":"M Aslett","year":"2010","journal-title":"Nucleic acids research"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.molbiopara.2007.02.008","article-title":"Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei","volume":"153","author":"G Burkard","year":"2007","journal-title":"Molecular and biochemical parasitology"},{"key":"ref32","author":"J FE Sambrook","year":"1989"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.molbiopara.2014.06.004","article-title":"Capturing the variant surface glycoprotein repertoire (the VSGnome) of Trypanosoma brucei Lister 427","volume":"195","author":"GA Cross","year":"2014","journal-title":"Molecular and biochemical parasitology"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nature methods"},{"issue":"16","key":"ref35","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"H Li","year":"2009","journal-title":"Bioinformatics"},{"issue":"10","key":"ref36","doi-asserted-by":"crossref","first-page":"e108","DOI":"10.1093\/nar\/gkt214","article-title":"The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote","volume":"41","author":"Y Liao","year":"2013","journal-title":"Nucleic acids research"},{"issue":"12","key":"ref37","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome biology"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"D19","DOI":"10.1093\/nar\/gkq1019","article-title":"International Nucleotide Sequence Database C. The sequence read archive","volume":"39","author":"R Leinonen","year":"2011","journal-title":"Nucleic acids research"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0201263","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T01:26:46Z","timestamp":1540344406000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0201263"}},"subtitle":[],"editor":[{"given":"Juan","family":"Mata","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,7,24]]},"references-count":38,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2018,7,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0201263","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,24]]}}}